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The map label for this gene is cobS [H]

Identifier: 86750394

GI number: 86750394

Start: 3756014

End: 3756790

Strand: Direct

Name: cobS [H]

Synonym: RPB_3283

Alternate gene names: 86750394

Gene position: 3756014-3756790 (Clockwise)

Preceding gene: 86750393

Following gene: 86750396

Centisome position: 70.45

GC content: 69.24

Gene sequence:

>777_bases
GTGACCGAACGCATCCGGCCGCTGCTGAACGCGATTCAGTTCATGACGGTGTTGCCGACGCGGGGAGCCGCGCGCCTCGA
CGCCGATTGGGTGTTGTCCGCAGTCAAGTACTTCCCCCTCGTCGGCGCAATGATCGGCTTCGTCTGCGCCGGCGTGTTGC
TGATCGCCTCGACGATCTGGACCGGGACTGTGGCCGCCACGCTGGCGATTCTCGCGGGCGTGATCCTCACCGGCGCATTG
CACGAAGACGGCGTCGCCGACACCGCCGACGGCTTGTTCGGTGGCCGCACGCGCGAGCAGCGTCTGCTGATCATCAAGGA
CAGCCGGATCGGCAGCTACGGCGCGGTGACGCTGGTCGCGAGCCTGCTCCTCCGCATCGTGGTGCTCGCGAGCCTGCCCC
CCTGGCTCGGCGCAGCCGCATTGGTGGCCGGCCACAGCCTCGGTCGCGCCGGCATCGTGCTGGTGATGTCGGTGCTGCCC
TATGCTGGCAACGTCGCGACCGCCAAGCTGAACTACCCGACGCAGCGGATCGGCACCGCCGACGCCATCGTGGCCGCGGC
GTTCACGGCGCTGGGGATGATGTGGCTGGTGATCGGCGATGCCGGTGCGGCGGCCTGCGGGCTGGCGGGCGCCGTGATCG
CGGGGCTGCTACCTGCTCTGGCGGCGCATCGTCTGCTCGGCGGCTATGTCGGCGACGTACTCGGCGCGACCGAACAACTC
GCCCAGATCGGACTGTTGCTCGGCGTCGCGGCGATTGCAGGCGGGAGTCAGATCTGA

Upstream 100 bases:

>100_bases
GCGCGCTGATGGCGATTCCGCTGGTGCGCGCCGCCGCCGGAATCCTCACCGACTTCGCCGACCTCTCCGACGTTCTGGCC
GGCACCATCGGCACGCCGTC

Downstream 100 bases:

>100_bases
TGCGCCGGACCATCGCGCCAGTCGAGCCGCAAGCAGAACGGCGCGGCGTCGCCGTCATACTCGACTCCCCGCCCGATGTG
CTCGATCGCCTCGACCATGC

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTERIRPLLNAIQFMTVLPTRGAARLDADWVLSAVKYFPLVGAMIGFVCAGVLLIASTIWTGTVAATLAILAGVILTGAL
HEDGVADTADGLFGGRTREQRLLIIKDSRIGSYGAVTLVASLLLRIVVLASLPPWLGAAALVAGHSLGRAGIVLVMSVLP
YAGNVATAKLNYPTQRIGTADAIVAAAFTALGMMWLVIGDAGAAACGLAGAVIAGLLPALAAHRLLGGYVGDVLGATEQL
AQIGLLLGVAAIAGGSQI

Sequences:

>Translated_258_residues
MTERIRPLLNAIQFMTVLPTRGAARLDADWVLSAVKYFPLVGAMIGFVCAGVLLIASTIWTGTVAATLAILAGVILTGAL
HEDGVADTADGLFGGRTREQRLLIIKDSRIGSYGAVTLVASLLLRIVVLASLPPWLGAAALVAGHSLGRAGIVLVMSVLP
YAGNVATAKLNYPTQRIGTADAIVAAAFTALGMMWLVIGDAGAAACGLAGAVIAGLLPALAAHRLLGGYVGDVLGATEQL
AQIGLLLGVAAIAGGSQI
>Mature_257_residues
TERIRPLLNAIQFMTVLPTRGAARLDADWVLSAVKYFPLVGAMIGFVCAGVLLIASTIWTGTVAATLAILAGVILTGALH
EDGVADTADGLFGGRTREQRLLIIKDSRIGSYGAVTLVASLLLRIVVLASLPPWLGAAALVAGHSLGRAGIVLVMSVLPY
AGNVATAKLNYPTQRIGTADAIVAAAFTALGMMWLVIGDAGAAACGLAGAVIAGLLPALAAHRLLGGYVGDVLGATEQLA
QIGLLLGVAAIAGGSQI

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=32.1138211382114, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26099; Mature: 25968

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERIRPLLNAIQFMTVLPTRGAARLDADWVLSAVKYFPLVGAMIGFVCAGVLLIASTIW
CCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGTVAATLAILAGVILTGALHEDGVADTADGLFGGRTREQRLLIIKDSRIGSYGAVTLVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
SLLLRIVVLASLPPWLGAAALVAGHSLGRAGIVLVMSVLPYAGNVATAKLNYPTQRIGTA
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHCHH
DAIVAAAFTALGMMWLVIGDAGAAACGLAGAVIAGLLPALAAHRLLGGYVGDVLGATEQL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQIGLLLGVAAIAGGSQI
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TERIRPLLNAIQFMTVLPTRGAARLDADWVLSAVKYFPLVGAMIGFVCAGVLLIASTIW
CHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGTVAATLAILAGVILTGALHEDGVADTADGLFGGRTREQRLLIIKDSRIGSYGAVTLVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHH
SLLLRIVVLASLPPWLGAAALVAGHSLGRAGIVLVMSVLPYAGNVATAKLNYPTQRIGTA
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHCHH
DAIVAAAFTALGMMWLVIGDAGAAACGLAGAVIAGLLPALAAHRLLGGYVGDVLGATEQL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQIGLLLGVAAIAGGSQI
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA