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The map label for this gene is hslU

Identifier: 85859270

GI number: 85859270

Start: 1456706

End: 1458088

Strand: Direct

Name: hslU

Synonym: SYN_02153

Alternate gene names: 85859270

Gene position: 1456706-1458088 (Clockwise)

Preceding gene: 85859269

Following gene: 85859271

Centisome position: 45.82

GC content: 49.6

Gene sequence:

>1383_bases
ATGTCATCAAATTCATTAACACCTGCGGAAATCGTCGAAAAGCTTGATCAGCATATTATTGGGCAGGCCGATGCCAAGCG
TGCGGTTGCCATTGCCCTGAGAAACCGCTGGCGGCGTCAGAATGTGCCCGATGAACTCAGGGACGAAATCGCTCCGAAGA
ACATCATCATGATCGGACCGACAGGCGTGGGAAAGACGGAGATAGCTCGAAGGCTGGCCAAGATCGATAATTCTCCTTTC
CTCAAGGTGGAAGCCTCGAAGTTCACCGAAGTGGGATATGTGGGGCGGGATGTGGAATCCATGATCCGTGATCTGGTGGA
TCTGGCCGTGAACATGGTTAGAGAGGAGGAGCAGGAAAAGGTCCTTGCTCGGGCAGCAGAGATTGCCGAGGAAAGGCTTC
TGGATCTTCTGCTGCCTCCAAAGCCCGTTGAGCGGAATGTTGATGAGATGATGGCCGATCATTCCCAGTTGCCGGAAAAA
AGCGAACAGGCCAAGCCCTACGATTCAACAAGAGAAAAACTGCGCCGTCTCCTGCATGACGGAAAGCTGAATGGCCGCTA
TGTGGATCTGGAATTGACCGAGACACGCAGCGCACCAATGATTGAAATCTTTTCTTCATCGGGCGTTGAAGATCTGGGGC
TGAACCTTAAGGAAATGTTTGGGAACATGCTTCCGCAGAAGAAAAAAATCAGCCGCGTCAATGTTACAGAGGCATTGGAA
ATTCTGACGGAAGAAGAGGCCCAGCGTCTTGTGGATATGGATAAGGTTACCCGGACGGCACTCGATCGGGTGGAGCAATC
AGGAATTATTTTTCTCGATGAAATTGATAAAGTTGTGGGGAGTGACACTCCTTCAGGACCGGATGTATCCCGGGAAGGGG
TCCAGCGCGATCTTTTGCCCATTGTCGAAGGGTCAAATGTTAATACCCGGTATGGCATGGTCCGAACCGACCATATCCTT
TTTATAGCCGCCGGCGCCTTCAGTTCTTCCAAACCATCGGATCTCATCCCCGAACTCCAGGGAAGATTTCCAATCCGTGT
TGAATTGGATTCTCTGGGGAAGGAGGAGTTTATCCGGATTCTCAAAGAACCCAACAACGCCCTGATCAAACAGTACACGG
AAATGATGGCAACCGAAGGGGTTTCGCTCTTTTTTACAGATGATTCCATTGCTGAAATTGCCGACGTCGCCGCTGTTGTC
AATGAAAGAACAGAAAATATCGGTGCGAGGCGGCTGTATACGATCATGGAGATTCTCCTGGAGGATATTTCCTTCAATGC
CCCAGGGTTATCAGGGCAAAAAGTTGTTATCGATGAGCAATATGTTGAAGACAAACTTTGCGATATAATTGAAGACGAAG
ATCTCAGCCGTTACATCCTGTAA

Upstream 100 bases:

>100_bases
GGTAGAAGAACTGGATACCGAACAGGAAAGCGAACCGGTAGAGAAAAAAACACGGAGAATAAAGCCCCGGGGGTAGATAG
TCGCCGGAAGGAGGCTGTTC

Downstream 100 bases:

>100_bases
CTTGCCTTTTTCCTGGCTTTGCAGCATGGGGTGATGCCTGAGATTTATTATTTGAAGATATATTAATGAGGATGGAGTCA
TAATGAACACGATAGAAAAA

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSSNSLTPAEIVEKLDQHIIGQADAKRAVAIALRNRWRRQNVPDELRDEIAPKNIIMIGPTGVGKTEIARRLAKIDNSPF
LKVEASKFTEVGYVGRDVESMIRDLVDLAVNMVREEEQEKVLARAAEIAEERLLDLLLPPKPVERNVDEMMADHSQLPEK
SEQAKPYDSTREKLRRLLHDGKLNGRYVDLELTETRSAPMIEIFSSSGVEDLGLNLKEMFGNMLPQKKKISRVNVTEALE
ILTEEEAQRLVDMDKVTRTALDRVEQSGIIFLDEIDKVVGSDTPSGPDVSREGVQRDLLPIVEGSNVNTRYGMVRTDHIL
FIAAGAFSSSKPSDLIPELQGRFPIRVELDSLGKEEFIRILKEPNNALIKQYTEMMATEGVSLFFTDDSIAEIADVAAVV
NERTENIGARRLYTIMEILLEDISFNAPGLSGQKVVIDEQYVEDKLCDIIEDEDLSRYIL

Sequences:

>Translated_460_residues
MSSNSLTPAEIVEKLDQHIIGQADAKRAVAIALRNRWRRQNVPDELRDEIAPKNIIMIGPTGVGKTEIARRLAKIDNSPF
LKVEASKFTEVGYVGRDVESMIRDLVDLAVNMVREEEQEKVLARAAEIAEERLLDLLLPPKPVERNVDEMMADHSQLPEK
SEQAKPYDSTREKLRRLLHDGKLNGRYVDLELTETRSAPMIEIFSSSGVEDLGLNLKEMFGNMLPQKKKISRVNVTEALE
ILTEEEAQRLVDMDKVTRTALDRVEQSGIIFLDEIDKVVGSDTPSGPDVSREGVQRDLLPIVEGSNVNTRYGMVRTDHIL
FIAAGAFSSSKPSDLIPELQGRFPIRVELDSLGKEEFIRILKEPNNALIKQYTEMMATEGVSLFFTDDSIAEIADVAAVV
NERTENIGARRLYTIMEILLEDISFNAPGLSGQKVVIDEQYVEDKLCDIIEDEDLSRYIL
>Mature_459_residues
SSNSLTPAEIVEKLDQHIIGQADAKRAVAIALRNRWRRQNVPDELRDEIAPKNIIMIGPTGVGKTEIARRLAKIDNSPFL
KVEASKFTEVGYVGRDVESMIRDLVDLAVNMVREEEQEKVLARAAEIAEERLLDLLLPPKPVERNVDEMMADHSQLPEKS
EQAKPYDSTREKLRRLLHDGKLNGRYVDLELTETRSAPMIEIFSSSGVEDLGLNLKEMFGNMLPQKKKISRVNVTEALEI
LTEEEAQRLVDMDKVTRTALDRVEQSGIIFLDEIDKVVGSDTPSGPDVSREGVQRDLLPIVEGSNVNTRYGMVRTDHILF
IAAGAFSSSKPSDLIPELQGRFPIRVELDSLGKEEFIRILKEPNNALIKQYTEMMATEGVSLFFTDDSIAEIADVAAVVN
ERTENIGARRLYTIMEILLEDISFNAPGLSGQKVVIDEQYVEDKLCDIIEDEDLSRYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily [H]

Homologues:

Organism=Homo sapiens, GI7242140, Length=111, Percent_Identity=38.7387387387387, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790366, Length=458, Percent_Identity=54.585152838428, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1786642, Length=235, Percent_Identity=34.468085106383, Blast_Score=102, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71982908, Length=277, Percent_Identity=29.2418772563177, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71982905, Length=277, Percent_Identity=29.2418772563177, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71988663, Length=258, Percent_Identity=30.2325581395349, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319704, Length=123, Percent_Identity=38.2113821138211, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648291, Length=246, Percent_Identity=32.1138211382114, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648289, Length=246, Percent_Identity=32.1138211382114, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 51718; Mature: 51587

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNSLTPAEIVEKLDQHIIGQADAKRAVAIALRNRWRRQNVPDELRDEIAPKNIIMIGP
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECC
TGVGKTEIARRLAKIDNSPFLKVEASKFTEVGYVGRDVESMIRDLVDLAVNMVREEEQEK
CCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VLARAAEIAEERLLDLLLPPKPVERNVDEMMADHSQLPEKSEQAKPYDSTREKLRRLLHD
HHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHC
GKLNGRYVDLELTETRSAPMIEIFSSSGVEDLGLNLKEMFGNMLPQKKKISRVNVTEALE
CCCCCEEEEEEEECCCCCCEEEECCCCCCHHHCCCHHHHHHHHCCCHHHHHHCCHHHHHH
ILTEEEAQRLVDMDKVTRTALDRVEQSGIIFLDEIDKVVGSDTPSGPDVSREGVQRDLLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
IVEGSNVNTRYGMVRTDHILFIAAGAFSSSKPSDLIPELQGRFPIRVELDSLGKEEFIRI
HCCCCCCCCEECEEEECEEEEEEECCCCCCCCHHHHHHHCCCCCEEEEHHHCCHHHHHHH
LKEPNNALIKQYTEMMATEGVSLFFTDDSIAEIADVAAVVNERTENIGARRLYTIMEILL
HHCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDISFNAPGLSGQKVVIDEQYVEDKLCDIIEDEDLSRYIL
HHHCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SSNSLTPAEIVEKLDQHIIGQADAKRAVAIALRNRWRRQNVPDELRDEIAPKNIIMIGP
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECC
TGVGKTEIARRLAKIDNSPFLKVEASKFTEVGYVGRDVESMIRDLVDLAVNMVREEEQEK
CCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VLARAAEIAEERLLDLLLPPKPVERNVDEMMADHSQLPEKSEQAKPYDSTREKLRRLLHD
HHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHCCCCHHCCCCCHHHHHHHHHHHHC
GKLNGRYVDLELTETRSAPMIEIFSSSGVEDLGLNLKEMFGNMLPQKKKISRVNVTEALE
CCCCCEEEEEEEECCCCCCEEEECCCCCCHHHCCCHHHHHHHHCCCHHHHHHCCHHHHHH
ILTEEEAQRLVDMDKVTRTALDRVEQSGIIFLDEIDKVVGSDTPSGPDVSREGVQRDLLP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
IVEGSNVNTRYGMVRTDHILFIAAGAFSSSKPSDLIPELQGRFPIRVELDSLGKEEFIRI
HCCCCCCCCEECEEEECEEEEEEECCCCCCCCHHHHHHHCCCCCEEEEHHHCCHHHHHHH
LKEPNNALIKQYTEMMATEGVSLFFTDDSIAEIADVAAVVNERTENIGARRLYTIMEILL
HHCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDISFNAPGLSGQKVVIDEQYVEDKLCDIIEDEDLSRYIL
HHHCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA