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The map label for this gene is trpS [H]

Identifier: 86747709

GI number: 86747709

Start: 657004

End: 658056

Strand: Direct

Name: trpS [H]

Synonym: RPB_0583

Alternate gene names: 86747709

Gene position: 657004-658056 (Clockwise)

Preceding gene: 86747708

Following gene: 86747710

Centisome position: 12.32

GC content: 64.01

Gene sequence:

>1053_bases
ATGGCCATCACGCAGCGGGTTTTCTCCGGGGTCCAGCCGACCGGCAATCTGCATCTCGGCAACTACCTCGGCGCGATCGT
GAATTTCGTGAAGCTGCAGGAGACTCACAACTGCATCTATTGCGTGGTCGATCTGCACGCCATCACCGTGCCGGTGACGG
TCTGGGGCGGCCCGGACGAATTGCGTCGCAACACCCGCGAGGTCACCGCGGCGTTCATCGCCGCCGGCATCGATCCGCAG
AAGCACATCATCTTCAACCAGAGCCAAGTCGCCGAACACGCCGAACTCGCCTGGGTGTTCAATTGCGTCGCCCGGCTCGG
CTGGCTCAACCGCATGACCCAGTTCAAGGAGAAGGCCGGCAAGGACCGCGAGAACGCCTCGATTGGACTATATGATTATC
CGGTGCTGATGGCGTCCGACATTCTGGTCTACCGCGCCACCCATGTGCCGGTCGGCGAGGACCAGAAGCAGCATCTGGAA
CTGACCCGCGACATCGCCCAGAAGTTCAACAACGACTTCTCCGCCTCGATCGCCGCGCAGGGCCTCGGCGACGCTTACTT
CCCGCTGCCCGAGCCGGTGATCACCGGGCCTGCGACCCGGGTGATGAGCCTGCGCGACGGCACCAAGAAGATGTCGAAAT
CGGATCCGTCGGACTACTCGCGCATCAACCTCACCGACGACGCCGACGCGATCGCGCAGAAGATCCGCAAGGCCAAGACC
GATCCGGAGCCGCTGCCGACCGAGGAGAAGGGGCTGGAGCCGCGCCCGGAGGCCGACAATCTGGTCGGCATCTTTGCGGC
GCTGGCCGGCAAGCCGAAGGCCCAGGTGCTGAGCGAATTCGGCGGCGGGCAGTTCTCCGCATTCAAGTCGAATCTGGTCG
ATCTCGCGGTCGAGAAATTGTCGCCGATCGCCGGCGAGATGAAGCGGCTGTCGGCCGACCACGGCTATGTCGATTCGGTG
CTGGCATCGGGCAGCGACCGCGCCCGCGTGATCGCCGCCGAAACCATGACGGCGGTCAAGGACATCATGGGCATGGTGCG
GAAGAAGGGCTGA

Upstream 100 bases:

>100_bases
CAGCGGCGCTCGGGGCGCTGCGGCGGCCGCGCGGCTTGCGCGGCTAGCGCGCTTGTGCCACTTCACGGCGCCTTTCCCAG
TACGCGCAGGAATACGACCA

Downstream 100 bases:

>100_bases
GCTGTTCTTTCGCCGCCCGCTACGCCCTCTCCGTCATCCTGAGGTGCGCGCCCTTGCGCGCCTCGAAGGATGGCCGCAGG
CACCTCGCGCGCAGCCGCAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MAITQRVFSGVQPTGNLHLGNYLGAIVNFVKLQETHNCIYCVVDLHAITVPVTVWGGPDELRRNTREVTAAFIAAGIDPQ
KHIIFNQSQVAEHAELAWVFNCVARLGWLNRMTQFKEKAGKDRENASIGLYDYPVLMASDILVYRATHVPVGEDQKQHLE
LTRDIAQKFNNDFSASIAAQGLGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADAIAQKIRKAKT
DPEPLPTEEKGLEPRPEADNLVGIFAALAGKPKAQVLSEFGGGQFSAFKSNLVDLAVEKLSPIAGEMKRLSADHGYVDSV
LASGSDRARVIAAETMTAVKDIMGMVRKKG

Sequences:

>Translated_350_residues
MAITQRVFSGVQPTGNLHLGNYLGAIVNFVKLQETHNCIYCVVDLHAITVPVTVWGGPDELRRNTREVTAAFIAAGIDPQ
KHIIFNQSQVAEHAELAWVFNCVARLGWLNRMTQFKEKAGKDRENASIGLYDYPVLMASDILVYRATHVPVGEDQKQHLE
LTRDIAQKFNNDFSASIAAQGLGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADAIAQKIRKAKT
DPEPLPTEEKGLEPRPEADNLVGIFAALAGKPKAQVLSEFGGGQFSAFKSNLVDLAVEKLSPIAGEMKRLSADHGYVDSV
LASGSDRARVIAAETMTAVKDIMGMVRKKG
>Mature_349_residues
AITQRVFSGVQPTGNLHLGNYLGAIVNFVKLQETHNCIYCVVDLHAITVPVTVWGGPDELRRNTREVTAAFIAAGIDPQK
HIIFNQSQVAEHAELAWVFNCVARLGWLNRMTQFKEKAGKDRENASIGLYDYPVLMASDILVYRATHVPVGEDQKQHLEL
TRDIAQKFNNDFSASIAAQGLGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADAIAQKIRKAKTD
PEPLPTEEKGLEPRPEADNLVGIFAALAGKPKAQVLSEFGGGQFSAFKSNLVDLAVEKLSPIAGEMKRLSADHGYVDSVL
ASGSDRARVIAAETMTAVKDIMGMVRKKG

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=345, Percent_Identity=41.7391304347826, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI41352700, Length=192, Percent_Identity=47.9166666666667, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1789786, Length=342, Percent_Identity=41.5204678362573, Blast_Score=276, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI71982800, Length=347, Percent_Identity=34.2939481268012, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71982793, Length=347, Percent_Identity=34.2939481268012, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6320474, Length=361, Percent_Identity=38.2271468144044, Blast_Score=218, Evalue=1e-57,
Organism=Drosophila melanogaster, GI24666151, Length=343, Percent_Identity=39.067055393586, Blast_Score=231, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 38191; Mature: 38060

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITQRVFSGVQPTGNLHLGNYLGAIVNFVKLQETHNCIYCVVDLHAITVPVTVWGGPDE
CCCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEECCHHH
LRRNTREVTAAFIAAGIDPQKHIIFNQSQVAEHAELAWVFNCVARLGWLNRMTQFKEKAG
HHHHHHHHHHHHHHHCCCCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDRENASIGLYDYPVLMASDILVYRATHVPVGEDQKQHLELTRDIAQKFNNDFSASIAAQ
CCCCCCEECEECCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
GLGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADAIAQKIRKAKT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCC
DPEPLPTEEKGLEPRPEADNLVGIFAALAGKPKAQVLSEFGGGQFSAFKSNLVDLAVEKL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SPIAGEMKRLSADHGYVDSVLASGSDRARVIAAETMTAVKDIMGMVRKKG
HHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AITQRVFSGVQPTGNLHLGNYLGAIVNFVKLQETHNCIYCVVDLHAITVPVTVWGGPDE
CCHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEEEEEEECCHHH
LRRNTREVTAAFIAAGIDPQKHIIFNQSQVAEHAELAWVFNCVARLGWLNRMTQFKEKAG
HHHHHHHHHHHHHHHCCCCCHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDRENASIGLYDYPVLMASDILVYRATHVPVGEDQKQHLELTRDIAQKFNNDFSASIAAQ
CCCCCCEECEECCHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
GLGDAYFPLPEPVITGPATRVMSLRDGTKKMSKSDPSDYSRINLTDDADAIAQKIRKAKT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCC
DPEPLPTEEKGLEPRPEADNLVGIFAALAGKPKAQVLSEFGGGQFSAFKSNLVDLAVEKL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SPIAGEMKRLSADHGYVDSVLASGSDRARVIAAETMTAVKDIMGMVRKKG
HHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]