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The map label for this gene is fabI2 [H]

Identifier: 86747737

GI number: 86747737

Start: 689471

End: 690316

Strand: Reverse

Name: fabI2 [H]

Synonym: RPB_0611

Alternate gene names: 86747737

Gene position: 690316-689471 (Counterclockwise)

Preceding gene: 86747738

Following gene: 86747735

Centisome position: 12.95

GC content: 66.08

Gene sequence:

>846_bases
ATGCAATCACTGATGCAGGGCAAGCGCGGCCTGATCATGGGCGTCGCCAACAATCATTCGCTCGCCTGGGGGATCGCCAA
GACGCTGTCGGCGCACGGCGCCGAGCTGGCCTTCACCTATCAGGGCGAGGCGCTCGGCAAGCGGGTCAAGCCGCTGGCGC
AGTCGCTGAACGCCGAGCTGGTGCTGCCCTGCGACGTCGAGGACATCGCCAGCGTCGATTCGGTGTTCGCGACGCTGAAC
GAGAAGTGGGGCGGGCTCGATTTCATCGTCCACGCCATCGGCTTCTCCGACAAGAACGAGCTGAAGGGCCGCTACGCCGA
CACCACGCGCGAAAATTTCTCGCGCACCATGGTGATCTCCTGCTTCTCCTTCACCGAGATCGCCAAGCGCGCCGCCGAGC
TGATGCCGGAGAGCGGCGGCACCATGATCACGCTGACCTATGGCGGCGCCACCCGGGTGATGCCGAACTACAATGTGATG
GGCGTGGCCAAGGCGGCGCTGGAAGCCAGCGTGCGCTACCTCGCGTCCGACTACGGCCCGCGCAACATCCGCATCAATGC
GATCTCGGCCGGTCCGGTGCGGACGCTGGCCGGCGCCGGCATCGCGGATGCGCGGATGATGTTCAACTACCAGCTCCGCC
ACGCGCCGCTGCGCCGCACGGTGTCGATCGAGGACGTCGGCGGTTCGGCGCTGTATCTGTTGTCGGGGCTGTCCAACGGC
GTCACCGGCGAAGTGCACTTCGTCGATTCCGGCTACAACATCATCTCGATGCCGCACCCCGAGGCGCTGAAGACCGTCGA
CGAAGCCGAAACCGCCGCCGAACAACGCGCCGCGAAGGGCGAGTAG

Upstream 100 bases:

>100_bases
CAGCAGCAGTCGCGGGCCGGAGAGCCCCTCACCCCAACCCTCTCCCCGCAAGAGCGGGGCGAGGGAGCAAGCCCAATGCG
CGGCGGAGCCAGAGAACCAC

Downstream 100 bases:

>100_bases
CGCCGGCGCTGCCACGCCCCATCCGTCATCCTGAGGAGCGCGCCCTTGCGCGCCTCGAAGGATGAAGCGACGGGTGCAAG
CGGCCCATCCTTCGAGGCTC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MQSLMQGKRGLIMGVANNHSLAWGIAKTLSAHGAELAFTYQGEALGKRVKPLAQSLNAELVLPCDVEDIASVDSVFATLN
EKWGGLDFIVHAIGFSDKNELKGRYADTTRENFSRTMVISCFSFTEIAKRAAELMPESGGTMITLTYGGATRVMPNYNVM
GVAKAALEASVRYLASDYGPRNIRINAISAGPVRTLAGAGIADARMMFNYQLRHAPLRRTVSIEDVGGSALYLLSGLSNG
VTGEVHFVDSGYNIISMPHPEALKTVDEAETAAEQRAAKGE

Sequences:

>Translated_281_residues
MQSLMQGKRGLIMGVANNHSLAWGIAKTLSAHGAELAFTYQGEALGKRVKPLAQSLNAELVLPCDVEDIASVDSVFATLN
EKWGGLDFIVHAIGFSDKNELKGRYADTTRENFSRTMVISCFSFTEIAKRAAELMPESGGTMITLTYGGATRVMPNYNVM
GVAKAALEASVRYLASDYGPRNIRINAISAGPVRTLAGAGIADARMMFNYQLRHAPLRRTVSIEDVGGSALYLLSGLSNG
VTGEVHFVDSGYNIISMPHPEALKTVDEAETAAEQRAAKGE
>Mature_281_residues
MQSLMQGKRGLIMGVANNHSLAWGIAKTLSAHGAELAFTYQGEALGKRVKPLAQSLNAELVLPCDVEDIASVDSVFATLN
EKWGGLDFIVHAIGFSDKNELKGRYADTTRENFSRTMVISCFSFTEIAKRAAELMPESGGTMITLTYGGATRVMPNYNVM
GVAKAALEASVRYLASDYGPRNIRINAISAGPVRTLAGAGIADARMMFNYQLRHAPLRRTVSIEDVGGSALYLLSGLSNG
VTGEVHFVDSGYNIISMPHPEALKTVDEAETAAEQRAAKGE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=49.4208494208494, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI2367365, Length=246, Percent_Identity=27.2357723577236, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789378, Length=256, Percent_Identity=26.953125, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082100, Length=258, Percent_Identity=25.5813953488372, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787905, Length=249, Percent_Identity=25.7028112449799, Blast_Score=64, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=210, Percent_Identity=25.2380952380952, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 30150; Mature: 30150

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLMQGKRGLIMGVANNHSLAWGIAKTLSAHGAELAFTYQGEALGKRVKPLAQSLNAEL
CCHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCE
VLPCDVEDIASVDSVFATLNEKWGGLDFIVHAIGFSDKNELKGRYADTTRENFSRTMVIS
EEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHCCCCCCCCHHHHCCEEEEEE
CFSFTEIAKRAAELMPESGGTMITLTYGGATRVMPNYNVMGVAKAALEASVRYLASDYGP
HHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCC
RNIRINAISAGPVRTLAGAGIADARMMFNYQLRHAPLRRTVSIEDVGGSALYLLSGLSNG
CEEEEEEECCCCHHHHHCCCCHHHHHHEEEHHHCCCCCEEEEEECCCCCCEEEECCCCCC
VTGEVHFVDSGYNIISMPHPEALKTVDEAETAAEQRAAKGE
CCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQSLMQGKRGLIMGVANNHSLAWGIAKTLSAHGAELAFTYQGEALGKRVKPLAQSLNAEL
CCHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCE
VLPCDVEDIASVDSVFATLNEKWGGLDFIVHAIGFSDKNELKGRYADTTRENFSRTMVIS
EEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHCCCCCCCCHHHHCCEEEEEE
CFSFTEIAKRAAELMPESGGTMITLTYGGATRVMPNYNVMGVAKAALEASVRYLASDYGP
HHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCC
RNIRINAISAGPVRTLAGAGIADARMMFNYQLRHAPLRRTVSIEDVGGSALYLLSGLSNG
CEEEEEEECCCCHHHHHCCCCHHHHHHEEEHHHCCCCCEEEEEECCCCCCEEEECCCCCC
VTGEVHFVDSGYNIISMPHPEALKTVDEAETAAEQRAAKGE
CCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]