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The map label for this gene is bacA [H]

Identifier: 85858227

GI number: 85858227

Start: 380643

End: 382241

Strand: Direct

Name: bacA [H]

Synonym: SYN_01145

Alternate gene names: 85858227

Gene position: 380643-382241 (Clockwise)

Preceding gene: 85858226

Following gene: 85858228

Centisome position: 11.97

GC content: 52.97

Gene sequence:

>1599_bases
ATGCCGAACCACCTACTGCACCATCAACTGACCAATACGTCCTGGCGATGCCCGGAAAAAGAAGCGGTCATCGCCGTTGA
TCGGTCGATATCCTACCAGGAACTGGAGGAGCGGAGCAATCAGCTTGCCTCGGTCCTCATGCGCGCCGGTGTTCAAAAGG
GAGACCGGGTCGGGATATTTCTTCCCAAGTCCATCGAATCCCTGATCAGCCTGTTCGCTATTCTGAAAAACGGCTCCACT
TATGTTCCCATCGATCCCAGGCTGCCTTCCGGCAGAATAGAGTACATTCTTCAAAATTGCGACATCGCGTGCATGATTTC
CTCCTCCGCAACGTTGAAGAAAGTCCTGGCGGATTTGAGCGATGACCTTGCCCTGAAAAAGGTCATTCTTACCGACTGGG
ATGGGGATGAAAACGAAAAGCCGGAGGGACATCCGCAATTCATCCCCTGGACGAGCATCAGGGATGAGGATGGACAGAGG
AATCCGGGACCGGACATCGCCGATGTCAATCCCGCCTACATCCTCTATACATCCGGATCGACGGGCACCCCCAAGGGGGT
GGCCATCTCCCACTTGAATTCCCTCACGTTCGTCAATATGGCCGCTGAATATTTCGGCATCGATACTCCGGACAGGCTGG
CGAGTTTCGCCCCCCTTCATTTCGATCTGTCGATCTTCGACATTTTTACCGCGGTCCGTCAAGGGGCCTCCATTGTCCTC
GTCCCTGAATTTTATTCCACGTTTCCGATAAGACTCGCGGAATATATCGATAAACAGCGCGTCACCGTCTGGAATTCCGT
TTCTTCCGTCCTCATCATGCTCGTGGATATGGGAAAGCTCGAGAAGTGCGGCTTTGAAACGCTCAGGCTCATTCATTTTT
CCGGTGACATTCTTCCTCCCAGATACCTCCGGGAATTGAAAAGTCATATGAAAAACGCGGACTTCTACAATATCTACGGC
CAGACCGAGGCGAACTCGTCCATGTGTTATCACATCGGCGCCGTTCCGCCGGGTGATCACTGGAAAATCCCCATCGGCAA
ACCGTTTCCCAATTTTGAAGTTTTCGCGGTCAACGAGGATGGGCGGAAAATATCCTCGCCCGGCCAGGAGGGTGAACTTT
ACGTCAAAAGCTCCACGGTTGCCGTCGGGTACTGGAATGACGGAGAAAGAACGAAGGAGCGATTCACTTCCAATCCCCTG
GATCCGGCTGACGCGTCACGATGCTATAAAACAGGCGATATTGTAAAACTGGATGACAAAGGAAATTTTGTCTTCGTGGG
GCGCAAAGACTCCATGGTGAAGAGCAGGGGGTACCGCATCGAGCTCAATGAAATCGAAATCATTCTGAACGGCCATCCCC
TGGTGAGACAGGCCGTGGCCGTCGCGGCGCCCGATGAAAAAATAGGCCACCTAGTGGTCGGTTTCGCGTCGCTCGTTGAA
GGAGAATCCCTGTCGGAAATCGATCTCCTTCGTTACTGTCAGGATCGTCTGCCCAAATACATGGTTCCGGAATCGATTCA
CTTTTTGAAGCGCCTTCCGACGACGTCGTCGGGAAAAGTGGACCGGAAAGCGATGGAAAAAGAGGCGCTGGAAGGGTAG

Upstream 100 bases:

>100_bases
TCTCCGTGATTCCATCGCCGGAAAAATCTATTCCGGAAGCTCCGAGATTCAGAGAAATATCCTGGCAAGCTTTCTGGGGT
TATAGCGGAGGGGATAAAGA

Downstream 100 bases:

>100_bases
GAGGAATTCAACCCGTGCGTATTCTTGAAATCGGCATGTTCTATGAACCGGATCTCGGCCCCGGCGCTCCCCTGTGCACG
ATGCTCAGCAGAGAGCTGGC

Product: fatty acid-CoA ligase

Products: NA

Alternate protein names: BA1; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; ATP-dependent cysteine adenylase; CysA; Cysteine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase; ATP-dependent glutamate adenylase; GluA; Glutamate activase; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; Glutamate racemase [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MPNHLLHHQLTNTSWRCPEKEAVIAVDRSISYQELEERSNQLASVLMRAGVQKGDRVGIFLPKSIESLISLFAILKNGST
YVPIDPRLPSGRIEYILQNCDIACMISSSATLKKVLADLSDDLALKKVILTDWDGDENEKPEGHPQFIPWTSIRDEDGQR
NPGPDIADVNPAYILYTSGSTGTPKGVAISHLNSLTFVNMAAEYFGIDTPDRLASFAPLHFDLSIFDIFTAVRQGASIVL
VPEFYSTFPIRLAEYIDKQRVTVWNSVSSVLIMLVDMGKLEKCGFETLRLIHFSGDILPPRYLRELKSHMKNADFYNIYG
QTEANSSMCYHIGAVPPGDHWKIPIGKPFPNFEVFAVNEDGRKISSPGQEGELYVKSSTVAVGYWNDGERTKERFTSNPL
DPADASRCYKTGDIVKLDDKGNFVFVGRKDSMVKSRGYRIELNEIEIILNGHPLVRQAVAVAAPDEKIGHLVVGFASLVE
GESLSEIDLLRYCQDRLPKYMVPESIHFLKRLPTTSSGKVDRKAMEKEALEG

Sequences:

>Translated_532_residues
MPNHLLHHQLTNTSWRCPEKEAVIAVDRSISYQELEERSNQLASVLMRAGVQKGDRVGIFLPKSIESLISLFAILKNGST
YVPIDPRLPSGRIEYILQNCDIACMISSSATLKKVLADLSDDLALKKVILTDWDGDENEKPEGHPQFIPWTSIRDEDGQR
NPGPDIADVNPAYILYTSGSTGTPKGVAISHLNSLTFVNMAAEYFGIDTPDRLASFAPLHFDLSIFDIFTAVRQGASIVL
VPEFYSTFPIRLAEYIDKQRVTVWNSVSSVLIMLVDMGKLEKCGFETLRLIHFSGDILPPRYLRELKSHMKNADFYNIYG
QTEANSSMCYHIGAVPPGDHWKIPIGKPFPNFEVFAVNEDGRKISSPGQEGELYVKSSTVAVGYWNDGERTKERFTSNPL
DPADASRCYKTGDIVKLDDKGNFVFVGRKDSMVKSRGYRIELNEIEIILNGHPLVRQAVAVAAPDEKIGHLVVGFASLVE
GESLSEIDLLRYCQDRLPKYMVPESIHFLKRLPTTSSGKVDRKAMEKEALEG
>Mature_531_residues
PNHLLHHQLTNTSWRCPEKEAVIAVDRSISYQELEERSNQLASVLMRAGVQKGDRVGIFLPKSIESLISLFAILKNGSTY
VPIDPRLPSGRIEYILQNCDIACMISSSATLKKVLADLSDDLALKKVILTDWDGDENEKPEGHPQFIPWTSIRDEDGQRN
PGPDIADVNPAYILYTSGSTGTPKGVAISHLNSLTFVNMAAEYFGIDTPDRLASFAPLHFDLSIFDIFTAVRQGASIVLV
PEFYSTFPIRLAEYIDKQRVTVWNSVSSVLIMLVDMGKLEKCGFETLRLIHFSGDILPPRYLRELKSHMKNADFYNIYGQ
TEANSSMCYHIGAVPPGDHWKIPIGKPFPNFEVFAVNEDGRKISSPGQEGELYVKSSTVAVGYWNDGERTKERFTSNPLD
PADASRCYKTGDIVKLDDKGNFVFVGRKDSMVKSRGYRIELNEIEIILNGHPLVRQAVAVAAPDEKIGHLVVGFASLVEG
ESLSEIDLLRYCQDRLPKYMVPESIHFLKRLPTTSSGKVDRKAMEKEALEG

Specific function: Activates five amino acids, incorporates two D-amino acids, releases and cyclizes the mature bacitracin [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=551, Percent_Identity=23.7749546279492, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI187761345, Length=540, Percent_Identity=26.1111111111111, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI187761343, Length=540, Percent_Identity=26.1111111111111, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI38505220, Length=510, Percent_Identity=26.6666666666667, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI42544132, Length=521, Percent_Identity=27.6391554702495, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI122937307, Length=514, Percent_Identity=25.2918287937743, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI157311624, Length=513, Percent_Identity=24.1715399610136, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI157311622, Length=513, Percent_Identity=24.1715399610136, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=23.6994219653179, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI58082049, Length=513, Percent_Identity=24.3664717348928, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI45580730, Length=529, Percent_Identity=23.6294896030246, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI8923896, Length=553, Percent_Identity=24.2314647377938, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI253970489, Length=566, Percent_Identity=24.0282685512367, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI195947378, Length=423, Percent_Identity=23.8770685579196, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1786801, Length=538, Percent_Identity=27.3234200743494, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788107, Length=546, Percent_Identity=26.007326007326, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI145693145, Length=524, Percent_Identity=24.618320610687, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=27.2904483430799, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1790505, Length=528, Percent_Identity=23.6742424242424, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786810, Length=530, Percent_Identity=23.9622641509434, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786529, Length=570, Percent_Identity=22.6315789473684, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994690, Length=550, Percent_Identity=25.6363636363636, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=550, Percent_Identity=25.6363636363636, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=548, Percent_Identity=25.7299270072993, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17560308, Length=570, Percent_Identity=24.3859649122807, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=26.0355029585799, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=534, Percent_Identity=21.9101123595506, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=536, Percent_Identity=22.2014925373134, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983001, Length=538, Percent_Identity=24.907063197026, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71982997, Length=538, Percent_Identity=24.907063197026, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=524, Percent_Identity=22.5190839694656, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=516, Percent_Identity=22.6744186046512, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI71996755, Length=539, Percent_Identity=23.191094619666, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32564420, Length=357, Percent_Identity=26.0504201680672, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564422, Length=357, Percent_Identity=26.0504201680672, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=367, Percent_Identity=23.9782016348774, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=382, Percent_Identity=21.2041884816754, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534440, Length=565, Percent_Identity=22.6548672566372, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556356, Length=359, Percent_Identity=25.6267409470752, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=543, Percent_Identity=21.5469613259669, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319591, Length=593, Percent_Identity=25.4637436762226, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319699, Length=541, Percent_Identity=21.81146025878, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323182, Length=579, Percent_Identity=26.9430051813472, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319264, Length=555, Percent_Identity=25.2252252252252, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648676, Length=528, Percent_Identity=27.0833333333333, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24581924, Length=551, Percent_Identity=24.8638838475499, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI62472339, Length=539, Percent_Identity=25.7884972170686, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24667955, Length=539, Percent_Identity=25.7884972170686, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355181, Length=524, Percent_Identity=22.1374045801527, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18859661, Length=542, Percent_Identity=22.6937269372694, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648260, Length=504, Percent_Identity=23.6111111111111, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24667959, Length=413, Percent_Identity=27.1186440677966, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656500, Length=510, Percent_Identity=22.7450980392157, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653035, Length=512, Percent_Identity=20.5078125, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648257, Length=522, Percent_Identity=23.3716475095785, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358303, Length=487, Percent_Identity=20.5338809034908, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI161076582, Length=518, Percent_Identity=20.2702702702703, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356947, Length=357, Percent_Identity=23.5294117647059, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648253, Length=521, Percent_Identity=21.4971209213052, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24648255, Length=521, Percent_Identity=21.3051823416507, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21356441, Length=528, Percent_Identity=21.0227272727273, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR013624
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF08415 NRPS; PF00550 PP-binding [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 59403; Mature: 59272

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNHLLHHQLTNTSWRCPEKEAVIAVDRSISYQELEERSNQLASVLMRAGVQKGDRVGIF
CCCCCHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LPKSIESLISLFAILKNGSTYVPIDPRLPSGRIEYILQNCDIACMISSSATLKKVLADLS
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCC
DDLALKKVILTDWDGDENEKPEGHPQFIPWTSIRDEDGQRNPGPDIADVNPAYILYTSGS
CCHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
TGTPKGVAISHLNSLTFVNMAAEYFGIDTPDRLASFAPLHFDLSIFDIFTAVRQGASIVL
CCCCCCEEHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCEEECHHHHHHHHHHHCCCEEEE
VPEFYSTFPIRLAEYIDKQRVTVWNSVSSVLIMLVDMGKLEKCGFETLRLIHFSGDILPP
ECHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHCCHHHEEEEEECCCCCCH
RYLRELKSHMKNADFYNIYGQTEANSSMCYHIGAVPPGDHWKIPIGKPFPNFEVFAVNED
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCEEECCCCCCCCEEEEEECCC
GRKISSPGQEGELYVKSSTVAVGYWNDGERTKERFTSNPLDPADASRCYKTGDIVKLDDK
CCCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHCCCCCEEEECCC
GNFVFVGRKDSMVKSRGYRIELNEIEIILNGHPLVRQAVAVAAPDEKIGHLVVGFASLVE
CCEEEEECCCCHHHCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GESLSEIDLLRYCQDRLPKYMVPESIHFLKRLPTTSSGKVDRKAMEKEALEG
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PNHLLHHQLTNTSWRCPEKEAVIAVDRSISYQELEERSNQLASVLMRAGVQKGDRVGIF
CCCCHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LPKSIESLISLFAILKNGSTYVPIDPRLPSGRIEYILQNCDIACMISSSATLKKVLADLS
CCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCC
DDLALKKVILTDWDGDENEKPEGHPQFIPWTSIRDEDGQRNPGPDIADVNPAYILYTSGS
CCHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCC
TGTPKGVAISHLNSLTFVNMAAEYFGIDTPDRLASFAPLHFDLSIFDIFTAVRQGASIVL
CCCCCCEEHHHHCCHHHHHHHHHHHCCCCHHHHHHCCCEEECHHHHHHHHHHHCCCEEEE
VPEFYSTFPIRLAEYIDKQRVTVWNSVSSVLIMLVDMGKLEKCGFETLRLIHFSGDILPP
ECHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCHHHCCHHHEEEEEECCCCCCH
RYLRELKSHMKNADFYNIYGQTEANSSMCYHIGAVPPGDHWKIPIGKPFPNFEVFAVNED
HHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCCCEEECCCCCCCCEEEEEECCC
GRKISSPGQEGELYVKSSTVAVGYWNDGERTKERFTSNPLDPADASRCYKTGDIVKLDDK
CCCCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHCCCCCEEEECCC
GNFVFVGRKDSMVKSRGYRIELNEIEIILNGHPLVRQAVAVAAPDEKIGHLVVGFASLVE
CCEEEEECCCCHHHCCCCEEEEEEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHC
GESLSEIDLLRYCQDRLPKYMVPESIHFLKRLPTTSSGKVDRKAMEKEALEG
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9427658 [H]