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The map label for this gene is ligB [H]

Identifier: 86747998

GI number: 86747998

Start: 999724

End: 1000578

Strand: Reverse

Name: ligB [H]

Synonym: RPB_0872

Alternate gene names: 86747998

Gene position: 1000578-999724 (Counterclockwise)

Preceding gene: 86747999

Following gene: 86747997

Centisome position: 18.77

GC content: 64.91

Gene sequence:

>855_bases
ATGGCCCGCATCACCGCCAGCGTCTACACCTCGCACGTGCCGGCGATCGGCGCCGCGATCGACATGAAGAAGACCGGCGA
AGACTATTGGAAGCCGGTGTTCAAGGGCTACGAGTTCTCCAAGGAATGGCTGAAGAACGAGAAGCCCGACGTGATCTTCC
TCGTCTTCAACGATCACGCCACCGCGTTCAGCCTCGACTTCATTCCGACCTTCGCGATCGGCACCGCGGCCGAATACACG
CCGGCCGACGAAGGCTGGGGACCGCGCCCGGTGCCGAAGGTGATCGGCCATCCGCAGCTCGCCGCGCATATCGCCCAGTC
GGTGATCCAGGACGATTTCGATCTCACCATCGTCAACAAGATGGACGTCGACCACGGCCTCACCGTGCCGCTCAGCCTGA
TGTGCGGCGAGCCGCAGGCCTGGCCCTGCCCGGTGATCCCGTTCGCCGTCAACGTCGTGCAATATCCGGTGCCGTCCGGC
CGGCGCTGCTACATGCTCGGCCAGGCGATCGGCCGCGCGATCCGGTCCTACGACGAAGACCTCAACGTGCAGATCTGGGG
CACCGGCGGCATGAGCCACCAATTGCAGGGCCCGCGCGCCGGCCTGATCAACCGCGAATGGGACAACGCCTTCCTCGATC
AGCTCATTGCCGATCCCGACGCGCTGTCGAAGAAGCCGCATATCGACTACGTTCGCGAGGCGGGCTCCGAGGGCATCGAA
CTGGTGATGTGGCTGATCGCGCGCGGCGCGATGAGTGACGCTGCCGGCGGCCCCAAGCCCAAGGTCGCGCATCGTTTCTA
TCACGTCCCGGCCAGCAACACCGCGGTCGGGCATCTGATTCTGGAGAACGCCTGA

Upstream 100 bases:

>100_bases
CGGGCTCGATGACCGGCATGACCGAAGAGGAATATCGCAACATGATGATCAATGGCGGCCGTTCCGCCGAGGGCAACCGC
TACATCGGGGAGAAGAAGTA

Downstream 100 bases:

>100_bases
TATGGCCAAAGCGATCAAGGTCGCGCTCGCGGGTGCGGGCGCGTTCGGCATCAAACATCTCGACGGCATCAAGAACATCG
ACGGCGTCGAGGTGATCTCG

Product: protocatechuate 4,5-dioxygenase subunit beta

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MARITASVYTSHVPAIGAAIDMKKTGEDYWKPVFKGYEFSKEWLKNEKPDVIFLVFNDHATAFSLDFIPTFAIGTAAEYT
PADEGWGPRPVPKVIGHPQLAAHIAQSVIQDDFDLTIVNKMDVDHGLTVPLSLMCGEPQAWPCPVIPFAVNVVQYPVPSG
RRCYMLGQAIGRAIRSYDEDLNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDQLIADPDALSKKPHIDYVREAGSEGIE
LVMWLIARGAMSDAAGGPKPKVAHRFYHVPASNTAVGHLILENA

Sequences:

>Translated_284_residues
MARITASVYTSHVPAIGAAIDMKKTGEDYWKPVFKGYEFSKEWLKNEKPDVIFLVFNDHATAFSLDFIPTFAIGTAAEYT
PADEGWGPRPVPKVIGHPQLAAHIAQSVIQDDFDLTIVNKMDVDHGLTVPLSLMCGEPQAWPCPVIPFAVNVVQYPVPSG
RRCYMLGQAIGRAIRSYDEDLNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDQLIADPDALSKKPHIDYVREAGSEGIE
LVMWLIARGAMSDAAGGPKPKVAHRFYHVPASNTAVGHLILENA
>Mature_283_residues
ARITASVYTSHVPAIGAAIDMKKTGEDYWKPVFKGYEFSKEWLKNEKPDVIFLVFNDHATAFSLDFIPTFAIGTAAEYTP
ADEGWGPRPVPKVIGHPQLAAHIAQSVIQDDFDLTIVNKMDVDHGLTVPLSLMCGEPQAWPCPVIPFAVNVVQYPVPSGR
RCYMLGQAIGRAIRSYDEDLNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDQLIADPDALSKKPHIDYVREAGSEGIEL
VMWLIARGAMSDAAGGPKPKVAHRFYHVPASNTAVGHLILENA

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786544, Length=157, Percent_Identity=33.7579617834395, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 31188; Mature: 31057

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARITASVYTSHVPAIGAAIDMKKTGEDYWKPVFKGYEFSKEWLKNEKPDVIFLVFNDHA
CCEEEHHHHHHCCCCHHHEECCCCCCHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCC
TAFSLDFIPTFAIGTAAEYTPADEGWGPRPVPKVIGHPQLAAHIAQSVIQDDFDLTIVNK
CEEEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
MDVDHGLTVPLSLMCGEPQAWPCPVIPFAVNVVQYPVPSGRRCYMLGQAIGRAIRSYDED
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHCCCC
LNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDQLIADPDALSKKPHIDYVREAGSEGIE
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHCCCCHHH
LVMWLIARGAMSDAAGGPKPKVAHRFYHVPASNTAVGHLILENA
HHHHHHHHCCCCCCCCCCCCCHHHEEEECCCCCCEEEEEEEECC
>Mature Secondary Structure 
ARITASVYTSHVPAIGAAIDMKKTGEDYWKPVFKGYEFSKEWLKNEKPDVIFLVFNDHA
CEEEHHHHHHCCCCHHHEECCCCCCHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCC
TAFSLDFIPTFAIGTAAEYTPADEGWGPRPVPKVIGHPQLAAHIAQSVIQDDFDLTIVNK
CEEEECCCHHHHHCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEEE
MDVDHGLTVPLSLMCGEPQAWPCPVIPFAVNVVQYPVPSGRRCYMLGQAIGRAIRSYDED
CCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHEECCCCCCCEEEHHHHHHHHHHHHCCCC
LNVQIWGTGGMSHQLQGPRAGLINREWDNAFLDQLIADPDALSKKPHIDYVREAGSEGIE
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCCCCHHHHHHCCCCHHH
LVMWLIARGAMSDAAGGPKPKVAHRFYHVPASNTAVGHLILENA
HHHHHHHHCCCCCCCCCCCCCHHHEEEECCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]