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The map label for this gene is guaA [H]

Identifier: 86748241

GI number: 86748241

Start: 1285342

End: 1285995

Strand: Reverse

Name: guaA [H]

Synonym: RPB_1116

Alternate gene names: 86748241

Gene position: 1285995-1285342 (Counterclockwise)

Preceding gene: 86748242

Following gene: 86748239

Centisome position: 24.12

GC content: 66.36

Gene sequence:

>654_bases
ATGAGCGGCGCACGCATGCACGCCGACGGCCTCGCGCGCTGCCCGTGGCCGGGCGACGATCCGCTCTACGTCGCCTATCA
CGACACCGAATGGGGCGTGCCGGAATACGACGACCGCGCGCTGTTCGAGAAGCTGATCCTCGACGGCTTCCAGGCCGGGC
TGTCGTGGATCACGATCCTGCGCAAGCGTGACAATTTCCGCCGCGCCTTCGACGATTTCGATCCGGCCAGGATCGCGCGC
TACGACGCGGCGAAGGTGCAGGCGCTGATGAACGACGTCGGCATCGTCCGCAACCGCGCCAAGATCGACGGCGCGATCGG
CAGCGCCAAGGCCTGGCTGACGATCCAGGAGGAAGGCCCGGGCTTTTCGAAACTGCTGTGGGACCATGTCGGCGGCGCGC
CGAAGGTCAACGCGTTCAAGACCACCTTGAGCGTGCCGGCCTCGACGCCGCTGTCGGTGAAAATCTCGAAGGATCTGTCG
GCGCGCGGCTTCAAATTCGTCGGCCCGACCATCGTCTACGCCTTCATGCAGGCGGTGGGCATGGTCAACGACCATCTGGT
GGATTGCCATTGCCACGATCGTTGCGGCGCAGCAAAAAGGCCGGCGCGACGTTCGGCGGCGCCGGCGCGGACGATCGCCC
AGCGTCAGCGCTGA

Upstream 100 bases:

>100_bases
CGGACGCCCGCGAAGCTTCATTGCCGCTCGGCGCCGCCGGCATCGCGCTGCGCCTGGTCGATCCGGACGAGTTGAAGCCG
GCTGTGCGAAAGACCGTGGC

Downstream 100 bases:

>100_bases
TCGGCGACCCGCTTGATCACCAGCTCGGACAGGCTGACGACCAGCTCTTTCAGTGCTTCGTTTTGCTGCTGCAATTCCTG
CAGCGACGATTCGGCGGCGA

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSGARMHADGLARCPWPGDDPLYVAYHDTEWGVPEYDDRALFEKLILDGFQAGLSWITILRKRDNFRRAFDDFDPARIAR
YDAAKVQALMNDVGIVRNRAKIDGAIGSAKAWLTIQEEGPGFSKLLWDHVGGAPKVNAFKTTLSVPASTPLSVKISKDLS
ARGFKFVGPTIVYAFMQAVGMVNDHLVDCHCHDRCGAAKRPARRSAAPARTIAQRQR

Sequences:

>Translated_217_residues
MSGARMHADGLARCPWPGDDPLYVAYHDTEWGVPEYDDRALFEKLILDGFQAGLSWITILRKRDNFRRAFDDFDPARIAR
YDAAKVQALMNDVGIVRNRAKIDGAIGSAKAWLTIQEEGPGFSKLLWDHVGGAPKVNAFKTTLSVPASTPLSVKISKDLS
ARGFKFVGPTIVYAFMQAVGMVNDHLVDCHCHDRCGAAKRPARRSAAPARTIAQRQR
>Mature_216_residues
SGARMHADGLARCPWPGDDPLYVAYHDTEWGVPEYDDRALFEKLILDGFQAGLSWITILRKRDNFRRAFDDFDPARIARY
DAAKVQALMNDVGIVRNRAKIDGAIGSAKAWLTIQEEGPGFSKLLWDHVGGAPKVNAFKTTLSVPASTPLSVKISKDLSA
RGFKFVGPTIVYAFMQAVGMVNDHLVDCHCHDRCGAAKRPARRSAAPARTIAQRQR

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 N-acetyltransferase domains [H]

Homologues:

Organism=Escherichia coli, GI1789971, Length=182, Percent_Identity=50, Blast_Score=206, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR005019
- InterPro:   IPR001962
- InterPro:   IPR001317
- InterPro:   IPR011257
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF03352 Adenine_glyco; PF00733 Asn_synthase; PF00117 GATase; PF00958 GMP_synt_C [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 24012; Mature: 23880

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGARMHADGLARCPWPGDDPLYVAYHDTEWGVPEYDDRALFEKLILDGFQAGLSWITIL
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RKRDNFRRAFDDFDPARIARYDAAKVQALMNDVGIVRNRAKIDGAIGSAKAWLTIQEEGP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
GFSKLLWDHVGGAPKVNAFKTTLSVPASTPLSVKISKDLSARGFKFVGPTIVYAFMQAVG
CHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
MVNDHLVDCHCHDRCGAAKRPARRSAAPARTIAQRQR
HHCCCEEECCCCCCCCCCCCCCHHCCCHHHHHHHCCC
>Mature Secondary Structure 
SGARMHADGLARCPWPGDDPLYVAYHDTEWGVPEYDDRALFEKLILDGFQAGLSWITIL
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RKRDNFRRAFDDFDPARIARYDAAKVQALMNDVGIVRNRAKIDGAIGSAKAWLTIQEEGP
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCC
GFSKLLWDHVGGAPKVNAFKTTLSVPASTPLSVKISKDLSARGFKFVGPTIVYAFMQAVG
CHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHH
MVNDHLVDCHCHDRCGAAKRPARRSAAPARTIAQRQR
HHCCCEEECCCCCCCCCCCCCCHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]