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The map label for this gene is fruB [H]

Identifier: 87200426

GI number: 87200426

Start: 2585441

End: 2587951

Strand: Reverse

Name: fruB [H]

Synonym: Saro_2413

Alternate gene names: 87200426

Gene position: 2587951-2585441 (Counterclockwise)

Preceding gene: 87200427

Following gene: 87200425

Centisome position: 72.66

GC content: 69.1

Gene sequence:

>2511_bases
ATGATGGGGTCGATTGTTCTCTCTGCGCCGATTGCAGGTTGGGTCTCCGCGCTCGACGAGGTCCCTGACGGCGTCTTCTC
CGCCCGCCTTCTGGGCGATGGCGTGGCCATCGATCCGGTCGAAGGCCTGCTCTGCGCGCCTTGCGATGGCGAAATCCTTT
CCGTCCACGCCGCGCGCCATGCCGTCACCATGGCAGGGGAGGGCGGCGTCGAACTGCTCATGCACCTCGGCATCGACACG
GTCGAGTTGAAGGGCGATGGCTTCGAGACGCTGGTCCGGGCAGGCCATCGCGTCGTGCGAGGGCAGCCTCTGCTGCGCTT
CTCGCTCGACGACCTTGCCGGCCGCGCGCCCTCGCTCGTCTCGCCGGTCATCGTCACCAATGGCGACCGTTTCACGATTT
CCTCCTGCACTGTCGACGCGCTCGTTGCCGTCGGAGACGACCTGATCCGGCTCGATCCCGTCGGAGTCGCCCCTTCGCCG
GATCGCGCGCCGGTGGGCGAGGCGGTCGGGGCCATTGTCGTCGTGCCCCTGCCCAACGGCCTTCACGCCCGGCCCGCCGC
GCGCCTGGGCGAAGCGGCGCGCGCCTTCGCCGCGGAAACGCGCATCGTGAAGAACGGCCGGGCGGTTTCCACCCGCTCGC
CGGTCGGCCTGCTCGGTCTGTCCATCCAGCTGGGCGACGAAATTCTCGTCGAGGCCAGCGGACCGGACGCCTCACAGGCA
GTGGCTGCGCTGGTCGACCTCATCCGCAGTGGGCTGGGCGAGGGCGCTGAACACGATGCCGCCGCAGAGCCGCGCGCCTC
GTCTGCCGGCATGCCGCCCTTCGCTCCGGTGCCGGATGGCGCGCTGCGCGGCAATCTTGCCTCGCCCGGCTTCGCGCTCG
GCACCTGCCACCGCCTCGACCGCTCCGAACCGGAACTGCCGGTGCAGGGAAAGGGCAAGGAAGAGGAGCGCATTCGCCTG
CTCTCGGCGATGGACCATCTGCGGTCGCGGCTCGCGATCTCGTCCGAGGTCAGCCCTAAGGCAGCGATATGCAGCGCTCA
CCGCGCGCTGCTGGACGATCCCGAACTCGAAGCCGCGGCCCTTTCGCGTATCGCCGAGGGCGAAGACGCCGCCCATGCCT
GGCGCGAGGCTTGCCGCGCTTCGGCGGTGGTTCTGCGCGGCAGCGGTTCGGCCCGCTTTGCCGAGCGCGCGGACGACGTT
CTCGACCTCGGGAGGCAGCTCGTCACCATCCTCATCGGCGAACTCGACGAGACGTTGGCGTTTCCTCCGGGGACGATCCT
CCTGGCAGACGAACTGCTGCCTTCCCAGATCATGCAGCTCGGCGCCGAGGTTACCGGCATCGCGCTCGCGAACGGTGGCC
CGACCTCGCATGTCGCGATCCTTGCGGCCAGCATGGGCATTCCAATGCTTGTCGCCATCGGCGATGCGCTCGCAGGCGTT
CGCGCGGGCGACAAGGCCATTCTCGATGCCGACGGCGGCTTTCTCCTCCCGGCGCCCGGCGCTGCCGCGCTTGCCGAGGC
AGAAGCGGAAGTTGCCGCGCGTCAGGCGAGGCGCGCCGATGCCCTCGCCAGCGCGAGCGAGCTTTGCCACAGCCGCGACG
GCGCGCGCATCGAAGTCTTCGCCAATCTCGGCTCGCTCGACGATGCCCGTCGGGCAGTCTCCGCCGGGGCCGAGGGCTGC
GGATTGCTGCGCACGGAATTCCTGTTTCTCGAAAGGGAAAGCGCGCCGACCGTCGCCCAGCAGGCCGAACTCTACGCCGG
GATTGCCGATGCGCTGGGCGGTCGCCCCCTGATCGTCCGCCTGCTCGACATCGGCGGTGACAAGCCCGCAACCTATCTGC
CCATCGCGCCAGAGGCCAATCCGGCACTCGGTTTGCGCGGCATCCGGGTGGGACTTGCCCATCCCGATGTGCTGGAAGAC
CAGTTGCGCGCCATTCTTTCGGTCGAACGCGGAGGGGCGCTGCGGATCATGGTGCCGATGGTCACCGGCGTCGCCGAGGT
CCGCGAGGTGCGCCAGCGGGTCGACCGGATCAGCGCCGAACTTGGCCTCGCGCAGCGCGTCGAAGTCGGCATCATGGTCG
AAACGCCTGCCGCCGCCGCCACCGCGTTCCTGCTCGCGCCGCACGCCGATTTCATGTCCATCGGCACGAACGACCTGACC
CAGTACGTCCTCGCGATGGACCGGGACAATCCGGCCGTGGCGGGTGGGATCGACGGGCTCCACCCGGCCGTGCTCAACCT
GATCGCGCAGACCGTGCGCGGCGCGCAATCGGTCGGTCGCTGGACCGGAGTTTGCGGCGGACTTGCGGCAGATCGCCTTG
CGGTGCCGCTGCTCCTCGGCCTCGGCGTGACCGAGCTGTCGGTGCCGCTGCGTCAATTGCCCGAAATCAAGGCGCTCGTG
CGCGGGCTGTCCATCTCGGACTGCAAGACTCTCGCCCTCGAGGCGCTTCAGCTTGAATCCGCTGCCGAAATCCGTGCCCT
GTCGCGGGCATTCGTGGAGACACTGCCATGA

Upstream 100 bases:

>100_bases
GATTTCCGCACCCAGGCCACCTGGATCGGCGGGCAGCGGGTACGCTGACGCAGCTTGCCGCCCCGTCGCGCCGCGTGGCA
GCCGGGGATGGAGGGAAGAA

Downstream 100 bases:

>100_bases
GGAACCTTCTCGGCCTGCTCCAGCCGATCGGTCGCGCGCTGATGCTGCCGATCGCGGTGCTCCCCGTCGCGGGGCTTCTG
CTGCGCCTCGGCCAGCCCGA

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 836; Mature: 836

Protein sequence:

>836_residues
MMGSIVLSAPIAGWVSALDEVPDGVFSARLLGDGVAIDPVEGLLCAPCDGEILSVHAARHAVTMAGEGGVELLMHLGIDT
VELKGDGFETLVRAGHRVVRGQPLLRFSLDDLAGRAPSLVSPVIVTNGDRFTISSCTVDALVAVGDDLIRLDPVGVAPSP
DRAPVGEAVGAIVVVPLPNGLHARPAARLGEAARAFAAETRIVKNGRAVSTRSPVGLLGLSIQLGDEILVEASGPDASQA
VAALVDLIRSGLGEGAEHDAAAEPRASSAGMPPFAPVPDGALRGNLASPGFALGTCHRLDRSEPELPVQGKGKEEERIRL
LSAMDHLRSRLAISSEVSPKAAICSAHRALLDDPELEAAALSRIAEGEDAAHAWREACRASAVVLRGSGSARFAERADDV
LDLGRQLVTILIGELDETLAFPPGTILLADELLPSQIMQLGAEVTGIALANGGPTSHVAILAASMGIPMLVAIGDALAGV
RAGDKAILDADGGFLLPAPGAAALAEAEAEVAARQARRADALASASELCHSRDGARIEVFANLGSLDDARRAVSAGAEGC
GLLRTEFLFLERESAPTVAQQAELYAGIADALGGRPLIVRLLDIGGDKPATYLPIAPEANPALGLRGIRVGLAHPDVLED
QLRAILSVERGGALRIMVPMVTGVAEVREVRQRVDRISAELGLAQRVEVGIMVETPAAAATAFLLAPHADFMSIGTNDLT
QYVLAMDRDNPAVAGGIDGLHPAVLNLIAQTVRGAQSVGRWTGVCGGLAADRLAVPLLLGLGVTELSVPLRQLPEIKALV
RGLSISDCKTLALEALQLESAAEIRALSRAFVETLP

Sequences:

>Translated_836_residues
MMGSIVLSAPIAGWVSALDEVPDGVFSARLLGDGVAIDPVEGLLCAPCDGEILSVHAARHAVTMAGEGGVELLMHLGIDT
VELKGDGFETLVRAGHRVVRGQPLLRFSLDDLAGRAPSLVSPVIVTNGDRFTISSCTVDALVAVGDDLIRLDPVGVAPSP
DRAPVGEAVGAIVVVPLPNGLHARPAARLGEAARAFAAETRIVKNGRAVSTRSPVGLLGLSIQLGDEILVEASGPDASQA
VAALVDLIRSGLGEGAEHDAAAEPRASSAGMPPFAPVPDGALRGNLASPGFALGTCHRLDRSEPELPVQGKGKEEERIRL
LSAMDHLRSRLAISSEVSPKAAICSAHRALLDDPELEAAALSRIAEGEDAAHAWREACRASAVVLRGSGSARFAERADDV
LDLGRQLVTILIGELDETLAFPPGTILLADELLPSQIMQLGAEVTGIALANGGPTSHVAILAASMGIPMLVAIGDALAGV
RAGDKAILDADGGFLLPAPGAAALAEAEAEVAARQARRADALASASELCHSRDGARIEVFANLGSLDDARRAVSAGAEGC
GLLRTEFLFLERESAPTVAQQAELYAGIADALGGRPLIVRLLDIGGDKPATYLPIAPEANPALGLRGIRVGLAHPDVLED
QLRAILSVERGGALRIMVPMVTGVAEVREVRQRVDRISAELGLAQRVEVGIMVETPAAAATAFLLAPHADFMSIGTNDLT
QYVLAMDRDNPAVAGGIDGLHPAVLNLIAQTVRGAQSVGRWTGVCGGLAADRLAVPLLLGLGVTELSVPLRQLPEIKALV
RGLSISDCKTLALEALQLESAAEIRALSRAFVETLP
>Mature_836_residues
MMGSIVLSAPIAGWVSALDEVPDGVFSARLLGDGVAIDPVEGLLCAPCDGEILSVHAARHAVTMAGEGGVELLMHLGIDT
VELKGDGFETLVRAGHRVVRGQPLLRFSLDDLAGRAPSLVSPVIVTNGDRFTISSCTVDALVAVGDDLIRLDPVGVAPSP
DRAPVGEAVGAIVVVPLPNGLHARPAARLGEAARAFAAETRIVKNGRAVSTRSPVGLLGLSIQLGDEILVEASGPDASQA
VAALVDLIRSGLGEGAEHDAAAEPRASSAGMPPFAPVPDGALRGNLASPGFALGTCHRLDRSEPELPVQGKGKEEERIRL
LSAMDHLRSRLAISSEVSPKAAICSAHRALLDDPELEAAALSRIAEGEDAAHAWREACRASAVVLRGSGSARFAERADDV
LDLGRQLVTILIGELDETLAFPPGTILLADELLPSQIMQLGAEVTGIALANGGPTSHVAILAASMGIPMLVAIGDALAGV
RAGDKAILDADGGFLLPAPGAAALAEAEAEVAARQARRADALASASELCHSRDGARIEVFANLGSLDDARRAVSAGAEGC
GLLRTEFLFLERESAPTVAQQAELYAGIADALGGRPLIVRLLDIGGDKPATYLPIAPEANPALGLRGIRVGLAHPDVLED
QLRAILSVERGGALRIMVPMVTGVAEVREVRQRVDRISAELGLAQRVEVGIMVETPAAAATAFLLAPHADFMSIGTNDLT
QYVLAMDRDNPAVAGGIDGLHPAVLNLIAQTVRGAQSVGRWTGVCGGLAADRLAVPLLLGLGVTELSVPLRQLPEIKALV
RGLSISDCKTLALEALQLESAAEIRALSRAFVETLP

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=582, Percent_Identity=36.426116838488, Blast_Score=317, Evalue=2e-87,
Organism=Escherichia coli, GI1788726, Length=686, Percent_Identity=33.3819241982507, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI48994992, Length=690, Percent_Identity=32.1739130434783, Blast_Score=281, Evalue=9e-77,
Organism=Escherichia coli, GI1789193, Length=500, Percent_Identity=32.6, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI226510935, Length=335, Percent_Identity=31.044776119403, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1786894, Length=126, Percent_Identity=43.6507936507937, Blast_Score=115, Evalue=2e-26,
Organism=Escherichia coli, GI1788757, Length=127, Percent_Identity=40.9448818897638, Blast_Score=110, Evalue=5e-25,
Organism=Escherichia coli, GI1787994, Length=403, Percent_Identity=26.5508684863524, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1790159, Length=126, Percent_Identity=37.3015873015873, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 86311; Mature: 86311

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00369 PTS_HPR_HIS ; PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS00742 PEP_ENZYMES_2 ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMGSIVLSAPIAGWVSALDEVPDGVFSARLLGDGVAIDPVEGLLCAPCDGEILSVHAARH
CCCCEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCEECCCCCEEECCCCCCEEEEEHHHH
AVTMAGEGGVELLMHLGIDTVELKGDGFETLVRAGHRVVRGQPLLRFSLDDLAGRAPSLV
EEEECCCCHHHHHHHCCCCEEEECCCCHHHHHHCCCHHHCCCCEEEEEHHHHCCCCCCCC
SPVIVTNGDRFTISSCTVDALVAVGDDLIRLDPVGVAPSPDRAPVGEAVGAIVVVPLPNG
CCEEEECCCEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHCCEEEEEECCCC
LHARPAARLGEAARAFAAETRIVKNGRAVSTRSPVGLLGLSIQLGDEILVEASGPDASQA
CCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEEECCEEEEECCCCCHHHH
VAALVDLIRSGLGEGAEHDAAAEPRASSAGMPPFAPVPDGALRGNLASPGFALGTCHRLD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHCC
RSEPELPVQGKGKEEERIRLLSAMDHLRSRLAISSEVSPKAAICSAHRALLDDPELEAAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHH
LSRIAEGEDAAHAWREACRASAVVLRGSGSARFAERADDVLDLGRQLVTILIGELDETLA
HHHHHCCCHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPPGTILLADELLPSQIMQLGAEVTGIALANGGPTSHVAILAASMGIPMLVAIGDALAGV
CCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEHHHHHHHCC
RAGDKAILDADGGFLLPAPGAAALAEAEAEVAARQARRADALASASELCHSRDGARIEVF
CCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ANLGSLDDARRAVSAGAEGCGLLRTEFLFLERESAPTVAQQAELYAGIADALGGRPLIVR
ECCCCCHHHHHHHHCCCCCCCHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEE
LLDIGGDKPATYLPIAPEANPALGLRGIRVGLAHPDVLEDQLRAILSVERGGALRIMVPM
EEECCCCCCCEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHH
VTGVAEVREVRQRVDRISAELGLAQRVEVGIMVETPAAAATAFLLAPHADFMSIGTNDLT
HHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCHHHHHHHHCCCCCHHHHCCCHHHH
QYVLAMDRDNPAVAGGIDGLHPAVLNLIAQTVRGAQSVGRWTGVCGGLAADRLAVPLLLG
HHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGVTELSVPLRQLPEIKALVRGLSISDCKTLALEALQLESAAEIRALSRAFVETLP
CCHHHHHCCHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMGSIVLSAPIAGWVSALDEVPDGVFSARLLGDGVAIDPVEGLLCAPCDGEILSVHAARH
CCCCEEECCHHHHHHHHHHHCCCHHHHHHHHCCCCEECCCCCEEECCCCCCEEEEEHHHH
AVTMAGEGGVELLMHLGIDTVELKGDGFETLVRAGHRVVRGQPLLRFSLDDLAGRAPSLV
EEEECCCCHHHHHHHCCCCEEEECCCCHHHHHHCCCHHHCCCCEEEEEHHHHCCCCCCCC
SPVIVTNGDRFTISSCTVDALVAVGDDLIRLDPVGVAPSPDRAPVGEAVGAIVVVPLPNG
CCEEEECCCEEEEEHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHCCEEEEEECCCC
LHARPAARLGEAARAFAAETRIVKNGRAVSTRSPVGLLGLSIQLGDEILVEASGPDASQA
CCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEEEECCEEEEECCCCCHHHH
VAALVDLIRSGLGEGAEHDAAAEPRASSAGMPPFAPVPDGALRGNLASPGFALGTCHRLD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHCC
RSEPELPVQGKGKEEERIRLLSAMDHLRSRLAISSEVSPKAAICSAHRALLDDPELEAAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHH
LSRIAEGEDAAHAWREACRASAVVLRGSGSARFAERADDVLDLGRQLVTILIGELDETLA
HHHHHCCCHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
FPPGTILLADELLPSQIMQLGAEVTGIALANGGPTSHVAILAASMGIPMLVAIGDALAGV
CCCCCEEEEHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEHHHHHHHCC
RAGDKAILDADGGFLLPAPGAAALAEAEAEVAARQARRADALASASELCHSRDGARIEVF
CCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
ANLGSLDDARRAVSAGAEGCGLLRTEFLFLERESAPTVAQQAELYAGIADALGGRPLIVR
ECCCCCHHHHHHHHCCCCCCCHHHHHEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEE
LLDIGGDKPATYLPIAPEANPALGLRGIRVGLAHPDVLEDQLRAILSVERGGALRIMVPM
EEECCCCCCCEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEHHHH
VTGVAEVREVRQRVDRISAELGLAQRVEVGIMVETPAAAATAFLLAPHADFMSIGTNDLT
HHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEEECCHHHHHHHHCCCCCHHHHCCCHHHH
QYVLAMDRDNPAVAGGIDGLHPAVLNLIAQTVRGAQSVGRWTGVCGGLAADRLAVPLLLG
HHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LGVTELSVPLRQLPEIKALVRGLSISDCKTLALEALQLESAAEIRALSRAFVETLP
CCHHHHHCCHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496537; 12024217; 1655739 [H]