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The map label for this gene is ksgA

Identifier: 86608465

GI number: 86608465

Start: 1021057

End: 1021905

Strand: Reverse

Name: ksgA

Synonym: CYB_0986

Alternate gene names: 86608465

Gene position: 1021905-1021057 (Counterclockwise)

Preceding gene: 86608466

Following gene: 86608464

Centisome position: 33.54

GC content: 63.72

Gene sequence:

>849_bases
TTGCCCTATCCTCGTAAACGGTTTGGTCAGCATTGGCTCAAGGATCCCGCTGTTCACGAGGCGATTTTGCGGGCTGCCCA
GTTGAATGATCTGGAGAGAGGGGCGGATCCCACTTGGGTGCTGGAGATCGGCCCCGGCACCGGCCAACTGACGCGGCGAT
TGCTGGCCCAGGGTGTCCAGGTGGTGGCGGTGGAGATCGACCGGGATCTGTGCCGACTGCTGCGAAAGCGCTTCGCCGAC
CAGCCCCGCTTCCACCTGGTGGAAGGGGATTTTCTTCGCCTGCCGTTGCCCCCTCAGCCTCGCCTGCTGGTGGCCAACAT
TCCCTACAACCTCACCGGATCCATCCTGGAAAAGGTACTGGGATCCCCGGCCCAGCCGGTTCGGCAGTTCGAGCGCATTG
TCCTTCTGGTGCAAAAAGAGCTGGCCGAGCGCTTGCAGGCCGGCCCCGGCAGCAAAGCCTATGGTGCCCTCAGCCTGCGG
ACGCAGTATTTGGCCGATTGCGAGCTGATCTGCAGGGTGCCCCCCACCGCTTTCAAGCCCGCCCCCAAGGTGGAATCTGC
TGTGATTCGCCTCACCCCCCGCCCCGCCCCCACCCCCGTCCGGGATCCCTGCTGGTTTAACCACCTCCTTCGACAGGGGT
TCTCCACCCGTCGCAAGAAGCTCGTCAATGCCCTGGGAAGCTTGGTGGAGCGAGAGGTGGTCGCAGCAGCTTTGGCTCAG
CTCCGCCTCAACCCCGATGCCCGCGCCGAAGAGCTGGATCTGCCCCACTGGCTGGCCTTGAGCGACCTGCTGCTGGAGAA
AGCGCCCAAAAGGGCTGTTGTTCCTCAAGAGGAGCAGGAACCAGGGTAG

Upstream 100 bases:

>100_bases
GACCTGAGGATGCGGATCCCACCTTGATTTCTGTTCCCAACTCAGTCCAGCGATGACCCACCCGGCCATTTCTCCTTCAA
CCGCTCTGGGTACCGCTCGG

Downstream 100 bases:

>100_bases
GGTAAGAGAGGGAAGCTCAGTTGGGCGAACCCAAACATCCTCTGGGGTTTTTGAAGTCCCTTCCCGATCTTGTTCTGGAT
CCCTTTCGTCAATGCAGGTG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPYPRKRFGQHWLKDPAVHEAILRAAQLNDLERGADPTWVLEIGPGTGQLTRRLLAQGVQVVAVEIDRDLCRLLRKRFAD
QPRFHLVEGDFLRLPLPPQPRLLVANIPYNLTGSILEKVLGSPAQPVRQFERIVLLVQKELAERLQAGPGSKAYGALSLR
TQYLADCELICRVPPTAFKPAPKVESAVIRLTPRPAPTPVRDPCWFNHLLRQGFSTRRKKLVNALGSLVEREVVAAALAQ
LRLNPDARAEELDLPHWLALSDLLLEKAPKRAVVPQEEQEPG

Sequences:

>Translated_282_residues
MPYPRKRFGQHWLKDPAVHEAILRAAQLNDLERGADPTWVLEIGPGTGQLTRRLLAQGVQVVAVEIDRDLCRLLRKRFAD
QPRFHLVEGDFLRLPLPPQPRLLVANIPYNLTGSILEKVLGSPAQPVRQFERIVLLVQKELAERLQAGPGSKAYGALSLR
TQYLADCELICRVPPTAFKPAPKVESAVIRLTPRPAPTPVRDPCWFNHLLRQGFSTRRKKLVNALGSLVEREVVAAALAQ
LRLNPDARAEELDLPHWLALSDLLLEKAPKRAVVPQEEQEPG
>Mature_281_residues
PYPRKRFGQHWLKDPAVHEAILRAAQLNDLERGADPTWVLEIGPGTGQLTRRLLAQGVQVVAVEIDRDLCRLLRKRFADQ
PRFHLVEGDFLRLPLPPQPRLLVANIPYNLTGSILEKVLGSPAQPVRQFERIVLLVQKELAERLQAGPGSKAYGALSLRT
QYLADCELICRVPPTAFKPAPKVESAVIRLTPRPAPTPVRDPCWFNHLLRQGFSTRRKKLVNALGSLVEREVVAAALAQL
RLNPDARAEELDLPHWLALSDLLLEKAPKRAVVPQEEQEPG

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=223, Percent_Identity=38.1165919282511, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI156415992, Length=283, Percent_Identity=26.5017667844523, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1786236, Length=272, Percent_Identity=32.7205882352941, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25146882, Length=217, Percent_Identity=37.7880184331797, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25141369, Length=296, Percent_Identity=24.6621621621622, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324989, Length=221, Percent_Identity=36.6515837104072, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21358017, Length=219, Percent_Identity=42.0091324200913, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI21357273, Length=287, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_SYNJB (Q2JMR8)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477227.1
- ProteinModelPortal:   Q2JMR8
- SMR:   Q2JMR8
- STRING:   Q2JMR8
- GeneID:   3900296
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_0986
- TIGR:   CYB_0986
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- PhylomeDB:   Q2JMR8
- ProtClustDB:   PRK00274
- BioCyc:   SSP321332:CYB_0986-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31611; Mature: 31480

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 11-11 BINDING 13-13 BINDING 44-44 BINDING 65-65 BINDING 90-90 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYPRKRFGQHWLKDPAVHEAILRAAQLNDLERGADPTWVLEIGPGTGQLTRRLLAQGVQ
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCE
VVAVEIDRDLCRLLRKRFADQPRFHLVEGDFLRLPLPPQPRLLVANIPYNLTGSILEKVL
EEEEECCHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCEEEEECCCCCCHHHHHHHH
GSPAQPVRQFERIVLLVQKELAERLQAGPGSKAYGALSLRTQYLADCELICRVPPTAFKP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHEECCCCCCCCC
APKVESAVIRLTPRPAPTPVRDPCWFNHLLRQGFSTRRKKLVNALGSLVEREVVAAALAQ
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLNPDARAEELDLPHWLALSDLLLEKAPKRAVVPQEEQEPG
HHCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PYPRKRFGQHWLKDPAVHEAILRAAQLNDLERGADPTWVLEIGPGTGQLTRRLLAQGVQ
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCE
VVAVEIDRDLCRLLRKRFADQPRFHLVEGDFLRLPLPPQPRLLVANIPYNLTGSILEKVL
EEEEECCHHHHHHHHHHCCCCCCEEEECCCEEECCCCCCCCEEEEECCCCCCHHHHHHHH
GSPAQPVRQFERIVLLVQKELAERLQAGPGSKAYGALSLRTQYLADCELICRVPPTAFKP
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHEECCCCCCCCC
APKVESAVIRLTPRPAPTPVRDPCWFNHLLRQGFSTRRKKLVNALGSLVEREVVAAALAQ
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRLNPDARAEELDLPHWLALSDLLLEKAPKRAVVPQEEQEPG
HHCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA