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The map label for this gene is paaF [H]

Identifier: 87199550

GI number: 87199550

Start: 1576437

End: 1577201

Strand: Reverse

Name: paaF [H]

Synonym: Saro_1532

Alternate gene names: 87199550

Gene position: 1577201-1576437 (Counterclockwise)

Preceding gene: 87199552

Following gene: 87199549

Centisome position: 44.28

GC content: 59.35

Gene sequence:

>765_bases
ATGAACAGCATTCTAACCGACGTTAAAGACAACGTGGCCGTCGTTACAATGGCCAACCCACCTGTCAACGCCATTGCCAT
GGAAATGCTGCAAGAACTGACTGAAGTCTTTGACAGCTTCAACGATCAGGAAGACGTGCGCGTCGCGGTCCTGACTGGCG
CAGGAAAATGCTTCAGCGCTGGAGCCGACCTCAAAGGGCGACCTGACCTTTCGGTGCCTGGAGCCCGCTGGAGCCGCAAT
CGTATTGTGCGCGAAGTCGCCTACGCGATTGCCGATTGTGCGAAGCCCGTCATCGCCGCTGTGAACGGCCCAGCACTCGG
AGCCGGATTCGGCCTGGTGGCGAGCTGCGATATCATCGTCGCGTCTGAAAATGCCGTGTTCGGCCTCCCGGAAATCGACG
TAGGACTAATGGGTGGAGGCAAGCACTCGTCACGTATCGTGCCTCATTCATTGACGCGCCGCATGATGCTGACTGGCTAT
CGGGTGCCTGCATCCGAAATGTATCGCCGAGGCATCATCGAGGCATGTCTGCCTGCCGACGAGCTGCTGCCCTATTGCAT
GGACATGGCTGCAGTGATCGCATCCAAGAGCCCGCTCGCGACGCGCTATGCCAAGGACAGCATGCGCACCATCGAGAACA
TGACGCTGCGCGACGGCTACATTTACGAGCAGGGAAATACCGCCAAGCTGGCGTCGTCGCACGATGCCGGGGAAGCCGTC
GCCGCTTTCGTCGAAAAGCGGCCGCCCGTGTTTCTCGGTCGCTGA

Upstream 100 bases:

>100_bases
AAGGCGTCAATCCAGAGTGCGATTTACCGACGTGGCAGGCGCTTCCACTTCTAAACAACACACATCTTTTGCCATCTGCC
TCCAAGAATTGGAGAGGCAC

Downstream 100 bases:

>100_bases
GGGTAAGTCCATGGACTGGAACTTTGGAGACCTACTGGATGCGACCGCCGCAAATGTGCCCGGCGATCGCCCCGCCATCA
TCCGCGGAGACCGGGTCGTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNSILTDVKDNVAVVTMANPPVNAIAMEMLQELTEVFDSFNDQEDVRVAVLTGAGKCFSAGADLKGRPDLSVPGARWSRN
RIVREVAYAIADCAKPVIAAVNGPALGAGFGLVASCDIIVASENAVFGLPEIDVGLMGGGKHSSRIVPHSLTRRMMLTGY
RVPASEMYRRGIIEACLPADELLPYCMDMAAVIASKSPLATRYAKDSMRTIENMTLRDGYIYEQGNTAKLASSHDAGEAV
AAFVEKRPPVFLGR

Sequences:

>Translated_254_residues
MNSILTDVKDNVAVVTMANPPVNAIAMEMLQELTEVFDSFNDQEDVRVAVLTGAGKCFSAGADLKGRPDLSVPGARWSRN
RIVREVAYAIADCAKPVIAAVNGPALGAGFGLVASCDIIVASENAVFGLPEIDVGLMGGGKHSSRIVPHSLTRRMMLTGY
RVPASEMYRRGIIEACLPADELLPYCMDMAAVIASKSPLATRYAKDSMRTIENMTLRDGYIYEQGNTAKLASSHDAGEAV
AAFVEKRPPVFLGR
>Mature_254_residues
MNSILTDVKDNVAVVTMANPPVNAIAMEMLQELTEVFDSFNDQEDVRVAVLTGAGKCFSAGADLKGRPDLSVPGARWSRN
RIVREVAYAIADCAKPVIAAVNGPALGAGFGLVASCDIIVASENAVFGLPEIDVGLMGGGKHSSRIVPHSLTRRMMLTGY
RVPASEMYRRGIIEACLPADELLPYCMDMAAVIASKSPLATRYAKDSMRTIENMTLRDGYIYEQGNTAKLASSHDAGEAV
AAFVEKRPPVFLGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=34.6774193548387, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI31542718, Length=265, Percent_Identity=30.5660377358491, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI70995211, Length=259, Percent_Identity=30.1158301158301, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI68989263, Length=218, Percent_Identity=28.4403669724771, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI37594469, Length=207, Percent_Identity=27.536231884058, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI37594471, Length=207, Percent_Identity=27.536231884058, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI20127408, Length=207, Percent_Identity=27.0531400966184, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4502327, Length=241, Percent_Identity=29.045643153527, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI45643119, Length=202, Percent_Identity=28.2178217821782, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260275230, Length=202, Percent_Identity=28.2178217821782, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260274832, Length=202, Percent_Identity=28.2178217821782, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI1787659, Length=271, Percent_Identity=33.5793357933579, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI221142681, Length=248, Percent_Identity=37.0967741935484, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI87082183, Length=242, Percent_Identity=26.8595041322314, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=29.1187739463602, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=28.1124497991968, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=26.4550264550265, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554946, Length=254, Percent_Identity=33.0708661417323, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=34.1176470588235, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17536985, Length=247, Percent_Identity=31.1740890688259, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17534483, Length=259, Percent_Identity=27.4131274131274, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=247, Percent_Identity=28.3400809716599, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=214, Percent_Identity=28.0373831775701, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=219, Percent_Identity=26.9406392694064, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17535521, Length=233, Percent_Identity=22.3175965665236, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=34.2741935483871, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=34.2741935483871, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19920382, Length=246, Percent_Identity=31.3008130081301, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922422, Length=250, Percent_Identity=28.4, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=27.1317829457364, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24654903, Length=185, Percent_Identity=30.8108108108108, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24583165, Length=225, Percent_Identity=24.4444444444444, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19921018, Length=251, Percent_Identity=24.7011952191235, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27182; Mature: 27182

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSILTDVKDNVAVVTMANPPVNAIAMEMLQELTEVFDSFNDQEDVRVAVLTGAGKCFSA
CCCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
GADLKGRPDLSVPGARWSRNRIVREVAYAIADCAKPVIAAVNGPALGAGFGLVASCDIIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
ASENAVFGLPEIDVGLMGGGKHSSRIVPHSLTRRMMLTGYRVPASEMYRRGIIEACLPAD
ECCCCEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
ELLPYCMDMAAVIASKSPLATRYAKDSMRTIENMTLRDGYIYEQGNTAKLASSHDAGEAV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEECCCEEEECCCCEEECCCCCHHHHH
AAFVEKRPPVFLGR
HHHHHCCCCEECCC
>Mature Secondary Structure
MNSILTDVKDNVAVVTMANPPVNAIAMEMLQELTEVFDSFNDQEDVRVAVLTGAGKCFSA
CCCCHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
GADLKGRPDLSVPGARWSRNRIVREVAYAIADCAKPVIAAVNGPALGAGFGLVASCDIIV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEE
ASENAVFGLPEIDVGLMGGGKHSSRIVPHSLTRRMMLTGYRVPASEMYRRGIIEACLPAD
ECCCCEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHH
ELLPYCMDMAAVIASKSPLATRYAKDSMRTIENMTLRDGYIYEQGNTAKLASSHDAGEAV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCEECCCEEEECCCCEEECCCCCHHHHH
AAFVEKRPPVFLGR
HHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]