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The map label for this gene is ddpA [H]

Identifier: 86748774

GI number: 86748774

Start: 1880444

End: 1881994

Strand: Reverse

Name: ddpA [H]

Synonym: RPB_1651

Alternate gene names: 86748774

Gene position: 1881994-1880444 (Counterclockwise)

Preceding gene: 86748775

Following gene: 86748773

Centisome position: 35.3

GC content: 62.28

Gene sequence:

>1551_bases
ATGCTGAAGCGCACTTTGATTGGGCTTGCCCTGGTCTGCGGTCTGAACGCCGTGGCCGTGGCGGAGGAGCCGAAAGTAGG
CGGCGTCGTGAATGCCGTGATTCAGCCCGAGCCTCCGGGCCTGATGCTGGGTCTGGTCCAGAACGGCCCGACCCAGATGA
TCTCCGGCAACATCTATGATGGCCTGCTACGCTATGGTCCGAAACTCGAGCCGCAGCCCGGCCTCGCCGAGAGCTGGACG
GTGAGCGAGGACGGCAAGGTCTACACCTTCAAGCTCAGGAGCGGCGTGACCTGGCACGACGGCAAACCGTTCACGTCCGC
CGACGTGCTGTTCTCGATCGAATTCCTCAAGCAGACCCACGCCCGCGCCCGTGGCAACCTCGCCACGCTCGACAAGGTGG
AGGCGCCCGACGCCTCGACCGTCGTGTTCACGCTGAAGGAGCCGTTCGGGCCGTTCCTCGGCATCTTCGAAGTCGGCTCG
ATGCCGATGATTCCGAAGCACATCTACGAGGGAACGGATTTCAAGGCCAATCCCGCCAACAACACGCCGATCGGCACCGG
CCCCTACATGTTCAAGGAATGGCAGAAGGGCTCGTTCATCCGGCTGGTGAAGAATCCGAACTACTACATCAAGGGCAAGC
CCCACATCGACGAGATCTACTGGCACGTCATTCCCGACGCGGCGGCGCGGTCGGTGGCGTTCGAGACCGGCAAGATCGAC
GTGCTGCCCGGCGGCTCGGTGGAAAACTTCGACGTACCGCGCCTGAGCAAACTGAAGAACGTCTGCGTCACCGGCGCCGG
CTGGGAATTCTTCAGCCCGCATTCCTGGCTGTGGCTCAACAACCGCTCCGGGCCGACGGCGAACACGAAATTCCGTCAGG
CGCTGATGTTCGCCATCGACCGCAATTTTGCGCGGGACGTGATCTGGAACGGCCTCGGCAAGGTCGCGACGGGCCCCTCC
GGCTCCGCGATCAAATACTATACCAGCGCCGTGCCGAAATACGATCTCGCCCCCGCGAAGGCCAAGGCCCTGCTCAAGGA
AGCCGGCTACAAAGGCGAGAAAGTCCGGATGCTGCCGCTGCCCTACGGCGAAACCTGGCAGCGCTGGGCCGAAGCGGTGA
AGCAGAATCTGCAGGACGTCGGCATCAATGTCGAAATGATCGCCACCGACGTCCCCGGTTGGAACCAGAAAGTCTCCGAC
TGGGACTACGACATCGCCTTCACCTATCTGTATCAGTACGGCGACCCCGCGCTCGGCGTCGGCCGCAATTACGTCAGCAG
CCAGATCGCCAAGGGCTCGCCGTTCAACAACGTCGAAGGTTACTCGAATCCGGAGGTCGACAAGCTGTTCGCCGAGGGCG
CGGTCGCCTTCCCGGATACCAAGCGCGACGAGATCTATGCCAAAGCGCAAAAGATCCTGGTCGAAGACGTGCCGGTCGCA
TGGCTGCTCGAGCTGCAATTTCCGACCATCACCCGCTGCAATGTCAAGAACCTCGTCACCACCGCGATCGGCGTCAACGA
CGGTTTCCGCGACGCCTGGCTCGACAAGTAA

Upstream 100 bases:

>100_bases
TCAAGCGGATGCTGGGCCGCACCGATCCCGACTTCATCAACTAGACCGCGCCCATGTCCATTCCAACGCGCGGATCAGGA
CATCGAGAAAGAGAGATGCC

Downstream 100 bases:

>100_bases
CTTCCCCGACAAGCCGGCGGCGGCGCTCCGTGCCGCCGCCGGTTGACTTCCGAAGCCTAATCTCGAACGCAATGGAAACC
GACGATGCTTGCATTCATTG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MLKRTLIGLALVCGLNAVAVAEEPKVGGVVNAVIQPEPPGLMLGLVQNGPTQMISGNIYDGLLRYGPKLEPQPGLAESWT
VSEDGKVYTFKLRSGVTWHDGKPFTSADVLFSIEFLKQTHARARGNLATLDKVEAPDASTVVFTLKEPFGPFLGIFEVGS
MPMIPKHIYEGTDFKANPANNTPIGTGPYMFKEWQKGSFIRLVKNPNYYIKGKPHIDEIYWHVIPDAAARSVAFETGKID
VLPGGSVENFDVPRLSKLKNVCVTGAGWEFFSPHSWLWLNNRSGPTANTKFRQALMFAIDRNFARDVIWNGLGKVATGPS
GSAIKYYTSAVPKYDLAPAKAKALLKEAGYKGEKVRMLPLPYGETWQRWAEAVKQNLQDVGINVEMIATDVPGWNQKVSD
WDYDIAFTYLYQYGDPALGVGRNYVSSQIAKGSPFNNVEGYSNPEVDKLFAEGAVAFPDTKRDEIYAKAQKILVEDVPVA
WLLELQFPTITRCNVKNLVTTAIGVNDGFRDAWLDK

Sequences:

>Translated_516_residues
MLKRTLIGLALVCGLNAVAVAEEPKVGGVVNAVIQPEPPGLMLGLVQNGPTQMISGNIYDGLLRYGPKLEPQPGLAESWT
VSEDGKVYTFKLRSGVTWHDGKPFTSADVLFSIEFLKQTHARARGNLATLDKVEAPDASTVVFTLKEPFGPFLGIFEVGS
MPMIPKHIYEGTDFKANPANNTPIGTGPYMFKEWQKGSFIRLVKNPNYYIKGKPHIDEIYWHVIPDAAARSVAFETGKID
VLPGGSVENFDVPRLSKLKNVCVTGAGWEFFSPHSWLWLNNRSGPTANTKFRQALMFAIDRNFARDVIWNGLGKVATGPS
GSAIKYYTSAVPKYDLAPAKAKALLKEAGYKGEKVRMLPLPYGETWQRWAEAVKQNLQDVGINVEMIATDVPGWNQKVSD
WDYDIAFTYLYQYGDPALGVGRNYVSSQIAKGSPFNNVEGYSNPEVDKLFAEGAVAFPDTKRDEIYAKAQKILVEDVPVA
WLLELQFPTITRCNVKNLVTTAIGVNDGFRDAWLDK
>Mature_516_residues
MLKRTLIGLALVCGLNAVAVAEEPKVGGVVNAVIQPEPPGLMLGLVQNGPTQMISGNIYDGLLRYGPKLEPQPGLAESWT
VSEDGKVYTFKLRSGVTWHDGKPFTSADVLFSIEFLKQTHARARGNLATLDKVEAPDASTVVFTLKEPFGPFLGIFEVGS
MPMIPKHIYEGTDFKANPANNTPIGTGPYMFKEWQKGSFIRLVKNPNYYIKGKPHIDEIYWHVIPDAAARSVAFETGKID
VLPGGSVENFDVPRLSKLKNVCVTGAGWEFFSPHSWLWLNNRSGPTANTKFRQALMFAIDRNFARDVIWNGLGKVATGPS
GSAIKYYTSAVPKYDLAPAKAKALLKEAGYKGEKVRMLPLPYGETWQRWAEAVKQNLQDVGINVEMIATDVPGWNQKVSD
WDYDIAFTYLYQYGDPALGVGRNYVSSQIAKGSPFNNVEGYSNPEVDKLFAEGAVAFPDTKRDEIYAKAQKILVEDVPVA
WLLELQFPTITRCNVKNLVTTAIGVNDGFRDAWLDK

Specific function: Part of the ABC transporter complex ddpABCDF, which is probably involved in D,D-dipeptide transport [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=503, Percent_Identity=29.4234592445328, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789966, Length=534, Percent_Identity=27.3408239700375, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1787052, Length=492, Percent_Identity=27.4390243902439, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789887, Length=491, Percent_Identity=27.9022403258656, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787495, Length=530, Percent_Identity=23.7735849056604, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI87081878, Length=487, Percent_Identity=24.435318275154, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787551, Length=559, Percent_Identity=23.0769230769231, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789397, Length=478, Percent_Identity=21.5481171548117, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56993; Mature: 56993

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRTLIGLALVCGLNAVAVAEEPKVGGVVNAVIQPEPPGLMLGLVQNGPTQMISGNIYD
CCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCCEECCCHHH
GLLRYGPKLEPQPGLAESWTVSEDGKVYTFKLRSGVTWHDGKPFTSADVLFSIEFLKQTH
HHHHCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCEECCCCCCCCHHHHEEHHHHHHHH
ARARGNLATLDKVEAPDASTVVFTLKEPFGPFLGIFEVGSMPMIPKHIYEGTDFKANPAN
HHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
NTPIGTGPYMFKEWQKGSFIRLVKNPNYYIKGKPHIDEIYWHVIPDAAARSVAFETGKID
CCCCCCCCHHHHHCCCCCEEEEEECCCEEECCCCCHHHEEEEECCCHHHHHEEEECCEEE
VLPGGSVENFDVPRLSKLKNVCVTGAGWEFFSPHSWLWLNNRSGPTANTKFRQALMFAID
EECCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
RNFARDVIWNGLGKVATGPSGSAIKYYTSAVPKYDLAPAKAKALLKEAGYKGEKVRMLPL
HHHHHHHHHCCCCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
PYGETWQRWAEAVKQNLQDVGINVEMIATDVPGWNQKVSDWDYDIAFTYLYQYGDPALGV
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
GRNYVSSQIAKGSPFNNVEGYSNPEVDKLFAEGAVAFPDTKRDEIYAKAQKILVEDVPVA
CCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCEE
WLLELQFPTITRCNVKNLVTTAIGVNDGFRDAWLDK
EEEEECCCCCCCCCHHHHEEEEECCCCCCCHHCCCC
>Mature Secondary Structure
MLKRTLIGLALVCGLNAVAVAEEPKVGGVVNAVIQPEPPGLMLGLVQNGPTQMISGNIYD
CCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCCCEECCCHHH
GLLRYGPKLEPQPGLAESWTVSEDGKVYTFKLRSGVTWHDGKPFTSADVLFSIEFLKQTH
HHHHCCCCCCCCCCCCCCEEECCCCCEEEEEECCCCEECCCCCCCCHHHHEEHHHHHHHH
ARARGNLATLDKVEAPDASTVVFTLKEPFGPFLGIFEVGSMPMIPKHIYEGTDFKANPAN
HHHCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHCCCCCCCHHHHHCCCCCCCCCCC
NTPIGTGPYMFKEWQKGSFIRLVKNPNYYIKGKPHIDEIYWHVIPDAAARSVAFETGKID
CCCCCCCCHHHHHCCCCCEEEEEECCCEEECCCCCHHHEEEEECCCHHHHHEEEECCEEE
VLPGGSVENFDVPRLSKLKNVCVTGAGWEFFSPHSWLWLNNRSGPTANTKFRQALMFAID
EECCCCCCCCCCCHHHHHHHHEEECCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
RNFARDVIWNGLGKVATGPSGSAIKYYTSAVPKYDLAPAKAKALLKEAGYKGEKVRMLPL
HHHHHHHHHCCCCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEC
PYGETWQRWAEAVKQNLQDVGINVEMIATDVPGWNQKVSDWDYDIAFTYLYQYGDPALGV
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCC
GRNYVSSQIAKGSPFNNVEGYSNPEVDKLFAEGAVAFPDTKRDEIYAKAQKILVEDVPVA
CCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCEECCCCCHHHHHHHHHHHHHHCCCEE
WLLELQFPTITRCNVKNLVTTAIGVNDGFRDAWLDK
EEEEECCCCCCCCCHHHHEEEEECCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 9751644 [H]