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The map label for this gene is nasA [H]

Identifier: 86748875

GI number: 86748875

Start: 2002749

End: 2005442

Strand: Reverse

Name: nasA [H]

Synonym: RPB_1752

Alternate gene names: 86748875

Gene position: 2005442-2002749 (Counterclockwise)

Preceding gene: 86748876

Following gene: 86748868

Centisome position: 37.61

GC content: 69.52

Gene sequence:

>2694_bases
ATGAACGCACCGACGCAACACCCGCCCGCGGTGCGCACCACCTGCGCCTATTGCGGCGTCGGCTGCGGCGTGCTCGCCAA
GCCCGATGGACGAGGCGGCGCAGCAATCACCGGCGATCCGGAGCATCCGGCGAATTTCGGCCGGCTGTGCTCGAAGGGCT
CGGCGCTCGGCGAAACGCTGGCGCTCGACACAAGGCTGCTGCATCCGATGCGACGCGGCGCCGACAGCGCAATGGCACGC
ACGTCCTGGGATGATGCGCTGGACACGGTTGCGAACGGCTTTGCGGATGTCATCGCCAAGCACGGCCCGGACTCGGTCGC
GTTCTATCTGTCGGGCCAATTGCTCACCGAGGATTACTACGTCGCAAACAAGCTGATGAAGGGTTTTGTCGGCTCGGCCA
ATGTCGACACCAATTCGCGACTGTGCATGGCCTCCTCGGTCGCCGGCCATCGCCGCGCTTTCGGCAGCGACACCGTGCCG
GGGACCTACGCCGATCTCGATCAGGCCGACCTGATCGTGCTGGTCGGCTCCAACGCCGCCTGGTGCCATCCGGTGCTGTA
TCAGCGCATCATCGCCAACAAGCGCGCGCGCGGTGCGACAATCGTGGTGATTGATCCGCGCCGCACCGCGAGCGTCGAGG
ACGACGATTTGCACCTGCCGGTCGCGCCCGGCATGGACACCGTGCTGTTCAGCGGCTTGCTCGTACATCTCGCCGATACG
CTGGCGCTCGACGACGCCTATCTCGAGGCCCACACCACCGGCCTCACCGAGGCGCTGGTGCGCGCTCGGGAGATCGCGCC
GGACATCGCCGCGACGGCGGCTATGACGGGCGTCGACGCGGCCGACATCGCGCGCTTCTTCGCGCTGTTCCGCGCCACAG
AGCGCGTCGTGACCTGTTATTCGCAAGGCGTCAATCAGTCGGCGCAAGGCACCGACAAGGTCAACGCCATCATCAACTGC
CATCTCTCCACCGGGCGGATCGGCCGGCCCGGCATGGGGCCGTTCTCGCTCACCGGGCAGCCCAACGCGATGGGCGGTCG
CGAGGTCGGCGGCCTCGCCAATCAGCTCGCCGCGCATATGGGCTTCGACGCCGAGGCGATCGATCGCGTCGGCCGGTTCT
GGCGCGCGCCGAACATGGCGCGGCGCGAAGGGCTGAAGGCGGTGCAATTGTTCGAGGCGATCGACCGCGGCGAGATCAAG
GCGCTGTGGATCATGGCCACCAACCCGGCAGTGTCGCTGCCCCGCGCCGACGTCGTGCAGGAGGCGCTGCGGAAGCTAGA
CCTCTTGGTGATCTCCGAGAACGTGATCAGCAACGACACCGTCGCCGCCGGCGCGCATGTGCTGCTGCCGGCGGCCGCTT
GGGGCGAGAAGGACGGCACCGTCACCAATTCGGAACGCCGGATCTCGCGGCAACGGGCGTTCCTGCCGCTGCCGGGCGAG
GCGAAGCCGGATTGGTGGAGCGTCGCGCAGGTCGCGCAGCGCATGGGATTCGGCGACGCTTTCGCTTATCACTCGGCCGC
GCAGGTGTTTCGCGAGCACGCCGCACTCTCGGCTTTCGAGAACGACGGCCGCCGCGATTTCGACATCGGCGCGTTGGCCG
ACCTCAGTGACGAGGCCTTCAACGCGCTCGATCCGGTGCAATGGCCGGCGCGCGCGGGCAAGCCTGCGACCGACACGCGA
TTCTTCGCCGACGGGCATTTCTTCACGCCCGATGGTCGCGCGCGGCTGATCGCGCCGGCGATGCCAGCCTTGCACGAAGC
GCCCTCGCCCGCGTTCCCGCTGCTGCTCAACACCGGCCGCGTTCGCGACCAGTGGCACACCATGACTCGCAGCGGCCTCA
GCCCGCGGCTCGGCGCGCATCTGCCGGAGCCGTTCGTCGAGCTGCATCCGGACGATGCGGCAGCGGCCGGCTTGAAGGAC
GGCGGCTTCGCGCGCATCACGACGACGCACGGCGCTTGCATCCTCAAGGTGATCGTCAGTGCCGGTCAACGCCGCGGCTC
GCTGTTCGCGCCGATCCATTGGAGCGGCGAGACCGCGGCGACGGCGCGCGTCGGCGCGCTGGTGGCGCCGGTCACCGATC
CGTTCTCGGGTCAGCCGGAAGCCAAGGCGACGCCGGCCGCGATCGCGCGAGTCGACTACGCGCTGCGCGGCTTCGTGCTG
TCGCGGCAACCGCTGACGCTGTCCGGGGACGATTGGTGGGCACGCGTCGCGGTGCGCAACGGCGCGGGCCTGCTGATCGC
GAGCGACGAACCCGCCTCCGCGTGGCGCGGTCGCGCCGCGACGCTGTTCGGCGACGGCGTCGAGCTCGCCGAGTATCTCG
ACGCACCGCGCCAGAGTTTTCGCGCGGCGGCGTTCGAGAACGGTCGGCTGATCGGCTGCCTGTTCGTCGGCCCGGCCGAG
GCGACGCCGACATGGGACGCGGTGAAGACGCTGTTCGAGGCTGACGCGCTGCCCGATCGGCAGCGCCGCGCCGTGCTGTC
CGGCCGCTCCACCGACGGCGTCGCCGACACCGGACCGATCGTCTGCGCCTGCTTCGGCGTCGGCCTCACCAGCATCCGCG
ACGCGATCGAGAGCGCCGCCGCGATCAGCGTCGACGACATCGGCAGGCACCTGCGCGCCGGCACCAATTGCGGCTCGTGC
CTGCCGGAGCTGCGCAAGATCGTGGCGGGGGCGAAGGTGGGCGAGACGGTCTGA

Upstream 100 bases:

>100_bases
TCGACATCGTCGCGCAGCACGGCGCGCGCACGCCGGCGGAGGCCACCGCCTTCGTCTCCGAGCTGAAGAAGCAGGGCCGC
TACCAGCAGGACGTGTATTG

Downstream 100 bases:

>100_bases
TGAACTAACAACTTGCGCCGGCCTGCTCCCTCGCCCCGCTCTTCGCGGGGAGAGGGGTGGGGTGAGGGGCGACGCGCACA
CGGAGGCTCGGCTGCGCGGA

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 897; Mature: 897

Protein sequence:

>897_residues
MNAPTQHPPAVRTTCAYCGVGCGVLAKPDGRGGAAITGDPEHPANFGRLCSKGSALGETLALDTRLLHPMRRGADSAMAR
TSWDDALDTVANGFADVIAKHGPDSVAFYLSGQLLTEDYYVANKLMKGFVGSANVDTNSRLCMASSVAGHRRAFGSDTVP
GTYADLDQADLIVLVGSNAAWCHPVLYQRIIANKRARGATIVVIDPRRTASVEDDDLHLPVAPGMDTVLFSGLLVHLADT
LALDDAYLEAHTTGLTEALVRAREIAPDIAATAAMTGVDAADIARFFALFRATERVVTCYSQGVNQSAQGTDKVNAIINC
HLSTGRIGRPGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFDAEAIDRVGRFWRAPNMARREGLKAVQLFEAIDRGEIK
ALWIMATNPAVSLPRADVVQEALRKLDLLVISENVISNDTVAAGAHVLLPAAAWGEKDGTVTNSERRISRQRAFLPLPGE
AKPDWWSVAQVAQRMGFGDAFAYHSAAQVFREHAALSAFENDGRRDFDIGALADLSDEAFNALDPVQWPARAGKPATDTR
FFADGHFFTPDGRARLIAPAMPALHEAPSPAFPLLLNTGRVRDQWHTMTRSGLSPRLGAHLPEPFVELHPDDAAAAGLKD
GGFARITTTHGACILKVIVSAGQRRGSLFAPIHWSGETAATARVGALVAPVTDPFSGQPEAKATPAAIARVDYALRGFVL
SRQPLTLSGDDWWARVAVRNGAGLLIASDEPASAWRGRAATLFGDGVELAEYLDAPRQSFRAAAFENGRLIGCLFVGPAE
ATPTWDAVKTLFEADALPDRQRRAVLSGRSTDGVADTGPIVCACFGVGLTSIRDAIESAAAISVDDIGRHLRAGTNCGSC
LPELRKIVAGAKVGETV

Sequences:

>Translated_897_residues
MNAPTQHPPAVRTTCAYCGVGCGVLAKPDGRGGAAITGDPEHPANFGRLCSKGSALGETLALDTRLLHPMRRGADSAMAR
TSWDDALDTVANGFADVIAKHGPDSVAFYLSGQLLTEDYYVANKLMKGFVGSANVDTNSRLCMASSVAGHRRAFGSDTVP
GTYADLDQADLIVLVGSNAAWCHPVLYQRIIANKRARGATIVVIDPRRTASVEDDDLHLPVAPGMDTVLFSGLLVHLADT
LALDDAYLEAHTTGLTEALVRAREIAPDIAATAAMTGVDAADIARFFALFRATERVVTCYSQGVNQSAQGTDKVNAIINC
HLSTGRIGRPGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFDAEAIDRVGRFWRAPNMARREGLKAVQLFEAIDRGEIK
ALWIMATNPAVSLPRADVVQEALRKLDLLVISENVISNDTVAAGAHVLLPAAAWGEKDGTVTNSERRISRQRAFLPLPGE
AKPDWWSVAQVAQRMGFGDAFAYHSAAQVFREHAALSAFENDGRRDFDIGALADLSDEAFNALDPVQWPARAGKPATDTR
FFADGHFFTPDGRARLIAPAMPALHEAPSPAFPLLLNTGRVRDQWHTMTRSGLSPRLGAHLPEPFVELHPDDAAAAGLKD
GGFARITTTHGACILKVIVSAGQRRGSLFAPIHWSGETAATARVGALVAPVTDPFSGQPEAKATPAAIARVDYALRGFVL
SRQPLTLSGDDWWARVAVRNGAGLLIASDEPASAWRGRAATLFGDGVELAEYLDAPRQSFRAAAFENGRLIGCLFVGPAE
ATPTWDAVKTLFEADALPDRQRRAVLSGRSTDGVADTGPIVCACFGVGLTSIRDAIESAAAISVDDIGRHLRAGTNCGSC
LPELRKIVAGAKVGETV
>Mature_897_residues
MNAPTQHPPAVRTTCAYCGVGCGVLAKPDGRGGAAITGDPEHPANFGRLCSKGSALGETLALDTRLLHPMRRGADSAMAR
TSWDDALDTVANGFADVIAKHGPDSVAFYLSGQLLTEDYYVANKLMKGFVGSANVDTNSRLCMASSVAGHRRAFGSDTVP
GTYADLDQADLIVLVGSNAAWCHPVLYQRIIANKRARGATIVVIDPRRTASVEDDDLHLPVAPGMDTVLFSGLLVHLADT
LALDDAYLEAHTTGLTEALVRAREIAPDIAATAAMTGVDAADIARFFALFRATERVVTCYSQGVNQSAQGTDKVNAIINC
HLSTGRIGRPGMGPFSLTGQPNAMGGREVGGLANQLAAHMGFDAEAIDRVGRFWRAPNMARREGLKAVQLFEAIDRGEIK
ALWIMATNPAVSLPRADVVQEALRKLDLLVISENVISNDTVAAGAHVLLPAAAWGEKDGTVTNSERRISRQRAFLPLPGE
AKPDWWSVAQVAQRMGFGDAFAYHSAAQVFREHAALSAFENDGRRDFDIGALADLSDEAFNALDPVQWPARAGKPATDTR
FFADGHFFTPDGRARLIAPAMPALHEAPSPAFPLLLNTGRVRDQWHTMTRSGLSPRLGAHLPEPFVELHPDDAAAAGLKD
GGFARITTTHGACILKVIVSAGQRRGSLFAPIHWSGETAATARVGALVAPVTDPFSGQPEAKATPAAIARVDYALRGFVL
SRQPLTLSGDDWWARVAVRNGAGLLIASDEPASAWRGRAATLFGDGVELAEYLDAPRQSFRAAAFENGRLIGCLFVGPAE
ATPTWDAVKTLFEADALPDRQRRAVLSGRSTDGVADTGPIVCACFGVGLTSIRDAIESAAAISVDDIGRHLRAGTNCGSC
LPELRKIVAGAKVGETV

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=798, Percent_Identity=28.0701754385965, Blast_Score=286, Evalue=4e-78,
Organism=Escherichia coli, GI3868721, Length=701, Percent_Identity=25.3922967189729, Blast_Score=236, Evalue=7e-63,
Organism=Escherichia coli, GI3868720, Length=605, Percent_Identity=23.801652892562, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI3868719, Length=605, Percent_Identity=23.4710743801653, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1787778, Length=593, Percent_Identity=25.6323777403035, Blast_Score=116, Evalue=7e-27,
Organism=Escherichia coli, GI1787870, Length=503, Percent_Identity=22.6640159045726, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI87081797, Length=679, Percent_Identity=21.6494845360825, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 95093; Mature: 95093

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPTQHPPAVRTTCAYCGVGCGVLAKPDGRGGAAITGDPEHPANFGRLCSKGSALGETL
CCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCEECCCCCCCHHHHHHHHCCCHHHHHH
ALDTRLLHPMRRGADSAMARTSWDDALDTVANGFADVIAKHGPDSVAFYLSGQLLTEDYY
HHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECHHHH
VANKLMKGFVGSANVDTNSRLCMASSVAGHRRAFGSDTVPGTYADLDQADLIVLVGSNAA
HHHHHHHHHCCCCCCCCCCCEEEEHHCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCC
WCHPVLYQRIIANKRARGATIVVIDPRRTASVEDDDLHLPVAPGMDTVLFSGLLVHLADT
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHH
LALDDAYLEAHTTGLTEALVRAREIAPDIAATAAMTGVDAADIARFFALFRATERVVTCY
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SQGVNQSAQGTDKVNAIINCHLSTGRIGRPGMGPFSLTGQPNAMGGREVGGLANQLAAHM
HHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHC
GFDAEAIDRVGRFWRAPNMARREGLKAVQLFEAIDRGEIKALWIMATNPAVSLPRADVVQ
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHH
EALRKLDLLVISENVISNDTVAAGAHVLLPAAAWGEKDGTVTNSERRISRQRAFLPLPGE
HHHHHHHEEEEECCCCCCCCHHCCCEEEEEHHHCCCCCCCCCCHHHHHHHHHCCCCCCCC
AKPDWWSVAQVAQRMGFGDAFAYHSAAQVFREHAALSAFENDGRRDFDIGALADLSDEAF
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHH
NALDPVQWPARAGKPATDTRFFADGHFFTPDGRARLIAPAMPALHEAPSPAFPLLLNTGR
HCCCCCCCCHHCCCCCCCCEEEECCCEECCCCCCEEECCCCHHHHCCCCCCCCEEECCCC
VRDQWHTMTRSGLSPRLGAHLPEPFVELHPDDAAAAGLKDGGFARITTTHGACILKVIVS
CHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCHHHCCCCCCCEEEEEECCCHHHHHHHHH
AGQRRGSLFAPIHWSGETAATARVGALVAPVTDPFSGQPEAKATPAAIARVDYALRGFVL
CCCCCCCEEEEEEECCCCHHHHHHCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHEE
SRQPLTLSGDDWWARVAVRNGAGLLIASDEPASAWRGRAATLFGDGVELAEYLDAPRQSF
CCCCCEECCCCEEEEEEEECCCEEEEECCCCHHHHCCCEEEEECCCHHHHHHHCCHHHHH
RAAAFENGRLIGCLFVGPAEATPTWDAVKTLFEADALPDRQRRAVLSGRSTDGVADTGPI
HHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCH
VCACFGVGLTSIRDAIESAAAISVDDIGRHLRAGTNCGSCLPELRKIVAGAKVGETV
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNAPTQHPPAVRTTCAYCGVGCGVLAKPDGRGGAAITGDPEHPANFGRLCSKGSALGETL
CCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCEECCCCCCCHHHHHHHHCCCHHHHHH
ALDTRLLHPMRRGADSAMARTSWDDALDTVANGFADVIAKHGPDSVAFYLSGQLLTEDYY
HHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECEEECHHHH
VANKLMKGFVGSANVDTNSRLCMASSVAGHRRAFGSDTVPGTYADLDQADLIVLVGSNAA
HHHHHHHHHCCCCCCCCCCCEEEEHHCCHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCC
WCHPVLYQRIIANKRARGATIVVIDPRRTASVEDDDLHLPVAPGMDTVLFSGLLVHLADT
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHH
LALDDAYLEAHTTGLTEALVRAREIAPDIAATAAMTGVDAADIARFFALFRATERVVTCY
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
SQGVNQSAQGTDKVNAIINCHLSTGRIGRPGMGPFSLTGQPNAMGGREVGGLANQLAAHM
HHCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHC
GFDAEAIDRVGRFWRAPNMARREGLKAVQLFEAIDRGEIKALWIMATNPAVSLPRADVVQ
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHH
EALRKLDLLVISENVISNDTVAAGAHVLLPAAAWGEKDGTVTNSERRISRQRAFLPLPGE
HHHHHHHEEEEECCCCCCCCHHCCCEEEEEHHHCCCCCCCCCCHHHHHHHHHCCCCCCCC
AKPDWWSVAQVAQRMGFGDAFAYHSAAQVFREHAALSAFENDGRRDFDIGALADLSDEAF
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHH
NALDPVQWPARAGKPATDTRFFADGHFFTPDGRARLIAPAMPALHEAPSPAFPLLLNTGR
HCCCCCCCCHHCCCCCCCCEEEECCCEECCCCCCEEECCCCHHHHCCCCCCCCEEECCCC
VRDQWHTMTRSGLSPRLGAHLPEPFVELHPDDAAAAGLKDGGFARITTTHGACILKVIVS
CHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCHHHCCCCCCCEEEEEECCCHHHHHHHHH
AGQRRGSLFAPIHWSGETAATARVGALVAPVTDPFSGQPEAKATPAAIARVDYALRGFVL
CCCCCCCEEEEEEECCCCHHHHHHCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHEE
SRQPLTLSGDDWWARVAVRNGAGLLIASDEPASAWRGRAATLFGDGVELAEYLDAPRQSF
CCCCCEECCCCEEEEEEEECCCEEEEECCCCHHHHCCCEEEEECCCHHHHHHHCCHHHHH
RAAAFENGRLIGCLFVGPAEATPTWDAVKTLFEADALPDRQRRAVLSGRSTDGVADTGPI
HHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCH
VCACFGVGLTSIRDAIESAAAISVDDIGRHLRAGTNCGSCLPELRKIVAGAKVGETV
HHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]