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The map label for this gene is trpB2 [H]

Identifier: 86750824

GI number: 86750824

Start: 4252697

End: 4254061

Strand: Reverse

Name: trpB2 [H]

Synonym: RPB_3714

Alternate gene names: 86750824

Gene position: 4254061-4252697 (Counterclockwise)

Preceding gene: 86750826

Following gene: 86750823

Centisome position: 79.79

GC content: 66.67

Gene sequence:

>1365_bases
ATGAGCGACCAGATCAAGTACGTCCTGGACGAGGAGAACATCCCGAAGTCCTGGTACAATCTCAATGCCGACTTTCCCAA
GCCGGTACCCGACGTGCTGCATCCGGGCACGCATCAGCCGGTCGGCCCGTCCGATCTGGAGCCGCTGTTTCCGATGGAGC
TGATCCTCCAGGAGGTCGCCACCGATCGCTACATCGACATTCCCGCGCCGGTGCGCGACGTGTTCCGGATGTGGCGGCCG
TCGCCGCTGGTGCGTGCGCGCCGCCTCGAGCAGGCGCTCGGCACGCCGGCCAAGATCTACTACAAATACGAAGGCGTCTC
GCCGGCCGGCTCGCACAAGCCGAACACCGCGGTGCCGCAGGCCTGGTACAACAAAGAGGCCGGCATCAAGAAGCTGTCGA
CCGAGACCGGTGCCGGGCAGTGGGGCTCGTCGCTGGCCTTCGCCGGCTCGCTGTTCGGGCTCGACGTGCTGGTGTTCCAG
GTCCGCGTCTCGTTCGACCAGAAACCGTATCGCCGCGCGCTGATGGAAACCTACGGCGCGCGCTGCATCGCCTCGCCCTC
GACCGAGACCGAGTCCGGCCGCGCCATCCTGGCGCAGCATCCCGACAGCCCCGGCTCGCTCGGCATCGCGATCTCCGAAG
CCGTCGAAGTCGCGGCGAAGAATCCGGATATCAAATACGCGCTCGGCTCGGTGCTCAATCACGTCATGCTGCACCAGACC
ATCATCGGCCAGGAAGCGATCAAGCAGTGCGAAATGGCCGGTGACGATCCCGACGTGATCATCGGCTGCGCCGGCGGCGG
CTCGAATTTCGCAGGCCTTGCGTTCCCGTTCCTCGGCCTGCAGCTGCGCGGCGGCCGGTCGCGGCGGATCATCGCGGTCG
AGCCCGCGGCGTGCCCGACGCTGACGCGCGGCACCTACGCCTATGATTTCGGCGACACCGCGCATCTGACGCCCTTGGTG
AAGATGCACACGCTGGGCTCGACCTTCATTCCGCCGGGCTTCCACGCCGGCGGCCTGCGCTATCACGGCATGAGCGGGAT
GGTGTCGCACGCCTACGAGCTCGGCCTGATCGAGGCGCGTGCCTATCACCAGGTGAAGTGCTTCGAAGCCGGCGTGCAGT
TCGCCCGCAACGAGGGCATCGTGCCGGCGCCGGAATCGACCCACGCGGTGCGCTGCGCGATCGACGAGGCGCTGCGCTGC
AAGGCGGAGGGCAAGGCGGAGACGATCCTGTTCAACCTCTCGGGTCACGGCCATTTCGACATGCAGGCCTACATCAACTA
CTACGAAGGCAAGCTCGTCGACGTCGACTACAACGAAGCCGACCTCGCGACCGCGCTGGCGGGCCTGCCGGCGGTGGCGG
CTTAG

Upstream 100 bases:

>100_bases
GCGGGCGGCGTGATTCAATTGACACGGCGCAATTGGCAGGCCGCCGGCCGCTGTATTACCCTCCCGGCCGCACCAAGGGG
CCGCGTGAGGGGAGACCAGA

Downstream 100 bases:

>100_bases
TCTCGAGCCGGCCGCAAGGCACGGACCAAACCTCTCCCCGCCATGCGGGGAGAGGTCGGATTGCGCAGCAATCCGGGTGA
GGGGCCGGGCACGGCCGTCG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSDQIKYVLDEENIPKSWYNLNADFPKPVPDVLHPGTHQPVGPSDLEPLFPMELILQEVATDRYIDIPAPVRDVFRMWRP
SPLVRARRLEQALGTPAKIYYKYEGVSPAGSHKPNTAVPQAWYNKEAGIKKLSTETGAGQWGSSLAFAGSLFGLDVLVFQ
VRVSFDQKPYRRALMETYGARCIASPSTETESGRAILAQHPDSPGSLGIAISEAVEVAAKNPDIKYALGSVLNHVMLHQT
IIGQEAIKQCEMAGDDPDVIIGCAGGGSNFAGLAFPFLGLQLRGGRSRRIIAVEPAACPTLTRGTYAYDFGDTAHLTPLV
KMHTLGSTFIPPGFHAGGLRYHGMSGMVSHAYELGLIEARAYHQVKCFEAGVQFARNEGIVPAPESTHAVRCAIDEALRC
KAEGKAETILFNLSGHGHFDMQAYINYYEGKLVDVDYNEADLATALAGLPAVAA

Sequences:

>Translated_454_residues
MSDQIKYVLDEENIPKSWYNLNADFPKPVPDVLHPGTHQPVGPSDLEPLFPMELILQEVATDRYIDIPAPVRDVFRMWRP
SPLVRARRLEQALGTPAKIYYKYEGVSPAGSHKPNTAVPQAWYNKEAGIKKLSTETGAGQWGSSLAFAGSLFGLDVLVFQ
VRVSFDQKPYRRALMETYGARCIASPSTETESGRAILAQHPDSPGSLGIAISEAVEVAAKNPDIKYALGSVLNHVMLHQT
IIGQEAIKQCEMAGDDPDVIIGCAGGGSNFAGLAFPFLGLQLRGGRSRRIIAVEPAACPTLTRGTYAYDFGDTAHLTPLV
KMHTLGSTFIPPGFHAGGLRYHGMSGMVSHAYELGLIEARAYHQVKCFEAGVQFARNEGIVPAPESTHAVRCAIDEALRC
KAEGKAETILFNLSGHGHFDMQAYINYYEGKLVDVDYNEADLATALAGLPAVAA
>Mature_453_residues
SDQIKYVLDEENIPKSWYNLNADFPKPVPDVLHPGTHQPVGPSDLEPLFPMELILQEVATDRYIDIPAPVRDVFRMWRPS
PLVRARRLEQALGTPAKIYYKYEGVSPAGSHKPNTAVPQAWYNKEAGIKKLSTETGAGQWGSSLAFAGSLFGLDVLVFQV
RVSFDQKPYRRALMETYGARCIASPSTETESGRAILAQHPDSPGSLGIAISEAVEVAAKNPDIKYALGSVLNHVMLHQTI
IGQEAIKQCEMAGDDPDVIIGCAGGGSNFAGLAFPFLGLQLRGGRSRRIIAVEPAACPTLTRGTYAYDFGDTAHLTPLVK
MHTLGSTFIPPGFHAGGLRYHGMSGMVSHAYELGLIEARAYHQVKCFEAGVQFARNEGIVPAPESTHAVRCAIDEALRCK
AEGKAETILFNLSGHGHFDMQAYINYYEGKLVDVDYNEADLATALAGLPAVAA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=411, Percent_Identity=26.0340632603406, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321412, Length=365, Percent_Identity=28.4931506849315, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 49225; Mature: 49093

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQIKYVLDEENIPKSWYNLNADFPKPVPDVLHPGTHQPVGPSDLEPLFPMELILQEVA
CCCCEEEEECCCCCCHHHEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
TDRYIDIPAPVRDVFRMWRPSPLVRARRLEQALGTPAKIYYKYEGVSPAGSHKPNTAVPQ
CCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCH
AWYNKEAGIKKLSTETGAGQWGSSLAFAGSLFGLDVLVFQVRVSFDQKPYRRALMETYGA
HHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
RCIASPSTETESGRAILAQHPDSPGSLGIAISEAVEVAAKNPDIKYALGSVLNHVMLHQT
EEEECCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IIGQEAIKQCEMAGDDPDVIIGCAGGGSNFAGLAFPFLGLQLRGGRSRRIIAVEPAACPT
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHCEEECCCCCCEEEEECCCCCCC
LTRGTYAYDFGDTAHLTPLVKMHTLGSTFIPPGFHAGGLRYHGMSGMVSHAYELGLIEAR
CCCCCEEEECCCCCHHCHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCEEEHH
AYHQVKCFEAGVQFARNEGIVPAPESTHAVRCAIDEALRCKAEGKAETILFNLSGHGHFD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCCCEEEEEEEECCCCCEE
MQAYINYYEGKLVDVDYNEADLATALAGLPAVAA
HHHHEEEECCEEEEECCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDQIKYVLDEENIPKSWYNLNADFPKPVPDVLHPGTHQPVGPSDLEPLFPMELILQEVA
CCCEEEEECCCCCCHHHEECCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHH
TDRYIDIPAPVRDVFRMWRPSPLVRARRLEQALGTPAKIYYKYEGVSPAGSHKPNTAVPQ
CCCEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCH
AWYNKEAGIKKLSTETGAGQWGSSLAFAGSLFGLDVLVFQVRVSFDQKPYRRALMETYGA
HHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
RCIASPSTETESGRAILAQHPDSPGSLGIAISEAVEVAAKNPDIKYALGSVLNHVMLHQT
EEEECCCCCCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IIGQEAIKQCEMAGDDPDVIIGCAGGGSNFAGLAFPFLGLQLRGGRSRRIIAVEPAACPT
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHCEEECCCCCCEEEEECCCCCCC
LTRGTYAYDFGDTAHLTPLVKMHTLGSTFIPPGFHAGGLRYHGMSGMVSHAYELGLIEAR
CCCCCEEEECCCCCHHCHHHHHHHCCCCCCCCCCCCCCEEECCCCHHHHHHHHHCEEEHH
AYHQVKCFEAGVQFARNEGIVPAPESTHAVRCAIDEALRCKAEGKAETILFNLSGHGHFD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCCCEEEEEEEECCCCCEE
MQAYINYYEGKLVDVDYNEADLATALAGLPAVAA
HHHHEEEECCEEEEECCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]