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The map label for this gene is birA [H]

Identifier: 86749706

GI number: 86749706

Start: 2970351

End: 2971166

Strand: Direct

Name: birA [H]

Synonym: RPB_2588

Alternate gene names: 86749706

Gene position: 2970351-2971166 (Clockwise)

Preceding gene: 86749705

Following gene: 86749707

Centisome position: 55.71

GC content: 64.95

Gene sequence:

>816_bases
GTGGCGTTCACCCTCGGTCCGAAGGCGCGCGCGGCCGGCGTCGGCCTGAAGGTTTTCGACGAGACCGGCTCCACCAACGC
CGACGCAATGGCATATGCCCGAGCGGGAGGGCGGACGCCTTGCTGGTTCGTGACGACAATGCAGACCGCGGGACGCGGTC
GTCGCAATCGGGTCTGGGTGGCTCCGCGCGGCAATTTGGCCAGCTCCGTCTTCGAGAGCTTCAATGTCACCCCGGCCGTC
GCCGCGACCCTCGGCTTTGCTGCAGGCGTCGCGCTCGAGAAGGCCTTGCGGGAGGTGAGCGTCGAGGCCGCAATGCGCTC
GCCCAATTCCGCTGCGAACGATTATCGGCTGAAGTGGCCCAACGATATTCTGGCAGGCGGATCGAAGCTGGTCGGGATGA
ATCTCGAAGCCGAGACGTTGCCGGACGGACGCCTCGCCGTTGTGGTCGGAATGGGCACGAACGTGGTCGCTGCGCCGGAA
GGTCTTCCGACCGCGGTGACGTGCTTGAGCCAGCTCGGCGTGTCCGCCAGCGCGGAAGACGTATTTGCGGCGCTGTCGGA
TTCGTGGCACGAGATGCGCGGCATCTGGGATGACGGGCGTGGTTTCGCCAAGATCCGCGATCTCTGGCTGGATCGGGCCG
CAGGGCTCGGTCAGACGGTTTCCATCCGGTCGGGCTCGTCGGTGGTTTCCGGGATATTCGACACCATCGATGAAACGGGC
TGTATGGTCCTGATAACGTCCGATCGGAGACGCATTCCGATCGCGGCCGGCGACGTCTATTTCGGCGATGCGGCGTCAGC
AAAGGCTGCAGACTAA

Upstream 100 bases:

>100_bases
CGGCGTGCTCGCGGTCGCCGGGCTTTTCAACATGTTGTTCTTCCTCTATCCGGCGCCGCTGGTGAACGCCGCGAGCGCCG
CAGCCAAGTCGCTGTTCTAG

Downstream 100 bases:

>100_bases
TGGCACGACCCGACGATCTGGTTTTTGCACCGCTTGGCGGTGTCGGCGAAATCGGCATGAATTTGTCGATCTACGGGCTC
GGCAACCGCCAGCAGCGGAG

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MAFTLGPKARAAGVGLKVFDETGSTNADAMAYARAGGRTPCWFVTTMQTAGRGRRNRVWVAPRGNLASSVFESFNVTPAV
AATLGFAAGVALEKALREVSVEAAMRSPNSAANDYRLKWPNDILAGGSKLVGMNLEAETLPDGRLAVVVGMGTNVVAAPE
GLPTAVTCLSQLGVSASAEDVFAALSDSWHEMRGIWDDGRGFAKIRDLWLDRAAGLGQTVSIRSGSSVVSGIFDTIDETG
CMVLITSDRRRIPIAAGDVYFGDAASAKAAD

Sequences:

>Translated_271_residues
MAFTLGPKARAAGVGLKVFDETGSTNADAMAYARAGGRTPCWFVTTMQTAGRGRRNRVWVAPRGNLASSVFESFNVTPAV
AATLGFAAGVALEKALREVSVEAAMRSPNSAANDYRLKWPNDILAGGSKLVGMNLEAETLPDGRLAVVVGMGTNVVAAPE
GLPTAVTCLSQLGVSASAEDVFAALSDSWHEMRGIWDDGRGFAKIRDLWLDRAAGLGQTVSIRSGSSVVSGIFDTIDETG
CMVLITSDRRRIPIAAGDVYFGDAASAKAAD
>Mature_270_residues
AFTLGPKARAAGVGLKVFDETGSTNADAMAYARAGGRTPCWFVTTMQTAGRGRRNRVWVAPRGNLASSVFESFNVTPAVA
ATLGFAAGVALEKALREVSVEAAMRSPNSAANDYRLKWPNDILAGGSKLVGMNLEAETLPDGRLAVVVGMGTNVVAAPEG
LPTAVTCLSQLGVSASAEDVFAALSDSWHEMRGIWDDGRGFAKIRDLWLDRAAGLGQTVSIRSGSSVVSGIFDTIDETGC
MVLITSDRRRIPIAAGDVYFGDAASAKAAD

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=235, Percent_Identity=25.9574468085106, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28304; Mature: 28173

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTLGPKARAAGVGLKVFDETGSTNADAMAYARAGGRTPCWFVTTMQTAGRGRRNRVWV
CCEECCCCCHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEEHHCCCCCCCEEEE
APRGNLASSVFESFNVTPAVAATLGFAAGVALEKALREVSVEAAMRSPNSAANDYRLKWP
ECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
NDILAGGSKLVGMNLEAETLPDGRLAVVVGMGTNVVAAPEGLPTAVTCLSQLGVSASAED
CHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCHHH
VFAALSDSWHEMRGIWDDGRGFAKIRDLWLDRAAGLGQTVSIRSGSSVVSGIFDTIDETG
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCC
CMVLITSDRRRIPIAAGDVYFGDAASAKAAD
EEEEEECCCCCCCEEECCEEECCCCCCCCCC
>Mature Secondary Structure 
AFTLGPKARAAGVGLKVFDETGSTNADAMAYARAGGRTPCWFVTTMQTAGRGRRNRVWV
CEECCCCCHHCCCCEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEEHHCCCCCCCEEEE
APRGNLASSVFESFNVTPAVAATLGFAAGVALEKALREVSVEAAMRSPNSAANDYRLKWP
ECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
NDILAGGSKLVGMNLEAETLPDGRLAVVVGMGTNVVAAPEGLPTAVTCLSQLGVSASAED
CHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCHHH
VFAALSDSWHEMRGIWDDGRGFAKIRDLWLDRAAGLGQTVSIRSGSSVVSGIFDTIDETG
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCC
CMVLITSDRRRIPIAAGDVYFGDAASAKAAD
EEEEEECCCCCCCEEECCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]