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The map label for this gene is argB

Identifier: 85859271

GI number: 85859271

Start: 1458171

End: 1459061

Strand: Direct

Name: argB

Synonym: SYN_02154

Alternate gene names: 85859271

Gene position: 1458171-1459061 (Clockwise)

Preceding gene: 85859270

Following gene: 85859272

Centisome position: 45.86

GC content: 48.82

Gene sequence:

>891_bases
ATGAACACGATAGAAAAACCCACGGAAAAGGCGGAGATCCTGCTGGAAGCGCTTCCTTACATCCGTCGCTTTTACAACCG
AACCGTTGTCATCAAATACGGCGGACACGCCATGGTGGATGATGAACTGAAGCAATCTTTTGCCAAAGATGTCCTGATGA
TGAAATATATCGGAATCAATCCCGTGGTCGTTCATGGAGGAGGACCGCAGATCGGCTCTTTTCTTAAAAAACTGGGTAAA
GACTCCAAATTCATCCAGGGAATGCGGGTAACAGACGAAGAAACCATGAACATTGTCGAAATGGTTCTCGTAGGCAAAGT
CAACAAGGAAATCGTCGGTCTTATCAACCATGCTGGGGGCAAAGCCGTGGGCCTGAGCGGCAAGGACGGCAGTCTCATTC
GGGCGGAAAAGTATTACCTCAGTGCGGAGAAGGCAAAGGATACTCCACCGGAAATCATTGATATCGGACTTGTGGGCAAG
GTTAAGGAAATTAATGCGGATTTGATTACATCCCTTGTCCGCGACGGCTTCATTCCAGTCATTGCCCCAACCGGAGCCGG
AGACAGTGGGGAGACCTATAACATCAATGCCGATATTGTGGCAGGCGCCATAGCCGCCGCCCTCAAGGCGGAAAAACTGA
TTCTGCTGACAGATGTAGCGGGCGTATTGAATAAACAGGGCGAATTGATCAATACCATGAACAATATGGAGGCACTCGAA
ATGATTCATGAAGGTGTCATCGAAGGCGGCATGTTCCCCAAAGTAAAATGCTGCATGAAGTCATTGAGGGAAGGGGTACG
CAAGGCTCATATCGTTGACGGACGGCTGAAACATGCCATTCTGCTGGAAATGTTCACCGATAAGGGAATTGGAACGGAAC
TCGTACTGTAA

Upstream 100 bases:

>100_bases
AGCCGTTACATCCTGTAACTTGCCTTTTTCCTGGCTTTGCAGCATGGGGTGATGCCTGAGATTTATTATTTGAAGATATA
TTAATGAGGATGGAGTCATA

Downstream 100 bases:

>100_bases
TTACTTGCGGAAGGCGGTTCAAAGGACGGATTTCAAAAACTGGATAATGGAAACTCGCCCTTGGGAGGGATAGGGAATGA
CAACAGAGGAACTGATTGCG

Product: acetylglutamate kinase

Products: NA

Alternate protein names: N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AGK

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNTIEKPTEKAEILLEALPYIRRFYNRTVVIKYGGHAMVDDELKQSFAKDVLMMKYIGINPVVVHGGGPQIGSFLKKLGK
DSKFIQGMRVTDEETMNIVEMVLVGKVNKEIVGLINHAGGKAVGLSGKDGSLIRAEKYYLSAEKAKDTPPEIIDIGLVGK
VKEINADLITSLVRDGFIPVIAPTGAGDSGETYNINADIVAGAIAAALKAEKLILLTDVAGVLNKQGELINTMNNMEALE
MIHEGVIEGGMFPKVKCCMKSLREGVRKAHIVDGRLKHAILLEMFTDKGIGTELVL

Sequences:

>Translated_296_residues
MNTIEKPTEKAEILLEALPYIRRFYNRTVVIKYGGHAMVDDELKQSFAKDVLMMKYIGINPVVVHGGGPQIGSFLKKLGK
DSKFIQGMRVTDEETMNIVEMVLVGKVNKEIVGLINHAGGKAVGLSGKDGSLIRAEKYYLSAEKAKDTPPEIIDIGLVGK
VKEINADLITSLVRDGFIPVIAPTGAGDSGETYNINADIVAGAIAAALKAEKLILLTDVAGVLNKQGELINTMNNMEALE
MIHEGVIEGGMFPKVKCCMKSLREGVRKAHIVDGRLKHAILLEMFTDKGIGTELVL
>Mature_296_residues
MNTIEKPTEKAEILLEALPYIRRFYNRTVVIKYGGHAMVDDELKQSFAKDVLMMKYIGINPVVVHGGGPQIGSFLKKLGK
DSKFIQGMRVTDEETMNIVEMVLVGKVNKEIVGLINHAGGKAVGLSGKDGSLIRAEKYYLSAEKAKDTPPEIIDIGLVGK
VKEINADLITSLVRDGFIPVIAPTGAGDSGETYNINADIVAGAIAAALKAEKLILLTDVAGVLNKQGELINTMNNMEALE
MIHEGVIEGGMFPKVKCCMKSLREGVRKAHIVDGRLKHAILLEMFTDKGIGTELVL

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetylglutamate kinase family

Homologues:

Organism=Escherichia coli, GI1789181, Length=300, Percent_Identity=26.6666666666667, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI145693208, Length=269, Percent_Identity=31.2267657992565, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320913, Length=265, Percent_Identity=27.9245283018868, Blast_Score=97, Evalue=4e-21,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGB_SYNAS (Q2LT95)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461473.1
- ProteinModelPortal:   Q2LT95
- SMR:   Q2LT95
- STRING:   Q2LT95
- GeneID:   3884304
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02154
- NMPDR:   fig|56780.10.peg.1411
- eggNOG:   COG0548
- HOGENOM:   HBG497643
- OMA:   MDIVEMV
- PhylomeDB:   Q2LT95
- BioCyc:   SACI56780:SYN_02154-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00082_B
- InterPro:   IPR004662
- InterPro:   IPR001048
- InterPro:   IPR001057
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000728
- PRINTS:   PR00474
- TIGRFAMs:   TIGR00761

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.8

Molecular weight: Translated: 32072; Mature: 32072

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: BINDING 89-89 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIEKPTEKAEILLEALPYIRRFYNRTVVIKYGGHAMVDDELKQSFAKDVLMMKYIGIN
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECHHHHHHHHHHHHHHHHCCCC
PVVVHGGGPQIGSFLKKLGKDSKFIQGMRVTDEETMNIVEMVLVGKVNKEIVGLINHAGG
EEEEECCCCHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCC
KAVGLSGKDGSLIRAEKYYLSAEKAKDTPPEIIDIGLVGKVKEINADLITSLVRDGFIPV
CEEECCCCCCCEEEHHHHEEEHHHCCCCCCCEEEECCCCCHHHCCHHHHHHHHHCCCEEE
IAPTGAGDSGETYNINADIVAGAIAAALKAEKLILLTDVAGVLNKQGELINTMNNMEALE
EEECCCCCCCCEEECCHHHHHHHHHHHHHHCCEEEEEHHHHHHCCCCHHHHHHHHHHHHH
MIHEGVIEGGMFPKVKCCMKSLREGVRKAHIVDGRLKHAILLEMFTDKGIGTELVL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNTIEKPTEKAEILLEALPYIRRFYNRTVVIKYGGHAMVDDELKQSFAKDVLMMKYIGIN
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEECHHHHHHHHHHHHHHHHCCCC
PVVVHGGGPQIGSFLKKLGKDSKFIQGMRVTDEETMNIVEMVLVGKVNKEIVGLINHAGG
EEEEECCCCHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCC
KAVGLSGKDGSLIRAEKYYLSAEKAKDTPPEIIDIGLVGKVKEINADLITSLVRDGFIPV
CEEECCCCCCCEEEHHHHEEEHHHCCCCCCCEEEECCCCCHHHCCHHHHHHHHHCCCEEE
IAPTGAGDSGETYNINADIVAGAIAAALKAEKLILLTDVAGVLNKQGELINTMNNMEALE
EEECCCCCCCCEEECCHHHHHHHHHHHHHHCCEEEEEHHHHHHCCCCHHHHHHHHHHHHH
MIHEGVIEGGMFPKVKCCMKSLREGVRKAHIVDGRLKHAILLEMFTDKGIGTELVL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA