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The map label for this gene is cysJ [H]

Identifier: 86748876

GI number: 86748876

Start: 2005442

End: 2007061

Strand: Reverse

Name: cysJ [H]

Synonym: RPB_1753

Alternate gene names: 86748876

Gene position: 2007061-2005442 (Counterclockwise)

Preceding gene: 86748877

Following gene: 86748875

Centisome position: 37.64

GC content: 68.21

Gene sequence:

>1620_bases
ATGAGCCAGAATATGCCGCCGCCGATCCCCATGCTGGTCCCGGAGACCGCGCCGTTCTCCGACGAGCAGCGCGCCTGGCT
GAACGGTTTCTTCGCCGGCCTCGTCTCGCTCGATGACGCGGGCGTCACCGCGCTATCGAGCGAACAGGCCGCCGCATTGC
TGGCCGGCGGCCCGGCGCCCACCGCGGAGGACGACGATGGCGGCGCGCCGTGGCACGACCAGACGCTGCCGATCGGGGAG
CGGATGCAGCTCGCCGACGGCAAGCCGTTACGCTGGAAGCTGATGGCCGCGATGGCGCAGCAGGATTGCGGCCAATGCGG
CTACGATTGCCGCAACTACTCGGCGGCGATCTTCGAAGGGAAAGAGACGCGGCTGAATCTATGCGCCCCTGGCGGCAAGG
ACACCGCCCGCATGGTCAAGACGCTGGCCGAGCAGATCGGCAGCGCACCGAAGGCCGACAACGCGCGATCGCTCGCGACC
GATGCGGCGCCCGCCGTGGCGCTGCCGCCGCGCGGCACCTCGCGCGACAATCCGGCCACGGCCAAAGTGCTGTCGCGCCG
CAAGCTGAACAAGGACGGCTCCGAGAAGGAAACCTGGCACATCGAGTTCGACCTCGAAGACGGCCTCGCCTACGAGGTCG
GCGATTCCTTCGGGCTGTTTCCGGGCAACGATCCCAGGCTGGTCGAGCTGGTACTGAAGGCGCTCGGCGCCCCCGCGACG
TTCCCGATCGGCGACCGCACGCTGCGCGAGGCGCTGATCGACAGCGTGTCGCTGGCGCCCGCGCCCGACATGCTGTTCCA
GCTGATCAGCTACATCACCGGTGGCGACAAGCGGAAGAGAGCCCGCGCGCTCGCCAATGGCGAGGATCCGGACGGCGACG
CCGCGACGCTCGACGTGCTGGCGGCGCTGGAGAAGTTTCCCGGCATCCGCCCCGATCCGGAAGCCTTCGTCGAGGCGCTC
GATCCGCTGCAGCCGCGGCTGTATTCGATCTCGTCGTCGCCGAAGACGACTCCCGGCCGCTTGTCGCTGACGGTGGATTG
CGTGCGCTACACCATCGGCAAGCGGCAACGGCTCGGCGTCTGCTCGACCGGCCTCGCCGAACGCGTGACGCCCGGCGACA
CCGTGCGCGTCTATGTGCAGAAGGCGCACAATTTCGCGCTGCCGGCCGATCCGAACCAGCCGATCATCATGATCGGCCCC
GGCACCGGCGTCGCACCCTTCCGCGCCTTCCTGCACGAGCGGCAGGCGGTGGCCGCGCCCGGCAAGAACTGGTTGTTCTT
CGGCCATCAGCGCTCGGCCTGTGATTTCTTCTACGACGACGAACTCAACGCGATGAAGCGCAGCGGTCTCCTCACGCGAC
TGTCGTTGGCATGGTCGCGCGACAGCGGCGAAAAGATCTACGTGCAGGACCGGATGCGCGAGGTCGGCCGCGATCTGTGG
AGCTGGCTCACCGAAGGCGCGAACATCTATGTCTGCGGCGACGCCAAGCGGATGGCCAAGGACGTCGAGCTAGCGCTGGT
CGACATCGTCGCGCAGCACGGCGCGCGCACGCCGGCGGAGGCCACCGCCTTCGTCTCCGAGCTGAAGAAGCAGGGCCGCT
ACCAGCAGGACGTGTATTGA

Upstream 100 bases:

>100_bases
ATCTCGCGCAACGCGCTTCGCCGGACGAAACCTTCCTCGCCTTCGCGCGCCGCCACGACACCCCGACGCTGCAACGTCTG
TTCGCTCTGGAGACCGGTGC

Downstream 100 bases:

>100_bases
TGAACGCACCGACGCAACACCCGCCCGCGGTGCGCACCACCTGCGCCTATTGCGGCGTCGGCTGCGGCGTGCTCGCCAAG
CCCGATGGACGAGGCGGCGC

Product: sulfite reductase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSQNMPPPIPMLVPETAPFSDEQRAWLNGFFAGLVSLDDAGVTALSSEQAAALLAGGPAPTAEDDDGGAPWHDQTLPIGE
RMQLADGKPLRWKLMAAMAQQDCGQCGYDCRNYSAAIFEGKETRLNLCAPGGKDTARMVKTLAEQIGSAPKADNARSLAT
DAAPAVALPPRGTSRDNPATAKVLSRRKLNKDGSEKETWHIEFDLEDGLAYEVGDSFGLFPGNDPRLVELVLKALGAPAT
FPIGDRTLREALIDSVSLAPAPDMLFQLISYITGGDKRKRARALANGEDPDGDAATLDVLAALEKFPGIRPDPEAFVEAL
DPLQPRLYSISSSPKTTPGRLSLTVDCVRYTIGKRQRLGVCSTGLAERVTPGDTVRVYVQKAHNFALPADPNQPIIMIGP
GTGVAPFRAFLHERQAVAAPGKNWLFFGHQRSACDFFYDDELNAMKRSGLLTRLSLAWSRDSGEKIYVQDRMREVGRDLW
SWLTEGANIYVCGDAKRMAKDVELALVDIVAQHGARTPAEATAFVSELKKQGRYQQDVY

Sequences:

>Translated_539_residues
MSQNMPPPIPMLVPETAPFSDEQRAWLNGFFAGLVSLDDAGVTALSSEQAAALLAGGPAPTAEDDDGGAPWHDQTLPIGE
RMQLADGKPLRWKLMAAMAQQDCGQCGYDCRNYSAAIFEGKETRLNLCAPGGKDTARMVKTLAEQIGSAPKADNARSLAT
DAAPAVALPPRGTSRDNPATAKVLSRRKLNKDGSEKETWHIEFDLEDGLAYEVGDSFGLFPGNDPRLVELVLKALGAPAT
FPIGDRTLREALIDSVSLAPAPDMLFQLISYITGGDKRKRARALANGEDPDGDAATLDVLAALEKFPGIRPDPEAFVEAL
DPLQPRLYSISSSPKTTPGRLSLTVDCVRYTIGKRQRLGVCSTGLAERVTPGDTVRVYVQKAHNFALPADPNQPIIMIGP
GTGVAPFRAFLHERQAVAAPGKNWLFFGHQRSACDFFYDDELNAMKRSGLLTRLSLAWSRDSGEKIYVQDRMREVGRDLW
SWLTEGANIYVCGDAKRMAKDVELALVDIVAQHGARTPAEATAFVSELKKQGRYQQDVY
>Mature_538_residues
SQNMPPPIPMLVPETAPFSDEQRAWLNGFFAGLVSLDDAGVTALSSEQAAALLAGGPAPTAEDDDGGAPWHDQTLPIGER
MQLADGKPLRWKLMAAMAQQDCGQCGYDCRNYSAAIFEGKETRLNLCAPGGKDTARMVKTLAEQIGSAPKADNARSLATD
AAPAVALPPRGTSRDNPATAKVLSRRKLNKDGSEKETWHIEFDLEDGLAYEVGDSFGLFPGNDPRLVELVLKALGAPATF
PIGDRTLREALIDSVSLAPAPDMLFQLISYITGGDKRKRARALANGEDPDGDAATLDVLAALEKFPGIRPDPEAFVEALD
PLQPRLYSISSSPKTTPGRLSLTVDCVRYTIGKRQRLGVCSTGLAERVTPGDTVRVYVQKAHNFALPADPNQPIIMIGPG
TGVAPFRAFLHERQAVAAPGKNWLFFGHQRSACDFFYDDELNAMKRSGLLTRLSLAWSRDSGEKIYVQDRMREVGRDLWS
WLTEGANIYVCGDAKRMAKDVELALVDIVAQHGARTPAEATAFVSELKKQGRYQQDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=400, Percent_Identity=34.25, Blast_Score=212, Evalue=9e-55,
Organism=Homo sapiens, GI24041029, Length=403, Percent_Identity=34.2431761786601, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI10835173, Length=435, Percent_Identity=30.5747126436782, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI7657393, Length=409, Percent_Identity=29.5843520782396, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI40254422, Length=407, Percent_Identity=31.9410319410319, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI169790958, Length=267, Percent_Identity=35.2059925093633, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI169790956, Length=267, Percent_Identity=35.2059925093633, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI221316705, Length=418, Percent_Identity=28.9473684210526, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI221316709, Length=409, Percent_Identity=28.8508557457213, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI221316707, Length=366, Percent_Identity=28.9617486338798, Blast_Score=124, Evalue=3e-28,
Organism=Escherichia coli, GI1789123, Length=415, Percent_Identity=41.9277108433735, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17554134, Length=394, Percent_Identity=33.248730964467, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17566446, Length=376, Percent_Identity=28.4574468085106, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17531441, Length=250, Percent_Identity=32.4, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321143, Length=390, Percent_Identity=29.2307692307692, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321832, Length=514, Percent_Identity=28.0155642023346, Blast_Score=136, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6325305, Length=407, Percent_Identity=24.5700245700246, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24582192, Length=399, Percent_Identity=33.3333333333333, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI17137192, Length=399, Percent_Identity=33.3333333333333, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI78706872, Length=374, Percent_Identity=32.8877005347594, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24583543, Length=374, Percent_Identity=32.8877005347594, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI78706876, Length=374, Percent_Identity=32.8877005347594, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24660907, Length=232, Percent_Identity=30.6034482758621, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24660903, Length=232, Percent_Identity=30.6034482758621, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24647438, Length=383, Percent_Identity=25.8485639686684, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 58456; Mature: 58325

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNMPPPIPMLVPETAPFSDEQRAWLNGFFAGLVSLDDAGVTALSSEQAAALLAGGPAP
CCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCC
TAEDDDGGAPWHDQTLPIGERMQLADGKPLRWKLMAAMAQQDCGQCGYDCRNYSAAIFEG
CCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCEEEECC
KETRLNLCAPGGKDTARMVKTLAEQIGSAPKADNARSLATDAAPAVALPPRGTSRDNPAT
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCHH
AKVLSRRKLNKDGSEKETWHIEFDLEDGLAYEVGDSFGLFPGNDPRLVELVLKALGAPAT
HHHHHHHHCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCC
FPIGDRTLREALIDSVSLAPAPDMLFQLISYITGGDKRKRARALANGEDPDGDAATLDVL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
AALEKFPGIRPDPEAFVEALDPLQPRLYSISSSPKTTPGRLSLTVDCVRYTIGKRQRLGV
HHHHHCCCCCCCHHHHHHHHCCCCCHHEECCCCCCCCCCEEEEEHHHHHHHHCCCCCCCC
CSTGLAERVTPGDTVRVYVQKAHNFALPADPNQPIIMIGPGTGVAPFRAFLHERQAVAAP
CCCCHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
GKNWLFFGHQRSACDFFYDDELNAMKRSGLLTRLSLAWSRDSGEKIYVQDRMREVGRDLW
CCCEEEECCCCCCCCEEECCCHHHHHHCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHH
SWLTEGANIYVCGDAKRMAKDVELALVDIVAQHGARTPAEATAFVSELKKQGRYQQDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQNMPPPIPMLVPETAPFSDEQRAWLNGFFAGLVSLDDAGVTALSSEQAAALLAGGPAP
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCCC
TAEDDDGGAPWHDQTLPIGERMQLADGKPLRWKLMAAMAQQDCGQCGYDCRNYSAAIFEG
CCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCEEEECC
KETRLNLCAPGGKDTARMVKTLAEQIGSAPKADNARSLATDAAPAVALPPRGTSRDNPAT
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCHH
AKVLSRRKLNKDGSEKETWHIEFDLEDGLAYEVGDSFGLFPGNDPRLVELVLKALGAPAT
HHHHHHHHCCCCCCCCCEEEEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCC
FPIGDRTLREALIDSVSLAPAPDMLFQLISYITGGDKRKRARALANGEDPDGDAATLDVL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
AALEKFPGIRPDPEAFVEALDPLQPRLYSISSSPKTTPGRLSLTVDCVRYTIGKRQRLGV
HHHHHCCCCCCCHHHHHHHHCCCCCHHEECCCCCCCCCCEEEEEHHHHHHHHCCCCCCCC
CSTGLAERVTPGDTVRVYVQKAHNFALPADPNQPIIMIGPGTGVAPFRAFLHERQAVAAP
CCCCHHHCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
GKNWLFFGHQRSACDFFYDDELNAMKRSGLLTRLSLAWSRDSGEKIYVQDRMREVGRDLW
CCCEEEECCCCCCCCEEECCCHHHHHHCCCCEEEEEEECCCCCCEEEHHHHHHHHHHHHH
SWLTEGANIYVCGDAKRMAKDVELALVDIVAQHGARTPAEATAFVSELKKQGRYQQDVY
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]