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The map label for this gene is wcaI [H]

Identifier: 85858228

GI number: 85858228

Start: 382256

End: 383590

Strand: Direct

Name: wcaI [H]

Synonym: SYN_01144

Alternate gene names: 85858228

Gene position: 382256-383590 (Clockwise)

Preceding gene: 85858227

Following gene: 85858229

Centisome position: 12.02

GC content: 57.45

Gene sequence:

>1335_bases
GTGCGTATTCTTGAAATCGGCATGTTCTATGAACCGGATCTCGGCCCCGGCGCTCCCCTGTGCACGATGCTCAGCAGAGA
GCTGGCCAGTCTGGGGCATCAGGTCACGGCTCTCGCCGCGGTGCCGCATTATCCAACCGGACAGGTTCAGAGAGCGTTCC
GCGGCAGATGGCTCCGGACGACGGTTGAAGACGGGGTCGAGGTGGTGCGGGTTGGGCTTCCTTCGGTAAACCGGGCCGAT
CTCGTTCAGCGTTTTACCCAGTTTTTCTGCTATCAGGTGGGAGCCGCCCTGTCGGGACTGACCAAGAGATATGACGTTGT
TCTCGCGGCGAATCCCTTTCTGATGGTGTGGCTTCCCTTTGCGCTGCTGGGAGCCCTGAAGCATAAACCGATCGTCTATA
TCGTACAGGATTTATATCCTGATGTCGGCATCAAACTGGGGGTCTTCAAAAACCGGTTCGTAATCAGCGCCGCAACCGCC
CTGGAACGCTATTGCCTGGTGAATTCCGATGTCGTTCACATCATTTCCGATTCATTCCGGCCCAGCCTGCGCGCGCTCGG
CGTATCAGACGACAAGATGGCCCTGGTGTACAACTGGGTCGATACCGATTTGGTCCGTCCCTTGCCCCGGAATAATGCAT
TCGCTCAGGAACATGATTTAGGGGGTCGATTCGTAATTCTCTATGCGGGAAATATCGGGTTTTCCCAGGATCTGGACAAG
GTCCTGGATGCCGCCCAGGCTCTCGCGGATCAGGATGAAATCCTGTTCCTGTTCATCGGCGACGGGGTGGGGCGGGAACC
TCTGATCGCGGACGCGAAAAAGAGGCGGCTGACGAATGTGAAGTTTCTGCACTACCAGCCGCGGGAACGGCTGCCGGAAG
TGCTGGCTTGCGCGGATGTTTCACTGGTCATTCTGCGCGAGGGAATCGGTACGGCCTCTGTACCATCGAAGGCTCTTTCC
ATCCTGGCAAGCGGCAGGCCGATGGTGGCCAGTGTGGACGAAGGAAGCGACCTCTGGAATCTCGTGAAGGAAGCCGAGGC
GGGGCTGTGCATCCCTCCCGGCAGTTCGAACGATCTTGTCCAGGCTATCCTGACCCTCAAGCAGGACAAGGACCTGCGGG
AACGTCTGGGGAACAACGGGCGGACCTGGGCAGAAAAGCATCATTCCCCCCGGGTCGCCGCGAGAAGGTTCGAAAGTTTA
TTGCGGCAAGCCATTGCATGCAAAACGCGTGATGGCATCTGCCCGGCGAAAGCGTCAACTGAGCGGTCATCTTTGATTTC
AGGCAGCCAAAACGAAATCGATGGTCTTAACCGAGGTGGAAAAGATGGAACCTGA

Upstream 100 bases:

>100_bases
CGATTCACTTTTTGAAGCGCCTTCCGACGACGTCGTCGGGAAAAGTGGACCGGAAAGCGATGGAAAAAGAGGCGCTGGAA
GGGTAGGAGGAATTCAACCC

Downstream 100 bases:

>100_bases
AGTGTTTTCTGAATTTCTGATGCGGCAGGGTCATCATGTGGTCAAGACGGAAAGCTGCTACTGGTATGATGCTCAACCGG
GTTTTTACTTCTATTTCCCG

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRILEIGMFYEPDLGPGAPLCTMLSRELASLGHQVTALAAVPHYPTGQVQRAFRGRWLRTTVEDGVEVVRVGLPSVNRAD
LVQRFTQFFCYQVGAALSGLTKRYDVVLAANPFLMVWLPFALLGALKHKPIVYIVQDLYPDVGIKLGVFKNRFVISAATA
LERYCLVNSDVVHIISDSFRPSLRALGVSDDKMALVYNWVDTDLVRPLPRNNAFAQEHDLGGRFVILYAGNIGFSQDLDK
VLDAAQALADQDEILFLFIGDGVGREPLIADAKKRRLTNVKFLHYQPRERLPEVLACADVSLVILREGIGTASVPSKALS
ILASGRPMVASVDEGSDLWNLVKEAEAGLCIPPGSSNDLVQAILTLKQDKDLRERLGNNGRTWAEKHHSPRVAARRFESL
LRQAIACKTRDGICPAKASTERSSLISGSQNEIDGLNRGGKDGT

Sequences:

>Translated_444_residues
MRILEIGMFYEPDLGPGAPLCTMLSRELASLGHQVTALAAVPHYPTGQVQRAFRGRWLRTTVEDGVEVVRVGLPSVNRAD
LVQRFTQFFCYQVGAALSGLTKRYDVVLAANPFLMVWLPFALLGALKHKPIVYIVQDLYPDVGIKLGVFKNRFVISAATA
LERYCLVNSDVVHIISDSFRPSLRALGVSDDKMALVYNWVDTDLVRPLPRNNAFAQEHDLGGRFVILYAGNIGFSQDLDK
VLDAAQALADQDEILFLFIGDGVGREPLIADAKKRRLTNVKFLHYQPRERLPEVLACADVSLVILREGIGTASVPSKALS
ILASGRPMVASVDEGSDLWNLVKEAEAGLCIPPGSSNDLVQAILTLKQDKDLRERLGNNGRTWAEKHHSPRVAARRFESL
LRQAIACKTRDGICPAKASTERSSLISGSQNEIDGLNRGGKDGT
>Mature_444_residues
MRILEIGMFYEPDLGPGAPLCTMLSRELASLGHQVTALAAVPHYPTGQVQRAFRGRWLRTTVEDGVEVVRVGLPSVNRAD
LVQRFTQFFCYQVGAALSGLTKRYDVVLAANPFLMVWLPFALLGALKHKPIVYIVQDLYPDVGIKLGVFKNRFVISAATA
LERYCLVNSDVVHIISDSFRPSLRALGVSDDKMALVYNWVDTDLVRPLPRNNAFAQEHDLGGRFVILYAGNIGFSQDLDK
VLDAAQALADQDEILFLFIGDGVGREPLIADAKKRRLTNVKFLHYQPRERLPEVLACADVSLVILREGIGTASVPSKALS
ILASGRPMVASVDEGSDLWNLVKEAEAGLCIPPGSSNDLVQAILTLKQDKDLRERLGNNGRTWAEKHHSPRVAARRFESL
LRQAIACKTRDGICPAKASTERSSLISGSQNEIDGLNRGGKDGT

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=388, Percent_Identity=28.6082474226804, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 48727; Mature: 48727

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILEIGMFYEPDLGPGAPLCTMLSRELASLGHQVTALAAVPHYPTGQVQRAFRGRWLRT
CEEEEECCEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCHHHH
TVEDGVEVVRVGLPSVNRADLVQRFTQFFCYQVGAALSGLTKRYDVVLAANPFLMVWLPF
HHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
ALLGALKHKPIVYIVQDLYPDVGIKLGVFKNRFVISAATALERYCLVNSDVVHIISDSFR
HHHHHHCCCCEEEEHHHHCCCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCCEEHHCCCC
PSLRALGVSDDKMALVYNWVDTDLVRPLPRNNAFAQEHDLGGRFVILYAGNIGFSQDLDK
CHHHHCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCHHCCCCCEEEEEEECCCCCHHHHHH
VLDAAQALADQDEILFLFIGDGVGREPLIADAKKRRLTNVKFLHYQPRERLPEVLACADV
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCC
SLVILREGIGTASVPSKALSILASGRPMVASVDEGSDLWNLVKEAEAGLCIPPGSSNDLV
EEEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCEEECCCCCHHHH
QAILTLKQDKDLRERLGNNGRTWAEKHHSPRVAARRFESLLRQAIACKTRDGICPAKAST
HHHHHHHCCHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ERSSLISGSQNEIDGLNRGGKDGT
HHHHHHCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MRILEIGMFYEPDLGPGAPLCTMLSRELASLGHQVTALAAVPHYPTGQVQRAFRGRWLRT
CEEEEECCEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCHHHH
TVEDGVEVVRVGLPSVNRADLVQRFTQFFCYQVGAALSGLTKRYDVVLAANPFLMVWLPF
HHHCCHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
ALLGALKHKPIVYIVQDLYPDVGIKLGVFKNRFVISAATALERYCLVNSDVVHIISDSFR
HHHHHHCCCCEEEEHHHHCCCCCEEEEEECCCEEEHHHHHHHHHHHCCCCCCEEHHCCCC
PSLRALGVSDDKMALVYNWVDTDLVRPLPRNNAFAQEHDLGGRFVILYAGNIGFSQDLDK
CHHHHCCCCCCCEEEEEEEHHHHHHCCCCCCCCCCHHCCCCCEEEEEEECCCCCHHHHHH
VLDAAQALADQDEILFLFIGDGVGREPLIADAKKRRLTNVKFLHYQPRERLPEVLACADV
HHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCEEEEEECCHHHHHHHHHHCCC
SLVILREGIGTASVPSKALSILASGRPMVASVDEGSDLWNLVKEAEAGLCIPPGSSNDLV
EEEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCEEECCCCCHHHH
QAILTLKQDKDLRERLGNNGRTWAEKHHSPRVAARRFESLLRQAIACKTRDGICPAKAST
HHHHHHHCCHHHHHHCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
ERSSLISGSQNEIDGLNRGGKDGT
HHHHHHCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]