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The map label for this gene is adhI [H]

Identifier: 86750529

GI number: 86750529

Start: 3906995

End: 3908104

Strand: Direct

Name: adhI [H]

Synonym: RPB_3418

Alternate gene names: 86750529

Gene position: 3906995-3908104 (Clockwise)

Preceding gene: 86750528

Following gene: 86750537

Centisome position: 73.28

GC content: 65.23

Gene sequence:

>1110_bases
ATGAAGACCCGCGCCGCCGTCGCATTCGAGGCCAAGAGGCCGCTCGAAATCGTCGAACTGGATCTCGACGGGCCGAAGGC
CGGCGAAGTGCTGGTCGAGATCAAGGCGACCGGGATCTGCCACACCGACGCCTATACGCTCGACGGCCTCGACTCCGAAG
GCATCTTCCCCTCGATCCTCGGCCACGAGGGCGCGGGCATCGTGCGCGAGGTCGGCGCCGGCGTCACCTCGGTGAAGCCC
GGCGATCACGTCATTCCGCTGTACACGCCGGAGTGCCGGCAGTGCAAAAGCTGCCTGAGCCAGAAGACCAATCTGTGCAC
CTCGATCCGCGCCACCCAGGGCAAGGGCGTGATGCCGGACGGCACCTCGCGCTTCAGCTATCAGGGCAAGCCGATCTATC
ATTACATGGGCTGCTCGACGTTTTCGAACTTCACCGTGCTGCCGGAGATCGCGCTGGCGAAGATCCGCGACGACGCGCCG
TTCGACAAGAGCTGCTACATCGGCTGCGGCGTCACCACCGGCGTCGGCGCGGTGGTCAACACCGCGAAGGTGACGCCCGG
CTCCAATGTCGTGGTGTTCGGCCTCGGCGGCATCGGCCTCAACGTCATTCAGGGCGCGCGGATGGTCGGCGCCGACAAGA
TCGTCGGCGTCGACATCAACGACGACAAGGAGGAATGGGGCCGCCGCTTCGGCATGACGCATTTCGTCAATCCGAAGACA
ATCGACGGCGACATCGTCCAGCACCTCGTCGGCCTGACCGACGGCGGCGCCGACTACACGTTCGACTGCACCGGCAACAC
CACTGTGATGCGCCAGGCGCTGGAAGCCTGCCACCGCGGCTGGGGCGTCTCGGTGGTGATCGGCGTCGCCGAAGCCGGCA
AGGAAATCTCGACGCGGCCGTTCCAGCTCGTCACCGGCCGGGTCTGGAAAGGCAGCGCCTTCGGCGGCGCCCGCGGCCGC
ACCGACGTGCCGAAAATCGTCGACTGGTACATGAACGGCAAGATCGAGATCGACCCGATGATCACCCACGTGCTCAAGCT
CGAGGAGATCAACAAGGGTTTCGAGCTGATGCACGAGGGCAAGTCGATCCGGTCGGTGGTGGTGTTCTAG

Upstream 100 bases:

>100_bases
GCCGCATTGATCAGGCGTTCAAAAAACAGTTCCTGTTCTCCCGCCGATTTGCCATACGGAAGGCCAATCGCAACGACACT
GATGCAGGGAGAGACCAGCC

Downstream 100 bases:

>100_bases
ACTTTTCCGCCGTCATTCCGGGGCGCTCACGATAGTGAGCGAACCCGGAATCCCGAGGTTCATGGCACCGCGAGATTCCG
GGTTCGCGCTGTGCGCGCCC

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKTRAAVAFEAKRPLEIVELDLDGPKAGEVLVEIKATGICHTDAYTLDGLDSEGIFPSILGHEGAGIVREVGAGVTSVKP
GDHVIPLYTPECRQCKSCLSQKTNLCTSIRATQGKGVMPDGTSRFSYQGKPIYHYMGCSTFSNFTVLPEIALAKIRDDAP
FDKSCYIGCGVTTGVGAVVNTAKVTPGSNVVVFGLGGIGLNVIQGARMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKT
IDGDIVQHLVGLTDGGADYTFDCTGNTTVMRQALEACHRGWGVSVVIGVAEAGKEISTRPFQLVTGRVWKGSAFGGARGR
TDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFELMHEGKSIRSVVVF

Sequences:

>Translated_369_residues
MKTRAAVAFEAKRPLEIVELDLDGPKAGEVLVEIKATGICHTDAYTLDGLDSEGIFPSILGHEGAGIVREVGAGVTSVKP
GDHVIPLYTPECRQCKSCLSQKTNLCTSIRATQGKGVMPDGTSRFSYQGKPIYHYMGCSTFSNFTVLPEIALAKIRDDAP
FDKSCYIGCGVTTGVGAVVNTAKVTPGSNVVVFGLGGIGLNVIQGARMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKT
IDGDIVQHLVGLTDGGADYTFDCTGNTTVMRQALEACHRGWGVSVVIGVAEAGKEISTRPFQLVTGRVWKGSAFGGARGR
TDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFELMHEGKSIRSVVVF
>Mature_369_residues
MKTRAAVAFEAKRPLEIVELDLDGPKAGEVLVEIKATGICHTDAYTLDGLDSEGIFPSILGHEGAGIVREVGAGVTSVKP
GDHVIPLYTPECRQCKSCLSQKTNLCTSIRATQGKGVMPDGTSRFSYQGKPIYHYMGCSTFSNFTVLPEIALAKIRDDAP
FDKSCYIGCGVTTGVGAVVNTAKVTPGSNVVVFGLGGIGLNVIQGARMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKT
IDGDIVQHLVGLTDGGADYTFDCTGNTTVMRQALEACHRGWGVSVVIGVAEAGKEISTRPFQLVTGRVWKGSAFGGARGR
TDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFELMHEGKSIRSVVVF

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=61.0354223433242, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI71565152, Length=373, Percent_Identity=45.8445040214477, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.3414634146341, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=47.1544715447154, Blast_Score=352, Evalue=2e-97,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=47.1544715447154, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=46.070460704607, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.5284552845528, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI156523966, Length=369, Percent_Identity=43.6314363143631, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=44.0443213296399, Blast_Score=330, Evalue=1e-90,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=61.7886178861789, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI87082125, Length=382, Percent_Identity=26.4397905759162, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788073, Length=364, Percent_Identity=26.0989010989011, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1790045, Length=354, Percent_Identity=28.2485875706215, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787863, Length=360, Percent_Identity=25, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788075, Length=364, Percent_Identity=26.9230769230769, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081918, Length=345, Percent_Identity=29.2753623188406, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI226510992, Length=365, Percent_Identity=26.8493150684932, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786825, Length=378, Percent_Identity=24.8677248677249, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788407, Length=225, Percent_Identity=24.8888888888889, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=25.3405994550409, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=55.4959785522788, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=55.2, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=55.3935860058309, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17562584, Length=371, Percent_Identity=30.4582210242588, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71988145, Length=372, Percent_Identity=28.494623655914, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17562582, Length=373, Percent_Identity=28.4182305630027, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562876, Length=357, Percent_Identity=25.4901960784314, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=57.4123989218329, Blast_Score=452, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6323099, Length=387, Percent_Identity=28.6821705426357, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322619, Length=354, Percent_Identity=28.8135593220339, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319955, Length=354, Percent_Identity=28.8135593220339, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319621, Length=370, Percent_Identity=27.027027027027, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324486, Length=371, Percent_Identity=26.1455525606469, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323729, Length=373, Percent_Identity=26.5415549597855, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=363, Percent_Identity=24.5179063360882, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=276, Percent_Identity=27.536231884058, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=58.695652173913, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI17137530, Length=337, Percent_Identity=27.5964391691395, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 39570; Mature: 39570

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRAAVAFEAKRPLEIVELDLDGPKAGEVLVEIKATGICHTDAYTLDGLDSEGIFPSIL
CCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVREVGAGVTSVKPGDHVIPLYTPECRQCKSCLSQKTNLCTSIRATQGKGVMPD
CCCCCCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
GTSRFSYQGKPIYHYMGCSTFSNFTVLPEIALAKIRDDAPFDKSCYIGCGVTTGVGAVVN
CCCCCCCCCCEEEEEECCCCCCCCEECHHHHHHHHCCCCCCCCCEEEECCCHHCCHHHEE
TAKVTPGSNVVVFGLGGIGLNVIQGARMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKT
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCCCHHHHHHHCCCEEECCCCC
IDGDIVQHLVGLTDGGADYTFDCTGNTTVMRQALEACHRGWGVSVVIGVAEAGKEISTRP
CCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
FQLVTGRVWKGSAFGGARGRTDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFELMHEG
EEEEECEEEECCCCCCCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHCCHHHHHHCC
KSIRSVVVF
CCCEEEEEC
>Mature Secondary Structure
MKTRAAVAFEAKRPLEIVELDLDGPKAGEVLVEIKATGICHTDAYTLDGLDSEGIFPSIL
CCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVREVGAGVTSVKPGDHVIPLYTPECRQCKSCLSQKTNLCTSIRATQGKGVMPD
CCCCCCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
GTSRFSYQGKPIYHYMGCSTFSNFTVLPEIALAKIRDDAPFDKSCYIGCGVTTGVGAVVN
CCCCCCCCCCEEEEEECCCCCCCCEECHHHHHHHHCCCCCCCCCEEEECCCHHCCHHHEE
TAKVTPGSNVVVFGLGGIGLNVIQGARMVGADKIVGVDINDDKEEWGRRFGMTHFVNPKT
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCCCHHHHHHHCCCEEECCCCC
IDGDIVQHLVGLTDGGADYTFDCTGNTTVMRQALEACHRGWGVSVVIGVAEAGKEISTRP
CCHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCC
FQLVTGRVWKGSAFGGARGRTDVPKIVDWYMNGKIEIDPMITHVLKLEEINKGFELMHEG
EEEEECEEEECCCCCCCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHCCHHHHHHCC
KSIRSVVVF
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]