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The map label for this gene is lipB

Identifier: 86749491

GI number: 86749491

Start: 2725687

End: 2726454

Strand: Reverse

Name: lipB

Synonym: RPB_2371

Alternate gene names: 86749491

Gene position: 2726454-2725687 (Counterclockwise)

Preceding gene: 86749493

Following gene: 86749490

Centisome position: 51.14

GC content: 70.31

Gene sequence:

>768_bases
ATGATTAACGCGCCGCTTCATGCCCGTCAGACGCTCGATTTGGCTGGTTTTCGCCCTCAGGCCGACGCCCGGCCGGTGGA
ATGGCTGATCTCGGAAGCTCCGGTGCCCTACCCCGACGCGGTCGCGGCGATGGAATCACGCGCCGCGGCGATCGCCGCCG
GCGACGCCGCCGAGCTGGTCTGGCTGCTCGAACATCCGCCGCTCTACACCTCCGGCACCAGCGGCCAGTCCACCGACCTG
CTCGACCCGCGGTTTCCGCTGCACACCACCGGGCGCGGCGGCCAGCTCACCTATCACGGCCCCGGCCAGCGGGTGGCCTA
TGTGATGCTCGACCTCAAGCGCCGCCGCCCCGACGTCCGCGCCTATGTGGCCGCGCTGGAGCAGTGGATCATCGCGACGC
TCGACGCTTTCAATGTGCGCGGCGAGCGCCGCGAGAACCGCGTCGGCGTCTGGGTGGCGCGGCCCGACAAGGGCGCGGAC
CACGAGGACAAGATCGCCGCGATCGGCGTCCGGCTGAAGCGCTGGGTGTCGCTGCACGGCATCGCCATCAACGTCGAACC
CGATCTGAGCCACTTCACCGCCATCGTGCCCTGCGGCATCAGCGACCCGCGCTACGGCGTCACCAGCCTGGTCGATCTCG
GCCTGCCGGTGACGATGGCCGACGCCGACATCGCGCTGCGCGCGGCCTTCACCGAGATCTTCGGCGCGACCGTGGATGCG
CCGCAAGTCGATCCGGCTGTCGATGCGGGCGCGAGGTCGCCGGCTTGA

Upstream 100 bases:

>100_bases
CCATGAGATCGACCGAAACATTATCGAGTGTTGGCACTGGGACCCGGACCGGCAGGCTGTGAATGAAAGTGTGCGAACTT
GACCACGTTATGGTTATTCA

Downstream 100 bases:

>100_bases
ACACAGCGATCATCTATGTCGGCGCGGCGATCGCCGAGATTGCCGGCTGCTTCGCGTTCTGGGGCTGGCTGCGGCTCGGC
AAGCCGGTGTGGTGGCTGGC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MINAPLHARQTLDLAGFRPQADARPVEWLISEAPVPYPDAVAAMESRAAAIAAGDAAELVWLLEHPPLYTSGTSGQSTDL
LDPRFPLHTTGRGGQLTYHGPGQRVAYVMLDLKRRRPDVRAYVAALEQWIIATLDAFNVRGERRENRVGVWVARPDKGAD
HEDKIAAIGVRLKRWVSLHGIAINVEPDLSHFTAIVPCGISDPRYGVTSLVDLGLPVTMADADIALRAAFTEIFGATVDA
PQVDPAVDAGARSPA

Sequences:

>Translated_255_residues
MINAPLHARQTLDLAGFRPQADARPVEWLISEAPVPYPDAVAAMESRAAAIAAGDAAELVWLLEHPPLYTSGTSGQSTDL
LDPRFPLHTTGRGGQLTYHGPGQRVAYVMLDLKRRRPDVRAYVAALEQWIIATLDAFNVRGERRENRVGVWVARPDKGAD
HEDKIAAIGVRLKRWVSLHGIAINVEPDLSHFTAIVPCGISDPRYGVTSLVDLGLPVTMADADIALRAAFTEIFGATVDA
PQVDPAVDAGARSPA
>Mature_255_residues
MINAPLHARQTLDLAGFRPQADARPVEWLISEAPVPYPDAVAAMESRAAAIAAGDAAELVWLLEHPPLYTSGTSGQSTDL
LDPRFPLHTTGRGGQLTYHGPGQRVAYVMLDLKRRRPDVRAYVAALEQWIIATLDAFNVRGERRENRVGVWVARPDKGAD
HEDKIAAIGVRLKRWVSLHGIAINVEPDLSHFTAIVPCGISDPRYGVTSLVDLGLPVTMADADIALRAAFTEIFGATVDA
PQVDPAVDAGARSPA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=187, Percent_Identity=35.2941176470588, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI87081767, Length=141, Percent_Identity=43.9716312056738, Blast_Score=124, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6323268, Length=215, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355483, Length=198, Percent_Identity=36.8686868686869, Blast_Score=107, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_RHOP2 (Q2IXI4)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485987.1
- ProteinModelPortal:   Q2IXI4
- SMR:   Q2IXI4
- STRING:   Q2IXI4
- GeneID:   3909370
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2371
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- ProtClustDB:   PRK14341
- BioCyc:   RPAL316058:RPB_2371-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 27506; Mature: 27506

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINAPLHARQTLDLAGFRPQADARPVEWLISEAPVPYPDAVAAMESRAAAIAAGDAAELV
CCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCHHHEE
WLLEHPPLYTSGTSGQSTDLLDPRFPLHTTGRGGQLTYHGPGQRVAYVMLDLKRRRPDVR
EEECCCCCEECCCCCCCCCCCCCCCCCEECCCCCEEEECCCCCEEEEEEEEHHHCCCHHH
AYVAALEQWIIATLDAFNVRGERRENRVGVWVARPDKGADHEDKIAAIGVRLKRWVSLHG
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEE
IAINVEPDLSHFTAIVPCGISDPRYGVTSLVDLGLPVTMADADIALRAAFTEIFGATVDA
EEEEECCCCCCEEEEEECCCCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCC
PQVDPAVDAGARSPA
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MINAPLHARQTLDLAGFRPQADARPVEWLISEAPVPYPDAVAAMESRAAAIAAGDAAELV
CCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCHHHEE
WLLEHPPLYTSGTSGQSTDLLDPRFPLHTTGRGGQLTYHGPGQRVAYVMLDLKRRRPDVR
EEECCCCCEECCCCCCCCCCCCCCCCCEECCCCCEEEECCCCCEEEEEEEEHHHCCCHHH
AYVAALEQWIIATLDAFNVRGERRENRVGVWVARPDKGADHEDKIAAIGVRLKRWVSLHG
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHEEEEEE
IAINVEPDLSHFTAIVPCGISDPRYGVTSLVDLGLPVTMADADIALRAAFTEIFGATVDA
EEEEECCCCCCEEEEEECCCCCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCCC
PQVDPAVDAGARSPA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA