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The map label for this gene is ligA [H]

Identifier: 86747999

GI number: 86747999

Start: 1000578

End: 1000967

Strand: Reverse

Name: ligA [H]

Synonym: RPB_0873

Alternate gene names: 86747999

Gene position: 1000967-1000578 (Counterclockwise)

Preceding gene: 86748000

Following gene: 86747998

Centisome position: 18.77

GC content: 60.0

Gene sequence:

>390_bases
ATGTCCCTCGACAAACCCTATACCGACGTGCCCGGCACCACGATCTTCGACGCCGATATGTCGCGGATGGGCTATCACCT
GAACCAGTTCTGCATGTCGCTGATGAAGGCGGAGAACCGCGCCCGCTTCAAGGCGGACGAACGCGCCTATCTCGACGAAT
GGCCGATGACCGAGGAGCAGAAGCAGGCGGTGCTCGATCGCGATCTCAACCGCTGCATTTCGCTCGGCGGCAACATCTAC
TTCCTCGCCAAGATCGGCGCGACCGACGGCAAGAGCTTCCAGCAGATGGCGGGCTCGATGACCGGCATGACCGAAGAGGA
ATATCGCAACATGATGATCAATGGCGGCCGTTCCGCCGAGGGCAACCGCTACATCGGGGAGAAGAAGTAA

Upstream 100 bases:

>100_bases
CTCGCGGAGACGCCCCCTCACCCCGACCCTCACCCCGCACGCGGGGAGAGGGAGCGATCGCTGCCCGTTTCAACTAACAA
GTCTCAGAGGAGAAACCGCC

Downstream 100 bases:

>100_bases
TGGCCCGCATCACCGCCAGCGTCTACACCTCGCACGTGCCGGCGATCGGCGCCGCGATCGACATGAAGAAGACCGGCGAA
GACTATTGGAAGCCGGTGTT

Product: protocatechuate 4,5-dioxygenase subunit alpha

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSLDKPYTDVPGTTIFDADMSRMGYHLNQFCMSLMKAENRARFKADERAYLDEWPMTEEQKQAVLDRDLNRCISLGGNIY
FLAKIGATDGKSFQQMAGSMTGMTEEEYRNMMINGGRSAEGNRYIGEKK

Sequences:

>Translated_129_residues
MSLDKPYTDVPGTTIFDADMSRMGYHLNQFCMSLMKAENRARFKADERAYLDEWPMTEEQKQAVLDRDLNRCISLGGNIY
FLAKIGATDGKSFQQMAGSMTGMTEEEYRNMMINGGRSAEGNRYIGEKK
>Mature_128_residues
SLDKPYTDVPGTTIFDADMSRMGYHLNQFCMSLMKAENRARFKADERAYLDEWPMTEEQKQAVLDRDLNRCISLGGNIYF
LAKIGATDGKSFQQMAGSMTGMTEEEYRNMMINGGRSAEGNRYIGEKK

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014159
- InterPro:   IPR011986 [H]

Pfam domain/function: PF07746 LigA [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 14690; Mature: 14559

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
8.5 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
7.8 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDKPYTDVPGTTIFDADMSRMGYHLNQFCMSLMKAENRARFKADERAYLDEWPMTEEQ
CCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHH
KQAVLDRDLNRCISLGGNIYFLAKIGATDGKSFQQMAGSMTGMTEEEYRNMMINGGRSAE
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
GNRYIGEKK
CCCCCCCCC
>Mature Secondary Structure 
SLDKPYTDVPGTTIFDADMSRMGYHLNQFCMSLMKAENRARFKADERAYLDEWPMTEEQ
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHH
KQAVLDRDLNRCISLGGNIYFLAKIGATDGKSFQQMAGSMTGMTEEEYRNMMINGGRSAE
HHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCC
GNRYIGEKK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]