Click here to switch to the map view.

The map label for this gene is yoaE [H]

Identifier: 86750413

GI number: 86750413

Start: 3773005

End: 3775098

Strand: Direct

Name: yoaE [H]

Synonym: RPB_3302

Alternate gene names: 86750413

Gene position: 3773005-3775098 (Clockwise)

Preceding gene: 86750396

Following gene: 86750416

Centisome position: 70.77

GC content: 66.19

Gene sequence:

>2094_bases
ATGAACCAGACACGCGCGAACATCACGATCGGCAGAACGGTTTGTCCGCACGACTGCCCCTCGGCGTGCGCACTCGACGT
CGAACTGCTCGATGACGACCGCATCGGGCGCATCCGCGGCGCCCGCGACAACTCCTACACGCTCGGCGTGATCTGCGAGA
AAGTCGCGCGCTACGCCGAGCGCTTTCATCATCCAGACCGTCTGCTACATCCCCTGAAACGCCGCGGTCCCAAGGGGACC
GGCGATTTCGTACGCATTTCCTGGGAGGAAGCGCTCGATCTCACCGCGACGGCATTCACGACGGCCGAGCGGGACCACGG
CGCCGAAAGCGTCTGGCCGTTCTACTTCGCCGGCACGATGGGCCTCGTCATGCGCGACGGAATCGAACGGCTGCGCAATG
CCAAGGGCTATTCCGGCATGTACGCCACCTATTGCACGCCGTTGTCGTGGGCGGGTTACTACGCGGGCGTCGGCCGGCTG
ACAGGGGTCGACCCGCGCGAGATGAGGCACTCGGACCTGATCGTGGTCTGGGGCGGCAATCCGGTGAACACGCAGGTCAA
CGTGATGACGCATGTGGGCATCGCCCGCAAGACTCGCGGCGCCAAGCTCGCCTGCGTCGACGTCTACGACACCGGCACCA
TGAAGCAGGCCGATATCAAGCTGCTGATCCGGCCGGGCACCGATGGTGCGCTGGCCTGCGCGGTGATGCACGTACTGTTT
CGCGACGGCTACGCGGATCGCGAATATCTGCATCGGTACACCGATTGCCCGGATGCGTTCGAGCGCCACCTCGACGACAA
GACGCCGCAATGGGCGTCGCGCATCTGCGATATCCCGGCGACCGAGATCGAGGCTTTCGCGGCGCTCATCGGCGCCACAC
CCCGCAGCTTCTTCCGGATCGGTTACGGCTTCACCCGCTCGCGCAACGGTGCCGTGAACATTCATGCGGTGAGTTCGATT
CCGGCCGTCACCGGTGCATGGCGCCACCAAGGCGGCGGCGCGCTGCATACCAACAGCGGCCTCTACAAATGGGACAAGAC
CTTGATCGAGGGTCGCGACGCCGGCGTCGCGCCGGCCCGGATGCTCGATCAATCACGGATCGGCGCGGTTCTGCTGGGCG
ATCCACACGACCTCCAGGGCGGCCCGCCGGTCAAGGCGATGCTGATCCAGAACACCAACCCCGCATCGGTGGCGCCCGAT
CAGACCAGCGTGAAACGCGGACTGGCGCGTGACGACCTGTTTGTCTGCGTGCACGAACAGTTCATGACCGAAACCGCGCG
CTATGCCGATGTCGTTTTGCCGGCGACGATGTTCCTCGAGCACGACGACGTCTATCAGGGAGGCGGCCACCAGCACATCC
TGCTCGGCCGCCGGCTGGTCCCGCCAGCCGGCGAGTGCCGCAGCAATCACGAGGTGATCAGCGCCATCGCCGATCGTGTC
GGCGCGACGCATCGCGGATTCGCGATGACTCCGCGCGACATCATCGAGGCGACGCTCGCGGCGTCACACCGGCCGGGGCT
CGATCGGCTGGAGGTCGACGGCTGGCTCGACGTTCAGACCGACTTCGCGACGGCGCACAACCTCGATGGGTTCGGCCATC
CCGACGGCAAGTATCACTTCAAGCCGGATTGGCAGCAGACCGCCGCCTATCTCGGAACCCGCACGCTCGGTCCGTGGGAG
GCCCTGCCCCCGTTTCCGGATCATTGCTCGCTGATCGAAGACGCCACCGACGAATATCCGTTCCGCCTCGCCACCAGTCC
GGCCCGCACCTTCCTCAATTCGACGTTCAACGAGACGGCAACGAGCAAGACGCGCGAGGGCCGCCCCACCGTGATGGTGC
ATCCCGCCGATCTCACCGCCCTCGGCCTGACCTCCGGAGACAAGGTGAAGCTCGGAAGCGCGCGCGGCGAGGTCACGCTG
CATTGCCGGGCCAACGAGGGCCAGCGCCGCGGCGTGCTGATCGCCGAATCGATCTGGCCCAACGACGCCCACGAGGACGG
GTGCGGCATCAACACCCTGACCGGCGCCGACCAGTCGGCCCCGGCCGGCGGCGGCAGTTTCCACGACAACCGGGTGTGGA
TCAGGAAGGCTTGA

Upstream 100 bases:

>100_bases
CGGACTCGCACGGATAGCAGCCGATCGCGCGCGCCTCATCGCGGGCTCCCACGTCTCCCGCTCGATTGCGGATCGGCGCG
GCGACGACTAGGTTGGCGCG

Downstream 100 bases:

>100_bases
CCGGCGCCGGATGCGCGCCGGGCGCGGAGTCGCGCGATCCCTGGGCGCTCGGCGCGACGGGATCGATGGTCGCCTCATCG
TGCTGCGTCAGCGGGCGGTT

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 697

Protein sequence:

>697_residues
MNQTRANITIGRTVCPHDCPSACALDVELLDDDRIGRIRGARDNSYTLGVICEKVARYAERFHHPDRLLHPLKRRGPKGT
GDFVRISWEEALDLTATAFTTAERDHGAESVWPFYFAGTMGLVMRDGIERLRNAKGYSGMYATYCTPLSWAGYYAGVGRL
TGVDPREMRHSDLIVVWGGNPVNTQVNVMTHVGIARKTRGAKLACVDVYDTGTMKQADIKLLIRPGTDGALACAVMHVLF
RDGYADREYLHRYTDCPDAFERHLDDKTPQWASRICDIPATEIEAFAALIGATPRSFFRIGYGFTRSRNGAVNIHAVSSI
PAVTGAWRHQGGGALHTNSGLYKWDKTLIEGRDAGVAPARMLDQSRIGAVLLGDPHDLQGGPPVKAMLIQNTNPASVAPD
QTSVKRGLARDDLFVCVHEQFMTETARYADVVLPATMFLEHDDVYQGGGHQHILLGRRLVPPAGECRSNHEVISAIADRV
GATHRGFAMTPRDIIEATLAASHRPGLDRLEVDGWLDVQTDFATAHNLDGFGHPDGKYHFKPDWQQTAAYLGTRTLGPWE
ALPPFPDHCSLIEDATDEYPFRLATSPARTFLNSTFNETATSKTREGRPTVMVHPADLTALGLTSGDKVKLGSARGEVTL
HCRANEGQRRGVLIAESIWPNDAHEDGCGINTLTGADQSAPAGGGSFHDNRVWIRKA

Sequences:

>Translated_697_residues
MNQTRANITIGRTVCPHDCPSACALDVELLDDDRIGRIRGARDNSYTLGVICEKVARYAERFHHPDRLLHPLKRRGPKGT
GDFVRISWEEALDLTATAFTTAERDHGAESVWPFYFAGTMGLVMRDGIERLRNAKGYSGMYATYCTPLSWAGYYAGVGRL
TGVDPREMRHSDLIVVWGGNPVNTQVNVMTHVGIARKTRGAKLACVDVYDTGTMKQADIKLLIRPGTDGALACAVMHVLF
RDGYADREYLHRYTDCPDAFERHLDDKTPQWASRICDIPATEIEAFAALIGATPRSFFRIGYGFTRSRNGAVNIHAVSSI
PAVTGAWRHQGGGALHTNSGLYKWDKTLIEGRDAGVAPARMLDQSRIGAVLLGDPHDLQGGPPVKAMLIQNTNPASVAPD
QTSVKRGLARDDLFVCVHEQFMTETARYADVVLPATMFLEHDDVYQGGGHQHILLGRRLVPPAGECRSNHEVISAIADRV
GATHRGFAMTPRDIIEATLAASHRPGLDRLEVDGWLDVQTDFATAHNLDGFGHPDGKYHFKPDWQQTAAYLGTRTLGPWE
ALPPFPDHCSLIEDATDEYPFRLATSPARTFLNSTFNETATSKTREGRPTVMVHPADLTALGLTSGDKVKLGSARGEVTL
HCRANEGQRRGVLIAESIWPNDAHEDGCGINTLTGADQSAPAGGGSFHDNRVWIRKA
>Mature_697_residues
MNQTRANITIGRTVCPHDCPSACALDVELLDDDRIGRIRGARDNSYTLGVICEKVARYAERFHHPDRLLHPLKRRGPKGT
GDFVRISWEEALDLTATAFTTAERDHGAESVWPFYFAGTMGLVMRDGIERLRNAKGYSGMYATYCTPLSWAGYYAGVGRL
TGVDPREMRHSDLIVVWGGNPVNTQVNVMTHVGIARKTRGAKLACVDVYDTGTMKQADIKLLIRPGTDGALACAVMHVLF
RDGYADREYLHRYTDCPDAFERHLDDKTPQWASRICDIPATEIEAFAALIGATPRSFFRIGYGFTRSRNGAVNIHAVSSI
PAVTGAWRHQGGGALHTNSGLYKWDKTLIEGRDAGVAPARMLDQSRIGAVLLGDPHDLQGGPPVKAMLIQNTNPASVAPD
QTSVKRGLARDDLFVCVHEQFMTETARYADVVLPATMFLEHDDVYQGGGHQHILLGRRLVPPAGECRSNHEVISAIADRV
GATHRGFAMTPRDIIEATLAASHRPGLDRLEVDGWLDVQTDFATAHNLDGFGHPDGKYHFKPDWQQTAAYLGTRTLGPWE
ALPPFPDHCSLIEDATDEYPFRLATSPARTFLNSTFNETATSKTREGRPTVMVHPADLTALGLTSGDKVKLGSARGEVTL
HCRANEGQRRGVLIAESIWPNDAHEDGCGINTLTGADQSAPAGGGSFHDNRVWIRKA

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=785, Percent_Identity=25.9872611464968, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1787870, Length=792, Percent_Identity=24.2424242424242, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI171474008, Length=766, Percent_Identity=24.8041775456919, Blast_Score=162, Evalue=8e-41,
Organism=Escherichia coli, GI145693196, Length=457, Percent_Identity=26.4770240700219, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI3868721, Length=683, Percent_Identity=22.8404099560761, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI87081994, Length=691, Percent_Identity=23.0101302460203, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1787231, Length=683, Percent_Identity=21.9619326500732, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 76389; Mature: 76389

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQTRANITIGRTVCPHDCPSACALDVELLDDDRIGRIRGARDNSYTLGVICEKVARYAE
CCCCCCEEEECCEECCCCCCCHHEEEEEEECCCCCCCEECCCCCCEEHHHHHHHHHHHHH
RFHHPDRLLHPLKRRGPKGTGDFVRISWEEALDLTATAFTTAERDHGAESVWPFYFAGTM
HHCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHCEEHHHEEECHHCCCCCCCCHHHHHHHH
GLVMRDGIERLRNAKGYSGMYATYCTPLSWAGYYAGVGRLTGVDPREMRHSDLIVVWGGN
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHCCCCCCCCCHHHHCCCCEEEEECCC
PVNTQVNVMTHVGIARKTRGAKLACVDVYDTGTMKQADIKLLIRPGTDGALACAVMHVLF
CCCCEEEEEEECCCCCCCCCCEEEEEEEECCCCCEECCEEEEEECCCCHHHHHHHHHHHH
RDGYADREYLHRYTDCPDAFERHLDDKTPQWASRICDIPATEIEAFAALIGATPRSFFRI
HCCCCCHHHHHHHCCCHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHEE
GYGFTRSRNGAVNIHAVSSIPAVTGAWRHQGGGALHTNSGLYKWDKTLIEGRDAGVAPAR
CCCEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCCCCHHH
MLDQSRIGAVLLGDPHDLQGGPPVKAMLIQNTNPASVAPDQTSVKRGLARDDLFVCVHEQ
HHHHHHCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEHHH
FMTETARYADVVLPATMFLEHDDVYQGGGHQHILLGRRLVPPAGECRSNHEVISAIADRV
HHHHHHHHHHHHCCHHHEEECCCHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
GATHRGFAMTPRDIIEATLAASHRPGLDRLEVDGWLDVQTDFATAHNLDGFGHPDGKYHF
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCEEEEEECHHHHCCCCCCCCCCCCEEC
KPDWQQTAAYLGTRTLGPWEALPPFPDHCSLIEDATDEYPFRLATSPARTFLNSTFNETA
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHH
TSKTREGRPTVMVHPADLTALGLTSGDKVKLGSARGEVTLHCRANEGQRRGVLIAESIWP
CCCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCCEEEEEEECCCCCCCCEEEEECCCC
NDAHEDGCGINTLTGADQSAPAGGGSFHDNRVWIRKA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MNQTRANITIGRTVCPHDCPSACALDVELLDDDRIGRIRGARDNSYTLGVICEKVARYAE
CCCCCCEEEECCEECCCCCCCHHEEEEEEECCCCCCCEECCCCCCEEHHHHHHHHHHHHH
RFHHPDRLLHPLKRRGPKGTGDFVRISWEEALDLTATAFTTAERDHGAESVWPFYFAGTM
HHCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHCEEHHHEEECHHCCCCCCCCHHHHHHHH
GLVMRDGIERLRNAKGYSGMYATYCTPLSWAGYYAGVGRLTGVDPREMRHSDLIVVWGGN
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHCCCCCCCCCHHHHCCCCEEEEECCC
PVNTQVNVMTHVGIARKTRGAKLACVDVYDTGTMKQADIKLLIRPGTDGALACAVMHVLF
CCCCEEEEEEECCCCCCCCCCEEEEEEEECCCCCEECCEEEEEECCCCHHHHHHHHHHHH
RDGYADREYLHRYTDCPDAFERHLDDKTPQWASRICDIPATEIEAFAALIGATPRSFFRI
HCCCCCHHHHHHHCCCHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHEE
GYGFTRSRNGAVNIHAVSSIPAVTGAWRHQGGGALHTNSGLYKWDKTLIEGRDAGVAPAR
CCCEECCCCCEEEEEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHCCCCCCCCHHH
MLDQSRIGAVLLGDPHDLQGGPPVKAMLIQNTNPASVAPDQTSVKRGLARDDLFVCVHEQ
HHHHHHCCEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHCCCCCCEEEEEHHH
FMTETARYADVVLPATMFLEHDDVYQGGGHQHILLGRRLVPPAGECRSNHEVISAIADRV
HHHHHHHHHHHHCCHHHEEECCCHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHH
GATHRGFAMTPRDIIEATLAASHRPGLDRLEVDGWLDVQTDFATAHNLDGFGHPDGKYHF
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCEEEEEECHHHHCCCCCCCCCCCCEEC
KPDWQQTAAYLGTRTLGPWEALPPFPDHCSLIEDATDEYPFRLATSPARTFLNSTFNETA
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHH
TSKTREGRPTVMVHPADLTALGLTSGDKVKLGSARGEVTLHCRANEGQRRGVLIAESIWP
CCCCCCCCCEEEEECCCEEEEECCCCCEEEECCCCCEEEEEEECCCCCCCCEEEEECCCC
NDAHEDGCGINTLTGADQSAPAGGGSFHDNRVWIRKA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]