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The map label for this gene is gntR [H]

Identifier: 86361074

GI number: 86361074

Start: 385641

End: 386510

Strand: Reverse

Name: gntR [H]

Synonym: RHE_PF00344

Alternate gene names: 86361074

Gene position: 386510-385641 (Counterclockwise)

Preceding gene: 86361085

Following gene: 86361073

Centisome position: 60.16

GC content: 63.56

Gene sequence:

>870_bases
ATGGCCGCCGTGGAACGGCTGGGCTATGTGCCGAACCGCATCGCCGGAACGCTCGCTTCCACCACGTCACGCCTTGTCGC
CTTCGTCATTCCCTCCCTGTCCAACATCGTCTTTGCCGACGTGCTGCGCGGTGCCGGCGCTGTCCTGGAGGAAAACCGAT
ATCAGGCGGTCTTCTCGGTGACCGATTATGATCCGAGCAAGGAGGAGGCGCTCGCCGCTGCGATGCTTGCCTGGCGGCCG
GCAGCGGTGATGCTGGCGGGATATGAGCATACCGACGGCACGGCGAAGATGCTGCGCGCCAGCGGTTGCCGGGTGGTGGA
ATTGCTTGATCTCGACGGCGATGCGATCGATATCGCGGTCGGTTTCTCGAACCGTGCGGCCGGGCGGGAGAGCGCGGCAT
TCCTGCTCAAGCGAGGCTATCGCCGGATCGGCTATGTCGGCCACGATCTCAGCCGCGACATCCGCGCCGGCAAGCGCTTT
GCCAGCTTTTGCGAAATTCTCGAAGCCAACGGCACGCCCCTTGTCGCCCGCGAAATTCTGGCGGGCGCTTCGTCGGTGGA
GAACGGCAGGCTGGCGCTGGAGCGACTGCTTGCACGAGCGAGCGATCTCGATGCGGTCTATTTTTCCAACGACGACATGG
CGCTCGGCGGTTATTTTCACTGTCTCGCCCAAGGAATTGTCATTCCCTCGCAGCTGGCTCTTTTTGGCTATAACGGTCTC
GATATCGGCCGGGCGATACCGCAGCCCTTGTCGACCATCCGGACGCCGCGCGTCGCGACGGGGCAGATGGCCGCCGAGCT
GGTTGTCGCCAACGCGCCGCCGCAGGCTATCGACCTCGGTTTTGAACTGATCGAAGGGGCGACTGCGTAA

Upstream 100 bases:

>100_bases
CCTTGCAGAGGTCGCAGAAGCGGCCGGCGTCGGGGAGAGCACGGTGTCGCGTGTCCTGCGCAACCATGGTTCGTTTTCGG
GCAAGACACGGGAGCGGGTG

Downstream 100 bases:

>100_bases
CTTTGGAGGAACTGTCATGTCCGACGCGCGCCTGCGTGAGGAAATCTGCCGGTACGGCCGCTCGCTGTTCGAGCGCGGGC
TGACGCCCGGTTCGTCCGGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAAVERLGYVPNRIAGTLASTTSRLVAFVIPSLSNIVFADVLRGAGAVLEENRYQAVFSVTDYDPSKEEALAAAMLAWRP
AAVMLAGYEHTDGTAKMLRASGCRVVELLDLDGDAIDIAVGFSNRAAGRESAAFLLKRGYRRIGYVGHDLSRDIRAGKRF
ASFCEILEANGTPLVAREILAGASSVENGRLALERLLARASDLDAVYFSNDDMALGGYFHCLAQGIVIPSQLALFGYNGL
DIGRAIPQPLSTIRTPRVATGQMAAELVVANAPPQAIDLGFELIEGATA

Sequences:

>Translated_289_residues
MAAVERLGYVPNRIAGTLASTTSRLVAFVIPSLSNIVFADVLRGAGAVLEENRYQAVFSVTDYDPSKEEALAAAMLAWRP
AAVMLAGYEHTDGTAKMLRASGCRVVELLDLDGDAIDIAVGFSNRAAGRESAAFLLKRGYRRIGYVGHDLSRDIRAGKRF
ASFCEILEANGTPLVAREILAGASSVENGRLALERLLARASDLDAVYFSNDDMALGGYFHCLAQGIVIPSQLALFGYNGL
DIGRAIPQPLSTIRTPRVATGQMAAELVVANAPPQAIDLGFELIEGATA
>Mature_288_residues
AAVERLGYVPNRIAGTLASTTSRLVAFVIPSLSNIVFADVLRGAGAVLEENRYQAVFSVTDYDPSKEEALAAAMLAWRPA
AVMLAGYEHTDGTAKMLRASGCRVVELLDLDGDAIDIAVGFSNRAAGRESAAFLLKRGYRRIGYVGHDLSRDIRAGKRFA
SFCEILEANGTPLVAREILAGASSVENGRLALERLLARASDLDAVYFSNDDMALGGYFHCLAQGIVIPSQLALFGYNGLD
IGRAIPQPLSTIRTPRVATGQMAAELVVANAPPQAIDLGFELIEGATA

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=294, Percent_Identity=35.7142857142857, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1790715, Length=292, Percent_Identity=32.5342465753425, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1788474, Length=274, Percent_Identity=24.8175182481752, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787948, Length=291, Percent_Identity=22.680412371134, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790369, Length=291, Percent_Identity=21.6494845360825, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790194, Length=271, Percent_Identity=21.0332103321033, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789068, Length=269, Percent_Identity=22.3048327137546, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789202, Length=268, Percent_Identity=24.2537313432836, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 30720; Mature: 30588

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVERLGYVPNRIAGTLASTTSRLVAFVIPSLSNIVFADVLRGAGAVLEENRYQAVFSV
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEE
TDYDPSKEEALAAAMLAWRPAAVMLAGYEHTDGTAKMLRASGCRVVELLDLDGDAIDIAV
ECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEE
GFSNRAAGRESAAFLLKRGYRRIGYVGHDLSRDIRAGKRFASFCEILEANGTPLVAREIL
ECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AGASSVENGRLALERLLARASDLDAVYFSNDDMALGGYFHCLAQGIVIPSQLALFGYNGL
HCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEHHHHHHHHHHCCCCCCHHHHCCCCCC
DIGRAIPQPLSTIRTPRVATGQMAAELVVANAPPQAIDLGFELIEGATA
CHHHHCCHHHHHHCCCCCCCHHHHHHEEEECCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AAVERLGYVPNRIAGTLASTTSRLVAFVIPSLSNIVFADVLRGAGAVLEENRYQAVFSV
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEE
TDYDPSKEEALAAAMLAWRPAAVMLAGYEHTDGTAKMLRASGCRVVELLDLDGDAIDIAV
ECCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCCEEEEEE
GFSNRAAGRESAAFLLKRGYRRIGYVGHDLSRDIRAGKRFASFCEILEANGTPLVAREIL
ECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AGASSVENGRLALERLLARASDLDAVYFSNDDMALGGYFHCLAQGIVIPSQLALFGYNGL
HCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEHHHHHHHHHHCCCCCCHHHHCCCCCC
DIGRAIPQPLSTIRTPRVATGQMAAELVVANAPPQAIDLGFELIEGATA
CHHHHCCHHHHHHCCCCCCCHHHHHHEEEECCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]