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The map label for this gene is 87200422

Identifier: 87200422

GI number: 87200422

Start: 2576621

End: 2577754

Strand: Reverse

Name: 87200422

Synonym: Saro_2409

Alternate gene names: NA

Gene position: 2577754-2576621 (Counterclockwise)

Preceding gene: 87200424

Following gene: 87200421

Centisome position: 72.38

GC content: 66.14

Gene sequence:

>1134_bases
ATGGCGAAGATCAAGACCACTCATGTCGGAAGCCTGCCGCGCGGCGACGAACTGACGCCGTTGCTCCTGGCCCGCGACAA
GGGCGAACCCTATGACGCCGCCGAGTTCGACCGCGTCGTTTCCGCCGCCGTCGACCAAGCGGTGAAGGACCAGGTTGCAG
CGGGGGTCTCGGTCGTGTCCGATGGCGAACTCGGCAAGGTCGGTTACTCGACCTACATGATCGAGCGCCTGTCCGGCTTC
GGCGGCCACATCGATCGCAAGCCCGCGGCCGACCTCGCCGAAGTGCCGAACCTGGCGAAGAAGCTGTCGGCGATCATGGG
CAGCCAGGAATTCGTCCGCGCCTCGTGCATCGGGCCGGTGCGCAAGGTCACCCTCCAGCCGCTGCACGACGATATCCGCC
GCTTCAAGGCCGCGCTGGCGAACCACGCCGCGCCCGATACCGAGGCGTTCATGAACGCGGCGTCGCCGGGTCTCATCACC
GCCTTCCAGGTCAACCGCCACTACCCGAGCCACGAGGCCTACCTCGCAGACCTCGCCGATGCGATGCGCGAGGAGTACGA
GACGATCGTGAACGAGGGCTTCTACCTCCAGCTCGATTGCCCCGACCTCGCGATGAGCCGCCACACCGGCTATCAGGACC
TCTCGGAAGAGGATTTCCTCAAGGTCGCGGCCGCCAACGTCGAGGCGCTGAACGCCGCCACCGCGAACATCCCGCCGGAA
CGGATGCGGATGCACATCTGCTGGGGCAACTACGAAGGCCCGCACGACCACGACATCCCGCTCGAGCGCGTCGTCGACAT
CATCATCAAGGCGCGGCCGGCGACGATCCTGTTCGAAGCCGCCAATCCGCGCCACGAGCACGAATGGAAGGTGTGGAAGG
ACGCGAAGCTGCCCGACCACAAGATCCTCGCTCCGGGCCTGATCGACACCTGCTCCAACTACATCGAGCATCCCGAACTG
ATCGCCCAGCGCATCGAGCGCTTCGCCAATTTCGTCGGCGCGGACCGCGTCGTTGCCAGCACCGACTGCGGCTTCGGCAC
CTTCGCGGGCTATGGCAAGCTCGATCCCATCGTCACGTGGCGCAAGCTCCGGTCGCTGCGGGAAGGCGCGGATATCGCCG
CGAGCCGCCTGTGA

Upstream 100 bases:

>100_bases
CGGCGGCGAAACTGCGCAAAAAGCCCGCGTCCGGCTTGTTTCGGCGTCGCGAACGCTTATGAAGTTTTCACGGTTATGAA
TTGAGAGAGGCGTGTCCCCG

Downstream 100 bases:

>100_bases
GGCCGGAAGACCGCCGCGCCGCCGAGCATGATTGCGCCCGCCTGATCGCGCTCTACGCCAACCTCAACGACGAGGCGCGC
TGGGACGAGGTTGCGGCCCT

Product: methionine synthase, vitamin-B12 independent

Products: tetrahydropteroyltri-L-glutamate; L-methionine

Alternate protein names: Methionine Synthase II; Methionine Synthase II- Like Protein; EpoxyalkaneCo M Transferase; Methyltransferase; Methionine Synthase Vitamin-B12 Independent Protein

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MAKIKTTHVGSLPRGDELTPLLLARDKGEPYDAAEFDRVVSAAVDQAVKDQVAAGVSVVSDGELGKVGYSTYMIERLSGF
GGHIDRKPAADLAEVPNLAKKLSAIMGSQEFVRASCIGPVRKVTLQPLHDDIRRFKAALANHAAPDTEAFMNAASPGLIT
AFQVNRHYPSHEAYLADLADAMREEYETIVNEGFYLQLDCPDLAMSRHTGYQDLSEEDFLKVAAANVEALNAATANIPPE
RMRMHICWGNYEGPHDHDIPLERVVDIIIKARPATILFEAANPRHEHEWKVWKDAKLPDHKILAPGLIDTCSNYIEHPEL
IAQRIERFANFVGADRVVASTDCGFGTFAGYGKLDPIVTWRKLRSLREGADIAASRL

Sequences:

>Translated_377_residues
MAKIKTTHVGSLPRGDELTPLLLARDKGEPYDAAEFDRVVSAAVDQAVKDQVAAGVSVVSDGELGKVGYSTYMIERLSGF
GGHIDRKPAADLAEVPNLAKKLSAIMGSQEFVRASCIGPVRKVTLQPLHDDIRRFKAALANHAAPDTEAFMNAASPGLIT
AFQVNRHYPSHEAYLADLADAMREEYETIVNEGFYLQLDCPDLAMSRHTGYQDLSEEDFLKVAAANVEALNAATANIPPE
RMRMHICWGNYEGPHDHDIPLERVVDIIIKARPATILFEAANPRHEHEWKVWKDAKLPDHKILAPGLIDTCSNYIEHPEL
IAQRIERFANFVGADRVVASTDCGFGTFAGYGKLDPIVTWRKLRSLREGADIAASRL
>Mature_376_residues
AKIKTTHVGSLPRGDELTPLLLARDKGEPYDAAEFDRVVSAAVDQAVKDQVAAGVSVVSDGELGKVGYSTYMIERLSGFG
GHIDRKPAADLAEVPNLAKKLSAIMGSQEFVRASCIGPVRKVTLQPLHDDIRRFKAALANHAAPDTEAFMNAASPGLITA
FQVNRHYPSHEAYLADLADAMREEYETIVNEGFYLQLDCPDLAMSRHTGYQDLSEEDFLKVAAANVEALNAATANIPPER
MRMHICWGNYEGPHDHDIPLERVVDIIIKARPATILFEAANPRHEHEWKVWKDAKLPDHKILAPGLIDTCSNYIEHPELI
AQRIERFANFVGADRVVASTDCGFGTFAGYGKLDPIVTWRKLRSLREGADIAASRL

Specific function: Unknown

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.14

Molecular weight: Translated: 41535; Mature: 41403

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKTTHVGSLPRGDELTPLLLARDKGEPYDAAEFDRVVSAAVDQAVKDQVAAGVSVVS
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEC
DGELGKVGYSTYMIERLSGFGGHIDRKPAADLAEVPNLAKKLSAIMGSQEFVRASCIGPV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHH
RKVTLQPLHDDIRRFKAALANHAAPDTEAFMNAASPGLITAFQVNRHYPSHEAYLADLAD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHH
AMREEYETIVNEGFYLQLDCPDLAMSRHTGYQDLSEEDFLKVAAANVEALNAATANIPPE
HHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHCCCCCCCHH
RMRMHICWGNYEGPHDHDIPLERVVDIIIKARPATILFEAANPRHEHEWKVWKDAKLPDH
HEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCCCCC
KILAPGLIDTCSNYIEHPELIAQRIERFANFVGADRVVASTDCGFGTFAGYGKLDPIVTW
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHH
RKLRSLREGADIAASRL
HHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
AKIKTTHVGSLPRGDELTPLLLARDKGEPYDAAEFDRVVSAAVDQAVKDQVAAGVSVVS
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEC
DGELGKVGYSTYMIERLSGFGGHIDRKPAADLAEVPNLAKKLSAIMGSQEFVRASCIGPV
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHH
RKVTLQPLHDDIRRFKAALANHAAPDTEAFMNAASPGLITAFQVNRHYPSHEAYLADLAD
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHH
AMREEYETIVNEGFYLQLDCPDLAMSRHTGYQDLSEEDFLKVAAANVEALNAATANIPPE
HHHHHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHCCCCCCCHH
RMRMHICWGNYEGPHDHDIPLERVVDIIIKARPATILFEAANPRHEHEWKVWKDAKLPDH
HEEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCCCCC
KILAPGLIDTCSNYIEHPELIAQRIERFANFVGADRVVASTDCGFGTFAGYGKLDPIVTW
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHH
RKLRSLREGADIAASRL
HHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5-methyltetrahydropteroyltri-L-glutamate; L-homocysteine

Specific reaction: 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA