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The map label for this gene is mazG [H]

Identifier: 87199950

GI number: 87199950

Start: 2047316

End: 2048140

Strand: Reverse

Name: mazG [H]

Synonym: Saro_1933

Alternate gene names: 87199950

Gene position: 2048140-2047316 (Counterclockwise)

Preceding gene: 87199954

Following gene: 87199942

Centisome position: 57.51

GC content: 61.7

Gene sequence:

>825_bases
ATGGCCCCTTCTGAAAGTCCTGCCCTCGAAAGCAGCGGCTCCGAGATGAACACGAACCTCTCGCGGCTGCTCTCAATCAT
GGCCAGGCTGCGCGATCCGGAGCGTGGTTGCGAATGGGACTGCGCCCAAACCTTTGCGACGATCGCACCTTACACCATCG
AGGAAGCCTACGAAGTCGACGATGCGATCTCGCGCGGCGACATGGCGGCGCTCAAGGAGGAACTCGGCGACTTGCTGCTC
CAGGTCGTATTTCACGCTCGGATTGCCGAGGAGCTCGGCCTCTTCGGGTTCGACGATGTGGCTGCTGCCATCGCGGACAA
GATGGAAGCCCGCCATCCGCACGTGTTCGGCAATGAGGTAGATGAAGGTCAATCCCGCGAGGACCGCTGGGAACGACTCA
AAGCGGCCGAGCGGCAATCGAAAGGCGCAAAAGGTGCGCTTGATGGCGTCGCACTCGCTCTTCCAGCTCTTATCCGGGCC
GAAAAACTTCAGAAGCGTGCAGCGCGGCAGGGCTTCGACTGGCCCGATGCCGCCGGCGCAGCGGAGAAGGTTGTCGAGGA
AATCCAGGAACTTGACGAGGCTTCTTCGGAATCGGAGCGCATATTGGAAGCTGGCGATCTACTTTTTGCCGCCGTCAACG
TCGTTCGCAAGTACGGCGTGGCGCCGGAAGATGCACTGAGAGCGGCGAATGCCAAGTTCGAGCGACGCTACGGCGCCATG
GAGGCCCTGGCTGATGGCCGATTCGCCACGCTGTCGCTCGATGAACAGGAAGCGCTCTGGCAGGCTGTGAAGCGAGCCGA
GGCGGCGCAGGTCACAGGCTGGTAA

Upstream 100 bases:

>100_bases
CAAGCCTTTCGCCATCACACTGGACACAGTTTACATGGACAGTTATGGTATCGGTATTTACATAATGATTATTATCAATC
CTGAGTGTGAGGCACGCGCA

Downstream 100 bases:

>100_bases
AGCGCCCCAGTTCCCGTGGCGTGAGGCGCACCTTTATGCGCATCGCGGGTCCAATTTCTGCAATATCCACTTGATTTTCC
GAAATTATGTCGCCGTGTGC

Product: MazG family protein

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MAPSESPALESSGSEMNTNLSRLLSIMARLRDPERGCEWDCAQTFATIAPYTIEEAYEVDDAISRGDMAALKEELGDLLL
QVVFHARIAEELGLFGFDDVAAAIADKMEARHPHVFGNEVDEGQSREDRWERLKAAERQSKGAKGALDGVALALPALIRA
EKLQKRAARQGFDWPDAAGAAEKVVEEIQELDEASSESERILEAGDLLFAAVNVVRKYGVAPEDALRAANAKFERRYGAM
EALADGRFATLSLDEQEALWQAVKRAEAAQVTGW

Sequences:

>Translated_274_residues
MAPSESPALESSGSEMNTNLSRLLSIMARLRDPERGCEWDCAQTFATIAPYTIEEAYEVDDAISRGDMAALKEELGDLLL
QVVFHARIAEELGLFGFDDVAAAIADKMEARHPHVFGNEVDEGQSREDRWERLKAAERQSKGAKGALDGVALALPALIRA
EKLQKRAARQGFDWPDAAGAAEKVVEEIQELDEASSESERILEAGDLLFAAVNVVRKYGVAPEDALRAANAKFERRYGAM
EALADGRFATLSLDEQEALWQAVKRAEAAQVTGW
>Mature_273_residues
APSESPALESSGSEMNTNLSRLLSIMARLRDPERGCEWDCAQTFATIAPYTIEEAYEVDDAISRGDMAALKEELGDLLLQ
VVFHARIAEELGLFGFDDVAAAIADKMEARHPHVFGNEVDEGQSREDRWERLKAAERQSKGAKGALDGVALALPALIRAE
KLQKRAARQGFDWPDAAGAAEKVVEEIQELDEASSESERILEAGDLLFAAVNVVRKYGVAPEDALRAANAKFERRYGAME
ALADGRFATLSLDEQEALWQAVKRAEAAQVTGW

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=265, Percent_Identity=50.5660377358491, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 29994; Mature: 29863

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPSESPALESSGSEMNTNLSRLLSIMARLRDPERGCEWDCAQTFATIAPYTIEEAYEVD
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
DAISRGDMAALKEELGDLLLQVVFHARIAEELGLFGFDDVAAAIADKMEARHPHVFGNEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
DEGQSREDRWERLKAAERQSKGAKGALDGVALALPALIRAEKLQKRAARQGFDWPDAAGA
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
AEKVVEEIQELDEASSESERILEAGDLLFAAVNVVRKYGVAPEDALRAANAKFERRYGAM
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EALADGRFATLSLDEQEALWQAVKRAEAAQVTGW
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
APSESPALESSGSEMNTNLSRLLSIMARLRDPERGCEWDCAQTFATIAPYTIEEAYEVD
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHH
DAISRGDMAALKEELGDLLLQVVFHARIAEELGLFGFDDVAAAIADKMEARHPHVFGNEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
DEGQSREDRWERLKAAERQSKGAKGALDGVALALPALIRAEKLQKRAARQGFDWPDAAGA
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
AEKVVEEIQELDEASSESERILEAGDLLFAAVNVVRKYGVAPEDALRAANAKFERRYGAM
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EALADGRFATLSLDEQEALWQAVKRAEAAQVTGW
HHHHCCCEEEECCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]