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The map label for this gene is gshB [H]

Identifier: 86747545

GI number: 86747545

Start: 462324

End: 463265

Strand: Reverse

Name: gshB [H]

Synonym: RPB_0419

Alternate gene names: 86747545

Gene position: 463265-462324 (Counterclockwise)

Preceding gene: 86747546

Following gene: 86747544

Centisome position: 8.69

GC content: 65.39

Gene sequence:

>942_bases
ATGACATTGAAGGTCGCCGTCCAGATGGACCCCATCGCGCGGATCAACATCCGCGGCGATTCCACATTCGCGCTGCTGCT
CGAAGCACAGAAGCGCGGCCATGCGATCTCGTACTTCACCCCCGACAAACTCTCGCTGCGCGGGACCGACGTTGTGGCCT
CCGCGCAAAGCCTGAGCGTCCGCGATCTTGAGGGCGATCACTTCACGCTGGGCGAGCCCGAACGCATCGACATGCGCAGC
TTCGACGCGGTGCTGCTGCGCCAGGACCCGCCGTTCGACCTGGCTTACATCACCAGCACCCATCTGCTGGAACGGATTCA
TCCGCAGACGCTGGTCGTCAATGATCCGGCCAGCGTCCGCAACGCGCCGGAAAAGCTGTTCGTGATGGACTTCGCCGAGC
TGATGCCGCCGACGCTGATCAGCCGCAATCTCGACGAGATCAATGCGTTTCGCGCCGAGCACGGCGCCGTGGTGATGAAG
CCGCTGCACGGCCATGGCGGCGCCGCGGTGTTCCGCGTGCTGCCGCAGGACATCAATTTCGGCTCGCTGTACGACATGTT
CGCCGTCACCTTCCGCGAACCCTGGGTGATCCAGCGCTTCCTCCCCGAGGTGAAGCACGGTGACAAGCGCATCATCCTGG
TCGACGGCGAATTCGCCGGCGCGGTCAACCGGGTGCCTGCGGCCGACGATCTGCGCTCCAACATGGTGCGCGGCGGAGCC
GCGGCGGCGACCGATCTGTCGGAGCGCGAGCGCGCCATCTGCGCGACGCTCGGCCCCAAGCTGCGCGAGCGCGGGTTGTT
GCTGGTCGGAATCGACGTGATCGATGGTTATCTCACCGAAATCAACGTCACCTCGCCGACCGGCATTCGCGCCGTGGCGC
GGCTCGGCGGCCCGGACATCGCCGCCCGGGTCTGGGACGTGATTGAGCAAAAGCGATCATGA

Upstream 100 bases:

>100_bases
TCGACGCGAGCCCTTGACCCGCCGGTCCTGATCGCCGGTCCTTGACCGCGGCATCCGATCGGCCGATAGCCCTTTCCGCC
CGCAACCCTGGAAGCTCTGC

Downstream 100 bases:

>100_bases
ACCGGACGTTTCGCCGCGGCCCTGTTTGACGTTAGTATCGATTGTCCCCTCTTGCTAGTTCGGTGACGCCGCTCCAGAAG
TAGTGGGGTGCGGGCGGAGC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTLKVAVQMDPIARINIRGDSTFALLLEAQKRGHAISYFTPDKLSLRGTDVVASAQSLSVRDLEGDHFTLGEPERIDMRS
FDAVLLRQDPPFDLAYITSTHLLERIHPQTLVVNDPASVRNAPEKLFVMDFAELMPPTLISRNLDEINAFRAEHGAVVMK
PLHGHGGAAVFRVLPQDINFGSLYDMFAVTFREPWVIQRFLPEVKHGDKRIILVDGEFAGAVNRVPAADDLRSNMVRGGA
AAATDLSERERAICATLGPKLRERGLLLVGIDVIDGYLTEINVTSPTGIRAVARLGGPDIAARVWDVIEQKRS

Sequences:

>Translated_313_residues
MTLKVAVQMDPIARINIRGDSTFALLLEAQKRGHAISYFTPDKLSLRGTDVVASAQSLSVRDLEGDHFTLGEPERIDMRS
FDAVLLRQDPPFDLAYITSTHLLERIHPQTLVVNDPASVRNAPEKLFVMDFAELMPPTLISRNLDEINAFRAEHGAVVMK
PLHGHGGAAVFRVLPQDINFGSLYDMFAVTFREPWVIQRFLPEVKHGDKRIILVDGEFAGAVNRVPAADDLRSNMVRGGA
AAATDLSERERAICATLGPKLRERGLLLVGIDVIDGYLTEINVTSPTGIRAVARLGGPDIAARVWDVIEQKRS
>Mature_312_residues
TLKVAVQMDPIARINIRGDSTFALLLEAQKRGHAISYFTPDKLSLRGTDVVASAQSLSVRDLEGDHFTLGEPERIDMRSF
DAVLLRQDPPFDLAYITSTHLLERIHPQTLVVNDPASVRNAPEKLFVMDFAELMPPTLISRNLDEINAFRAEHGAVVMKP
LHGHGGAAVFRVLPQDINFGSLYDMFAVTFREPWVIQRFLPEVKHGDKRIILVDGEFAGAVNRVPAADDLRSNMVRGGAA
AATDLSERERAICATLGPKLRERGLLLVGIDVIDGYLTEINVTSPTGIRAVARLGGPDIAARVWDVIEQKRS

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=311, Percent_Identity=38.9067524115756, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34508; Mature: 34377

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKVAVQMDPIARINIRGDSTFALLLEAQKRGHAISYFTPDKLSLRGTDVVASAQSLSV
CEEEEEEEECCEEEEEECCCCEEEEEEEHHHCCCEEEEECCCCEEECCCHHEECCCCCEE
RDLEGDHFTLGEPERIDMRSFDAVLLRQDPPFDLAYITSTHLLERIHPQTLVVNDPASVR
EECCCCEEECCCCCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCHHHC
NAPEKLFVMDFAELMPPTLISRNLDEINAFRAEHGAVVMKPLHGHGGAAVFRVLPQDINF
CCCHHHHEEEHHHHCCHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCC
GSLYDMFAVTFREPWVIQRFLPEVKHGDKRIILVDGEFAGAVNRVPAADDLRSNMVRGGA
CHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCC
AAATDLSERERAICATLGPKLRERGLLLVGIDVIDGYLTEINVTSPTGIRAVARLGGPDI
HHHCCHHHHHHHHHHHCCHHHHHCCEEEEEEEHHCCEEEEEEECCCCHHHHHHHCCCCHH
AARVWDVIEQKRS
HHHHHHHHHHCCC
>Mature Secondary Structure 
TLKVAVQMDPIARINIRGDSTFALLLEAQKRGHAISYFTPDKLSLRGTDVVASAQSLSV
EEEEEEEECCEEEEEECCCCEEEEEEEHHHCCCEEEEECCCCEEECCCHHEECCCCCEE
RDLEGDHFTLGEPERIDMRSFDAVLLRQDPPFDLAYITSTHLLERIHPQTLVVNDPASVR
EECCCCEEECCCCCCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHCCCCEEEEECCHHHC
NAPEKLFVMDFAELMPPTLISRNLDEINAFRAEHGAVVMKPLHGHGGAAVFRVLPQDINF
CCCHHHHEEEHHHHCCHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCC
GSLYDMFAVTFREPWVIQRFLPEVKHGDKRIILVDGEFAGAVNRVPAADDLRSNMVRGGA
CHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCC
AAATDLSERERAICATLGPKLRERGLLLVGIDVIDGYLTEINVTSPTGIRAVARLGGPDI
HHHCCHHHHHHHHHHHCCHHHHHCCEEEEEEEHHCCEEEEEEECCCCHHHHHHHCCCCHH
AARVWDVIEQKRS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA