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The map label for this gene is ispDF

Identifier: 87199942

GI number: 87199942

Start: 2036643

End: 2037803

Strand: Reverse

Name: ispDF

Synonym: Saro_1925

Alternate gene names: 87199942

Gene position: 2037803-2036643 (Counterclockwise)

Preceding gene: 87199950

Following gene: 87199941

Centisome position: 57.22

GC content: 64.6

Gene sequence:

>1161_bases
ATGAACAGTGTCCCGTCCCTTCCCGGCCAGTCGGTTGCCGCAGTTGTCGTTGCCGGTGGCAAGGGGCTGCGCACGGGCGG
GCCCGTGCCAAAGCAGTTCGTGATCTGGCGCGGAAAGCCGCTTTTGCGCCATTGCGTGGAGGCACTCGAGGCCGCCGGAA
TTGCACCGATCGTCGTCGCCATTCCCGCAGGCTGGGACGAAGCGGCGACGCAGGCGTTGGCGGGAATCTCCATGGTCCGC
CTCGTTCACGGCGGTGCGACACGGCAGGAATCTGTGAAGGCCGCGCTCGAAGTGCTGGAAGGCGATGCGCCCGCTCGCGT
GCTCATCCATGATGCCGCACGGCCAGACCTGCCGGGTTCCGTGATCGAAAGGCTCCTCACCGCGCTGGACAAGCGTACCG
GGGCCATTCCGGTGCTGCCCGTTGTGGACAGCATGGTGCGCGGATCCGGAGACGCGATGGGCGAAACGGTTGCCCGTGAA
GACCTGTATCGCGTCCAGACTCCGCAAGCGTTCCACTATCCGGCAATCCTTGCCGCCCATAGGGCCTGGCAGGGTGAGGC
TCTTGCCGGCGATGACGCGCAAGTGGCCATGCGAGCAGCGCACGAGATCGCGCTCGTCGAGGGCGATGAAGCATTGCGAA
AGGTGACGTTCGCGTCCGATCTCGAGGAGCAGAGCATGAGCGTCATTCCCCGCACCGGAATGGGCTTCGACGTCCATAGG
CTGGTGGAAGGCGAGGAACTTTGGCTTTGCGGGGTGAATATCCCGCACGGAAAAGGTCTTTCAGGACATTCGGATGCGGA
CGTCGCGATCCACGCACTTGTCGACGCATTGCTCGGCGCGATTGCGGCGGGGGATATCGGCGATCATTTTCCGCCGTCCG
ATCCGCAGTGGAAGGGGGCCTCGTCGGACCGTTTTCTCGCGCACGCGGGCACCCTGGTGACCGAAGCGGGTTACCGGATA
GCGAACGTCGACGTGACGATTATCTGCGAAGCCCCGAAGATCGGACCGCACAAGGCGGCCATGCGCGAGACGCTTGCCCG
GATTCTCGGGATTGACTCCGCGCTGGTCTCGGTCAAGGCGACGACGACGGAACGCCTTGGACTGACTGGTCGGGGCGAAG
GCATAGCGGCGCAGGCCGTGGCAACAGTTGTCTCGGGCTGA

Upstream 100 bases:

>100_bases
GTCAGCAATCTGCCTAAAAAACAGGCAGCGCTTAGTCGATTGTGGCCGGAAGGGCAAGGCTGTTGCCCGCGCGGGGCAAG
CGCTGGTAGAGGCGCGGCCG

Downstream 100 bases:

>100_bases
GCCTGCAGGAGCTTGTAATATGAGGCTTTCCCGTAATCTTCTCGCGCTGGGCGCGGGCCTTGCGATCGCCCAGGCCCAAG
TGGCCCAGGCTGCCGCCGCT

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MNSVPSLPGQSVAAVVVAGGKGLRTGGPVPKQFVIWRGKPLLRHCVEALEAAGIAPIVVAIPAGWDEAATQALAGISMVR
LVHGGATRQESVKAALEVLEGDAPARVLIHDAARPDLPGSVIERLLTALDKRTGAIPVLPVVDSMVRGSGDAMGETVARE
DLYRVQTPQAFHYPAILAAHRAWQGEALAGDDAQVAMRAAHEIALVEGDEALRKVTFASDLEEQSMSVIPRTGMGFDVHR
LVEGEELWLCGVNIPHGKGLSGHSDADVAIHALVDALLGAIAAGDIGDHFPPSDPQWKGASSDRFLAHAGTLVTEAGYRI
ANVDVTIICEAPKIGPHKAAMRETLARILGIDSALVSVKATTTERLGLTGRGEGIAAQAVATVVSG

Sequences:

>Translated_386_residues
MNSVPSLPGQSVAAVVVAGGKGLRTGGPVPKQFVIWRGKPLLRHCVEALEAAGIAPIVVAIPAGWDEAATQALAGISMVR
LVHGGATRQESVKAALEVLEGDAPARVLIHDAARPDLPGSVIERLLTALDKRTGAIPVLPVVDSMVRGSGDAMGETVARE
DLYRVQTPQAFHYPAILAAHRAWQGEALAGDDAQVAMRAAHEIALVEGDEALRKVTFASDLEEQSMSVIPRTGMGFDVHR
LVEGEELWLCGVNIPHGKGLSGHSDADVAIHALVDALLGAIAAGDIGDHFPPSDPQWKGASSDRFLAHAGTLVTEAGYRI
ANVDVTIICEAPKIGPHKAAMRETLARILGIDSALVSVKATTTERLGLTGRGEGIAAQAVATVVSG
>Mature_386_residues
MNSVPSLPGQSVAAVVVAGGKGLRTGGPVPKQFVIWRGKPLLRHCVEALEAAGIAPIVVAIPAGWDEAATQALAGISMVR
LVHGGATRQESVKAALEVLEGDAPARVLIHDAARPDLPGSVIERLLTALDKRTGAIPVLPVVDSMVRGSGDAMGETVARE
DLYRVQTPQAFHYPAILAAHRAWQGEALAGDDAQVAMRAAHEIALVEGDEALRKVTFASDLEEQSMSVIPRTGMGFDVHR
LVEGEELWLCGVNIPHGKGLSGHSDADVAIHALVDALLGAIAAGDIGDHFPPSDPQWKGASSDRFLAHAGTLVTEAGYRI
ANVDVTIICEAPKIGPHKAAMRETLARILGIDSALVSVKATTTERLGLTGRGEGIAAQAVATVVSG

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=226, Percent_Identity=32.7433628318584, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=54.1935483870968, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789104, Length=217, Percent_Identity=37.3271889400922, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_NOVAD (Q2G708)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497199.1
- ProteinModelPortal:   Q2G708
- SMR:   Q2G708
- STRING:   Q2G708
- GeneID:   3917148
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1925
- NMPDR:   fig|48935.1.peg.896
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q2G708
- ProtClustDB:   PRK09382
- BioCyc:   NARO279238:SARO_1925-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 40153; Mature: 40153

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVPSLPGQSVAAVVVAGGKGLRTGGPVPKQFVIWRGKPLLRHCVEALEAAGIAPIVVA
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
IPAGWDEAATQALAGISMVRLVHGGATRQESVKAALEVLEGDAPARVLIHDAARPDLPGS
ECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHH
VIERLLTALDKRTGAIPVLPVVDSMVRGSGDAMGETVAREDLYRVQTPQAFHYPAILAAH
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
RAWQGEALAGDDAQVAMRAAHEIALVEGDEALRKVTFASDLEEQSMSVIPRTGMGFDVHR
HCCCCCCCCCCHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LVEGEELWLCGVNIPHGKGLSGHSDADVAIHALVDALLGAIAAGDIGDHFPPSDPQWKGA
HCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SSDRFLAHAGTLVTEAGYRIANVDVTIICEAPKIGPHKAAMRETLARILGIDSALVSVKA
CCCCCHHHHCHHHHHCCCEEEECEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHEEEEE
TTTERLGLTGRGEGIAAQAVATVVSG
CCHHHCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNSVPSLPGQSVAAVVVAGGKGLRTGGPVPKQFVIWRGKPLLRHCVEALEAAGIAPIVVA
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
IPAGWDEAATQALAGISMVRLVHGGATRQESVKAALEVLEGDAPARVLIHDAARPDLPGS
ECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHH
VIERLLTALDKRTGAIPVLPVVDSMVRGSGDAMGETVAREDLYRVQTPQAFHYPAILAAH
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
RAWQGEALAGDDAQVAMRAAHEIALVEGDEALRKVTFASDLEEQSMSVIPRTGMGFDVHR
HCCCCCCCCCCHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LVEGEELWLCGVNIPHGKGLSGHSDADVAIHALVDALLGAIAAGDIGDHFPPSDPQWKGA
HCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
SSDRFLAHAGTLVTEAGYRIANVDVTIICEAPKIGPHKAAMRETLARILGIDSALVSVKA
CCCCCHHHHCHHHHHCCCEEEECEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHEEEEE
TTTERLGLTGRGEGIAAQAVATVVSG
CCHHHCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA