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The map label for this gene is ilvB [H]

Identifier: 86748123

GI number: 86748123

Start: 1141411

End: 1143090

Strand: Direct

Name: ilvB [H]

Synonym: RPB_0998

Alternate gene names: 86748123

Gene position: 1141411-1143090 (Clockwise)

Preceding gene: 86748122

Following gene: 86748126

Centisome position: 21.41

GC content: 68.69

Gene sequence:

>1680_bases
ATGCATCACATGCTCGAGAGCCGCACCGCCGCCGAGGCGCTGGTCGATCAACTGGCGATCAACGGCGTCAAGCACGTGTT
CTGCGTGCCGGGAGAGAGCTTCCTGCCGGTGCTGGACGCGCTGCGCGATCGCGACATCACCATCACCGTCTGCCGCCATG
AGGGCGGCGCGGCGATGATGGCGGAGGCGATCGGCAAGGCGACCGGCCAGCCCGGCGTCTGTTTCGTCACCCGCGGGCCG
GGGGCGACCAATGCGTCGGCGGGCATTCACGTCGCGCGGCAGGACTCGACGCCGATGATCCTGTTCGTCGGCCAGGTCGA
ACGCGCGGTCAAGGAGCGCGAGGCGTTTCAGGAGCTCGACTATCGCGCCGTGTTCGGCAGCATGACGAAATGGACCACCG
AGATCGACGATGCCGATCGCGTCACCGAGCTGGTGTCGCGCGCGTTCTACACCGCGACCAGCGGCCGGCCGGGGCCGGTG
GTGATCGCGCTGCCGAAGGACGTGCTGAGCGAACGCGTCACCGTCGGCCATGCCCCGGCGTTCAGACCGGTCGAAACCTC
GCCCGGCGACGAGGAGATGGACGAACTCGCGGCGCTGCTCGCCGGCGCCGAGCGACCGCTGATCGTGCTCGGCGGCAGCC
GCTGGAGCCTGAAGGCGCGCGAGCAGATCGAACAGATCGCGACTCGCACCGGGCTGCCGGTCGCGACCAGCTATCGCCGC
GGCACGCTGTTCGACGTGATGCATCCGCGCTATGCGGGTGATCTCGGCCTCGGACCCAATCCGAAGCTGGTGGCGCGCGC
CAAGGCAGCCGATCTCGTGGTGCTGATCGGCGGACGGCTGGGCGAAATTCCGTCGCAGGGCTACACCCTGCTCGACAGTC
CCGCGCCGCAGACCAAGCTGGTGCACATCCATCCCGGCGCCGAGGAACTCGGCCGGGTGTATCGTCCGCAACTCGCGATC
CACGCCTCGCCGGCGCGCTTCGTCGCGGCGCTGGCGAAACGCGACATCGTCGCGCGACCGGAGTGGCGGGACGCCGCCGA
CGCGGCGCATGCCGACTATCTCGCCTGGACCGAGACCGCGACGCCGCAGCCCGGCGACGTCAATCTCGGCGAGGTGATGA
TTTGGCTGCGCGACAACGTGCCGGCCGACACCATCCTGTGCAACGGCGCCGGCAACTACGCGTCGTGGATTCACCGGTTC
TATCGTTTCCGTCACTACATGACCCACATCGCGCCGACCTCGGCCTCGATGGGCTACGGCATGCCGGCCGCGATCGCGAT
GCAGCGGCTGCATCCGGAGCGGTTGGTGCTGTCGGTCAATGGCGACGGCGATTTCCTGATGAGCGGTCAGGAATTCGCCA
TCGCCGTGCAGTATCGGCTGCCGATCGTCGTGGTGGTCTGCGACAACGGCATGTACGGCACCATCCGGATGCATCAGGAG
CGCGAGTTTCCGGGACGCGTCGCCGCCACCGAGCTGCACAATCCGGATTTCGCCGCCTATGCGCGCGCCTTCGGCGGCTT
CGGCGCCAATGTCGAGAAGACGGCCGACTTCCCGGCGGCGTTCACCGCGGCGCGCGCCTCCGGCCTGCCGTCGATCATCC
ATCTGAAGATCGACCCCGACGCGATCCTCCCCGGCGCGACGCTGTCCGGCATCCGCGCGGCGGCGCTGGAAAAGGCGTAG

Upstream 100 bases:

>100_bases
CGAACCCGCCGCGGCTCGCCGACCTCACCGAAACCGAACTCGTGCTCTACGGGCCGTGACGGACACCCGGCCAACGCGAA
AGATCATTCAGGGAAACGCC

Downstream 100 bases:

>100_bases
GACCGATCGGCTTTGATGGGCTGCTCGCCGCCGGCCGCAGCGTCATCCTGAGGTGCACGACGTGCCGCGCCCTGCGCGGC
GCGGCGGGCCTCGAAGGACG

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MHHMLESRTAAEALVDQLAINGVKHVFCVPGESFLPVLDALRDRDITITVCRHEGGAAMMAEAIGKATGQPGVCFVTRGP
GATNASAGIHVARQDSTPMILFVGQVERAVKEREAFQELDYRAVFGSMTKWTTEIDDADRVTELVSRAFYTATSGRPGPV
VIALPKDVLSERVTVGHAPAFRPVETSPGDEEMDELAALLAGAERPLIVLGGSRWSLKAREQIEQIATRTGLPVATSYRR
GTLFDVMHPRYAGDLGLGPNPKLVARAKAADLVVLIGGRLGEIPSQGYTLLDSPAPQTKLVHIHPGAEELGRVYRPQLAI
HASPARFVAALAKRDIVARPEWRDAADAAHADYLAWTETATPQPGDVNLGEVMIWLRDNVPADTILCNGAGNYASWIHRF
YRFRHYMTHIAPTSASMGYGMPAAIAMQRLHPERLVLSVNGDGDFLMSGQEFAIAVQYRLPIVVVVCDNGMYGTIRMHQE
REFPGRVAATELHNPDFAAYARAFGGFGANVEKTADFPAAFTAARASGLPSIIHLKIDPDAILPGATLSGIRAAALEKA

Sequences:

>Translated_559_residues
MHHMLESRTAAEALVDQLAINGVKHVFCVPGESFLPVLDALRDRDITITVCRHEGGAAMMAEAIGKATGQPGVCFVTRGP
GATNASAGIHVARQDSTPMILFVGQVERAVKEREAFQELDYRAVFGSMTKWTTEIDDADRVTELVSRAFYTATSGRPGPV
VIALPKDVLSERVTVGHAPAFRPVETSPGDEEMDELAALLAGAERPLIVLGGSRWSLKAREQIEQIATRTGLPVATSYRR
GTLFDVMHPRYAGDLGLGPNPKLVARAKAADLVVLIGGRLGEIPSQGYTLLDSPAPQTKLVHIHPGAEELGRVYRPQLAI
HASPARFVAALAKRDIVARPEWRDAADAAHADYLAWTETATPQPGDVNLGEVMIWLRDNVPADTILCNGAGNYASWIHRF
YRFRHYMTHIAPTSASMGYGMPAAIAMQRLHPERLVLSVNGDGDFLMSGQEFAIAVQYRLPIVVVVCDNGMYGTIRMHQE
REFPGRVAATELHNPDFAAYARAFGGFGANVEKTADFPAAFTAARASGLPSIIHLKIDPDAILPGATLSGIRAAALEKA
>Mature_559_residues
MHHMLESRTAAEALVDQLAINGVKHVFCVPGESFLPVLDALRDRDITITVCRHEGGAAMMAEAIGKATGQPGVCFVTRGP
GATNASAGIHVARQDSTPMILFVGQVERAVKEREAFQELDYRAVFGSMTKWTTEIDDADRVTELVSRAFYTATSGRPGPV
VIALPKDVLSERVTVGHAPAFRPVETSPGDEEMDELAALLAGAERPLIVLGGSRWSLKAREQIEQIATRTGLPVATSYRR
GTLFDVMHPRYAGDLGLGPNPKLVARAKAADLVVLIGGRLGEIPSQGYTLLDSPAPQTKLVHIHPGAEELGRVYRPQLAI
HASPARFVAALAKRDIVARPEWRDAADAAHADYLAWTETATPQPGDVNLGEVMIWLRDNVPADTILCNGAGNYASWIHRF
YRFRHYMTHIAPTSASMGYGMPAAIAMQRLHPERLVLSVNGDGDFLMSGQEFAIAVQYRLPIVVVVCDNGMYGTIRMHQE
REFPGRVAATELHNPDFAAYARAFGGFGANVEKTADFPAAFTAARASGLPSIIHLKIDPDAILPGATLSGIRAAALEKA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=571, Percent_Identity=25.9194395796848, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI21361361, Length=548, Percent_Identity=22.8102189781022, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI1790104, Length=539, Percent_Identity=29.8701298701299, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI87081685, Length=547, Percent_Identity=27.2394881170018, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1786717, Length=545, Percent_Identity=26.4220183486239, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788716, Length=572, Percent_Identity=27.0979020979021, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787096, Length=544, Percent_Identity=24.4485294117647, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=555, Percent_Identity=25.5855855855856, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17531301, Length=555, Percent_Identity=25.7657657657658, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17542570, Length=565, Percent_Identity=23.1858407079646, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=564, Percent_Identity=27.4822695035461, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6320816, Length=574, Percent_Identity=23.5191637630662, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922626, Length=570, Percent_Identity=24.9122807017544, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60344; Mature: 60344

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHHMLESRTAAEALVDQLAINGVKHVFCVPGESFLPVLDALRDRDITITVCRHEGGAAMM
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCHHHH
AEAIGKATGQPGVCFVTRGPGATNASAGIHVARQDSTPMILFVGQVERAVKEREAFQELD
HHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHCC
YRAVFGSMTKWTTEIDDADRVTELVSRAFYTATSGRPGPVVIALPKDVLSERVTVGHAPA
HHHHHCCHHHEECCCCCHHHHHHHHHHHHHEECCCCCCCEEEECCHHHHHCCEEECCCCC
FRPVETSPGDEEMDELAALLAGAERPLIVLGGSRWSLKAREQIEQIATRTGLPVATSYRR
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEECCCC
GTLFDVMHPRYAGDLGLGPNPKLVARAKAADLVVLIGGRLGEIPSQGYTLLDSPAPQTKL
CCEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEECCCCCCCEE
VHIHPGAEELGRVYRPQLAIHASPARFVAALAKRDIVARPEWRDAADAAHADYLAWTETA
EEECCCHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEECCC
TPQPGDVNLGEVMIWLRDNVPADTILCNGAGNYASWIHRFYRFRHYMTHIAPTSASMGYG
CCCCCCCCHHHEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
MPAAIAMQRLHPERLVLSVNGDGDFLMSGQEFAIAVQYRLPIVVVVCDNGMYGTIRMHQE
CHHHHHHHHCCCEEEEEEECCCCCEEECCCEEEEEEEEECCEEEEEECCCCEEEEEEECC
REFPGRVAATELHNPDFAAYARAFGGFGANVEKTADFPAAFTAARASGLPSIIHLKIDPD
CCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCC
AILPGATLSGIRAAALEKA
CCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MHHMLESRTAAEALVDQLAINGVKHVFCVPGESFLPVLDALRDRDITITVCRHEGGAAMM
CCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCHHHH
AEAIGKATGQPGVCFVTRGPGATNASAGIHVARQDSTPMILFVGQVERAVKEREAFQELD
HHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEEECHHHHHHHHHHHHHHCC
YRAVFGSMTKWTTEIDDADRVTELVSRAFYTATSGRPGPVVIALPKDVLSERVTVGHAPA
HHHHHCCHHHEECCCCCHHHHHHHHHHHHHEECCCCCCCEEEECCHHHHHCCEEECCCCC
FRPVETSPGDEEMDELAALLAGAERPLIVLGGSRWSLKAREQIEQIATRTGLPVATSYRR
CCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEECCCC
GTLFDVMHPRYAGDLGLGPNPKLVARAKAADLVVLIGGRLGEIPSQGYTLLDSPAPQTKL
CCEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEECCCCCCCEE
VHIHPGAEELGRVYRPQLAIHASPARFVAALAKRDIVARPEWRDAADAAHADYLAWTETA
EEECCCHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEECCC
TPQPGDVNLGEVMIWLRDNVPADTILCNGAGNYASWIHRFYRFRHYMTHIAPTSASMGYG
CCCCCCCCHHHEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
MPAAIAMQRLHPERLVLSVNGDGDFLMSGQEFAIAVQYRLPIVVVVCDNGMYGTIRMHQE
CHHHHHHHHCCCEEEEEEECCCCCEEECCCEEEEEEEEECCEEEEEECCCCEEEEEEECC
REFPGRVAATELHNPDFAAYARAFGGFGANVEKTADFPAAFTAARASGLPSIIHLKIDPD
CCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCC
AILPGATLSGIRAAALEKA
CCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626 [H]