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The map label for this gene is mdeA [H]

Identifier: 86748126

GI number: 86748126

Start: 1146374

End: 1147678

Strand: Direct

Name: mdeA [H]

Synonym: RPB_1001

Alternate gene names: 86748126

Gene position: 1146374-1147678 (Clockwise)

Preceding gene: 86748123

Following gene: 86748127

Centisome position: 21.5

GC content: 66.36

Gene sequence:

>1305_bases
ATGCGCAACGAGACGATCGCCATTCACGCCGGCTACGATCCCGATCCGACCACCAAGGCGGTCGCGGTCCCGATCTACCA
GACCGCATCCTACGCCTTCGACAGCGCCGACCACGGCGCCGCATTGTTCAATCTCGAGACCGAAGGCTATCGCTATTCGC
GGATCGCCAATCCGACCAGCGCTGTGCTGGAGAAGCGCGTCGCCGAGCTCGAGGGTGGCGTCGGCGCGCTCGCGGTCGCC
AGCGGCCAGGCGGCGCTGCACTACGCCTTCGTCAACGTCGCCGATCACGGCGGCAACATCGTCTCGGTGCCGCAGCTCTA
CGGCACCACGCACACGCTGCTCTCCCACATCCTGCCGCGCCAGGGCATCCACGGCCGCTTCGCCGAGAACGACAGTGCCG
CGGCGATCGAGAAGCTCATCGATGCCGATACCCGCGCGGTGTTCTGCGAGACCATCGGCAATCCCGCCGGCAATGTCTGC
GATATCGAGCGCATCGCCGAGGTGGCGCATCGGCACGGCGTGCCGCTGATCGTCGACAACACGGTGGCGACGCCGATCCT
GATGAAGCCGTTCGACCACGGCGCCGACATCGTGGTGCATTCGCTGACCAAGTTCCTCGGCGGCCACGGCACGACGCTGG
GCGGCGCGATCGTCGACAGCGGCCGGTTCGACTGGGCGGCGCAGCCGCAGCGCTTTCCGGCGTTCAACCAGCCCGATCAT
TCCTATCACGGCATGGTCTATGCCGAGCGGTTCGGCCCGACGGCCTATATCGAGCGCGCCCGCAGCATCTATCAGCGCAC
CATGGGGTCGGTGCTGTCGCCGTTCAATGCGTTCCTGCTGCTGCAGGGCATCGAGACCGTCGCGCTGCGGATGGAACGCC
ACGTCGAGAACGCCCGCAAGGTGGCGGAGTTCCTGCGCGACGATCCGCGCGTCGCCTGGGTGAACTACACCGGCTTCCCG
GACAGTCCGTATTACGAGCTGGTGCAGAAATATCTCGGCGGCCGGGCGTCGTCGCTGTTCACCTTCGGCATCAAGGGCGG
CCTCGAGGCCGGCAAGAATTTCTACGATTCGCTGCGGCTGATCACCCGGCTGGTCAATATCGGCGACGCCAAATCGCTCG
CCTGCCATCCGGCCTCGACCACGCATCGGCAGATGTCCGCCGAGCAGCAGCGCACCGCCGGCGTGCTGCCGGAGACGATC
CGGCTGTCGATCGGCATCGAACACATCGCCGACATCATCGAAGATCTCGATCAGGCGCTGGCGCAGGCCGGTGGCCGGCG
CACGCAACTGATCGCGGCGGAATAA

Upstream 100 bases:

>100_bases
CATTTTCGAAATTTGTTGTCGATTAAAGATCTCCGCTCTAGCTAGGAAGACGCCTGCGGACTGCGCGTCGATCGCAGCCT
CGGCGATGCGGAGGTGACCG

Downstream 100 bases:

>100_bases
AGCGGCGCGCCGGCATGTCCCTGCTGTTCGACAACGCCGATCCGATCGACAGCCCGATGCTGGTCCCGAGCGGCCGGCGT
GACGGGCGCAGACGCCGCGA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MRNETIAIHAGYDPDPTTKAVAVPIYQTASYAFDSADHGAALFNLETEGYRYSRIANPTSAVLEKRVAELEGGVGALAVA
SGQAALHYAFVNVADHGGNIVSVPQLYGTTHTLLSHILPRQGIHGRFAENDSAAAIEKLIDADTRAVFCETIGNPAGNVC
DIERIAEVAHRHGVPLIVDNTVATPILMKPFDHGADIVVHSLTKFLGGHGTTLGGAIVDSGRFDWAAQPQRFPAFNQPDH
SYHGMVYAERFGPTAYIERARSIYQRTMGSVLSPFNAFLLLQGIETVALRMERHVENARKVAEFLRDDPRVAWVNYTGFP
DSPYYELVQKYLGGRASSLFTFGIKGGLEAGKNFYDSLRLITRLVNIGDAKSLACHPASTTHRQMSAEQQRTAGVLPETI
RLSIGIEHIADIIEDLDQALAQAGGRRTQLIAAE

Sequences:

>Translated_434_residues
MRNETIAIHAGYDPDPTTKAVAVPIYQTASYAFDSADHGAALFNLETEGYRYSRIANPTSAVLEKRVAELEGGVGALAVA
SGQAALHYAFVNVADHGGNIVSVPQLYGTTHTLLSHILPRQGIHGRFAENDSAAAIEKLIDADTRAVFCETIGNPAGNVC
DIERIAEVAHRHGVPLIVDNTVATPILMKPFDHGADIVVHSLTKFLGGHGTTLGGAIVDSGRFDWAAQPQRFPAFNQPDH
SYHGMVYAERFGPTAYIERARSIYQRTMGSVLSPFNAFLLLQGIETVALRMERHVENARKVAEFLRDDPRVAWVNYTGFP
DSPYYELVQKYLGGRASSLFTFGIKGGLEAGKNFYDSLRLITRLVNIGDAKSLACHPASTTHRQMSAEQQRTAGVLPETI
RLSIGIEHIADIIEDLDQALAQAGGRRTQLIAAE
>Mature_434_residues
MRNETIAIHAGYDPDPTTKAVAVPIYQTASYAFDSADHGAALFNLETEGYRYSRIANPTSAVLEKRVAELEGGVGALAVA
SGQAALHYAFVNVADHGGNIVSVPQLYGTTHTLLSHILPRQGIHGRFAENDSAAAIEKLIDADTRAVFCETIGNPAGNVC
DIERIAEVAHRHGVPLIVDNTVATPILMKPFDHGADIVVHSLTKFLGGHGTTLGGAIVDSGRFDWAAQPQRFPAFNQPDH
SYHGMVYAERFGPTAYIERARSIYQRTMGSVLSPFNAFLLLQGIETVALRMERHVENARKVAEFLRDDPRVAWVNYTGFP
DSPYYELVQKYLGGRASSLFTFGIKGGLEAGKNFYDSLRLITRLVNIGDAKSLACHPASTTHRQMSAEQQRTAGVLPETI
RLSIGIEHIADIIEDLDQALAQAGGRRTQLIAAE

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=33.729216152019, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=31.8289786223278, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=29.2857142857143, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1790375, Length=422, Percent_Identity=27.7251184834123, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1789383, Length=433, Percent_Identity=27.0207852193995, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=415, Percent_Identity=31.3253012048193, Blast_Score=205, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17538055, Length=424, Percent_Identity=31.3679245283019, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=31.1320754716981, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323333, Length=430, Percent_Identity=46.5116279069767, Blast_Score=350, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6319307, Length=383, Percent_Identity=34.2036553524804, Blast_Score=193, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6321170, Length=340, Percent_Identity=27.6470588235294, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=24.3847874720358, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=431, Percent_Identity=26.9141531322506, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922554, Length=420, Percent_Identity=31.6666666666667, Blast_Score=195, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47161; Mature: 47161

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNETIAIHAGYDPDPTTKAVAVPIYQTASYAFDSADHGAALFNLETEGYRYSRIANPTS
CCCCEEEEECCCCCCCCCCEEEEEEEHHHHHHCCCCCCCEEEEEEECCCEEEHHCCCHHH
AVLEKRVAELEGGVGALAVASGQAALHYAFVNVADHGGNIVSVPQLYGTTHTLLSHILPR
HHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEEECCCCCEEECHHHHCCHHHHHHHHHCC
QGIHGRFAENDSAAAIEKLIDADTRAVFCETIGNPAGNVCDIERIAEVAHRHGVPLIVDN
CCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHCCCCCCCEECHHHHHHHHHHCCCCEEECC
TVATPILMKPFDHGADIVVHSLTKFLGGHGTTLGGAIVDSGRFDWAAQPQRFPAFNQPDH
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
SYHGMVYAERFGPTAYIERARSIYQRTMGSVLSPFNAFLLLQGIETVALRMERHVENARK
CCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEFLRDDPRVAWVNYTGFPDSPYYELVQKYLGGRASSLFTFGIKGGLEAGKNFYDSLRL
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
ITRLVNIGDAKSLACHPASTTHRQMSAEQQRTAGVLPETIRLSIGIEHIADIIEDLDQAL
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHEEEECHHHHHHHHHHHHHHH
AQAGGRRTQLIAAE
HHCCCCEEEEEECC
>Mature Secondary Structure
MRNETIAIHAGYDPDPTTKAVAVPIYQTASYAFDSADHGAALFNLETEGYRYSRIANPTS
CCCCEEEEECCCCCCCCCCEEEEEEEHHHHHHCCCCCCCEEEEEEECCCEEEHHCCCHHH
AVLEKRVAELEGGVGALAVASGQAALHYAFVNVADHGGNIVSVPQLYGTTHTLLSHILPR
HHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEEECCCCCEEECHHHHCCHHHHHHHHHCC
QGIHGRFAENDSAAAIEKLIDADTRAVFCETIGNPAGNVCDIERIAEVAHRHGVPLIVDN
CCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHCCCCCCCEECHHHHHHHHHHCCCCEEECC
TVATPILMKPFDHGADIVVHSLTKFLGGHGTTLGGAIVDSGRFDWAAQPQRFPAFNQPDH
CCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
SYHGMVYAERFGPTAYIERARSIYQRTMGSVLSPFNAFLLLQGIETVALRMERHVENARK
CCCCEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEFLRDDPRVAWVNYTGFPDSPYYELVQKYLGGRASSLFTFGIKGGLEAGKNFYDSLRL
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
ITRLVNIGDAKSLACHPASTTHRQMSAEQQRTAGVLPETIRLSIGIEHIADIIEDLDQAL
HHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHEEEECHHHHHHHHHHHHHHH
AQAGGRRTQLIAAE
HHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]