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The map label for this gene is prfB [H]

Identifier: 85860191

GI number: 85860191

Start: 2392998

End: 2393408

Strand: Direct

Name: prfB [H]

Synonym: SYN_00581

Alternate gene names: 85860191

Gene position: 2392998-2393408 (Clockwise)

Preceding gene: 85860190

Following gene: 85860192

Centisome position: 75.27

GC content: 54.26

Gene sequence:

>411_bases
ATGAGTATTTCACCGGAAAAAGAAAAAGACGTCGCCCAGCGGATGGCCCGCCTTGGAATTTCAGAGGACGATTTACGGGA
AACTTTTATCCGCTCTTCAGGACCCGGCGGTCAAAATGTCAACAAAACGGCAACCTGCGTCTATCTCGTTCACCTGCCCA
CGGGCCTTTCCGTTAAATGCCAGCAGGACCGCTCTCAAACCGTGAACCGTCTGCTGGCCAGGCGATTGCTTCTCGATCGG
ATCGAACGGCTGAAGACGGGGCGGATCGCCGCGGAGAAGGCGCGGATCGAAAAAATCCGCAGACAGAAAAGACGCCGCTC
GCAACGGGCGCAGGAAAAAGTCCTTGAGTTGAAGCATCGGCAGTCTGAAAGAAAGAAACTCCGGGAAAAAGTTGGAACAG
AGGATGCGTGA

Upstream 100 bases:

>100_bases
TTTGTCGTCACGATCGAACTGCTCCAGCGGTCCGTGGCCATCAAACTCGACAGTTCCCAGATCGAGAAGATATGAACCCC
GGGACGCGATATCCTTACAG

Downstream 100 bases:

>100_bases
CAGACATGGGGAGCAAAATCTCCCGACGGAAAATCAGAAGGAACGAGGGGAAAGGATAGTGCTGACACGGGATGAGCAGC
AAATCCTTCAAGCCCTGCAG

Product: peptide chain release factor

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSISPEKEKDVAQRMARLGISEDDLRETFIRSSGPGGQNVNKTATCVYLVHLPTGLSVKCQQDRSQTVNRLLARRLLLDR
IERLKTGRIAAEKARIEKIRRQKRRRSQRAQEKVLELKHRQSERKKLREKVGTEDA

Sequences:

>Translated_136_residues
MSISPEKEKDVAQRMARLGISEDDLRETFIRSSGPGGQNVNKTATCVYLVHLPTGLSVKCQQDRSQTVNRLLARRLLLDR
IERLKTGRIAAEKARIEKIRRQKRRRSQRAQEKVLELKHRQSERKKLREKVGTEDA
>Mature_135_residues
SISPEKEKDVAQRMARLGISEDDLRETFIRSSGPGGQNVNKTATCVYLVHLPTGLSVKCQQDRSQTVNRLLARRLLLDRI
ERLKTGRIAAEKARIEKIRRQKRRRSQRAQEKVLELKHRQSERKKLREKVGTEDA

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI303304971, Length=87, Percent_Identity=39.0804597701149, Blast_Score=77, Evalue=4e-15,
Organism=Homo sapiens, GI219879802, Length=87, Percent_Identity=39.0804597701149, Blast_Score=77, Evalue=4e-15,
Organism=Homo sapiens, GI22748627, Length=87, Percent_Identity=39.0804597701149, Blast_Score=77, Evalue=4e-15,
Organism=Homo sapiens, GI34577120, Length=58, Percent_Identity=55.1724137931034, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI2367172, Length=95, Percent_Identity=35.7894736842105, Blast_Score=62, Evalue=9e-12,
Organism=Escherichia coli, GI1787462, Length=86, Percent_Identity=41.8604651162791, Blast_Score=61, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24654113, Length=110, Percent_Identity=44.5454545454545, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19921226, Length=93, Percent_Identity=40.8602150537634, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 15744; Mature: 15613

Theoretical pI: Translated: 11.41; Mature: 11.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISPEKEKDVAQRMARLGISEDDLRETFIRSSGPGGQNVNKTATCVYLVHLPTGLSVKC
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHEEEEECCCCCCEEE
QQDRSQTVNRLLARRLLLDRIERLKTGRIAAEKARIEKIRRQKRRRSQRAQEKVLELKHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSERKKLREKVGTEDA
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SISPEKEKDVAQRMARLGISEDDLRETFIRSSGPGGQNVNKTATCVYLVHLPTGLSVKC
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHEEEEECCCCCCEEE
QQDRSQTVNRLLARRLLLDRIERLKTGRIAAEKARIEKIRRQKRRRSQRAQEKVLELKHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSERKKLREKVGTEDA
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA