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The map label for this gene is ycfF [C]

Identifier: 87199034

GI number: 87199034

Start: 1053510

End: 1053887

Strand: Direct

Name: ycfF [C]

Synonym: Saro_1012

Alternate gene names: 87199034

Gene position: 1053510-1053887 (Clockwise)

Preceding gene: 87199033

Following gene: 87199035

Centisome position: 29.58

GC content: 64.02

Gene sequence:

>378_bases
ATGGCCGTTGACGCTACCCTGCCTTATGACGACCAGAACATCTTTGCGAGGATCCTGCGCGGCGAGATCCCCAATCGCAC
GGTCTATGAAGACGATTTCGCGCTCGCGTTCCACGACATCAATCCTCAGGCGCCCACGCACGTCCTGGTGATCCCCAAGG
GCGCTTACGTTTGCTGGGACGATTTCTCGGCCAAGGCCTCCGACGCCGAGATCGCCGGCTTCATCCGGGCGGTCGGCAAG
GTGGCGCGCGATCTGGGCCTGGTTGCCCCGGGGTACCGCCTGCTCGCAAATGCCGGCACGGACAGCCACCAGGAAGTGCC
GCACCTTCACGTCCATCTTTTCGCCGGTCGTCCGCTCGGACCGATGCTGGTGCGGTGA

Upstream 100 bases:

>100_bases
TTGTCGGCCAAGGACGTGCCGTTCGCCGAAGTCCTGGCCGAACTCGCGCGCCGCGAAGGCACCTCGGGCATCGCCGAGAA
AGCCAGCCGGAGCAACTGAC

Downstream 100 bases:

>100_bases
AACGGGTCGAAGCGGGGGTTATCGGTTAACCGGCCCTTGCCCTTCGACTCGTCGCAAGGGTAGGGTCGCGGTCGAATGTC
CTTGCCCCAGCCGCCCTCCG

Product: histidine triad (HIT) protein

Products: ADP

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 125; Mature: 124

Protein sequence:

>125_residues
MAVDATLPYDDQNIFARILRGEIPNRTVYEDDFALAFHDINPQAPTHVLVIPKGAYVCWDDFSAKASDAEIAGFIRAVGK
VARDLGLVAPGYRLLANAGTDSHQEVPHLHVHLFAGRPLGPMLVR

Sequences:

>Translated_125_residues
MAVDATLPYDDQNIFARILRGEIPNRTVYEDDFALAFHDINPQAPTHVLVIPKGAYVCWDDFSAKASDAEIAGFIRAVGK
VARDLGLVAPGYRLLANAGTDSHQEVPHLHVHLFAGRPLGPMLVR
>Mature_124_residues
AVDATLPYDDQNIFARILRGEIPNRTVYEDDFALAFHDINPQAPTHVLVIPKGAYVCWDDFSAKASDAEIAGFIRAVGKV
ARDLGLVAPGYRLLANAGTDSHQEVPHLHVHLFAGRPLGPMLVR

Specific function: Unknown

COG id: COG0537

COG function: function code FGR; Diadenosine tetraphosphate (Ap4A) hydrolase and other HIT family hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HIT domain [H]

Homologues:

Organism=Homo sapiens, GI4885413, Length=108, Percent_Identity=47.2222222222222, Blast_Score=94, Evalue=3e-20,
Organism=Homo sapiens, GI14211923, Length=107, Percent_Identity=39.2523364485981, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1787346, Length=113, Percent_Identity=46.0176991150442, Blast_Score=100, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17506713, Length=110, Percent_Identity=46.3636363636364, Blast_Score=95, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28574010, Length=115, Percent_Identity=45.2173913043478, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24581222, Length=115, Percent_Identity=45.2173913043478, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011146
- InterPro:   IPR011151
- InterPro:   IPR019808
- InterPro:   IPR001310 [H]

Pfam domain/function: PF01230 HIT [H]

EC number: 3.6.1.41

Molecular weight: Translated: 13688; Mature: 13557

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00892 HIT_1 ; PS51084 HIT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVDATLPYDDQNIFARILRGEIPNRTVYEDDFALAFHDINPQAPTHVLVIPKGAYVCWD
CCCCCCCCCCCHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCEEEEEECCCCEEEEC
DFSAKASDAEIAGFIRAVGKVARDLGLVAPGYRLLANAGTDSHQEVPHLHVHLFAGRPLG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCC
PMLVR
CCCCC
>Mature Secondary Structure 
AVDATLPYDDQNIFARILRGEIPNRTVYEDDFALAFHDINPQAPTHVLVIPKGAYVCWD
CCCCCCCCCCHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCEEEEEECCCCEEEEC
DFSAKASDAEIAGFIRAVGKVARDLGLVAPGYRLLANAGTDSHQEVPHLHVHLFAGRPLG
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCC
PMLVR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: P1,P4-bis(5'-adenosyl) tetraphosphate; H2O

Specific reaction: P1,P4-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2664449; 1472710 [H]