Click here to switch to the map view.

The map label for this gene is nuoN [H]

Identifier: 86749705

GI number: 86749705

Start: 2968914

End: 2970350

Strand: Direct

Name: nuoN [H]

Synonym: RPB_2587

Alternate gene names: 86749705

Gene position: 2968914-2970350 (Clockwise)

Preceding gene: 86749704

Following gene: 86749706

Centisome position: 55.68

GC content: 63.6

Gene sequence:

>1437_bases
ATGAATTTCGAAACTGCCGGTTATTCGCTGTTGCCGATCATGCCGGAGCTCGTGCTGGTGATCGGCTCGATGCTGCTGCT
GATGGTCGGCGCCTATGGTGGCCAACGCACGACCGGCCTCGTTACGACCCTCGCGGTCGCGTTGCTGGTGCTGACCGGTG
CGCTGGAATTGATGCTGCCGGCGGGCAGGCTCGTGACCTTCGGCGGCAGCTTCATCGTCGACGATTTCGCCCGCTTCCTG
AAGGTACTGGCGCTGATCGGATCGGCTGTGACGCTGATCCTGTCGTCCGAGATCATGTCGAATCCGTCGCGGCGGATGTT
CGAATATTCGATCCTGGTGCTGCTGTCGTCGGTCGGCATGATGCTGCTGATCTCGGCCGGCGACCTTATCATGCTGTATC
TCGGCCTCGAGCTGATGAGTCTGGCGCTCTACGTGGTGGCGGCGAGCAATCGCGAGGACGCCAAGTCGAACGAAGCCGGC
ATGAAGTATTTCATCCTCGGCGCGTTGTCGTCAGGCATGCTGCTGTACGGCTGCTCGCTGATCTACGGCTTCACCGGCAC
GGTGCAGTTCGCCGGCATCGCCGCCGCCGCCAAGGACGGCAGTATCGGCCTGATCTTCGGCCTGGTGTTCCTGCTCGCCG
GGCTGTGCTTCAAGGTCTCTGCGGTGCCGTTCCACATGTGGACGCCGGACGTCTACGAGGGCGCCCCGACCCCGGTGACG
GCGTTCTTCGCCTCCGCCCCGAAGGTGGCGGGTCTGGCGGTGTTCACTCGCGTGACGCTGACGGCGTTTCCGGAGATCGT
GCCGCAGTGGCAGCAGATCGTGGTGTTCGTCGCGATCGCCTCGATGGCGCTGGGTTCGTTCGCCGCGATCGGGCAGAAGA
ACATCAAGCGCCTGATGGCGTATTCGGCGATCGGCCATATCGGTTTCGCGCTGGTCGGCCTGTCGGCCGGCACCGTCGAG
GGCGCGCAGGGCGTGATCGTCTACATCTCGATCTACGTGGCGATGACACTCGGCGCGTTCTCGGTGATCCTGGCGATGCG
CCGCAATGGCTTGCATGTCGAGAACATCAGCGATTTCGCCGGCCTGTCGCGCACCAATCCGGCGCTGGCGTTCTTCTTCG
CGATGCTGCTGTTCTCGATGGCGGGCATCCCGCCGCTCGCCGGCTTCTTCGCCAAATTCTACGTGTTCATGGCGGCGATC
AAGGCCGGGCTGTTCGCGCTGGCGGTGATCGGCGTCGTCACCAGCGTGGTCGGCGCCTACTATTATCTGCTGATCATCAA
GGTAATGTATTTCGACGAGCCGGCGCCGGGGATCGACCGGATGCGGCTCGAGCTGCGCGGCGTGCTCGCGGTCGCCGGGC
TTTTCAACATGTTGTTCTTCCTCTATCCGGCGCCGCTGGTGAACGCCGCGAGCGCCGCAGCCAAGTCGCTGTTCTAG

Upstream 100 bases:

>100_bases
ATGTCGGCCGCCTCGGTCCAGCAACTCGTCAACAATTACGCCGCCGCCGTGACTGCCGTGAAGGCAGCCGCGCTTCCGTA
ACCGGCAAGGACCGCAAGCT

Downstream 100 bases:

>100_bases
GTGGCGTTCACCCTCGGTCCGAAGGCGCGCGCGGCCGGCGTCGGCCTGAAGGTTTTCGACGAGACCGGCTCCACCAACGC
CGACGCAATGGCATATGCCC

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNFETAGYSLLPIMPELVLVIGSMLLLMVGAYGGQRTTGLVTTLAVALLVLTGALELMLPAGRLVTFGGSFIVDDFARFL
KVLALIGSAVTLILSSEIMSNPSRRMFEYSILVLLSSVGMMLLISAGDLIMLYLGLELMSLALYVVAASNREDAKSNEAG
MKYFILGALSSGMLLYGCSLIYGFTGTVQFAGIAAAAKDGSIGLIFGLVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVT
AFFASAPKVAGLAVFTRVTLTAFPEIVPQWQQIVVFVAIASMALGSFAAIGQKNIKRLMAYSAIGHIGFALVGLSAGTVE
GAQGVIVYISIYVAMTLGAFSVILAMRRNGLHVENISDFAGLSRTNPALAFFFAMLLFSMAGIPPLAGFFAKFYVFMAAI
KAGLFALAVIGVVTSVVGAYYYLLIIKVMYFDEPAPGIDRMRLELRGVLAVAGLFNMLFFLYPAPLVNAASAAAKSLF

Sequences:

>Translated_478_residues
MNFETAGYSLLPIMPELVLVIGSMLLLMVGAYGGQRTTGLVTTLAVALLVLTGALELMLPAGRLVTFGGSFIVDDFARFL
KVLALIGSAVTLILSSEIMSNPSRRMFEYSILVLLSSVGMMLLISAGDLIMLYLGLELMSLALYVVAASNREDAKSNEAG
MKYFILGALSSGMLLYGCSLIYGFTGTVQFAGIAAAAKDGSIGLIFGLVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVT
AFFASAPKVAGLAVFTRVTLTAFPEIVPQWQQIVVFVAIASMALGSFAAIGQKNIKRLMAYSAIGHIGFALVGLSAGTVE
GAQGVIVYISIYVAMTLGAFSVILAMRRNGLHVENISDFAGLSRTNPALAFFFAMLLFSMAGIPPLAGFFAKFYVFMAAI
KAGLFALAVIGVVTSVVGAYYYLLIIKVMYFDEPAPGIDRMRLELRGVLAVAGLFNMLFFLYPAPLVNAASAAAKSLF
>Mature_478_residues
MNFETAGYSLLPIMPELVLVIGSMLLLMVGAYGGQRTTGLVTTLAVALLVLTGALELMLPAGRLVTFGGSFIVDDFARFL
KVLALIGSAVTLILSSEIMSNPSRRMFEYSILVLLSSVGMMLLISAGDLIMLYLGLELMSLALYVVAASNREDAKSNEAG
MKYFILGALSSGMLLYGCSLIYGFTGTVQFAGIAAAAKDGSIGLIFGLVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVT
AFFASAPKVAGLAVFTRVTLTAFPEIVPQWQQIVVFVAIASMALGSFAAIGQKNIKRLMAYSAIGHIGFALVGLSAGTVE
GAQGVIVYISIYVAMTLGAFSVILAMRRNGLHVENISDFAGLSRTNPALAFFFAMLLFSMAGIPPLAGFFAKFYVFMAAI
KAGLFALAVIGVVTSVVGAYYYLLIIKVMYFDEPAPGIDRMRLELRGVLAVAGLFNMLFFLYPAPLVNAASAAAKSLF

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=409, Percent_Identity=34.718826405868, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1788614, Length=338, Percent_Identity=28.1065088757396, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788613, Length=511, Percent_Identity=24.4618395303327, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788827, Length=320, Percent_Identity=26.5625, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788831, Length=296, Percent_Identity=26.0135135135135, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788829, Length=267, Percent_Identity=25.0936329588015, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50678; Mature: 50678

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFETAGYSLLPIMPELVLVIGSMLLLMVGAYGGQRTTGLVTTLAVALLVLTGALELMLP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
AGRLVTFGGSFIVDDFARFLKVLALIGSAVTLILSSEIMSNPSRRMFEYSILVLLSSVGM
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MLLISAGDLIMLYLGLELMSLALYVVAASNREDAKSNEAGMKYFILGALSSGMLLYGCSL
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHH
IYGFTGTVQFAGIAAAAKDGSIGLIFGLVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVT
HHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCHHCCCCCCCHH
AFFASAPKVAGLAVFTRVTLTAFPEIVPQWQQIVVFVAIASMALGSFAAIGQKNIKRLMA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSAIGHIGFALVGLSAGTVEGAQGVIVYISIYVAMTLGAFSVILAMRRNGLHVENISDFA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GLSRTNPALAFFFAMLLFSMAGIPPLAGFFAKFYVFMAAIKAGLFALAVIGVVTSVVGAY
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YYLLIIKVMYFDEPAPGIDRMRLELRGVLAVAGLFNMLFFLYPAPLVNAASAAAKSLF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFETAGYSLLPIMPELVLVIGSMLLLMVGAYGGQRTTGLVTTLAVALLVLTGALELMLP
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
AGRLVTFGGSFIVDDFARFLKVLALIGSAVTLILSSEIMSNPSRRMFEYSILVLLSSVGM
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
MLLISAGDLIMLYLGLELMSLALYVVAASNREDAKSNEAGMKYFILGALSSGMLLYGCSL
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHCCHHHHHHHH
IYGFTGTVQFAGIAAAAKDGSIGLIFGLVFLLAGLCFKVSAVPFHMWTPDVYEGAPTPVT
HHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCHHCCCCCCCHH
AFFASAPKVAGLAVFTRVTLTAFPEIVPQWQQIVVFVAIASMALGSFAAIGQKNIKRLMA
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSAIGHIGFALVGLSAGTVEGAQGVIVYISIYVAMTLGAFSVILAMRRNGLHVENISDFA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GLSRTNPALAFFFAMLLFSMAGIPPLAGFFAKFYVFMAAIKAGLFALAVIGVVTSVVGAY
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YYLLIIKVMYFDEPAPGIDRMRLELRGVLAVAGLFNMLFFLYPAPLVNAASAAAKSLF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA