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The map label for this gene is pyk [H]

Identifier: 87198457

GI number: 87198457

Start: 469798

End: 471252

Strand: Direct

Name: pyk [H]

Synonym: Saro_0432

Alternate gene names: 87198457

Gene position: 469798-471252 (Clockwise)

Preceding gene: 87198452

Following gene: 87198459

Centisome position: 13.19

GC content: 67.49

Gene sequence:

>1455_bases
GTGGCCAAGCTCGATCCTCGCGGACGCAAGGTAAAGATCCTCGCCACGGTCGGCCCTGCCAGCCGCGATCCGGAAATGCT
CGAAAAGCTGTTCCGCGCCGGCGCCGACGCCTTCCGCGTCAACATGAGCCACGGCGAACACGCGATTCACGCCGAGACCA
TCGCCAACATCCGCGCGCTGGAAAAGAAGGTGAACCGGCCGATCACGATCCTGTGCGACCTGCAGGGGCCCAAGCTTCGC
GTCGGCAAGTTCAAGGACGGCCGCGCGGTCATCCGCCATTCGGGCCACTTCACGCTCGATCGCAATCCCGAGCCGGGCGA
TGAAACCCGCGTCTGCCTGCCGCATCCCGAACTGTTCGGCGTTCTTCACAAGGGCCAGCGCCTGCTGATCGACGACGGCA
AGCTGCGGCTGCGGGTGATCCGCGCCGACGAGAACGAGATCCTCTGTTCGGCCGAAGTCGGCGGCGTCATTTCCGATCGC
AAGGGCGTCAACGTTCCCGATGCGGTGGTGCCCGTTCCCGCGCTGACCGACAAGGATCGGCGCGACCTGTCGTTCGCGGT
TGAGCAGGGGGCGGACTGGATCGCGCTGTCGTTCGTCCAGCGGCCCGAGGACGTGGCCGAGGCGCGGCGCCTGCTGGGCG
GCCACGGCTCGCTCATGGCCAAGATCGAAAAGCCGGCGGCGATTTCCCGGCTTGAAGAGATCGTCGAGCTTTCGGACGGC
ATCATGGTCGCCCGCGGCGACCTTGGCGTCGAGCTGAACCCTGAAGAGGTGCCGCCGCTCCAGAAGCGCATCGTCGAAAC
CGCGCGCCGCCAGGGCAAGCCGGTGGTCGTCGCCACGCAGATGCTCGAATCGATGATCGAAGCGCCGACGCCGACCCGCG
CCGAAGTTTCGGACGTCGCCAATGCGGTCTATGACGGCGCCGACGCGGTGATGCTCTCGGCCGAAACCGCCGCCGGTGCC
TGGCCGGAAGAGGCGGTCACCATCATGCACCGCATCGCCGCGCAGGTCGAAGCCGATCCGGGCTATGGCGCGCGCGTCCA
CTTCTTAGAGACGCTTCCCGATCCGACCACCGCCGACGCCCTTGCGCAGGCTGCGGCGAGCATCACCGAGACCTTGCCGA
TTTCCGGGATCATCGTGTTTACCGGATCGGGTTCCACCGCTCGCCGCGTGGCGCGCGAACGTCCCGGCGTGCCGATGCTC
GTGCTCACGCCTTCGCACAAGACGGCGCGCAAGGTCGGGCTGCTGTGGGGCGCCCATGCCGTCAGCACCAAGGACATCGG
CAGCTTCGAGGAGATGATCGCCAAGGGCAAGCGCATGGCGCTGCGCCACGGCTTCGGCTCGGCCGGATCCAAGCTCGTCG
CGCTGGCGGGTGTTCCGTTCGGCACGCCGGGGGCGACCAACCTGCTCCACGTGGTGACGCTTACCGGCGACGAACTCACC
CGCCACGGCGAATGA

Upstream 100 bases:

>100_bases
AGTTCATCGATTGCATTCGTATCCACTGTAAACGTCCTTGGGTTTTGCACGGCCATCGGCCATAGCGCGTTTCGCCGGAA
TGATAAGGAGTACCAAAGCC

Downstream 100 bases:

>100_bases
GGCGACGATAGACGGCCGGTCGCGCGTCAGCGCGTGCCGGTCCCTGTCGGTCGGGCTCAGCGCTCTCCGAACAGTGCGGA
GCCGACGCGCACATGCGTCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MAKLDPRGRKVKILATVGPASRDPEMLEKLFRAGADAFRVNMSHGEHAIHAETIANIRALEKKVNRPITILCDLQGPKLR
VGKFKDGRAVIRHSGHFTLDRNPEPGDETRVCLPHPELFGVLHKGQRLLIDDGKLRLRVIRADENEILCSAEVGGVISDR
KGVNVPDAVVPVPALTDKDRRDLSFAVEQGADWIALSFVQRPEDVAEARRLLGGHGSLMAKIEKPAAISRLEEIVELSDG
IMVARGDLGVELNPEEVPPLQKRIVETARRQGKPVVVATQMLESMIEAPTPTRAEVSDVANAVYDGADAVMLSAETAAGA
WPEEAVTIMHRIAAQVEADPGYGARVHFLETLPDPTTADALAQAAASITETLPISGIIVFTGSGSTARRVARERPGVPML
VLTPSHKTARKVGLLWGAHAVSTKDIGSFEEMIAKGKRMALRHGFGSAGSKLVALAGVPFGTPGATNLLHVVTLTGDELT
RHGE

Sequences:

>Translated_484_residues
MAKLDPRGRKVKILATVGPASRDPEMLEKLFRAGADAFRVNMSHGEHAIHAETIANIRALEKKVNRPITILCDLQGPKLR
VGKFKDGRAVIRHSGHFTLDRNPEPGDETRVCLPHPELFGVLHKGQRLLIDDGKLRLRVIRADENEILCSAEVGGVISDR
KGVNVPDAVVPVPALTDKDRRDLSFAVEQGADWIALSFVQRPEDVAEARRLLGGHGSLMAKIEKPAAISRLEEIVELSDG
IMVARGDLGVELNPEEVPPLQKRIVETARRQGKPVVVATQMLESMIEAPTPTRAEVSDVANAVYDGADAVMLSAETAAGA
WPEEAVTIMHRIAAQVEADPGYGARVHFLETLPDPTTADALAQAAASITETLPISGIIVFTGSGSTARRVARERPGVPML
VLTPSHKTARKVGLLWGAHAVSTKDIGSFEEMIAKGKRMALRHGFGSAGSKLVALAGVPFGTPGATNLLHVVTLTGDELT
RHGE
>Mature_483_residues
AKLDPRGRKVKILATVGPASRDPEMLEKLFRAGADAFRVNMSHGEHAIHAETIANIRALEKKVNRPITILCDLQGPKLRV
GKFKDGRAVIRHSGHFTLDRNPEPGDETRVCLPHPELFGVLHKGQRLLIDDGKLRLRVIRADENEILCSAEVGGVISDRK
GVNVPDAVVPVPALTDKDRRDLSFAVEQGADWIALSFVQRPEDVAEARRLLGGHGSLMAKIEKPAAISRLEEIVELSDGI
MVARGDLGVELNPEEVPPLQKRIVETARRQGKPVVVATQMLESMIEAPTPTRAEVSDVANAVYDGADAVMLSAETAAGAW
PEEAVTIMHRIAAQVEADPGYGARVHFLETLPDPTTADALAQAAASITETLPISGIIVFTGSGSTARRVARERPGVPMLV
LTPSHKTARKVGLLWGAHAVSTKDIGSFEEMIAKGKRMALRHGFGSAGSKLVALAGVPFGTPGATNLLHVVTLTGDELTR
HGE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=40, Blast_Score=301, Evalue=8e-82,
Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=38.6036960985626, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=38.6036960985626, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=38.3838383838384, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=38.3838383838384, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=38.3275261324042, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=38.3275261324042, Blast_Score=190, Evalue=3e-48,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=35.0210970464135, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=35.0210970464135, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI310128738, Length=209, Percent_Identity=34.4497607655502, Blast_Score=122, Evalue=7e-28,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=38.4778012684989, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=36.0587002096436, Blast_Score=274, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=38.1147540983607, Blast_Score=304, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI71984406, Length=502, Percent_Identity=38.0478087649402, Blast_Score=275, Evalue=4e-74,
Organism=Caenorhabditis elegans, GI71984413, Length=502, Percent_Identity=38.0478087649402, Blast_Score=275, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17506829, Length=502, Percent_Identity=38.0478087649402, Blast_Score=275, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17506831, Length=502, Percent_Identity=38.0478087649402, Blast_Score=274, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=35.3909465020576, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=33.9506172839506, Blast_Score=256, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=37.3225152129817, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=37.3225152129817, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24648966, Length=412, Percent_Identity=37.378640776699, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24581235, Length=428, Percent_Identity=32.2429906542056, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24646914, Length=334, Percent_Identity=35.0299401197605, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51960; Mature: 51829

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLDPRGRKVKILATVGPASRDPEMLEKLFRAGADAFRVNMSHGEHAIHAETIANIRAL
CCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHH
EKKVNRPITILCDLQGPKLRVGKFKDGRAVIRHSGHFTLDRNPEPGDETRVCLPHPELFG
HHHHCCCEEEEEECCCCEEEECCCCCCCEEEEECCEEEECCCCCCCCCCEEECCCHHHHH
VLHKGQRLLIDDGKLRLRVIRADENEILCSAEVGGVISDRKGVNVPDAVVPVPALTDKDR
HHHCCCEEEEECCCEEEEEEEECCCCEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCC
RDLSFAVEQGADWIALSFVQRPEDVAEARRLLGGHGSLMAKIEKPAAISRLEEIVELSDG
HHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCC
IMVARGDLGVELNPEEVPPLQKRIVETARRQGKPVVVATQMLESMIEAPTPTRAEVSDVA
EEEEECCCCCEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHH
NAVYDGADAVMLSAETAAGAWPEEAVTIMHRIAAQVEADPGYGARVHFLETLPDPTTADA
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCHHHH
LAQAAASITETLPISGIIVFTGSGSTARRVARERPGVPMLVLTPSHKTARKVGLLWGAHA
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHEECCCC
VSTKDIGSFEEMIAKGKRMALRHGFGSAGSKLVALAGVPFGTPGATNLLHVVTLTGDELT
CCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHH
RHGE
CCCC
>Mature Secondary Structure 
AKLDPRGRKVKILATVGPASRDPEMLEKLFRAGADAFRVNMSHGEHAIHAETIANIRAL
CCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHH
EKKVNRPITILCDLQGPKLRVGKFKDGRAVIRHSGHFTLDRNPEPGDETRVCLPHPELFG
HHHHCCCEEEEEECCCCEEEECCCCCCCEEEEECCEEEECCCCCCCCCCEEECCCHHHHH
VLHKGQRLLIDDGKLRLRVIRADENEILCSAEVGGVISDRKGVNVPDAVVPVPALTDKDR
HHHCCCEEEEECCCEEEEEEEECCCCEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCC
RDLSFAVEQGADWIALSFVQRPEDVAEARRLLGGHGSLMAKIEKPAAISRLEEIVELSDG
HHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCC
IMVARGDLGVELNPEEVPPLQKRIVETARRQGKPVVVATQMLESMIEAPTPTRAEVSDVA
EEEEECCCCCEECCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCHHHHHHH
NAVYDGADAVMLSAETAAGAWPEEAVTIMHRIAAQVEADPGYGARVHFLETLPDPTTADA
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCHHHH
LAQAAASITETLPISGIIVFTGSGSTARRVARERPGVPMLVLTPSHKTARKVGLLWGAHA
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHEECCCC
VSTKDIGSFEEMIAKGKRMALRHGFGSAGSKLVALAGVPFGTPGATNLLHVVTLTGDELT
CCCCCCCHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHH
RHGE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA