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The map label for this gene is mltB [H]

Identifier: 87199961

GI number: 87199961

Start: 2059285

End: 2060304

Strand: Reverse

Name: mltB [H]

Synonym: Saro_1944

Alternate gene names: 87199961

Gene position: 2060304-2059285 (Counterclockwise)

Preceding gene: 87199962

Following gene: 87199960

Centisome position: 57.85

GC content: 63.33

Gene sequence:

>1020_bases
TTGCGGGCTGGGCTGACAGCCGCCTTGGCGTTGGTTCTGGCGGGCCCCGCAGGTGCACAGGTCGATCAGGAAGAGGCTGG
TTTTCAAGGATACCTGCAACTTCTTTCGGCGCGTGCGCGGGGTGAAGGAGTCCGGGAAACCACGATTTCGGCGATGACGT
CCGGGCTCACGCTTAACCACCGTGTTATCGAGCTGGATCGCAGCCAGCCTGGGTCCGCGACAGGTCCGATTGCGAACTTC
GAACCCTATCGGCGCCAGCATGTCGACGCTGCGCGCATCGCGCGGGGCCGGCGCCAGTTCGCCGCCGCCAGTGGGCAACT
TGCGACGATCGAACGGCGTTATGGCGTACCAGGCCCGATCCTTCTGGCGATCTGGGGCCACGAAACAGACTACGGTGGCT
ACACTGGAAACTTCGATCTGCCGCGCGCGCTGGCGACCCTGGCCTATGAAGGGCGTCGGCGGGAGTTGTTTGCGGACGAA
TTCATCGCTGCCATGAAAATGGTGGACCGGGGCGTGCCGCGATCGAAGTTGGTGGGGAGCTGGGCCGGCGCATTCGGCAA
TCCGCAGTTCCTTCCTTCGGTCTATCTGCGCGTGGCGCAGGACGGGGATGGCGACGGCTTCGCGGATATCTGGACCAGCC
GCGCGGACACATTGGCGTCTATCGCCAACTATTTCCGCGATGCGGGATGGCGACCGGGCCAGCCCTGGGGCGTGGCGGTA
ACGGTGCCGGCGGACCTCGATCGCTCGAGCCTAGGTACGACGCTGCGGAGCCCGCGCTGTCCCCGGGTTTTCGAACGCCA
TTCGGGCTGGAAGACCATGCGCGAATGGCGCGCGCTGGGTATTGCGCCAAAGTTCGGGGCATGGCCAGGCGACGATGTGC
TGGCTAGTCTGCTCGAACCCGATGGGCAGGGCAGGACTGCCTACCTGCTAACCGGAAATTATAGGGTTATCCTCGATTAC
AACTGCTCGAACTTTTACGCTTTGTCCGTGGGACTTCTTGCAGATGAGATCAGCCGTTAG

Upstream 100 bases:

>100_bases
TTCGCCGTTAGCGCCTTCCACATCGGTCGCAAAGGGGATTCCCATCCGGAAGTATCCGTGCGAGACAGGTTTGATGATTT
TTTCGATGGGTAAGAGAGGT

Downstream 100 bases:

>100_bases
CCATATCGCGATTGCGACGACCCTGTTGCTTGCTGCCTGCGGCGGTGCAGACGCCCGCGTTTTCGGGCCGTCTGTCGACA
TCATTCGCGGGCCGGCGGGC

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MRAGLTAALALVLAGPAGAQVDQEEAGFQGYLQLLSARARGEGVRETTISAMTSGLTLNHRVIELDRSQPGSATGPIANF
EPYRRQHVDAARIARGRRQFAAASGQLATIERRYGVPGPILLAIWGHETDYGGYTGNFDLPRALATLAYEGRRRELFADE
FIAAMKMVDRGVPRSKLVGSWAGAFGNPQFLPSVYLRVAQDGDGDGFADIWTSRADTLASIANYFRDAGWRPGQPWGVAV
TVPADLDRSSLGTTLRSPRCPRVFERHSGWKTMREWRALGIAPKFGAWPGDDVLASLLEPDGQGRTAYLLTGNYRVILDY
NCSNFYALSVGLLADEISR

Sequences:

>Translated_339_residues
MRAGLTAALALVLAGPAGAQVDQEEAGFQGYLQLLSARARGEGVRETTISAMTSGLTLNHRVIELDRSQPGSATGPIANF
EPYRRQHVDAARIARGRRQFAAASGQLATIERRYGVPGPILLAIWGHETDYGGYTGNFDLPRALATLAYEGRRRELFADE
FIAAMKMVDRGVPRSKLVGSWAGAFGNPQFLPSVYLRVAQDGDGDGFADIWTSRADTLASIANYFRDAGWRPGQPWGVAV
TVPADLDRSSLGTTLRSPRCPRVFERHSGWKTMREWRALGIAPKFGAWPGDDVLASLLEPDGQGRTAYLLTGNYRVILDY
NCSNFYALSVGLLADEISR
>Mature_339_residues
MRAGLTAALALVLAGPAGAQVDQEEAGFQGYLQLLSARARGEGVRETTISAMTSGLTLNHRVIELDRSQPGSATGPIANF
EPYRRQHVDAARIARGRRQFAAASGQLATIERRYGVPGPILLAIWGHETDYGGYTGNFDLPRALATLAYEGRRRELFADE
FIAAMKMVDRGVPRSKLVGSWAGAFGNPQFLPSVYLRVAQDGDGDGFADIWTSRADTLASIANYFRDAGWRPGQPWGVAV
TVPADLDRSSLGTTLRSPRCPRVFERHSGWKTMREWRALGIAPKFGAWPGDDVLASLLEPDGQGRTAYLLTGNYRVILDY
NCSNFYALSVGLLADEISR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=301, Percent_Identity=27.906976744186, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36923; Mature: 36923

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGLTAALALVLAGPAGAQVDQEEAGFQGYLQLLSARARGEGVRETTISAMTSGLTLNH
CCCCHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEECC
RVIELDRSQPGSATGPIANFEPYRRQHVDAARIARGRRQFAAASGQLATIERRYGVPGPI
EEEEECCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCE
LLAIWGHETDYGGYTGNFDLPRALATLAYEGRRRELFADEFIAAMKMVDRGVPRSKLVGS
EEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
WAGAFGNPQFLPSVYLRVAQDGDGDGFADIWTSRADTLASIANYFRDAGWRPGQPWGVAV
HHCCCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
TVPADLDRSSLGTTLRSPRCPRVFERHSGWKTMREWRALGIAPKFGAWPGDDVLASLLEP
EECCCCCHHHHCCHHCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
DGQGRTAYLLTGNYRVILDYNCSNFYALSVGLLADEISR
CCCCCEEEEEECCEEEEEEECCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure
MRAGLTAALALVLAGPAGAQVDQEEAGFQGYLQLLSARARGEGVRETTISAMTSGLTLNH
CCCCHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEECC
RVIELDRSQPGSATGPIANFEPYRRQHVDAARIARGRRQFAAASGQLATIERRYGVPGPI
EEEEECCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCE
LLAIWGHETDYGGYTGNFDLPRALATLAYEGRRRELFADEFIAAMKMVDRGVPRSKLVGS
EEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
WAGAFGNPQFLPSVYLRVAQDGDGDGFADIWTSRADTLASIANYFRDAGWRPGQPWGVAV
HHCCCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
TVPADLDRSSLGTTLRSPRCPRVFERHSGWKTMREWRALGIAPKFGAWPGDDVLASLLEP
EECCCCCHHHHCCHHCCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCC
DGQGRTAYLLTGNYRVILDYNCSNFYALSVGLLADEISR
CCCCCEEEEEECCEEEEEEECCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]