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The map label for this gene is yieH [H]

Identifier: 86747715

GI number: 86747715

Start: 661554

End: 662207

Strand: Direct

Name: yieH [H]

Synonym: RPB_0589

Alternate gene names: 86747715

Gene position: 661554-662207 (Clockwise)

Preceding gene: 86747714

Following gene: 86747716

Centisome position: 12.41

GC content: 70.03

Gene sequence:

>654_bases
TTGAGCTGCGACCTCGTCATCTTCGATTGCGACGGCGTGCTGGTCGACAGCGAAGTGATTTCCTGCCGCGTCCATGCCGA
AACGCTGACCCGTCACGGCTATCCGATCACCACCGAACAGGTCGCCGAGCGCTTCCTCGGCCGCTCCGGCCGCGAGGCGC
GGCGTGAGATCGAGGCCGAGCTCGGCCGCGCCTTCGACGACGCGCTCGAGGCGCAACTGGTCGCCGATGTGCTGCGCAGC
TTCGCCGAAAGCCTCGAACCGATCCCCCACATCAACGAGGCGCTCGGCGCGCTCGCGCAGCCGGTCTGCGTCGCCTCCAG
CGGCACGCTGGAGCGCATCGCCTTCGCGCTGACCCGCACCGGCCTGCACGCGCGCTTCGCGCCGCATCTGTTCTCCGCCG
AGCAGGTCGAGAGCGGCAAGCCGGCGCCCGACCTGTTTCTGCATGCGGCGCGGCAGATGGGCGTGGCGCCGGCGCGCTGC
GTGGTGATCGAGGACAGCGTGCCGGGCATCCTCGGCGCGACGGCCGCCGGCATGACGGTGCTGGGCTTCGTCGGCGGCAG
CCATTGCGGGCCCGGCATGGCGGCGCGGCTGACCGCCGCCGGGGCCGCGACGGTATTCGCAGATATGCGGCAACTTCCTG
AATTAATTCGATAA

Upstream 100 bases:

>100_bases
GCCTGTGCCCGGCCATCCACGTCTTTGGCGTGGCCAACTCAGCAAGACGTGGATGCCCGGGACGAGCCCGGGCATGACGC
GTAGAAGCGAAAGTAGTTGA

Downstream 100 bases:

>100_bases
AATCGGAAACCGGCGGGTCGGGGCGGGGCCGCCGGCTGGATTTTGCCGGCTTTGCGCTGTAAGGCAGGGCAGCTTCACCC
TCGAAACCCGGATTCCATGG

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MSCDLVIFDCDGVLVDSEVISCRVHAETLTRHGYPITTEQVAERFLGRSGREARREIEAELGRAFDDALEAQLVADVLRS
FAESLEPIPHINEALGALAQPVCVASSGTLERIAFALTRTGLHARFAPHLFSAEQVESGKPAPDLFLHAARQMGVAPARC
VVIEDSVPGILGATAAGMTVLGFVGGSHCGPGMAARLTAAGAATVFADMRQLPELIR

Sequences:

>Translated_217_residues
MSCDLVIFDCDGVLVDSEVISCRVHAETLTRHGYPITTEQVAERFLGRSGREARREIEAELGRAFDDALEAQLVADVLRS
FAESLEPIPHINEALGALAQPVCVASSGTLERIAFALTRTGLHARFAPHLFSAEQVESGKPAPDLFLHAARQMGVAPARC
VVIEDSVPGILGATAAGMTVLGFVGGSHCGPGMAARLTAAGAATVFADMRQLPELIR
>Mature_216_residues
SCDLVIFDCDGVLVDSEVISCRVHAETLTRHGYPITTEQVAERFLGRSGREARREIEAELGRAFDDALEAQLVADVLRSF
AESLEPIPHINEALGALAQPVCVASSGTLERIAFALTRTGLHARFAPHLFSAEQVESGKPAPDLFLHAARQMGVAPARCV
VIEDSVPGILGATAAGMTVLGFVGGSHCGPGMAARLTAAGAATVFADMRQLPELIR

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=214, Percent_Identity=33.6448598130841, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1789046, Length=174, Percent_Identity=31.6091954022989, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082080, Length=189, Percent_Identity=31.7460317460317, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788021, Length=182, Percent_Identity=30.2197802197802, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 22961; Mature: 22829

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCDLVIFDCDGVLVDSEVISCRVHAETLTRHGYPITTEQVAERFLGRSGREARREIEAE
CCCCEEEECCCCEEECHHHHHEEHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
LGRAFDDALEAQLVADVLRSFAESLEPIPHINEALGALAQPVCVASSGTLERIAFALTRT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHH
GLHARFAPHLFSAEQVESGKPAPDLFLHAARQMGVAPARCVVIEDSVPGILGATAAGMTV
CCHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHH
LGFVGGSHCGPGMAARLTAAGAATVFADMRQLPELIR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SCDLVIFDCDGVLVDSEVISCRVHAETLTRHGYPITTEQVAERFLGRSGREARREIEAE
CCCEEEECCCCEEECHHHHHEEHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHH
LGRAFDDALEAQLVADVLRSFAESLEPIPHINEALGALAQPVCVASSGTLERIAFALTRT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHH
GLHARFAPHLFSAEQVESGKPAPDLFLHAARQMGVAPARCVVIEDSVPGILGATAAGMTV
CCHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHH
LGFVGGSHCGPGMAARLTAAGAATVFADMRQLPELIR
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]