Click here to switch to the map view.

The map label for this gene is metG [H]

Identifier: 87199956

GI number: 87199956

Start: 2053996

End: 2055561

Strand: Reverse

Name: metG [H]

Synonym: Saro_1939

Alternate gene names: 87199956

Gene position: 2055561-2053996 (Counterclockwise)

Preceding gene: 87199957

Following gene: 87199955

Centisome position: 57.71

GC content: 58.75

Gene sequence:

>1566_bases
ATGGGCGAACCCTTCTACATCACCACTGCCATCTCCTACCCCAACGGCAAGCCGCATATCGGCCATGCCTACGAGGCAAT
TGCCGCCGATGTTATCGCGCGCTTCCAACGGGCCATGGGACGTGATGTGCGTTTCCAGACGGGCACGGACGAACACGGGT
TGAAGATGGCGCAGAAGGCCCGTGAACTCGGCATAACGCCACGCGAGCTTTCCGACGAAATGTCATCTTATTTCATTAAG
ATGTGCGACGAACTTAATGTTTCGTACGACGTTTTCATTCGCACCACCGAGGAGCGGCATCACGCCTCGACGCAGGAACT
CTGGCGTCGCATGGAGGCCAATGGTGATCTCTATCTCGACCGCTACGAAGGCTGGTATTCGGTCCGGGACGAGGCTTTTT
ACGATGAGAGCGAACTGGTAGCGGGCGAGGGCGGGGAGAAGCTGTCGCCCCAGGGTACCCCGGTGGATTGGACAGTCGAG
GAAAGCTGGTTCTTCCGGCTTTCGAAATATGCCGAACCGCTACTGAAGCTCTACGAGGAGAATCCCGGGTTCATCCAGCC
CGACAGCCGCCGCAATGAAGTGATGCGCTTCGTCGAGGGTGGACTGCGTGACCTTTCGGTTTCTCGCACCAGCTTCGACT
GGGGTGTGAAGGTTCCGGGGTGCGATGGCCATGTAATGTACGTTTGGGTCGATGCTCTCACCAACTATATCACTGGGCTC
GGTTTTCCGGACGAAAACGGCGACTTTGCAAAGTATTGGCCGGCGAACCTGCACCTGATCGGCAAGGATATCGTCCGCTT
CCACACTGTCTACTGGCCGGCCTTCCTGATGAGCGCGGACATCGCGCTGCCGCGGCAAGTCTTCGGGCACGGATTCCTGC
TCAACCGTGGCCAGAAGGAATCGAAGTCGCTCGGTAACGTCACCGATCCACTCGACCTTGCCGACCGGTTCGGGGTAGAT
CCGCTTCGTTACTTCCTGATGCGGGAAGTAGCCTTCGGTCAGGACGGATCCTATTCGGCCGAGGCCATTGTGACGCGATG
CAATGCAGAGCTAGCAAACAGCTACGGCAATCTCGTTCAGCGCACACTATCCATGATTTTCAAAAACATGGGCGGCAATC
TTGAGACATTTCATAGCAATGTGGGGGACGACGAACTGCTGGCTACGGTGTTCAATGCGTGCCGTGAGGAACTGCCGCGC
GAGTTTTCCGCGCTGAACTTCTCGGCCGGGATCGAAGCTTGGATGCGTGCGGTCTTTGCCTGCAACGCCTATGTCGACGA
ACAGGCGCCGTGGGCGCTGCGCAAGACCGATCCCGAGCGCATGAAGGCCGTGCTGCTGACGCTGTTCATAGCGATCCGCG
ACCTGACCGTGGCGATTTCACCCGTCGTTCCGGCTGCCGCAGCCAAGGTGCTGGACCAGCTCGGCATTCCAAGGGAAGCG
CGGGGTTTCGATGCGTTGACTGATGCGGACTGGTACATGGCACGCGTGGCAACCGGAGAGAGGCTTGCGCAGCCCATGCC
TGCATTCCCCCGCCTTGAACTGCCGGAGGAGGAAGGATCGGCATGA

Upstream 100 bases:

>100_bases
ATGCTGATGTTGGAAATCGGCTCCTTGCTGGCATCGGCTGCGCCGACTAGGGAAGGCGCACGATAAGCATCAGATTCCGG
TTACGAAAGCGAGCGGCCCG

Downstream 100 bases:

>100_bases
TGCTGATCGATTCCCACTGCCACCTGAACTACAAGGGACTTGTCGAAGATCAGCGGCAAGTGCTTGACCGCGCCAGAGAA
AATGGCGTGCGTGGTTTCCT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MGEPFYITTAISYPNGKPHIGHAYEAIAADVIARFQRAMGRDVRFQTGTDEHGLKMAQKARELGITPRELSDEMSSYFIK
MCDELNVSYDVFIRTTEERHHASTQELWRRMEANGDLYLDRYEGWYSVRDEAFYDESELVAGEGGEKLSPQGTPVDWTVE
ESWFFRLSKYAEPLLKLYEENPGFIQPDSRRNEVMRFVEGGLRDLSVSRTSFDWGVKVPGCDGHVMYVWVDALTNYITGL
GFPDENGDFAKYWPANLHLIGKDIVRFHTVYWPAFLMSADIALPRQVFGHGFLLNRGQKESKSLGNVTDPLDLADRFGVD
PLRYFLMREVAFGQDGSYSAEAIVTRCNAELANSYGNLVQRTLSMIFKNMGGNLETFHSNVGDDELLATVFNACREELPR
EFSALNFSAGIEAWMRAVFACNAYVDEQAPWALRKTDPERMKAVLLTLFIAIRDLTVAISPVVPAAAAKVLDQLGIPREA
RGFDALTDADWYMARVATGERLAQPMPAFPRLELPEEEGSA

Sequences:

>Translated_521_residues
MGEPFYITTAISYPNGKPHIGHAYEAIAADVIARFQRAMGRDVRFQTGTDEHGLKMAQKARELGITPRELSDEMSSYFIK
MCDELNVSYDVFIRTTEERHHASTQELWRRMEANGDLYLDRYEGWYSVRDEAFYDESELVAGEGGEKLSPQGTPVDWTVE
ESWFFRLSKYAEPLLKLYEENPGFIQPDSRRNEVMRFVEGGLRDLSVSRTSFDWGVKVPGCDGHVMYVWVDALTNYITGL
GFPDENGDFAKYWPANLHLIGKDIVRFHTVYWPAFLMSADIALPRQVFGHGFLLNRGQKESKSLGNVTDPLDLADRFGVD
PLRYFLMREVAFGQDGSYSAEAIVTRCNAELANSYGNLVQRTLSMIFKNMGGNLETFHSNVGDDELLATVFNACREELPR
EFSALNFSAGIEAWMRAVFACNAYVDEQAPWALRKTDPERMKAVLLTLFIAIRDLTVAISPVVPAAAAKVLDQLGIPREA
RGFDALTDADWYMARVATGERLAQPMPAFPRLELPEEEGSA
>Mature_520_residues
GEPFYITTAISYPNGKPHIGHAYEAIAADVIARFQRAMGRDVRFQTGTDEHGLKMAQKARELGITPRELSDEMSSYFIKM
CDELNVSYDVFIRTTEERHHASTQELWRRMEANGDLYLDRYEGWYSVRDEAFYDESELVAGEGGEKLSPQGTPVDWTVEE
SWFFRLSKYAEPLLKLYEENPGFIQPDSRRNEVMRFVEGGLRDLSVSRTSFDWGVKVPGCDGHVMYVWVDALTNYITGLG
FPDENGDFAKYWPANLHLIGKDIVRFHTVYWPAFLMSADIALPRQVFGHGFLLNRGQKESKSLGNVTDPLDLADRFGVDP
LRYFLMREVAFGQDGSYSAEAIVTRCNAELANSYGNLVQRTLSMIFKNMGGNLETFHSNVGDDELLATVFNACREELPRE
FSALNFSAGIEAWMRAVFACNAYVDEQAPWALRKTDPERMKAVLLTLFIAIRDLTVAISPVVPAAAAKVLDQLGIPREAR
GFDALTDADWYMARVATGERLAQPMPAFPRLELPEEEGSA

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=508, Percent_Identity=37.007874015748, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI14043022, Length=557, Percent_Identity=26.3913824057451, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1788432, Length=506, Percent_Identity=29.4466403162055, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI212645096, Length=492, Percent_Identity=34.1463414634146, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71992781, Length=386, Percent_Identity=32.3834196891192, Blast_Score=211, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17540794, Length=558, Percent_Identity=25.4480286738351, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321610, Length=561, Percent_Identity=30.4812834224599, Blast_Score=274, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6321704, Length=548, Percent_Identity=23.7226277372263, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21358483, Length=503, Percent_Identity=34.3936381709742, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24655311, Length=552, Percent_Identity=24.8188405797101, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355409, Length=181, Percent_Identity=27.6243093922652, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58818; Mature: 58687

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEPFYITTAISYPNGKPHIGHAYEAIAADVIARFQRAMGRDVRFQTGTDEHGLKMAQKA
CCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHH
RELGITPRELSDEMSSYFIKMCDELNVSYDVFIRTTEERHHASTQELWRRMEANGDLYLD
HHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCHHHHHHHHHCCCCEEEE
RYEGWYSVRDEAFYDESELVAGEGGEKLSPQGTPVDWTVEESWFFRLSKYAEPLLKLYEE
CCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHC
NPGFIQPDSRRNEVMRFVEGGLRDLSVSRTSFDWGVKVPGCDGHVMYVWVDALTNYITGL
CCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCC
GFPDENGDFAKYWPANLHLIGKDIVRFHTVYWPAFLMSADIALPRQVFGHGFLLNRGQKE
CCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEECCCCHH
SKSLGNVTDPLDLADRFGVDPLRYFLMREVAFGQDGSYSAEAIVTRCNAELANSYGNLVQ
HHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RTLSMIFKNMGGNLETFHSNVGDDELLATVFNACREELPREFSALNFSAGIEAWMRAVFA
HHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHH
CNAYVDEQAPWALRKTDPERMKAVLLTLFIAIRDLTVAISPVVPAAAAKVLDQLGIPREA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
RGFDALTDADWYMARVATGERLAQPMPAFPRLELPEEEGSA
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
GEPFYITTAISYPNGKPHIGHAYEAIAADVIARFQRAMGRDVRFQTGTDEHGLKMAQKA
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHH
RELGITPRELSDEMSSYFIKMCDELNVSYDVFIRTTEERHHASTQELWRRMEANGDLYLD
HHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHCCCHHHHHHHHHCCCCEEEE
RYEGWYSVRDEAFYDESELVAGEGGEKLSPQGTPVDWTVEESWFFRLSKYAEPLLKLYEE
CCCCCCCCCHHHHCCCCCEEECCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHC
NPGFIQPDSRRNEVMRFVEGGLRDLSVSRTSFDWGVKVPGCDGHVMYVWVDALTNYITGL
CCCCCCCCCHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHCC
GFPDENGDFAKYWPANLHLIGKDIVRFHTVYWPAFLMSADIALPRQVFGHGFLLNRGQKE
CCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEECCCCHH
SKSLGNVTDPLDLADRFGVDPLRYFLMREVAFGQDGSYSAEAIVTRCNAELANSYGNLVQ
HHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
RTLSMIFKNMGGNLETFHSNVGDDELLATVFNACREELPREFSALNFSAGIEAWMRAVFA
HHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHH
CNAYVDEQAPWALRKTDPERMKAVLLTLFIAIRDLTVAISPVVPAAAAKVLDQLGIPREA
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
RGFDALTDADWYMARVATGERLAQPMPAFPRLELPEEEGSA
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]