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The map label for this gene is lcfB [H]

Identifier: 87199549

GI number: 87199549

Start: 1574831

End: 1576426

Strand: Reverse

Name: lcfB [H]

Synonym: Saro_1531

Alternate gene names: 87199549

Gene position: 1576426-1574831 (Counterclockwise)

Preceding gene: 87199550

Following gene: 87199548

Centisome position: 44.26

GC content: 63.66

Gene sequence:

>1596_bases
ATGGACTGGAACTTTGGAGACCTACTGGATGCGACCGCCGCAAATGTGCCCGGCGATCGCCCCGCCATCATCCGCGGAGA
CCGGGTCGTCGAATGGGGGGATTTCGACGCGCGGACGAACCGGCTGGCGCGCGCCATGCTGGCAGCCGGCCTGCCTACCG
GCGCCCGCGTCGCCATTCTCGCACGCAACATTCCGGAATTCATCGAGATTGCCGCTGCTGCGTTCAAGGCGCGACTGGCG
CACGTCAACCTCAATTACCGCTACACAACATCCGAAATCGAATACGTCCTGCGCGACTGTCAGGCCGCAGCAATCTTTTA
CCAGGACGAATTCGCACCGGTTGTGGCCCCGCTGATCAATGGGTCCATCGATCACCTTAGCTATGCAGTACAGATCGGCG
ATGGCGGTGCCTATGACGACATCGTGACCATCGGCGACCCCGAGCCTCTCGGGATCGAGCGCTCCCCTGACGACGGCTAT
CTGCTCTATACGGGTGGCACGACCGGACGGCCGAAGGGGGTGATGTGGCGCGCTGCAGACGCGCGGCAGACGCAATTGGA
ATCTCCGGTCGCAGTGACTTCCCCTGCCAGCATGGATGACCATGTGGCGCAGGTCTTGGCGGGCCGCGCTGGGCGCGTCA
TGCCTGCCTGCCCGCTCATGCACGGGGCCGGGCTGACCAGTTCCCTGGCAGAACTGCTCATTGGCGGGACCGTAGTGCTT
CTGCCATCGAACAGATTCAGTGCGCTGGAGTTGTGGGAGGAGGCCTCGCGCAATCGCGTCACGCGCATCCTGATCGTCGG
AGACGCATTTGCACGCCCCATGGCCGATACGCTGGCTGCCAATCGGGAACGTTGGAACCTGGATTCGCTGCGCCTGATCT
CGTCGGGCGGCCTTATGTGGTCGCAGCAGGTCAAGGAAGCGCTGCTCGAGGCCCTGCCCGGCATCACATTGCTGGACACG
TTGGGCGCCTCCGAGGCTTCGGGCTTCGGTTATGCAATCACGACAAAGGATCGCGCCACGCCCACCGGGCTGTTCGAACC
CGCACCGCATACGGTGCTTATCGACCCTGACAGGGATCGAGTGCTCGGAAGCGATGAAGCAGGCAAGGGTTGGCTGGCCC
GACGTCCGCCCTTCGCCAGCGGATATCATGGCGATCCTGTGAAAACCGCCAGCGTGTATCGCATCATCGACGGCGAGCGC
CTGGCCGTTCCCGGCGACATGGCAGAACGCTTGCCCGATGGGCGACTTCGCCTCCTCGGTCGCGACAGCATGTGCGTCAA
CACGGGAGGCGAGAAGGTTTTCGTTGAAGAGGTCGAAGAGGCGCTGAAGCGCGTAAACGGCGTCGCCGATGCCATGGTGT
TCGGCCTGCCCGACCCAAAATGGGGAAGCGTGGTGACAGCGCTGATCGAGGCCGGCCCCTCTCCCGATGACGAGGTCATC
CGCACCGCCCTGTCGCACGATCTTGCCGCTTACAAGCAGCCGCGCACGATCATTCGTGTCGCAGTGCTGCCGCGACACGC
GAATGGCAAGAGCGACTACCGCACCGGCATGGAGATCGCACAACGACAGATCGCCACCGGGCCGTCTGCCGACTGA

Upstream 100 bases:

>100_bases
AATACCGCCAAGCTGGCGTCGTCGCACGATGCCGGGGAAGCCGTCGCCGCTTTCGTCGAAAAGCGGCCGCCCGTGTTTCT
CGGTCGCTGAGGGTAAGTCC

Downstream 100 bases:

>100_bases
GTCGCACCCCGCAACCTTACCCGCGCGTCGCTGCACTAGCCGGACGCTTTCCAGCGCCACGGGATCAGTTCCCGAACTAC
CCCAAGGATGTTCTATGCAA

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MDWNFGDLLDATAANVPGDRPAIIRGDRVVEWGDFDARTNRLARAMLAAGLPTGARVAILARNIPEFIEIAAAAFKARLA
HVNLNYRYTTSEIEYVLRDCQAAAIFYQDEFAPVVAPLINGSIDHLSYAVQIGDGGAYDDIVTIGDPEPLGIERSPDDGY
LLYTGGTTGRPKGVMWRAADARQTQLESPVAVTSPASMDDHVAQVLAGRAGRVMPACPLMHGAGLTSSLAELLIGGTVVL
LPSNRFSALELWEEASRNRVTRILIVGDAFARPMADTLAANRERWNLDSLRLISSGGLMWSQQVKEALLEALPGITLLDT
LGASEASGFGYAITTKDRATPTGLFEPAPHTVLIDPDRDRVLGSDEAGKGWLARRPPFASGYHGDPVKTASVYRIIDGER
LAVPGDMAERLPDGRLRLLGRDSMCVNTGGEKVFVEEVEEALKRVNGVADAMVFGLPDPKWGSVVTALIEAGPSPDDEVI
RTALSHDLAAYKQPRTIIRVAVLPRHANGKSDYRTGMEIAQRQIATGPSAD

Sequences:

>Translated_531_residues
MDWNFGDLLDATAANVPGDRPAIIRGDRVVEWGDFDARTNRLARAMLAAGLPTGARVAILARNIPEFIEIAAAAFKARLA
HVNLNYRYTTSEIEYVLRDCQAAAIFYQDEFAPVVAPLINGSIDHLSYAVQIGDGGAYDDIVTIGDPEPLGIERSPDDGY
LLYTGGTTGRPKGVMWRAADARQTQLESPVAVTSPASMDDHVAQVLAGRAGRVMPACPLMHGAGLTSSLAELLIGGTVVL
LPSNRFSALELWEEASRNRVTRILIVGDAFARPMADTLAANRERWNLDSLRLISSGGLMWSQQVKEALLEALPGITLLDT
LGASEASGFGYAITTKDRATPTGLFEPAPHTVLIDPDRDRVLGSDEAGKGWLARRPPFASGYHGDPVKTASVYRIIDGER
LAVPGDMAERLPDGRLRLLGRDSMCVNTGGEKVFVEEVEEALKRVNGVADAMVFGLPDPKWGSVVTALIEAGPSPDDEVI
RTALSHDLAAYKQPRTIIRVAVLPRHANGKSDYRTGMEIAQRQIATGPSAD
>Mature_531_residues
MDWNFGDLLDATAANVPGDRPAIIRGDRVVEWGDFDARTNRLARAMLAAGLPTGARVAILARNIPEFIEIAAAAFKARLA
HVNLNYRYTTSEIEYVLRDCQAAAIFYQDEFAPVVAPLINGSIDHLSYAVQIGDGGAYDDIVTIGDPEPLGIERSPDDGY
LLYTGGTTGRPKGVMWRAADARQTQLESPVAVTSPASMDDHVAQVLAGRAGRVMPACPLMHGAGLTSSLAELLIGGTVVL
LPSNRFSALELWEEASRNRVTRILIVGDAFARPMADTLAANRERWNLDSLRLISSGGLMWSQQVKEALLEALPGITLLDT
LGASEASGFGYAITTKDRATPTGLFEPAPHTVLIDPDRDRVLGSDEAGKGWLARRPPFASGYHGDPVKTASVYRIIDGER
LAVPGDMAERLPDGRLRLLGRDSMCVNTGGEKVFVEEVEEALKRVNGVADAMVFGLPDPKWGSVVTALIEAGPSPDDEVI
RTALSHDLAAYKQPRTIIRVAVLPRHANGKSDYRTGMEIAQRQIATGPSAD

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=551, Percent_Identity=25.5898366606171, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI187761343, Length=551, Percent_Identity=25.5898366606171, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI38505220, Length=487, Percent_Identity=24.0246406570842, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788107, Length=540, Percent_Identity=25, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI145693145, Length=551, Percent_Identity=25.0453720508167, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=25.5597014925373, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI221142682, Length=516, Percent_Identity=23.2558139534884, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=535, Percent_Identity=21.3084112149533, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 57125; Mature: 57125

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWNFGDLLDATAANVPGDRPAIIRGDRVVEWGDFDARTNRLARAMLAAGLPTGARVAIL
CCCCHHHHHHHHHCCCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEE
ARNIPEFIEIAAAAFKARLAHVNLNYRYTTSEIEYVLRDCQAAAIFYQDEFAPVVAPLIN
ECCCHHHHHHHHHHHHHEEEEEEEEEEEEHHHHHHHHHHHHHEEEEEECCCHHHHHHHHC
GSIDHLSYAVQIGDGGAYDDIVTIGDPEPLGIERSPDDGYLLYTGGTTGRPKGVMWRAAD
CCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCC
ARQTQLESPVAVTSPASMDDHVAQVLAGRAGRVMPACPLMHGAGLTSSLAELLIGGTVVL
CHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEE
LPSNRFSALELWEEASRNRVTRILIVGDAFARPMADTLAANRERWNLDSLRLISSGGLMW
EECCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCH
SQQVKEALLEALPGITLLDTLGASEASGFGYAITTKDRATPTGLFEPAPHTVLIDPDRDR
HHHHHHHHHHHCCCCEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCC
VLGSDEAGKGWLARRPPFASGYHGDPVKTASVYRIIDGERLAVPGDMAERLPDGRLRLLG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCEEEEEE
RDSMCVNTGGEKVFVEEVEEALKRVNGVADAMVFGLPDPKWGSVVTALIEAGPSPDDEVI
CCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCHHHHH
RTALSHDLAAYKQPRTIIRVAVLPRHANGKSDYRTGMEIAQRQIATGPSAD
HHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDWNFGDLLDATAANVPGDRPAIIRGDRVVEWGDFDARTNRLARAMLAAGLPTGARVAIL
CCCCHHHHHHHHHCCCCCCCCEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEE
ARNIPEFIEIAAAAFKARLAHVNLNYRYTTSEIEYVLRDCQAAAIFYQDEFAPVVAPLIN
ECCCHHHHHHHHHHHHHEEEEEEEEEEEEHHHHHHHHHHHHHEEEEEECCCHHHHHHHHC
GSIDHLSYAVQIGDGGAYDDIVTIGDPEPLGIERSPDDGYLLYTGGTTGRPKGVMWRAAD
CCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCC
ARQTQLESPVAVTSPASMDDHVAQVLAGRAGRVMPACPLMHGAGLTSSLAELLIGGTVVL
CHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEE
LPSNRFSALELWEEASRNRVTRILIVGDAFARPMADTLAANRERWNLDSLRLISSGGLMW
EECCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCH
SQQVKEALLEALPGITLLDTLGASEASGFGYAITTKDRATPTGLFEPAPHTVLIDPDRDR
HHHHHHHHHHHCCCCEEEHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCC
VLGSDEAGKGWLARRPPFASGYHGDPVKTASVYRIIDGERLAVPGDMAERLPDGRLRLLG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCEEEEEE
RDSMCVNTGGEKVFVEEVEEALKRVNGVADAMVFGLPDPKWGSVVTALIEAGPSPDDEVI
CCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHCCCCCHHHHH
RTALSHDLAAYKQPRTIIRVAVLPRHANGKSDYRTGMEIAQRQIATGPSAD
HHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]