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The map label for this gene is rutF [H]

Identifier: 86748128

GI number: 86748128

Start: 1148691

End: 1149212

Strand: Direct

Name: rutF [H]

Synonym: RPB_1003

Alternate gene names: 86748128

Gene position: 1148691-1149212 (Clockwise)

Preceding gene: 86748127

Following gene: 86748130

Centisome position: 21.54

GC content: 71.46

Gene sequence:

>522_bases
ATGAACATCCGCCCCGCCGCGCTGCATCGCGAATCCGCCGCCGACAGCTTCAAGGCGGCGATGCGCCACCTCGCCGGTGG
CGTCAGCATCGTCACCAGCGGGCAGGACGACGAGCGCAACGGCATCACCGCGACGTCGGTCAGTTCGCTCTCGGCCGAGC
CGCCGGCGCTGATCGCCTGCATCAATCGCAGCTGCGCGCTGCTGCCGGTGATCCGCGAGCGGGGCACGTTCGGCGTCAAC
ATCCTCGCCGCCGGCCATCGCGACCTCGCCGACCGCTTCGCCGGCCGCCACGGCGTGCGCGGAGCGGAGCGCTACGCCGA
CGGCGACTGGATCGAACTGGTGACCGGGGCGCCGCTGCTGAGCGATGCGCTGGCGGCGATCGACTGCACGGTCGACAGCA
TCATCGACTGGAACAGCCACGCGCTGGTGATCGGCCGGGTCGAGGCCGTGCAGCTCGGCGGCGCCTCGCAGGCGCTGGTG
TATTGGCGCGGCGGCTATCACGACATCGGCGGCACCGAGTAG

Upstream 100 bases:

>100_bases
GGCTGCCGCAATTGGCGCTGCGCGCCGGCGTCGCCGACGCCATCGAGGCCACGGCCACCACGCTGTTCCGCAACTGGCTC
GACTCCCTCGCAGGCCGCTC

Downstream 100 bases:

>100_bases
CCGCCCGATCGCATCGACCGCGACCAGCGCCGTCTCACGCTTGCTCGTCTGCCGATATGGAGCGCCGCCCGCTCATTCCG
GGCGCAGCGACACCGGCCCG

Product: flavin reductase-like, FMN-binding

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 173; Mature: 173

Protein sequence:

>173_residues
MNIRPAALHRESAADSFKAAMRHLAGGVSIVTSGQDDERNGITATSVSSLSAEPPALIACINRSCALLPVIRERGTFGVN
ILAAGHRDLADRFAGRHGVRGAERYADGDWIELVTGAPLLSDALAAIDCTVDSIIDWNSHALVIGRVEAVQLGGASQALV
YWRGGYHDIGGTE

Sequences:

>Translated_173_residues
MNIRPAALHRESAADSFKAAMRHLAGGVSIVTSGQDDERNGITATSVSSLSAEPPALIACINRSCALLPVIRERGTFGVN
ILAAGHRDLADRFAGRHGVRGAERYADGDWIELVTGAPLLSDALAAIDCTVDSIIDWNSHALVIGRVEAVQLGGASQALV
YWRGGYHDIGGTE
>Mature_173_residues
MNIRPAALHRESAADSFKAAMRHLAGGVSIVTSGQDDERNGITATSVSSLSAEPPALIACINRSCALLPVIRERGTFGVN
ILAAGHRDLADRFAGRHGVRGAERYADGDWIELVTGAPLLSDALAAIDCTVDSIIDWNSHALVIGRVEAVQLGGASQALV
YWRGGYHDIGGTE

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=153, Percent_Identity=31.3725490196078, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18202; Mature: 18202

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRPAALHRESAADSFKAAMRHLAGGVSIVTSGQDDERNGITATSVSSLSAEPPALIAC
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCEEEE
INRSCALLPVIRERGTFGVNILAAGHRDLADRFAGRHGVRGAERYADGDWIELVTGAPLL
ECCCCEEEHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCHHHCCCCCEEEEEECCCHH
SDALAAIDCTVDSIIDWNSHALVIGRVEAVQLGGASQALVYWRGGYHDIGGTE
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MNIRPAALHRESAADSFKAAMRHLAGGVSIVTSGQDDERNGITATSVSSLSAEPPALIAC
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCEEEE
INRSCALLPVIRERGTFGVNILAAGHRDLADRFAGRHGVRGAERYADGDWIELVTGAPLL
ECCCCEEEHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCHHHCCCCCEEEEEECCCHH
SDALAAIDCTVDSIIDWNSHALVIGRVEAVQLGGASQALVYWRGGYHDIGGTE
HHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA