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The map label for this gene is cobQ [H]

Identifier: 86750396

GI number: 86750396

Start: 3757416

End: 3758993

Strand: Direct

Name: cobQ [H]

Synonym: RPB_3285

Alternate gene names: 86750396

Gene position: 3757416-3758993 (Clockwise)

Preceding gene: 86750394

Following gene: 86750413

Centisome position: 70.47

GC content: 69.96

Gene sequence:

>1578_bases
ATGACCACACCCGACGTCGATGAGTTGATCTCCCGCATCCATTTCACGCCGCGCGCGACCGCAGGTCCGCGGCGCGCCCG
CGCGCTGATGATCCAGGGCACCAGCAGCGACGTCGGCAAGAGCATGGTGGTCGCCGGCCTGTGCCGCGCCTACAGCCGCC
GCGGGCTCGTGGTGCGTCCGTTCAAGGCGCAGAACATGAGCAACAACGCGGCGGTCGCCGACGATCCCGATCTGCCGCCC
GGCCCCGACGGCGAGGCCGCGCGCGGCGAGATCGGCCGCGCCCAGGCGCTGCAGGCGCGCGCGTGCCGCACGACGCCGTC
GATCCACATGAACCCGGTGCTGCTCAAGCCGCAGACCGAGATCGGCGCGCAGGTGGTGCTGCGCGGCCGCGTGCTCGGCA
ATTGTCCGGCGCGGATCTATCACCACATGCGGCAGCGGCTGATCCCCGCGGTGCTCGACAGTTTCGAGCGGCTGGCCGAC
GAGGCCGACCTCGTGCTGGTGGAAGGCGCCGGCAGCGGCGCCGAGGTGTATCTGCGCGCCTCCGACATCACCAATATGCG
TTTCGCCGAAGCCGCCGATCTGCCAGTCGTGTTCCTGTCCGACATCGACCGCGGCGGCACGATGGCGGCGCTGGTCGGCA
GCTACGTGCTGCTGAACGACGGCGATCGCGCCCGCGTCGCCGGCTATCTGATCAACAAATTCCGCGGCGACTTCTCACTG
TTCGAGCCCGGCTGCCGCACCATCACCGAGCAGACCGGCTGGCCGTTTCTCGGCGTGCTGCGCTGGTTTCCGGGCGCCGA
CCGATTGCCGGCGGAAGACTCGCTGGCGCTGGAGCGGGCGGCGGCCGCGAGCCGCGGCCGGATCAGGATCGCGGTGCCGC
GGCTGCAGCGCGTGGCGAATTTCGACGATCTCGATCCGCTGGCGGCCGAGCCCGACGTCGATCTGCGCTGGATCCAGCCC
GGCTCGCCGATCCCCGGCGACGTCGACGTCGTGGTGCTGCCCGGCTCGAAAGCGACGCGCACCGAACTCGATCTGATCCG
GCGCGAAGGCTGGGATATCGATATCCTGGCGCATGTCCGCCGCGGCGGTCGCGTCGTCGGCCTGTGCGCGGGCTTCCAGA
TGCTGGGACGGGTCGTCCGCGACCCGAGGGGCATCGAGGGACCGCCCGGTGAAACGCCTGGCTTGGGTCTGCTCGATGTC
GAGACGGAAATTTCGGACGACAAGCGGCTGATCGAAATCGAAGCCGTCGACCTCAACGGCGGCTGTCGCGTCACCGGTTA
CGAGATGCACATGGGCCGGACTACCGGAGCCGGTCTGGCGCGGCCGTGGTTGCTGCTCGACGACGGGCGCGACGAATCAC
GACCGGAGGGTGCGGTGTCGGCGGACGGCCGCGTCATGGGCGCCTATCTGCACGGCCTGTTCGTCGGCGACGCGTTTCGC
TCGGCCTGGCTCGGCGAACTCGGTGCGGCGAGGTCCGGCCTCGACTTCGAGCGCCGCATCGAAGACGCGCTCGACGCGCT
CGCCGACCATTGCGAAGACAATCTCGATCTCGACGCGCTGCTGGCGCTGGCGCGCTAG

Upstream 100 bases:

>100_bases
CCGGCGTCGTATCGCCGACGCGGCGTCTGCGTCAAGCCGGCTTCGCGTTGCATCGCCATTCGTCCGCCCGCTCCTGCTTC
CCCTCCTATCGAGTTTCCCG

Downstream 100 bases:

>100_bases
CGACGGAGGATTGCCGCGTAGCGCGCCGATGCCACGCGGCGCCTGCGATCGATCGATGACCTTGCGCGACGCGTCCGCGC
CGGCGATTCAGAGATGCATC

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTTPDVDELISRIHFTPRATAGPRRARALMIQGTSSDVGKSMVVAGLCRAYSRRGLVVRPFKAQNMSNNAAVADDPDLPP
GPDGEAARGEIGRAQALQARACRTTPSIHMNPVLLKPQTEIGAQVVLRGRVLGNCPARIYHHMRQRLIPAVLDSFERLAD
EADLVLVEGAGSGAEVYLRASDITNMRFAEAADLPVVFLSDIDRGGTMAALVGSYVLLNDGDRARVAGYLINKFRGDFSL
FEPGCRTITEQTGWPFLGVLRWFPGADRLPAEDSLALERAAAASRGRIRIAVPRLQRVANFDDLDPLAAEPDVDLRWIQP
GSPIPGDVDVVVLPGSKATRTELDLIRREGWDIDILAHVRRGGRVVGLCAGFQMLGRVVRDPRGIEGPPGETPGLGLLDV
ETEISDDKRLIEIEAVDLNGGCRVTGYEMHMGRTTGAGLARPWLLLDDGRDESRPEGAVSADGRVMGAYLHGLFVGDAFR
SAWLGELGAARSGLDFERRIEDALDALADHCEDNLDLDALLALAR

Sequences:

>Translated_525_residues
MTTPDVDELISRIHFTPRATAGPRRARALMIQGTSSDVGKSMVVAGLCRAYSRRGLVVRPFKAQNMSNNAAVADDPDLPP
GPDGEAARGEIGRAQALQARACRTTPSIHMNPVLLKPQTEIGAQVVLRGRVLGNCPARIYHHMRQRLIPAVLDSFERLAD
EADLVLVEGAGSGAEVYLRASDITNMRFAEAADLPVVFLSDIDRGGTMAALVGSYVLLNDGDRARVAGYLINKFRGDFSL
FEPGCRTITEQTGWPFLGVLRWFPGADRLPAEDSLALERAAAASRGRIRIAVPRLQRVANFDDLDPLAAEPDVDLRWIQP
GSPIPGDVDVVVLPGSKATRTELDLIRREGWDIDILAHVRRGGRVVGLCAGFQMLGRVVRDPRGIEGPPGETPGLGLLDV
ETEISDDKRLIEIEAVDLNGGCRVTGYEMHMGRTTGAGLARPWLLLDDGRDESRPEGAVSADGRVMGAYLHGLFVGDAFR
SAWLGELGAARSGLDFERRIEDALDALADHCEDNLDLDALLALAR
>Mature_524_residues
TTPDVDELISRIHFTPRATAGPRRARALMIQGTSSDVGKSMVVAGLCRAYSRRGLVVRPFKAQNMSNNAAVADDPDLPPG
PDGEAARGEIGRAQALQARACRTTPSIHMNPVLLKPQTEIGAQVVLRGRVLGNCPARIYHHMRQRLIPAVLDSFERLADE
ADLVLVEGAGSGAEVYLRASDITNMRFAEAADLPVVFLSDIDRGGTMAALVGSYVLLNDGDRARVAGYLINKFRGDFSLF
EPGCRTITEQTGWPFLGVLRWFPGADRLPAEDSLALERAAAASRGRIRIAVPRLQRVANFDDLDPLAAEPDVDLRWIQPG
SPIPGDVDVVVLPGSKATRTELDLIRREGWDIDILAHVRRGGRVVGLCAGFQMLGRVVRDPRGIEGPPGETPGLGLLDVE
TEISDDKRLIEIEAVDLNGGCRVTGYEMHMGRTTGAGLARPWLLLDDGRDESRPEGAVSADGRVMGAYLHGLFVGDAFRS
AWLGELGAARSGLDFERRIEDALDALADHCEDNLDLDALLALAR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 56778; Mature: 56647

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPDVDELISRIHFTPRATAGPRRARALMIQGTSSDVGKSMVVAGLCRAYSRRGLVVRP
CCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEC
FKAQNMSNNAAVADDPDLPPGPDGEAARGEIGRAQALQARACRTTPSIHMNPVLLKPQTE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHH
IGAQVVLRGRVLGNCPARIYHHMRQRLIPAVLDSFERLADEADLVLVEGAGSGAEVYLRA
HCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEE
SDITNMRFAEAADLPVVFLSDIDRGGTMAALVGSYVLLNDGDRARVAGYLINKFRGDFSL
CCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCC
FEPGCRTITEQTGWPFLGVLRWFPGADRLPAEDSLALERAAAASRGRIRIAVPRLQRVAN
CCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHCC
FDDLDPLAAEPDVDLRWIQPGSPIPGDVDVVVLPGSKATRTELDLIRREGWDIDILAHVR
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEHHHHH
RGGRVVGLCAGFQMLGRVVRDPRGIEGPPGETPGLGLLDVETEISDDKRLIEIEAVDLNG
CCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCC
GCRVTGYEMHMGRTTGAGLARPWLLLDDGRDESRPEGAVSADGRVMGAYLHGLFVGDAFR
CEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
SAWLGELGAARSGLDFERRIEDALDALADHCEDNLDLDALLALAR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TTPDVDELISRIHFTPRATAGPRRARALMIQGTSSDVGKSMVVAGLCRAYSRRGLVVRP
CCCCHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEC
FKAQNMSNNAAVADDPDLPPGPDGEAARGEIGRAQALQARACRTTPSIHMNPVLLKPQTE
HHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCHH
IGAQVVLRGRVLGNCPARIYHHMRQRLIPAVLDSFERLADEADLVLVEGAGSGAEVYLRA
HCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEE
SDITNMRFAEAADLPVVFLSDIDRGGTMAALVGSYVLLNDGDRARVAGYLINKFRGDFSL
CCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHCCEEEECCCCCHHHHHHHHHHHCCCCCC
FEPGCRTITEQTGWPFLGVLRWFPGADRLPAEDSLALERAAAASRGRIRIAVPRLQRVAN
CCCCHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHCC
FDDLDPLAAEPDVDLRWIQPGSPIPGDVDVVVLPGSKATRTELDLIRREGWDIDILAHVR
CCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEHHHHH
RGGRVVGLCAGFQMLGRVVRDPRGIEGPPGETPGLGLLDVETEISDDKRLIEIEAVDLNG
CCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCC
GCRVTGYEMHMGRTTGAGLARPWLLLDDGRDESRPEGAVSADGRVMGAYLHGLFVGDAFR
CEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
SAWLGELGAARSGLDFERRIEDALDALADHCEDNLDLDALLALAR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]