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The map label for this gene is miaB [H]

Identifier: 86747716

GI number: 86747716

Start: 662304

End: 663710

Strand: Direct

Name: miaB [H]

Synonym: RPB_0590

Alternate gene names: 86747716

Gene position: 662304-663710 (Clockwise)

Preceding gene: 86747715

Following gene: 86747717

Centisome position: 12.42

GC content: 68.8

Gene sequence:

>1407_bases
ATGGCCCCGCCGCGCAAGCTGCACATCAAGTCCTATGGCTGCCAGATGAACGTCTACGATGCCCAGCGCATGGTCGACGT
GCTGGCGCCGGAAGGCTTCGTCGAGACCGCGAGCGTGGACGACGCCGATCTGGTGATCCTCAACACCTGCCACATCCGCG
AAAAGGCCTCCGAGAAGGTGTTTTCCGAGCTCGGCCGGCTGCGGCTGGCGCGTGACGAGGCGTCGCGCGATGGCCGGCGG
ATGCAGATCGTCGTCGCCGGCTGCGTCGCCCAGGCCGAGGGCGACGAGATCGTCCGCCGCCAGCCCGCCGTCGATGTCGT
GGTCGGCCCGCAGAGCTATCACCATCTGCCGCGCCTATTGGCGCAGGCGGCGCAGACCGGCCGGGCGCTGGAGACCGAGT
TCCCGATCGCCGACAAATTCGGCTTCCTGCCGCAGCCGCAGCCCGAGGCGATCCGGGCGCGCGGCATCTCGGCTTTCGTC
ACCGTGCAGGAAGGCTGCGACAAGTTCTGCACCTTCTGCGTGGTGCCTTATACCCGCGGCGCCGAGGTCTCGCGCCCGGT
GGCGGCGATCCTCGCCGACGTCGAACGGCTCGCCGACCACGGCGTCCGCGAGCTCACCCTGATCGGCCAGAACGTCAACG
CCTATCACGGCGACGGGCCGGACGGCCGGCCCTGGACGCTCGGCCGCCTGCTGCAGCGGCTGGCCGCGGTTCCCGGCATC
GTCCGGCTGCGCTATTCGACCAGCCATCCCAACGACGTCGACGACGACCTGATCGCGGCGCATCGCGACCTCGACGCGCT
GATGCCGTTCGTGCATCTGCCGGTGCAGTCGGGCTCGGACCGGATTCTGGCGGCGATGAACCGCAAGCATACGGCTGCGG
ATTATCGCCGGGTGATCGACCGGTTCCGCGCGGTGCGGCCGGAGATCGCGTTTTCGTCGGATTTCATCGTCGGATTCCCC
GGCGAGACCGATGCCGATTTCGAAGCGACGCTCGCACTGGTCACACAAATCGGCTACGCTGGTGCGTATTCGTTCAAATA
TTCGCCGCGGCCCGGCACGCCCGCGGCGGAGATGCCGGAGATGGTGCCCGCAGCGGTGATGGACGAACGTTTGGAGCGGC
TTCAGCAAGTGATCGACGCTCAGCAATCGGCCTTCAACAGGGCCGCGATCGGCCGGACCGTCGACGTGCTGTTCGAGCGC
GCCGGGCGCAAGCCGGGCCAGATCGTCGGCCGCACCGCCTATCTGCAGCCCGCCCACGTGTTCCCACCCGCCGGGATGGC
GCCCGACAGTCTCGTCGGGCAGATCCTCCCGGTCCGGGTCGACAGCCTCGAGCGTTACAGCCTGCTCGGCGAACTGGCGG
CCACGCCGCCGCGGCATGCTCGTCCTCTGGCCGCCACAGGAGCCTGA

Upstream 100 bases:

>100_bases
ATAAAATCGGAAACCGGCGGGTCGGGGCGGGGCCGCCGGCTGGATTTTGCCGGCTTTGCGCTGTAAGGCAGGGCAGCTTC
ACCCTCGAAACCCGGATTCC

Downstream 100 bases:

>100_bases
GCCCCTTGGCAAAAAGCGCATCGGAGTCGTCCTCGCTGATGTCCCGCCGCAAGCCCGATCGCGATTCCCTGACCCCGCCC
GAAGCCCAGCCCGAGACTCA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MAPPRKLHIKSYGCQMNVYDAQRMVDVLAPEGFVETASVDDADLVILNTCHIREKASEKVFSELGRLRLARDEASRDGRR
MQIVVAGCVAQAEGDEIVRRQPAVDVVVGPQSYHHLPRLLAQAAQTGRALETEFPIADKFGFLPQPQPEAIRARGISAFV
TVQEGCDKFCTFCVVPYTRGAEVSRPVAAILADVERLADHGVRELTLIGQNVNAYHGDGPDGRPWTLGRLLQRLAAVPGI
VRLRYSTSHPNDVDDDLIAAHRDLDALMPFVHLPVQSGSDRILAAMNRKHTAADYRRVIDRFRAVRPEIAFSSDFIVGFP
GETDADFEATLALVTQIGYAGAYSFKYSPRPGTPAAEMPEMVPAAVMDERLERLQQVIDAQQSAFNRAAIGRTVDVLFER
AGRKPGQIVGRTAYLQPAHVFPPAGMAPDSLVGQILPVRVDSLERYSLLGELAATPPRHARPLAATGA

Sequences:

>Translated_468_residues
MAPPRKLHIKSYGCQMNVYDAQRMVDVLAPEGFVETASVDDADLVILNTCHIREKASEKVFSELGRLRLARDEASRDGRR
MQIVVAGCVAQAEGDEIVRRQPAVDVVVGPQSYHHLPRLLAQAAQTGRALETEFPIADKFGFLPQPQPEAIRARGISAFV
TVQEGCDKFCTFCVVPYTRGAEVSRPVAAILADVERLADHGVRELTLIGQNVNAYHGDGPDGRPWTLGRLLQRLAAVPGI
VRLRYSTSHPNDVDDDLIAAHRDLDALMPFVHLPVQSGSDRILAAMNRKHTAADYRRVIDRFRAVRPEIAFSSDFIVGFP
GETDADFEATLALVTQIGYAGAYSFKYSPRPGTPAAEMPEMVPAAVMDERLERLQQVIDAQQSAFNRAAIGRTVDVLFER
AGRKPGQIVGRTAYLQPAHVFPPAGMAPDSLVGQILPVRVDSLERYSLLGELAATPPRHARPLAATGA
>Mature_467_residues
APPRKLHIKSYGCQMNVYDAQRMVDVLAPEGFVETASVDDADLVILNTCHIREKASEKVFSELGRLRLARDEASRDGRRM
QIVVAGCVAQAEGDEIVRRQPAVDVVVGPQSYHHLPRLLAQAAQTGRALETEFPIADKFGFLPQPQPEAIRARGISAFVT
VQEGCDKFCTFCVVPYTRGAEVSRPVAAILADVERLADHGVRELTLIGQNVNAYHGDGPDGRPWTLGRLLQRLAAVPGIV
RLRYSTSHPNDVDDDLIAAHRDLDALMPFVHLPVQSGSDRILAAMNRKHTAADYRRVIDRFRAVRPEIAFSSDFIVGFPG
ETDADFEATLALVTQIGYAGAYSFKYSPRPGTPAAEMPEMVPAAVMDERLERLQQVIDAQQSAFNRAAIGRTVDVLFERA
GRKPGQIVGRTAYLQPAHVFPPAGMAPDSLVGQILPVRVDSLERYSLLGELAATPPRHARPLAATGA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=446, Percent_Identity=33.4080717488789, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI28872784, Length=446, Percent_Identity=33.4080717488789, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI93277076, Length=390, Percent_Identity=30.7692307692308, Blast_Score=178, Evalue=9e-45,
Organism=Escherichia coli, GI1786882, Length=465, Percent_Identity=44.7311827956989, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1787057, Length=473, Percent_Identity=29.5983086680761, Blast_Score=191, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17553146, Length=420, Percent_Identity=34.0476190476191, Blast_Score=241, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI71996771, Length=385, Percent_Identity=25.4545454545455, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356207, Length=494, Percent_Identity=33.1983805668016, Blast_Score=268, Evalue=6e-72,
Organism=Drosophila melanogaster, GI19922432, Length=460, Percent_Identity=26.7391304347826, Blast_Score=161, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51178; Mature: 51047

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPPRKLHIKSYGCQMNVYDAQRMVDVLAPEGFVETASVDDADLVILNTCHIREKASEKV
CCCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHH
FSELGRLRLARDEASRDGRRMQIVVAGCVAQAEGDEIVRRQPAVDVVVGPQSYHHLPRLL
HHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHHH
AQAAQTGRALETEFPIADKFGFLPQPQPEAIRARGISAFVTVQEGCDKFCTFCVVPYTRG
HHHHHCCCCCCCCCCCHHHCCCCCCCCCHHHHHCCCEEEEEHHHHHHHHHHEEECCCCCC
AEVSRPVAAILADVERLADHGVRELTLIGQNVNAYHGDGPDGRPWTLGRLLQRLAAVPGI
CCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
VRLRYSTSHPNDVDDDLIAAHRDLDALMPFVHLPVQSGSDRILAAMNRKHTAADYRRVID
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHH
RFRAVRPEIAFSSDFIVGFPGETDADFEATLALVTQIGYAGAYSFKYSPRPGTPAAEMPE
HHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MVPAAVMDERLERLQQVIDAQQSAFNRAAIGRTVDVLFERAGRKPGQIVGRTAYLQPAHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEECCCCC
FPPAGMAPDSLVGQILPVRVDSLERYSLLGELAATPPRHARPLAATGA
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
APPRKLHIKSYGCQMNVYDAQRMVDVLAPEGFVETASVDDADLVILNTCHIREKASEKV
CCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCEEECCCCCCCEEEEEEHHHHHHHHHHH
FSELGRLRLARDEASRDGRRMQIVVAGCVAQAEGDEIVRRQPAVDVVVGPQSYHHLPRLL
HHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCHHHHCCCCEEEEECCHHHHHHHHHH
AQAAQTGRALETEFPIADKFGFLPQPQPEAIRARGISAFVTVQEGCDKFCTFCVVPYTRG
HHHHHCCCCCCCCCCCHHHCCCCCCCCCHHHHHCCCEEEEEHHHHHHHHHHEEECCCCCC
AEVSRPVAAILADVERLADHGVRELTLIGQNVNAYHGDGPDGRPWTLGRLLQRLAAVPGI
CCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCE
VRLRYSTSHPNDVDDDLIAAHRDLDALMPFVHLPVQSGSDRILAAMNRKHTAADYRRVID
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHH
RFRAVRPEIAFSSDFIVGFPGETDADFEATLALVTQIGYAGAYSFKYSPRPGTPAAEMPE
HHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MVPAAVMDERLERLQQVIDAQQSAFNRAAIGRTVDVLFERAGRKPGQIVGRTAYLQPAHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEECCCCC
FPPAGMAPDSLVGQILPVRVDSLERYSLLGELAATPPRHARPLAATGA
CCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA