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The map label for this gene is dut

Identifier: 86747749

GI number: 86747749

Start: 703057

End: 703515

Strand: Direct

Name: dut

Synonym: RPB_0623

Alternate gene names: 86747749

Gene position: 703057-703515 (Clockwise)

Preceding gene: 86747748

Following gene: 86747750

Centisome position: 13.19

GC content: 70.37

Gene sequence:

>459_bases
ATGAGCCCCGCCATCGCCGTCGAGATCAAGCGCCTGCCCCACGCCGAAGGCCTGCCGCTGCCCGCGTATCAGTCCGCGCA
CGCCGCCGGTCTCGATCTGTGCGCGGCGAACTCCGCGGACGCGCCGCTGTCGCTGGCCCCGGGCTGCCACATGCTGGTTC
CAACGGGTCTCTGCATCGCGCTGCCGCCGAACTACGAGGCGCAGGTACGGCCGCGTTCGGGCCTCGCCGCCAAGCACGGC
GTCACCGTGCTCAACGCGCCCGGCACCATCGACGCCGACTATCGCGGCGAGATCGGCGTGCTGCTGATCAATCACGGCAA
GGAGCCCTTCACCATCCGCCGCGGCGAGCGCATCGCGCAGATGATCATCGCCCCCGTGGTCCGCGCCGAACTGATCAGCG
TCGAGACGCTGTCGGAAACCGCGCGTGGTGTAGGAGGCTTCGGCTCGACCGGCCGCTAG

Upstream 100 bases:

>100_bases
CCGACACCGTCGATGTGGAGTCGTGGCCTTTGATGACCAAGGACGACGTCGCAACCGCGCTGGTCGCGCGAATCGCGCGA
GAACTGGAAGTACACCAATC

Downstream 100 bases:

>100_bases
ATTTTGTGTCAAGCACGCGCGGATTCGTCAGCTGAAAATATACTTTCCTCATTTCGACCCGCGCGCCGGCGCCGTTCACG
GTCTGGACTCTTACCCGCGG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MSPAIAVEIKRLPHAEGLPLPAYQSAHAAGLDLCAANSADAPLSLAPGCHMLVPTGLCIALPPNYEAQVRPRSGLAAKHG
VTVLNAPGTIDADYRGEIGVLLINHGKEPFTIRRGERIAQMIIAPVVRAELISVETLSETARGVGGFGSTGR

Sequences:

>Translated_152_residues
MSPAIAVEIKRLPHAEGLPLPAYQSAHAAGLDLCAANSADAPLSLAPGCHMLVPTGLCIALPPNYEAQVRPRSGLAAKHG
VTVLNAPGTIDADYRGEIGVLLINHGKEPFTIRRGERIAQMIIAPVVRAELISVETLSETARGVGGFGSTGR
>Mature_151_residues
SPAIAVEIKRLPHAEGLPLPAYQSAHAAGLDLCAANSADAPLSLAPGCHMLVPTGLCIALPPNYEAQVRPRSGLAAKHGV
TVLNAPGTIDADYRGEIGVLLINHGKEPFTIRRGERIAQMIIAPVVRAELISVETLSETARGVGGFGSTGR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=132, Percent_Identity=46.969696969697, Blast_Score=111, Evalue=3e-25,
Organism=Homo sapiens, GI4503423, Length=132, Percent_Identity=46.969696969697, Blast_Score=110, Evalue=6e-25,
Organism=Homo sapiens, GI70906441, Length=132, Percent_Identity=46.969696969697, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1790071, Length=137, Percent_Identity=50.3649635036496, Blast_Score=124, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71988561, Length=151, Percent_Identity=43.7086092715232, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319729, Length=133, Percent_Identity=39.8496240601504, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24583610, Length=131, Percent_Identity=41.9847328244275, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921126, Length=131, Percent_Identity=41.9847328244275, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_RHOP2 (Q2J2H6)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484245.1
- ProteinModelPortal:   Q2J2H6
- SMR:   Q2J2H6
- STRING:   Q2J2H6
- GeneID:   3908316
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0623
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- BioCyc:   RPAL316058:RPB_0623-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15790; Mature: 15659

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: BINDING 85-85

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAIAVEIKRLPHAEGLPLPAYQSAHAAGLDLCAANSADAPLSLAPGCHMLVPTGLCIA
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCEEEECCCEEEE
LPPNYEAQVRPRSGLAAKHGVTVLNAPGTIDADYRGEIGVLLINHGKEPFTIRRGERIAQ
ECCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHH
MIIAPVVRAELISVETLSETARGVGGFGSTGR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SPAIAVEIKRLPHAEGLPLPAYQSAHAAGLDLCAANSADAPLSLAPGCHMLVPTGLCIA
CCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCCEEEECCCEEEE
LPPNYEAQVRPRSGLAAKHGVTVLNAPGTIDADYRGEIGVLLINHGKEPFTIRRGERIAQ
ECCCCCEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHH
MIIAPVVRAELISVETLSETARGVGGFGSTGR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA