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The map label for this gene is ygfZ [C]

Identifier: 86748242

GI number: 86748242

Start: 1285992

End: 1286873

Strand: Reverse

Name: ygfZ [C]

Synonym: RPB_1117

Alternate gene names: 86748242

Gene position: 1286873-1285992 (Counterclockwise)

Preceding gene: 86748244

Following gene: 86748241

Centisome position: 24.14

GC content: 68.03

Gene sequence:

>882_bases
ATGAAGGCAGCCCTGCTCCCCGACCGCGGCGTGATCAAGATCAGCGGAGCCGACGCGCGGCATCTGCTGAACGGGCTGGT
CACCACGGATCTGACGCTGCTCGAACCCGGGCTCGGCCGGTTCGGCGCGCTGCTGACGCCGCAGGGCAAGATCGTCGCCG
ATTTCTTCATCACCGAGATCGCCGCGGAGGACGATGGCGGTTTCCTGCTGGATTGCCCGAAAACGCTGGCGGAACCGCTC
ACCACCAAGCTGAAATTCTATAAACTGCGCGCCAAGGTGCTGATCGAGAACCTGTCCGACCGGCTCGGCGTGCTCGCCGT
GTGGGACGGTGCGCCGGCCGCGACGTCGGCACCGGCCTTTACCGATCCGCGCAACGACCAGCTCGGCTGGCGAATCATCG
TGCCCGAACTGCTGGCGCACAAGACCGCCGAGGCGATCGGCGCGGAGCTGGTGGATGCCGCCGCCTATGAGGCGCATCGC
ATCGCCTGCGGTGCGCCGGCCGGCGGCGTCGACTTCGCCTATGGCGATGCGTTTCCGCACGAAGCCAACATGGACCGGCT
GCACGGCGTCGATTTCGGTAAGGGGTGCTACATCGGCCAGGAAGTGGTGTCGCGAATGCACCATCGCGGCACTGCGCGCA
CCCGGATCGTGCGTGTGCTGATCGACGGCGCGGCGCCTCAGCCGGGTAGCGAGATCACCGCCGGCGACAAGAGCGTCGGC
ACCATGGGGTCGTCGGCCGACGGCTGCGGTCTGGCGCTGCTGCGGATCGACCGCGTGGCGGACGCCCGCGAAGCTTCATT
GCCGCTCGGCGCCGCCGGCATCGCGCTGCGCCTGGTCGATCCGGACGAGTTGAAGCCGGCTGTGCGAAAGACCGTGGCAT
GA

Upstream 100 bases:

>100_bases
AACTCTTGTTGGCCGGAGCCCGGCCGCTTACCTTGAAATGTCTTATCCAGCCGGCCGGTTTCCGCAAGGAAGTTCCTGAC
CGGCGCCACGAGAGAATGCA

Downstream 100 bases:

>100_bases
GCGGCGCACGCATGCACGCCGACGGCCTCGCGCGCTGCCCGTGGCCGGGCGACGATCCGCTCTACGTCGCCTATCACGAC
ACCGAATGGGGCGTGCCGGA

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKAALLPDRGVIKISGADARHLLNGLVTTDLTLLEPGLGRFGALLTPQGKIVADFFITEIAAEDDGGFLLDCPKTLAEPL
TTKLKFYKLRAKVLIENLSDRLGVLAVWDGAPAATSAPAFTDPRNDQLGWRIIVPELLAHKTAEAIGAELVDAAAYEAHR
IACGAPAGGVDFAYGDAFPHEANMDRLHGVDFGKGCYIGQEVVSRMHHRGTARTRIVRVLIDGAAPQPGSEITAGDKSVG
TMGSSADGCGLALLRIDRVADAREASLPLGAAGIALRLVDPDELKPAVRKTVA

Sequences:

>Translated_293_residues
MKAALLPDRGVIKISGADARHLLNGLVTTDLTLLEPGLGRFGALLTPQGKIVADFFITEIAAEDDGGFLLDCPKTLAEPL
TTKLKFYKLRAKVLIENLSDRLGVLAVWDGAPAATSAPAFTDPRNDQLGWRIIVPELLAHKTAEAIGAELVDAAAYEAHR
IACGAPAGGVDFAYGDAFPHEANMDRLHGVDFGKGCYIGQEVVSRMHHRGTARTRIVRVLIDGAAPQPGSEITAGDKSVG
TMGSSADGCGLALLRIDRVADAREASLPLGAAGIALRLVDPDELKPAVRKTVA
>Mature_293_residues
MKAALLPDRGVIKISGADARHLLNGLVTTDLTLLEPGLGRFGALLTPQGKIVADFFITEIAAEDDGGFLLDCPKTLAEPL
TTKLKFYKLRAKVLIENLSDRLGVLAVWDGAPAATSAPAFTDPRNDQLGWRIIVPELLAHKTAEAIGAELVDAAAYEAHR
IACGAPAGGVDFAYGDAFPHEANMDRLHGVDFGKGCYIGQEVVSRMHHRGTARTRIVRVLIDGAAPQPGSEITAGDKSVG
TMGSSADGCGLALLRIDRVADAREASLPLGAAGIALRLVDPDELKPAVRKTVA

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Homo sapiens, GI58197556, Length=276, Percent_Identity=30.7971014492754, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71985951, Length=278, Percent_Identity=28.7769784172662, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24645123, Length=278, Percent_Identity=28.4172661870504, Blast_Score=104, Evalue=8e-23,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 30829; Mature: 30829

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAALLPDRGVIKISGADARHLLNGLVTTDLTLLEPGLGRFGALLTPQGKIVADFFITEI
CCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHCCCCCCCCCCEECCCCCEEHHHHHHHH
AAEDDGGFLLDCPKTLAEPLTTKLKFYKLRAKVLIENLSDRLGVLAVWDGAPAATSAPAF
HCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
TDPRNDQLGWRIIVPELLAHKTAEAIGAELVDAAAYEAHRIACGAPAGGVDFAYGDAFPH
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCC
EANMDRLHGVDFGKGCYIGQEVVSRMHHRGTARTRIVRVLIDGAAPQPGSEITAGDKSVG
CCCCHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCC
TMGSSADGCGLALLRIDRVADAREASLPLGAAGIALRLVDPDELKPAVRKTVA
CCCCCCCCCCEEEEEHHHHHCCHHHCCCCCCCCEEEEEECHHHHCHHHHHHCC
>Mature Secondary Structure
MKAALLPDRGVIKISGADARHLLNGLVTTDLTLLEPGLGRFGALLTPQGKIVADFFITEI
CCCCCCCCCCEEEECCCCHHHHHHHHHHCCHHHCCCCCCCCCCEECCCCCEEHHHHHHHH
AAEDDGGFLLDCPKTLAEPLTTKLKFYKLRAKVLIENLSDRLGVLAVWDGAPAATSAPAF
HCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCC
TDPRNDQLGWRIIVPELLAHKTAEAIGAELVDAAAYEAHRIACGAPAGGVDFAYGDAFPH
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCC
EANMDRLHGVDFGKGCYIGQEVVSRMHHRGTARTRIVRVLIDGAAPQPGSEITAGDKSVG
CCCCHHHCCCCCCCCCCHHHHHHHHHHHCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCC
TMGSSADGCGLALLRIDRVADAREASLPLGAAGIALRLVDPDELKPAVRKTVA
CCCCCCCCCCEEEEEHHHHHCCHHHCCCCCCCCEEEEEECHHHHCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA