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The map label for this gene is proS

Identifier: 86749715

GI number: 86749715

Start: 2978821

End: 2980140

Strand: Direct

Name: proS

Synonym: RPB_2597

Alternate gene names: 86749715

Gene position: 2978821-2980140 (Clockwise)

Preceding gene: 86749714

Following gene: 86749716

Centisome position: 55.87

GC content: 64.92

Gene sequence:

>1320_bases
ATGCGTTTGTCGCGATTCTTTCTGCCGATCCTGAAGGAAAACCCGAAGGAAGCCGAGATCGTCTCGCATCGGCTGATGCT
GCGCGCCGGGATGCTGCGGCAGGAGGCCGCCGGCATCTATGCCTGGCTGCCGCTCGGCCATCGGGTGCTGAAGAAGATCG
AGCAGATCGTGCGAGAGGAGCAGAACCGCGCCGGCGCGATCGAGCTGTTGATGCCGACGCTGCAACTCGCCGATCTGTGG
CGCGAGAGCGGCCGCTACGACGCCTATGGTCCGGAGATGCTGCGGATCAGCGACCGCCACAAGCGCGAGCTGCTGTACGG
GCCGACCAACGAGGAAATGATCACCGAGATCTTCCGCGCCTATGTGAAGTCGTACAAGAGCCTGCCGCTCAACCTCTATC
ACATCCAGTGGAAGTTCCGCGACGAGCAGCGTCCGCGCTTCGGCGTGATGCGCGGCCGCGAGTTCCTGATGAAGGACGCC
TATTCGTTCGACGTCGATGAAGCCGCCGCGCGCAAGTCGTACAACCGGATGTTCGTCGCTTACTTGCGGACCTTCGCGCG
GATGGGGCTGAAGGCGATCCCGATGCGCGCCGAGACCGGACCGATCGGCGGCGACCTCAGCCACGAGTTCATCGTGCTGG
CCGAGACCGGCGAGTCCGGCGTGTATTGCGACCGCGACGTGCTGAGCCTGCCGGTGCCGGACGAGACCGTCGATTATGAC
GGAGATCTGACGCCGATCATCAAGCAATGGACATCGGTCTACGCCGCGACCGAGGACGTCCACGACGCCGTGCGCTACGA
GAGCGAAGTGCCGGAAGCCAACCGGCTGCACACCCGCGGCATCGAGGTCGGCCAGATCTTCTATTTCGGCACCAAATATT
CCGACTCGATGAAGGCCAATGTCGCCGGTCCCGACGGCACCGATGCGCCGATCCACGGCGGCTCCTACGGCGTCGGCGTG
TCGCGCCTCGTCGGCGCGATCATCGAGGCGTGCCACGACGACAACGGCATCATCTGGCCGGAGGAGGTGGCGCCGTTCCG
GGTCGCAATCCTGAACCTCAAGCAGGGTGACGCCGCGACCGATGCGGCCTGCGAACAGCTCTACAAGGAGCTCGCCGCCA
AGGGCGTCGACGTGCTGTACGACGACACCGATCAGCGCGCCGGCGCGAAATTCGCGACTGCGGATCTGATCGGAATTCCG
TGGCAGGTGCTGGTCGGGCCGAAGGGCCTCGCCGACGGCAAGATCGAACTGAAGCGGCGCAGTGACGGCTCGCGCGAGAA
TATCGCGCTGGCGGAGACGGTCGCACGGCTCGCGCCGTAA

Upstream 100 bases:

>100_bases
GCAGTTTTCGCAAAGTTGGGCGGCCGCGCCCTTGTGTTTTGCCGCCCGATCCGGTTTCACTGGCGCAAACCTGCTTCCCC
CTGATTCTTGAGCAAATCCC

Downstream 100 bases:

>100_bases
GATTTATCCACTGGCGGCTCGGCTGAAATTAGCCCGAATCGTGTGAAATTCGGAGCATGGATCACAGCATGACTGAGCCA
GTTCGAACTCCTCCTTTTGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGHRVLKKIEQIVREEQNRAGAIELLMPTLQLADLW
RESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRAYVKSYKSLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDVDEAAARKSYNRMFVAYLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRDVLSLPVPDETVDYD
GDLTPIIKQWTSVYAATEDVHDAVRYESEVPEANRLHTRGIEVGQIFYFGTKYSDSMKANVAGPDGTDAPIHGGSYGVGV
SRLVGAIIEACHDDNGIIWPEEVAPFRVAILNLKQGDAATDAACEQLYKELAAKGVDVLYDDTDQRAGAKFATADLIGIP
WQVLVGPKGLADGKIELKRRSDGSRENIALAETVARLAP

Sequences:

>Translated_439_residues
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGHRVLKKIEQIVREEQNRAGAIELLMPTLQLADLW
RESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRAYVKSYKSLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDVDEAAARKSYNRMFVAYLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRDVLSLPVPDETVDYD
GDLTPIIKQWTSVYAATEDVHDAVRYESEVPEANRLHTRGIEVGQIFYFGTKYSDSMKANVAGPDGTDAPIHGGSYGVGV
SRLVGAIIEACHDDNGIIWPEEVAPFRVAILNLKQGDAATDAACEQLYKELAAKGVDVLYDDTDQRAGAKFATADLIGIP
WQVLVGPKGLADGKIELKRRSDGSRENIALAETVARLAP
>Mature_439_residues
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGHRVLKKIEQIVREEQNRAGAIELLMPTLQLADLW
RESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRAYVKSYKSLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDA
YSFDVDEAAARKSYNRMFVAYLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRDVLSLPVPDETVDYD
GDLTPIIKQWTSVYAATEDVHDAVRYESEVPEANRLHTRGIEVGQIFYFGTKYSDSMKANVAGPDGTDAPIHGGSYGVGV
SRLVGAIIEACHDDNGIIWPEEVAPFRVAILNLKQGDAATDAACEQLYKELAAKGVDVLYDDTDQRAGAKFATADLIGIP
WQVLVGPKGLADGKIELKRRSDGSRENIALAETVARLAP

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=411, Percent_Identity=32.8467153284672, Blast_Score=235, Evalue=5e-62,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=59.2760180995475, Blast_Score=281, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI115532348, Length=403, Percent_Identity=29.7766749379653, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=33.7078651685393, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320931, Length=267, Percent_Identity=32.2097378277154, Blast_Score=149, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24656200, Length=457, Percent_Identity=29.3216630196937, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_RHOP2 (Q2IWW0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486211.1
- ProteinModelPortal:   Q2IWW0
- SMR:   Q2IWW0
- STRING:   Q2IWW0
- GeneID:   3910389
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2597
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK12325
- BioCyc:   RPAL316058:RPB_2597-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49302; Mature: 49302

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGHRVLKKIEQIVREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHCCHHHHHHHHHHHHHH
QNRAGAIELLMPTLQLADLWRESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRA
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHEEECCCCHHHHHHHHHH
YVKSYKSLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDVDEAAARKSYNRMFVA
HHHHHHCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
YLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRDVLSLPVPDETVDYD
HHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCCEEECCCCCCCCCCC
GDLTPIIKQWTSVYAATEDVHDAVRYESEVPEANRLHTRGIEVGQIFYFGTKYSDSMKAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEE
VAGPDGTDAPIHGGSYGVGVSRLVGAIIEACHDDNGIIWPEEVAPFRVAILNLKQGDAAT
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHH
DAACEQLYKELAAKGVDVLYDDTDQRAGAKFATADLIGIPWQVLVGPKGLADGKIELKRR
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEHHHHCCCCEEEEECCCCCCCCEEEEEEC
SDGSRENIALAETVARLAP
CCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGHRVLKKIEQIVREE
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEHHCCHHHHHHHHHHHHHH
QNRAGAIELLMPTLQLADLWRESGRYDAYGPEMLRISDRHKRELLYGPTNEEMITEIFRA
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHEEECCCCHHHHHHHHHH
YVKSYKSLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDVDEAAARKSYNRMFVA
HHHHHHCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
YLRTFARMGLKAIPMRAETGPIGGDLSHEFIVLAETGESGVYCDRDVLSLPVPDETVDYD
HHHHHHHCCCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCCEEECCCCCCCCCCC
GDLTPIIKQWTSVYAATEDVHDAVRYESEVPEANRLHTRGIEVGQIFYFGTKYSDSMKAN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEE
VAGPDGTDAPIHGGSYGVGVSRLVGAIIEACHDDNGIIWPEEVAPFRVAILNLKQGDAAT
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHH
DAACEQLYKELAAKGVDVLYDDTDQRAGAKFATADLIGIPWQVLVGPKGLADGKIELKRR
HHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEHHHHCCCCEEEEECCCCCCCCEEEEEEC
SDGSRENIALAETVARLAP
CCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA