Click here to switch to the map view.

The map label for this gene is map [H]

Identifier: 85858168

GI number: 85858168

Start: 324088

End: 324837

Strand: Direct

Name: map [H]

Synonym: SYN_01596

Alternate gene names: 85858168

Gene position: 324088-324837 (Clockwise)

Preceding gene: 85858167

Following gene: 85858169

Centisome position: 10.19

GC content: 42.8

Gene sequence:

>750_bases
ATGGTTATTTTAAAGAGACCTGAAGAAATAGAAAAAATTCGAAAGAGCAGCCGGATCGTAGCGGAAATACTTCAGGAATT
AATGAATAGTGTAAAACCCGGAATAAGAACGATTGACCTTGAAGAGTACGCGGAAGAGCTGGCTGCCCGTAAAAAGGTAA
AACCCGCATTTAAAGGTTATAATGGCTTTCCATTTGGGCTTTGTGTGTCCGTTAATGAAGAAGTTGTGCATGGAATGCCT
TCAAAACGAATCTTACGTGATGGAGACATTGTCAGCTTGGATTACGGAGTTTATCATGACGGGTATTATGGAGATGCAGC
CATAACCGTGCCAGTCGGTGAGGTTTCACAGGAATTGTTAAGATTACTGCAGATTACAAAAGAGAGCTTATGCGAAGGGA
TTAAGGAATCAAGGATAGGGAACAGACTGGGTGATATCTCAGCCGCCATACAGGCTCATGTCGAAAGTGCGGGATTTTCA
GTGGTCAGGGATTTTGTGGGACATGGAATCGGTAAAAGTTTGCATGAAGATCCGCAGGTGCCGAATTTCGGATCAAAAGG
CAGAGGGATATTACTGAAAAAAGGAATGGTGCTTGCGATCGAGCCCATGGTAAATGCCGGCAAATATTCAGTTAAGGTAT
TAAATGATAATTGGACAGTCGTCACGGAAGATGGTAAGTATTCCGCTCACTTTGAGCACTCCGTTGCGATAACTGAAAAT
GGTCCGGATATCTTGAGTTGTATCAACTAA

Upstream 100 bases:

>100_bases
GGAAACACATCTTCTGACACGACAATATGAGGGATTTCTAAAGAAAGGCAAGATTGCAAGAAGAAGATGAAGAGACCTGC
CTTTTCCAGAAACAGATAAA

Downstream 100 bases:

>100_bases
GATCCGGAGATTACTGCGATGAAATACAGTTGAACGATCCAGAGGATCATAAGAAATTATTGCTAAAAGGATCTGCATTC
ATATTGAATCAAAGTAAGCA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP 1; Peptidase M [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MVILKRPEEIEKIRKSSRIVAEILQELMNSVKPGIRTIDLEEYAEELAARKKVKPAFKGYNGFPFGLCVSVNEEVVHGMP
SKRILRDGDIVSLDYGVYHDGYYGDAAITVPVGEVSQELLRLLQITKESLCEGIKESRIGNRLGDISAAIQAHVESAGFS
VVRDFVGHGIGKSLHEDPQVPNFGSKGRGILLKKGMVLAIEPMVNAGKYSVKVLNDNWTVVTEDGKYSAHFEHSVAITEN
GPDILSCIN

Sequences:

>Translated_249_residues
MVILKRPEEIEKIRKSSRIVAEILQELMNSVKPGIRTIDLEEYAEELAARKKVKPAFKGYNGFPFGLCVSVNEEVVHGMP
SKRILRDGDIVSLDYGVYHDGYYGDAAITVPVGEVSQELLRLLQITKESLCEGIKESRIGNRLGDISAAIQAHVESAGFS
VVRDFVGHGIGKSLHEDPQVPNFGSKGRGILLKKGMVLAIEPMVNAGKYSVKVLNDNWTVVTEDGKYSAHFEHSVAITEN
GPDILSCIN
>Mature_249_residues
MVILKRPEEIEKIRKSSRIVAEILQELMNSVKPGIRTIDLEEYAEELAARKKVKPAFKGYNGFPFGLCVSVNEEVVHGMP
SKRILRDGDIVSLDYGVYHDGYYGDAAITVPVGEVSQELLRLLQITKESLCEGIKESRIGNRLGDISAAIQAHVESAGFS
VVRDFVGHGIGKSLHEDPQVPNFGSKGRGILLKKGMVLAIEPMVNAGKYSVKVLNDNWTVVTEDGKYSAHFEHSVAITEN
GPDILSCIN

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=239, Percent_Identity=38.0753138075314, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI40385867, Length=243, Percent_Identity=33.3333333333333, Blast_Score=151, Evalue=4e-37,
Organism=Escherichia coli, GI1786364, Length=245, Percent_Identity=42.8571428571429, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1788728, Length=208, Percent_Identity=29.8076923076923, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789275, Length=220, Percent_Identity=25.9090909090909, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71996291, Length=240, Percent_Identity=36.6666666666667, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323273, Length=249, Percent_Identity=36.9477911646586, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319380, Length=266, Percent_Identity=27.0676691729323, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355531, Length=239, Percent_Identity=39.3305439330544, Blast_Score=185, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24583427, Length=243, Percent_Identity=33.7448559670782, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 27349; Mature: 27349

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVILKRPEEIEKIRKSSRIVAEILQELMNSVKPGIRTIDLEEYAEELAARKKVKPAFKGY
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCHHHCCC
NGFPFGLCVSVNEEVVHGMPSKRILRDGDIVSLDYGVYHDGYYGDAAITVPVGEVSQELL
CCCCEEEEEECCHHHHHCCCCCCEECCCCEEEEECCEEECCEECCEEEEEEHHHHHHHHH
RLLQITKESLCEGIKESRIGNRLGDISAAIQAHVESAGFSVVRDFVGHGIGKSLHEDPQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCC
PNFGSKGRGILLKKGMVLAIEPMVNAGKYSVKVLNDNWTVVTEDGKYSAHFEHSVAITEN
CCCCCCCCEEEEECCEEEEEECCCCCCCEEEEEECCCEEEEECCCCEEEEEEEEEEEECC
GPDILSCIN
CCHHHHHCC
>Mature Secondary Structure
MVILKRPEEIEKIRKSSRIVAEILQELMNSVKPGIRTIDLEEYAEELAARKKVKPAFKGY
CEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHCCHHHCCC
NGFPFGLCVSVNEEVVHGMPSKRILRDGDIVSLDYGVYHDGYYGDAAITVPVGEVSQELL
CCCCEEEEEECCHHHHHCCCCCCEECCCCEEEEECCEEECCEECCEEEEEEHHHHHHHHH
RLLQITKESLCEGIKESRIGNRLGDISAAIQAHVESAGFSVVRDFVGHGIGKSLHEDPQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCC
PNFGSKGRGILLKKGMVLAIEPMVNAGKYSVKVLNDNWTVVTEDGKYSAHFEHSVAITEN
CCCCCCCCEEEEECCEEEEEECCCCCCCEEEEEECCCEEEEECCCCEEEEEEEEEEEECC
GPDILSCIN
CCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113521; 8635744; 9384377 [H]