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The map label for this gene is nodV [H]

Identifier: 86747340

GI number: 86747340

Start: 240693

End: 241976

Strand: Reverse

Name: nodV [H]

Synonym: RPB_0214

Alternate gene names: 86747340

Gene position: 241976-240693 (Counterclockwise)

Preceding gene: 86747342

Following gene: 86747339

Centisome position: 4.54

GC content: 62.46

Gene sequence:

>1284_bases
GTGCCGCTCAGCCTCTCCGACAGTTTCGATCTGGCCGCCGTGGTAAAAACCTCTGAGGCGGTCTCGAGCGAACTGGGCGT
TCCGCGCCTGATGGAAACGCTGATGACGCTGGTGCTGGAACATGCTGGCGCGCAGCGCGGCCTGCTGATCCTCCCGCAGC
AGGCCGATTTGACGATCCGCACCGAGGCGAGCACCGGCGACGACGGCATTCAAGTCAAGCTGCGCGACGACATCGCCACC
GAAGACGATCTGCCGACCTCGCTGGTGAACCTGGCCGCGCGAACGGGTGAAGCGATTACGGTCGACGACGCCCAATCCAG
CCCGTTCGGGGACGATCCGTATATCGCACGGATGAAGTCACGCTCGCTGCTGTGCCTACCGCTGATGAAGCGCAACAAGT
TGCTCGCGATCATCTTCCTCGAGAACGATCTCGCCACGGGCGTGTTCACGCCGATGAAGTTATCGGTTCTGAAGTTGCTG
TCGTCGCAGGCGGCGATCTCCCTCAAGAACGCCCTCCTGGAAGAAAAGGACGCACTGGTCGAGGCGTTGCAAAAATCGCA
ATCCGAGCTGATGCGGATGTCTCGCCTGACTGCAATCGGCGAACTGGTGATTTCGATCGCGCACGAGATCAACCAGCCGC
TGACGGCGATCATCACCAATGCCGACGTGTACCTGCGCTGGCTGGGCAACGAGCAACCGTCGATCGTCGAGGCCGAGAGC
GCCGCTCGCCGCGTCATCGAGAACGGCCGACGCGCCGCAGAGGTGGTGCAGACGATCCGCGGCCTGGCGGTCAAGTCCGT
GCCGAAGATGTTGCCAGTCGACGTCAACGCCGTCGTCCAGGAAATCCTTTCGATCGTTCAGAGCGAATTGCGTCAACATA
CGATCCACCTCGAAACCAGACTGGCGGACGGTGCCGGCGTCGTCATCGGCAACAAGGTACAGCTGCAGCAGGTGCTACTC
AATTTGACCATGGACGCGATCGAGTCCATGGCCGCGATCGAGGGCCGCCGGCTGCTGACGGTGACCACCGCGTCGGGTGA
CGCCGACAGTATTGTCGTCACCCTCGAGGACAACGGCCTCGGTTTCCACGAAGGCCGCATCGAGCAGCTGTTCGAAGCGC
TGGTGACGACCAAGCCCCAGGGCATGGGCATGGGACTGTCGATTAGCCGATCGATCGTGGAAGCGCATGGAGGGCGGCTA
TGGGCGAGATCGATCAAGCCGCACGGGGCACGCTTTCAGTTTTCCCTTCCGCGTTCGGCGGAGGCCGACGCGGCGGCCAT
GTAG

Upstream 100 bases:

>100_bases
CATAAGGTGGGCGGAGGCTGCAACCCGTTTCGTCGAGCCTCTCTGGCCCCAGTCTTCGGCAAGTAGCGATCCCCTAGTGT
CGCCGGCTTAAGGTACGCAG

Downstream 100 bases:

>100_bases
TCGGAATGTTCCGCGACGTGATGTCGCGAGCAGATTGATCGCTTGGGACAAGCAACCCAACCGGACCGCATTGCGCCCGA
AAATCGGCTTCGCCCACGCA

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPLSLSDSFDLAAVVKTSEAVSSELGVPRLMETLMTLVLEHAGAQRGLLILPQQADLTIRTEASTGDDGIQVKLRDDIAT
EDDLPTSLVNLAARTGEAITVDDAQSSPFGDDPYIARMKSRSLLCLPLMKRNKLLAIIFLENDLATGVFTPMKLSVLKLL
SSQAAISLKNALLEEKDALVEALQKSQSELMRMSRLTAIGELVISIAHEINQPLTAIITNADVYLRWLGNEQPSIVEAES
AARRVIENGRRAAEVVQTIRGLAVKSVPKMLPVDVNAVVQEILSIVQSELRQHTIHLETRLADGAGVVIGNKVQLQQVLL
NLTMDAIESMAAIEGRRLLTVTTASGDADSIVVTLEDNGLGFHEGRIEQLFEALVTTKPQGMGMGLSISRSIVEAHGGRL
WARSIKPHGARFQFSLPRSAEADAAAM

Sequences:

>Translated_427_residues
MPLSLSDSFDLAAVVKTSEAVSSELGVPRLMETLMTLVLEHAGAQRGLLILPQQADLTIRTEASTGDDGIQVKLRDDIAT
EDDLPTSLVNLAARTGEAITVDDAQSSPFGDDPYIARMKSRSLLCLPLMKRNKLLAIIFLENDLATGVFTPMKLSVLKLL
SSQAAISLKNALLEEKDALVEALQKSQSELMRMSRLTAIGELVISIAHEINQPLTAIITNADVYLRWLGNEQPSIVEAES
AARRVIENGRRAAEVVQTIRGLAVKSVPKMLPVDVNAVVQEILSIVQSELRQHTIHLETRLADGAGVVIGNKVQLQQVLL
NLTMDAIESMAAIEGRRLLTVTTASGDADSIVVTLEDNGLGFHEGRIEQLFEALVTTKPQGMGMGLSISRSIVEAHGGRL
WARSIKPHGARFQFSLPRSAEADAAAM
>Mature_426_residues
PLSLSDSFDLAAVVKTSEAVSSELGVPRLMETLMTLVLEHAGAQRGLLILPQQADLTIRTEASTGDDGIQVKLRDDIATE
DDLPTSLVNLAARTGEAITVDDAQSSPFGDDPYIARMKSRSLLCLPLMKRNKLLAIIFLENDLATGVFTPMKLSVLKLLS
SQAAISLKNALLEEKDALVEALQKSQSELMRMSRLTAIGELVISIAHEINQPLTAIITNADVYLRWLGNEQPSIVEAESA
ARRVIENGRRAAEVVQTIRGLAVKSVPKMLPVDVNAVVQEILSIVQSELRQHTIHLETRLADGAGVVIGNKVQLQQVLLN
LTMDAIESMAAIEGRRLLTVTTASGDADSIVVTLEDNGLGFHEGRIEQLFEALVTTKPQGMGMGLSISRSIVEAHGGRLW
ARSIKPHGARFQFSLPRSAEADAAAM

Specific function: Member of the two-component regulatory system nodV/nodW probably involved in the regulation of the transcription of genes involved in the nodulation process. NodV may function as a membrane-associated protein kinase that phosphorylates nodW in response to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=27.2727272727273, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI87081816, Length=259, Percent_Identity=23.9382239382239, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46116; Mature: 45985

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSLSDSFDLAAVVKTSEAVSSELGVPRLMETLMTLVLEHAGAQRGLLILPQQADLTIR
CCCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEE
TEASTGDDGIQVKLRDDIATEDDLPTSLVNLAARTGEAITVDDAQSSPFGDDPYIARMKS
ECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCC
RSLLCLPLMKRNKLLAIIFLENDLATGVFTPMKLSVLKLLSSQAAISLKNALLEEKDALV
CCEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALQKSQSELMRMSRLTAIGELVISIAHEINQPLTAIITNADVYLRWLGNEQPSIVEAES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCEEEHHH
AARRVIENGRRAAEVVQTIRGLAVKSVPKMLPVDVNAVVQEILSIVQSELRQHTIHLETR
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEE
LADGAGVVIGNKVQLQQVLLNLTMDAIESMAAIEGRRLLTVTTASGDADSIVVTLEDNGL
ECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCC
GFHEGRIEQLFEALVTTKPQGMGMGLSISRSIVEAHGGRLWARSIKPHGARFQFSLPRSA
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCC
EADAAAM
CCCCCCC
>Mature Secondary Structure 
PLSLSDSFDLAAVVKTSEAVSSELGVPRLMETLMTLVLEHAGAQRGLLILPQQADLTIR
CCCCCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEE
TEASTGDDGIQVKLRDDIATEDDLPTSLVNLAARTGEAITVDDAQSSPFGDDPYIARMKS
ECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHCC
RSLLCLPLMKRNKLLAIIFLENDLATGVFTPMKLSVLKLLSSQAAISLKNALLEEKDALV
CCEEEEECCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALQKSQSELMRMSRLTAIGELVISIAHEINQPLTAIITNADVYLRWLGNEQPSIVEAES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEECCCCCCEEEHHH
AARRVIENGRRAAEVVQTIRGLAVKSVPKMLPVDVNAVVQEILSIVQSELRQHTIHLETR
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEE
LADGAGVVIGNKVQLQQVLLNLTMDAIESMAAIEGRRLLTVTTASGDADSIVVTLEDNGL
ECCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCC
GFHEGRIEQLFEALVTTKPQGMGMGLSISRSIVEAHGGRLWARSIKPHGARFQFSLPRSA
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCCC
EADAAAM
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2320582; 11157954; 12597275 [H]