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The map label for this gene is fucA [C]

Identifier: 86748775

GI number: 86748775

Start: 1882051

End: 1882824

Strand: Reverse

Name: fucA [C]

Synonym: RPB_1652

Alternate gene names: 86748775

Gene position: 1882824-1882051 (Counterclockwise)

Preceding gene: 86748781

Following gene: 86748774

Centisome position: 35.31

GC content: 65.5

Gene sequence:

>774_bases
ATGACAGCTCACGTACCGCCGGCGGCCATACTCGATCAGACCCGCCAGGCCCGGATCGATCTCGCGGCTGCCTACCGGCT
GATCCACCGGCTCGGGCTCGACGACAGCATCTACACCCACATCTCGGTCAGGCTGCCCGGCCGGCACGACCGTTTCCTGA
TCAACCCCTACGGGATGCGGTTCGAAGAGGTGACGGCGTCGAACCTGGTGGAAGTCGATATCGACGGCAACATCGTCGAC
GATCCGCTCGGCCTCGGCATCAATCCCGCCGGCTTCACCATTCACAGCGCGGTTCACGCCGCGCGGACGGATGCGATGTG
CGTGCTGCACACCCACACCGTCGCCGGCGTCGCGGTGTCCTGCCAACGCCAGGGGCTGCTGCCTCTCAACCAATGGGCGC
TGCAGTTCACCGATCGCCTCGCCTATCACGACTACGAGGGGATCGCGCTCGATCTCGACGAGCGCGCGCGTCTCGTCGCC
GATCTCGGCGACAAGTTCGTCATGGTTCTGCGCAATCACGGCATGCTGACTTGCGGACGATCCGTCGCCGAGGCTTTCAA
GCTGATGCACAATCTGGAGCGGTCCTGCCGCGCCCAGCTCGCGGTGCAGGCGTCCGGCGCCGAGATCAGCCCGCTGTCCG
CCGCCGTCGCCCGCAAGACCGCGGGCCAATACGCGAATTTCTACGACAGCATCGAACTCAGGGGACAGCCCGACAGCGAA
TGGGCCGCCTTCAAGCGGATGCTGGGCCGCACCGATCCCGACTTCATCAACTAG

Upstream 100 bases:

>100_bases
TGGTGGACAATATTTAAGCGCCGATTGTCGACAGTTTAGCCTTGCGGTTGCGTCTTTGTCGCATCAAAAATTGATCCAGA
TCAAAATTGGAACTGCAACG

Downstream 100 bases:

>100_bases
ACCGCGCCCATGTCCATTCCAACGCGCGGATCAGGACATCGAGAAAGAGAGATGCCATGCTGAAGCGCACTTTGATTGGG
CTTGCCCTGGTCTGCGGTCT

Product: class II aldolase/adducin-like

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MTAHVPPAAILDQTRQARIDLAAAYRLIHRLGLDDSIYTHISVRLPGRHDRFLINPYGMRFEEVTASNLVEVDIDGNIVD
DPLGLGINPAGFTIHSAVHAARTDAMCVLHTHTVAGVAVSCQRQGLLPLNQWALQFTDRLAYHDYEGIALDLDERARLVA
DLGDKFVMVLRNHGMLTCGRSVAEAFKLMHNLERSCRAQLAVQASGAEISPLSAAVARKTAGQYANFYDSIELRGQPDSE
WAAFKRMLGRTDPDFIN

Sequences:

>Translated_257_residues
MTAHVPPAAILDQTRQARIDLAAAYRLIHRLGLDDSIYTHISVRLPGRHDRFLINPYGMRFEEVTASNLVEVDIDGNIVD
DPLGLGINPAGFTIHSAVHAARTDAMCVLHTHTVAGVAVSCQRQGLLPLNQWALQFTDRLAYHDYEGIALDLDERARLVA
DLGDKFVMVLRNHGMLTCGRSVAEAFKLMHNLERSCRAQLAVQASGAEISPLSAAVARKTAGQYANFYDSIELRGQPDSE
WAAFKRMLGRTDPDFIN
>Mature_256_residues
TAHVPPAAILDQTRQARIDLAAAYRLIHRLGLDDSIYTHISVRLPGRHDRFLINPYGMRFEEVTASNLVEVDIDGNIVDD
PLGLGINPAGFTIHSAVHAARTDAMCVLHTHTVAGVAVSCQRQGLLPLNQWALQFTDRLAYHDYEGIALDLDERARLVAD
LGDKFVMVLRNHGMLTCGRSVAEAFKLMHNLERSCRAQLAVQASGAEISPLSAAVARKTAGQYANFYDSIELRGQPDSEW
AAFKRMLGRTDPDFIN

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826319, Length=190, Percent_Identity=47.3684210526316, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI29826321, Length=190, Percent_Identity=47.3684210526316, Blast_Score=174, Evalue=5e-44,
Organism=Homo sapiens, GI29826323, Length=190, Percent_Identity=47.3684210526316, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI29826325, Length=192, Percent_Identity=46.875, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI9943848, Length=186, Percent_Identity=40.8602150537634, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI9951927, Length=186, Percent_Identity=40.8602150537634, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI62912453, Length=186, Percent_Identity=40.8602150537634, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI89276787, Length=200, Percent_Identity=36, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI9257190, Length=200, Percent_Identity=36, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI297307160, Length=200, Percent_Identity=36, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI297307158, Length=200, Percent_Identity=36, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI9257192, Length=200, Percent_Identity=36, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI115533218, Length=244, Percent_Identity=38.1147540983607, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI115533216, Length=244, Percent_Identity=38.1147540983607, Blast_Score=165, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71989560, Length=242, Percent_Identity=33.0578512396694, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71989550, Length=242, Percent_Identity=33.0578512396694, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71989541, Length=242, Percent_Identity=33.0578512396694, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71989544, Length=242, Percent_Identity=33.0578512396694, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71989555, Length=242, Percent_Identity=33.0578512396694, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI281363755, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281363751, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24655690, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24655685, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281363763, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281363757, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17136738, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281363761, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281363759, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI281363753, Length=191, Percent_Identity=38.2198952879581, Blast_Score=124, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 28374; Mature: 28242

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHVPPAAILDQTRQARIDLAAAYRLIHRLGLDDSIYTHISVRLPGRHDRFLINPYGMR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEECCCCCC
FEEVTASNLVEVDIDGNIVDDPLGLGINPAGFTIHSAVHAARTDAMCVLHTHTVAGVAVS
HHHCCCCCEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHEEEE
CQRQGLLPLNQWALQFTDRLAYHDYEGIALDLDERARLVADLGDKFVMVLRNHGMLTCGR
HHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCHHEEEEECCCCCEEECH
SVAEAFKLMHNLERSCRAQLAVQASGAEISPLSAAVARKTAGQYANFYDSIELRGQPDSE
HHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCHH
WAAFKRMLGRTDPDFIN
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TAHVPPAAILDQTRQARIDLAAAYRLIHRLGLDDSIYTHISVRLPGRHDRFLINPYGMR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEEECCCCCC
FEEVTASNLVEVDIDGNIVDDPLGLGINPAGFTIHSAVHAARTDAMCVLHTHTVAGVAVS
HHHCCCCCEEEEEECCCEECCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEHHHHHEEEE
CQRQGLLPLNQWALQFTDRLAYHDYEGIALDLDERARLVADLGDKFVMVLRNHGMLTCGR
HHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCHHEEEEECCCCCEEECH
SVAEAFKLMHNLERSCRAQLAVQASGAEISPLSAAVARKTAGQYANFYDSIELRGQPDSE
HHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCHH
WAAFKRMLGRTDPDFIN
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]