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The map label for this gene is queA [H]

Identifier: 86609129

GI number: 86609129

Start: 1747081

End: 1748259

Strand: Reverse

Name: queA [H]

Synonym: CYB_1668

Alternate gene names: 86609129

Gene position: 1748259-1747081 (Counterclockwise)

Preceding gene: 86609130

Following gene: 86609124

Centisome position: 57.38

GC content: 62.34

Gene sequence:

>1179_bases
ATGCCTGACTCTTTTTCGCCTGCTGCTGCTCCCCCCGGTGAGGCTGAGCCTTTGGGCGATAGGGAGTCCAATCCCGATCT
GCTTACAGCTTCCTACGATTACACTCTCCCTCCAGACCAGATTGCGCAAACGCCGGTGGAGCCCAGGGATCAGGCGCGGA
TGCTGGTGGTGTACCCGGATAGGCACGAGCATCGCCGCTTCTGCCATCTGCCCGACTACCTGCGGGAGGGGGATCTGCTG
GTTCTCAACGATACCAAGGTGATCCCGGCCCGCCTGTTCGGGGCCAAGGAACCCTCCGGGGCGCGGGTCGAAGTGTTGCT
GCTGGAGCCTTGTCTGGAAGGGATCCCTGCCGCTGCGCAACCTCTGCCGGATCAGGCGTGGAGCTGGCGGGTTTTGGTCA
GGCCGGGCAAACGGGTGAAGGTGGGGGATCGGATTGGTTTTTGGAGCAAGCAGGGATCCCCCTCTGGGCTGAGGGCCACT
GTTCTGGCTTTGGACCCGGAAACCGGCGGGCGAGTGGTGCAGTTTGAGTGGGAAGGGGCGCAACCTTTTGAGGAAATCCT
GGAACAGGTGGGCCGGATCCCGCTTCCTCCCTACATCAAGGCTTCCGGATCCCCGGGCTCAGGACAAGTTCGCCCTGAAG
ACTACCAAACCCTCTGGGCCAAACACCCCGGCTCGGTGGCCGCTCCCACGGCAGGGCTGCACTTTACCCAGGAGCTGCTG
GAGCGGCTGGCCCAAAAAGGGATCCAAACGGCCTACATCACCCTGAACATCGGCCTGGGTACCTTCCGACCGGTGGAAGC
GGAGCGGGTAACCGAGCATCGTCTCCACAGCGAGTGGCTCAAGGTGCCGGCAGAAACGGTGGCAGCCATCCAAAAAGCCC
AAGCCCGAGGGGGGCGGGTGATCGCGGTGGGAACGACTGTGGTGCGCTCTTTGGAAACCGCGGCAGCCTCTGGCACCTTG
CAGCCTTGGCAGGGGAAAAGCGATCTATTCATCTACCCCGGCTACTCCTGGCGGGTGATTCAAGGACTGGTCACCAATTT
TCATCTGCCCCGCTCCAGCCTGATGATGCTGGTGGCTGCGCTGATCGGGCGGCAAAGGCTGCTGGATCTCTACCGAGAGG
CCGTCGACCAGGGGTATCGCTTCTATTCCTTTGGGGATGGCATGCTGATCCTGCCCTAG

Upstream 100 bases:

>100_bases
TGGTGGCCAAGGTTTAGCTGCTTCCCTCAAAAGGGAGAGGGTTGGCGGGATCCGGTTGGCTATGCTGGATCCACGTAGAA
CCCCATCTTGATTCATCTCC

Downstream 100 bases:

>100_bases
GGCTGGGGCTGCTCTCCCTGGGATCTCTTGCAGGAACCGGCCTCTGCTTCTGGGGTCGAAGGACGGTCTTGGCCGCCGGC
AAACCAATTCTGGATGCGCT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPDSFSPAAAPPGEAEPLGDRESNPDLLTASYDYTLPPDQIAQTPVEPRDQARMLVVYPDRHEHRRFCHLPDYLREGDLL
VLNDTKVIPARLFGAKEPSGARVEVLLLEPCLEGIPAAAQPLPDQAWSWRVLVRPGKRVKVGDRIGFWSKQGSPSGLRAT
VLALDPETGGRVVQFEWEGAQPFEEILEQVGRIPLPPYIKASGSPGSGQVRPEDYQTLWAKHPGSVAAPTAGLHFTQELL
ERLAQKGIQTAYITLNIGLGTFRPVEAERVTEHRLHSEWLKVPAETVAAIQKAQARGGRVIAVGTTVVRSLETAAASGTL
QPWQGKSDLFIYPGYSWRVIQGLVTNFHLPRSSLMMLVAALIGRQRLLDLYREAVDQGYRFYSFGDGMLILP

Sequences:

>Translated_392_residues
MPDSFSPAAAPPGEAEPLGDRESNPDLLTASYDYTLPPDQIAQTPVEPRDQARMLVVYPDRHEHRRFCHLPDYLREGDLL
VLNDTKVIPARLFGAKEPSGARVEVLLLEPCLEGIPAAAQPLPDQAWSWRVLVRPGKRVKVGDRIGFWSKQGSPSGLRAT
VLALDPETGGRVVQFEWEGAQPFEEILEQVGRIPLPPYIKASGSPGSGQVRPEDYQTLWAKHPGSVAAPTAGLHFTQELL
ERLAQKGIQTAYITLNIGLGTFRPVEAERVTEHRLHSEWLKVPAETVAAIQKAQARGGRVIAVGTTVVRSLETAAASGTL
QPWQGKSDLFIYPGYSWRVIQGLVTNFHLPRSSLMMLVAALIGRQRLLDLYREAVDQGYRFYSFGDGMLILP
>Mature_391_residues
PDSFSPAAAPPGEAEPLGDRESNPDLLTASYDYTLPPDQIAQTPVEPRDQARMLVVYPDRHEHRRFCHLPDYLREGDLLV
LNDTKVIPARLFGAKEPSGARVEVLLLEPCLEGIPAAAQPLPDQAWSWRVLVRPGKRVKVGDRIGFWSKQGSPSGLRATV
LALDPETGGRVVQFEWEGAQPFEEILEQVGRIPLPPYIKASGSPGSGQVRPEDYQTLWAKHPGSVAAPTAGLHFTQELLE
RLAQKGIQTAYITLNIGLGTFRPVEAERVTEHRLHSEWLKVPAETVAAIQKAQARGGRVIAVGTTVVRSLETAAASGTLQ
PWQGKSDLFIYPGYSWRVIQGLVTNFHLPRSSLMMLVAALIGRQRLLDLYREAVDQGYRFYSFGDGMLILP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=365, Percent_Identity=41.0958904109589, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 43246; Mature: 43115

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDSFSPAAAPPGEAEPLGDRESNPDLLTASYDYTLPPDQIAQTPVEPRDQARMLVVYPD
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCEEEEECCC
RHEHRRFCHLPDYLREGDLLVLNDTKVIPARLFGAKEPSGARVEVLLLEPCLEGIPAAAQ
CCCCCCCCCCCHHHCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEEECHHHHCCCCCCC
PLPDQAWSWRVLVRPGKRVKVGDRIGFWSKQGSPSGLRATVLALDPETGGRVVQFEWEGA
CCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCC
QPFEEILEQVGRIPLPPYIKASGSPGSGQVRPEDYQTLWAKHPGSVAAPTAGLHFTQELL
CHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHH
ERLAQKGIQTAYITLNIGLGTFRPVEAERVTEHRLHSEWLKVPAETVAAIQKAQARGGRV
HHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEE
IAVGTTVVRSLETAAASGTLQPWQGKSDLFIYPGYSWRVIQGLVTNFHLPRSSLMMLVAA
EEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHH
LIGRQRLLDLYREAVDQGYRFYSFGDGMLILP
HHHHHHHHHHHHHHHHCCCEEEEECCCEEECC
>Mature Secondary Structure 
PDSFSPAAAPPGEAEPLGDRESNPDLLTASYDYTLPPDQIAQTPVEPRDQARMLVVYPD
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCEEEEECCC
RHEHRRFCHLPDYLREGDLLVLNDTKVIPARLFGAKEPSGARVEVLLLEPCLEGIPAAAQ
CCCCCCCCCCCHHHCCCCEEEEECCCCCHHHHHCCCCCCCCEEEEEEECHHHHCCCCCCC
PLPDQAWSWRVLVRPGKRVKVGDRIGFWSKQGSPSGLRATVLALDPETGGRVVQFEWEGA
CCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCC
QPFEEILEQVGRIPLPPYIKASGSPGSGQVRPEDYQTLWAKHPGSVAAPTAGLHFTQELL
CHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHH
ERLAQKGIQTAYITLNIGLGTFRPVEAERVTEHRLHSEWLKVPAETVAAIQKAQARGGRV
HHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEE
IAVGTTVVRSLETAAASGTLQPWQGKSDLFIYPGYSWRVIQGLVTNFHLPRSSLMMLVAA
EEEHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHH
LIGRQRLLDLYREAVDQGYRFYSFGDGMLILP
HHHHHHHHHHHHHHHHCCCEEEEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA