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The map label for this gene is hisS

Identifier: 85375679

GI number: 85375679

Start: 2863377

End: 2864726

Strand: Direct

Name: hisS

Synonym: ELI_14260

Alternate gene names: 85375679

Gene position: 2863377-2864726 (Clockwise)

Preceding gene: 85375677

Following gene: 85375680

Centisome position: 93.81

GC content: 59.48

Gene sequence:

>1350_bases
ATGAGCAAAAAAACTCCCCAGGCCATTCGCGGCACGCAGGACATCTTCAGTGCCGATGCCGAGGCTTTCGCTTTCGTGGT
CGAAACCTTCGAACGCGTGCGCAAGCTGTACCGCTTCCGCCGGGTCGAAATGCCGGTGTTCGAGAAAACCGAAGTCTTCA
GCCGCGCGATCGGCGAGACGACGGATGTGGTGTCGAAGGAGATGTACTCCTTCGAGGATCGCGGCGGCGACAGCTTGACC
CTGCGGCCCGAGTTCACCGCCGGGATTGCGCGCGCCTTCCTGACCAACGGCTGGCAACAACACGCGCCGCTGAAGGTCGC
GACCCACGGCCCGCTGTTTCGATACGAGCGTCCGCAAAAGGGCCGCTATCGGCAGTTCCACCAGATCGACGCCGAAATCA
TCGGCGCGGCCGAGCCGCAGGCGGACGTCGAACTCCTGGCGATGGCCGATCAGACCATCCGCGGACTGGGCATCGAGGGC
GTGACGTTGCACCTCAATACGCTCGGCGATGCCGAAAGCCGCGAAGCCTGGCGCGCAGCGCTGGTCGAGTACTTCCGCGC
CGTCGCCAGCGAGCTGAGTGAGGATTCGCAGGAGCGGCTGGAGAAGAACCCGCTGCGCATCCTCGACAGCAAGGATCGCC
GCGACCAGCAGTTCCTTGCCGATGCACCCAGGATCGATGCCTTCCTTTCCGATACAGCGCGGGCGTTCTTCGAAAGCGTG
ACCACCGGTTTGGATGCAGCAGGCGTCAAATGGCAGCGGGCAGAAAGTCTCGTGCGTGGGCTCGATTATTATCGCCACAC
CGCGTTCGAATTCATCCCCGATGAAGGTTCCGAGGCCGCGGGCAAGCTCGGCAGCCAGAGCACCATCCTCGGCGGCGGGC
GCTACGATGGATTGATGGAGTCGCTGGGCGGTGCGCCGACGCCTGCGGTTGGTTGGGCTGCCGGGATTGAGCGGCTGGCG
ATGTTGGTTGGGGGAGCAGGGTTTGACCCTCCTATTGTTGTACTGGCGGAGCATGAACACCTGTTCGACACGGCGAGGCT
CGTTGTGCAAGCATTGCGACTGAGTGAGATCACAGCGGAGGCCGAATTTCGTTATAGGCGGACGAACAAGGCGTTTGATA
AGTTCAAGAAGACTGGAACAGACGTCTTTTTAGTTGTTGACCAAGTGGCCAATTTAAATGGCAGTCATCAGATTGATATC
AGGCTATCTGGAGTGCTGCCGGATGAAGAGGAAGAATTAAAGCTCTTGGCCAAGAGAGTTGAATTAAGCCTCCGATTGTT
CTTTCCCGACCTAATACCGAAGGCTGATCCTGAGAAGCAAGCCATCTCTTGGTATCTCTCTCGCAAATGA

Upstream 100 bases:

>100_bases
CGCCGGATGGCGAAGGCGCACCCGACAGACATTGCGCGCGCCCCTTAACCTCCGCCGCGCCATTGCGAAACCCCTTCCAT
GGTCGTAATCGCGCCGCGAA

Downstream 100 bases:

>100_bases
CTATCCCCGCCACCCGCCTCGACCAGATCGCCAACCGCTTCGCCGAGCTTGAAGCGCGGATGGCATCGGGCACGCTGGAA
GGCGAGGAATTCGTGCAGGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSKKTPQAIRGTQDIFSADAEAFAFVVETFERVRKLYRFRRVEMPVFEKTEVFSRAIGETTDVVSKEMYSFEDRGGDSLT
LRPEFTAGIARAFLTNGWQQHAPLKVATHGPLFRYERPQKGRYRQFHQIDAEIIGAAEPQADVELLAMADQTIRGLGIEG
VTLHLNTLGDAESREAWRAALVEYFRAVASELSEDSQERLEKNPLRILDSKDRRDQQFLADAPRIDAFLSDTARAFFESV
TTGLDAAGVKWQRAESLVRGLDYYRHTAFEFIPDEGSEAAGKLGSQSTILGGGRYDGLMESLGGAPTPAVGWAAGIERLA
MLVGGAGFDPPIVVLAEHEHLFDTARLVVQALRLSEITAEAEFRYRRTNKAFDKFKKTGTDVFLVVDQVANLNGSHQIDI
RLSGVLPDEEEELKLLAKRVELSLRLFFPDLIPKADPEKQAISWYLSRK

Sequences:

>Translated_449_residues
MSKKTPQAIRGTQDIFSADAEAFAFVVETFERVRKLYRFRRVEMPVFEKTEVFSRAIGETTDVVSKEMYSFEDRGGDSLT
LRPEFTAGIARAFLTNGWQQHAPLKVATHGPLFRYERPQKGRYRQFHQIDAEIIGAAEPQADVELLAMADQTIRGLGIEG
VTLHLNTLGDAESREAWRAALVEYFRAVASELSEDSQERLEKNPLRILDSKDRRDQQFLADAPRIDAFLSDTARAFFESV
TTGLDAAGVKWQRAESLVRGLDYYRHTAFEFIPDEGSEAAGKLGSQSTILGGGRYDGLMESLGGAPTPAVGWAAGIERLA
MLVGGAGFDPPIVVLAEHEHLFDTARLVVQALRLSEITAEAEFRYRRTNKAFDKFKKTGTDVFLVVDQVANLNGSHQIDI
RLSGVLPDEEEELKLLAKRVELSLRLFFPDLIPKADPEKQAISWYLSRK
>Mature_448_residues
SKKTPQAIRGTQDIFSADAEAFAFVVETFERVRKLYRFRRVEMPVFEKTEVFSRAIGETTDVVSKEMYSFEDRGGDSLTL
RPEFTAGIARAFLTNGWQQHAPLKVATHGPLFRYERPQKGRYRQFHQIDAEIIGAAEPQADVELLAMADQTIRGLGIEGV
TLHLNTLGDAESREAWRAALVEYFRAVASELSEDSQERLEKNPLRILDSKDRRDQQFLADAPRIDAFLSDTARAFFESVT
TGLDAAGVKWQRAESLVRGLDYYRHTAFEFIPDEGSEAAGKLGSQSTILGGGRYDGLMESLGGAPTPAVGWAAGIERLAM
LVGGAGFDPPIVVLAEHEHLFDTARLVVQALRLSEITAEAEFRYRRTNKAFDKFKKTGTDVFLVVDQVANLNGSHQIDIR
LSGVLPDEEEELKLLAKRVELSLRLFFPDLIPKADPEKQAISWYLSRK

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=321, Percent_Identity=46.1059190031153, Blast_Score=288, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6325290, Length=480, Percent_Identity=25.4166666666667, Blast_Score=87, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_ERYLH (Q2N5U7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459741.1
- STRING:   Q2N5U7
- GeneID:   3870862
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_14260
- NMPDR:   fig|314225.3.peg.166
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   IERSINC
- PhylomeDB:   Q2N5U7
- BioCyc:   ELIT314225:ELI_14260-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR015807
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: =6.1.1.21

Molecular weight: Translated: 50263; Mature: 50132

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKTPQAIRGTQDIFSADAEAFAFVVETFERVRKLYRFRRVEMPVFEKTEVFSRAIGET
CCCCCCHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCH
TDVVSKEMYSFEDRGGDSLTLRPEFTAGIARAFLTNGWQQHAPLKVATHGPLFRYERPQK
HHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCHHHCCCEEEECCCCCCCCCCCCC
GRYRQFHQIDAEIIGAAEPQADVELLAMADQTIRGLGIEGVTLHLNTLGDAESREAWRAA
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
LVEYFRAVASELSEDSQERLEKNPLRILDSKDRRDQQFLADAPRIDAFLSDTARAFFESV
HHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
TTGLDAAGVKWQRAESLVRGLDYYRHTAFEFIPDEGSEAAGKLGSQSTILGGGRYDGLME
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEECCCHHHHHHH
SLGGAPTPAVGWAAGIERLAMLVGGAGFDPPIVVLAEHEHLFDTARLVVQALRLSEITAE
HCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AEFRYRRTNKAFDKFKKTGTDVFLVVDQVANLNGSHQIDIRLSGVLPDEEEELKLLAKRV
HHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
ELSLRLFFPDLIPKADPEKQAISWYLSRK
HHHHHEECHHHCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
SKKTPQAIRGTQDIFSADAEAFAFVVETFERVRKLYRFRRVEMPVFEKTEVFSRAIGET
CCCCCHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCH
TDVVSKEMYSFEDRGGDSLTLRPEFTAGIARAFLTNGWQQHAPLKVATHGPLFRYERPQK
HHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCHHHCCCEEEECCCCCCCCCCCCC
GRYRQFHQIDAEIIGAAEPQADVELLAMADQTIRGLGIEGVTLHLNTLGDAESREAWRAA
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
LVEYFRAVASELSEDSQERLEKNPLRILDSKDRRDQQFLADAPRIDAFLSDTARAFFESV
HHHHHHHHHHHHCCCHHHHHHCCCCEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
TTGLDAAGVKWQRAESLVRGLDYYRHTAFEFIPDEGSEAAGKLGSQSTILGGGRYDGLME
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCEEECCCHHHHHHH
SLGGAPTPAVGWAAGIERLAMLVGGAGFDPPIVVLAEHEHLFDTARLVVQALRLSEITAE
HCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AEFRYRRTNKAFDKFKKTGTDVFLVVDQVANLNGSHQIDIRLSGVLPDEEEELKLLAKRV
HHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHH
ELSLRLFFPDLIPKADPEKQAISWYLSRK
HHHHHEECHHHCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA