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The map label for this gene is prfA

Identifier: 85375680

GI number: 85375680

Start: 2864723

End: 2865790

Strand: Direct

Name: prfA

Synonym: ELI_14265

Alternate gene names: 85375680

Gene position: 2864723-2865790 (Clockwise)

Preceding gene: 85375679

Following gene: 85375681

Centisome position: 93.85

GC content: 65.36

Gene sequence:

>1068_bases
ATGACTATCCCCGCCACCCGCCTCGACCAGATCGCCAACCGCTTCGCCGAGCTTGAAGCGCGGATGGCATCGGGCACGCT
GGAAGGCGAGGAATTCGTGCAGGCGAGCCGCGACTATGCCGAGTTGGAGCCGGTCGCCAAGGTCGCCGCGGAAGTGAAGG
CCATGCGCGAGGAGATCGGCGGCTTGGAGGAAATGCTCGCCGATCCCGAGATGAAGGCGATGGCGCAGGAAGAGCTCGCC
GCCATTCGCGAGGCGCTTCCGGAAAAGGAACGCCAGCTCGCCATCGCCATGCTGCCGAAGGACAGCGCCGACAACAAGCC
TGCGATGCTGGAAATCCGTGCCGGGACCGGCGGCGATGAAGCGGCGCTGTTTGCGGGCGATCTGTATCGCATGTACGAGC
GCTTTGCTGGCGAGCAGGGCTGGAAGGTCGAACCGGTCAGCATGAACGCCTCCGAAGTCGGCGGTTTCAAGGAAATTGTC
GCCAATGTCAGCGGCACCGGGGTCTTCGCCAAGCTCAAGTTCGAAAGCGGCGTTCACCGCGTCCAGCGCGTGCCCGAGAC
GGAGAGCGGCGGGCGCATCCACACCAGCGCGGCGACCGTGGCGGTGCTGCCCGAACCAGACGAAGTCGATGTCCAGATCG
ACGACAAGGATCTGAAGATCGACATCTACCGCGCCAGCGGCGCGGGCGGCCAGCACGTCAACACCACCGACAGCGCGGTG
CGCATCACGCATCTGCCGACCGGCATCGTCGTCCAGCAACAGGACGAGCGCAGCCAGCACAAGAACAAGGCCAAGGCGAT
GCAGGTCCTGCGCACACGGCTCTACGACCACTTGCGCGAGCAGTCGCAGGGCGAGGAAGCCGCCGCGCGCAAGGCGATGG
TCGGCAGCGGCGATCGCTCCGAACGCATTCGCACCTATAATTTTCCGCAAGGCCGCGTGACCGATCACCGCATCGGCCTG
ACTCTCCACAAGCTCGACGAGATCATTGCCGGGCCGGGGCTTGGCGAACTGGTGGGCGCGCTCATCGCCGAGGATGAAGC
CAAGCGGCTGGCGGCGCTTTCGGAGTGA

Upstream 100 bases:

>100_bases
TGGCCAAGAGAGTTGAATTAAGCCTCCGATTGTTCTTTCCCGACCTAATACCGAAGGCTGATCCTGAGAAGCAAGCCATC
TCTTGGTATCTCTCTCGCAA

Downstream 100 bases:

>100_bases
GGGAGGCGAGCGTTACGGCTGCGCTTCGACGGGCTCGGGGCGAACGCAGGGAGCGCCGGCGGCTCCTCTCTACGTCCGTT
CGTGCTGAGCCTGTCGAAGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTIPATRLDQIANRFAELEARMASGTLEGEEFVQASRDYAELEPVAKVAAEVKAMREEIGGLEEMLADPEMKAMAQEELA
AIREALPEKERQLAIAMLPKDSADNKPAMLEIRAGTGGDEAALFAGDLYRMYERFAGEQGWKVEPVSMNASEVGGFKEIV
ANVSGTGVFAKLKFESGVHRVQRVPETESGGRIHTSAATVAVLPEPDEVDVQIDDKDLKIDIYRASGAGGQHVNTTDSAV
RITHLPTGIVVQQQDERSQHKNKAKAMQVLRTRLYDHLREQSQGEEAAARKAMVGSGDRSERIRTYNFPQGRVTDHRIGL
TLHKLDEIIAGPGLGELVGALIAEDEAKRLAALSE

Sequences:

>Translated_355_residues
MTIPATRLDQIANRFAELEARMASGTLEGEEFVQASRDYAELEPVAKVAAEVKAMREEIGGLEEMLADPEMKAMAQEELA
AIREALPEKERQLAIAMLPKDSADNKPAMLEIRAGTGGDEAALFAGDLYRMYERFAGEQGWKVEPVSMNASEVGGFKEIV
ANVSGTGVFAKLKFESGVHRVQRVPETESGGRIHTSAATVAVLPEPDEVDVQIDDKDLKIDIYRASGAGGQHVNTTDSAV
RITHLPTGIVVQQQDERSQHKNKAKAMQVLRTRLYDHLREQSQGEEAAARKAMVGSGDRSERIRTYNFPQGRVTDHRIGL
TLHKLDEIIAGPGLGELVGALIAEDEAKRLAALSE
>Mature_354_residues
TIPATRLDQIANRFAELEARMASGTLEGEEFVQASRDYAELEPVAKVAAEVKAMREEIGGLEEMLADPEMKAMAQEELAA
IREALPEKERQLAIAMLPKDSADNKPAMLEIRAGTGGDEAALFAGDLYRMYERFAGEQGWKVEPVSMNASEVGGFKEIVA
NVSGTGVFAKLKFESGVHRVQRVPETESGGRIHTSAATVAVLPEPDEVDVQIDDKDLKIDIYRASGAGGQHVNTTDSAVR
ITHLPTGIVVQQQDERSQHKNKAKAMQVLRTRLYDHLREQSQGEEAAARKAMVGSGDRSERIRTYNFPQGRVTDHRIGLT
LHKLDEIIAGPGLGELVGALIAEDEAKRLAALSE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=300, Percent_Identity=46, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI34577120, Length=354, Percent_Identity=39.8305084745763, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI166795305, Length=206, Percent_Identity=42.7184466019417, Blast_Score=185, Evalue=6e-47,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=55.6818181818182, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI2367172, Length=295, Percent_Identity=38.9830508474576, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=337, Percent_Identity=37.3887240356083, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6321295, Length=333, Percent_Identity=43.8438438438438, Blast_Score=262, Evalue=7e-71,
Organism=Drosophila melanogaster, GI19921226, Length=319, Percent_Identity=43.5736677115987, Blast_Score=247, Evalue=8e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_ERYLH (Q2N5U6)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459742.1
- ProteinModelPortal:   Q2N5U6
- SMR:   Q2N5U6
- STRING:   Q2N5U6
- GeneID:   3870125
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_14265
- NMPDR:   fig|314225.3.peg.165
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q2N5U6
- ProtClustDB:   PRK00591
- BioCyc:   ELIT314225:ELI_14265-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38725; Mature: 38594

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPATRLDQIANRFAELEARMASGTLEGEEFVQASRDYAELEPVAKVAAEVKAMREEIG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GLEEMLADPEMKAMAQEELAAIREALPEKERQLAIAMLPKDSADNKPAMLEIRAGTGGDE
CHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCCCCCEEEEEECCCCCCC
AALFAGDLYRMYERFAGEQGWKVEPVSMNASEVGGFKEIVANVSGTGVFAKLKFESGVHR
HHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCHHHHHHCCCCCCEEEEEEHHHHHHH
VQRVPETESGGRIHTSAATVAVLPEPDEVDVQIDDKDLKIDIYRASGAGGQHVNTTDSAV
HHHCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCEE
RITHLPTGIVVQQQDERSQHKNKAKAMQVLRTRLYDHLREQSQGEEAAARKAMVGSGDRS
EEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
ERIRTYNFPQGRVTDHRIGLTLHKLDEIIAGPGLGELVGALIAEDEAKRLAALSE
CCCEEECCCCCCCCCHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIPATRLDQIANRFAELEARMASGTLEGEEFVQASRDYAELEPVAKVAAEVKAMREEIG
CCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GLEEMLADPEMKAMAQEELAAIREALPEKERQLAIAMLPKDSADNKPAMLEIRAGTGGDE
CHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHCEEEEECCCCCCCCCCEEEEEECCCCCCC
AALFAGDLYRMYERFAGEQGWKVEPVSMNASEVGGFKEIVANVSGTGVFAKLKFESGVHR
HHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCHHHHHHCCCCCCEEEEEEHHHHHHH
VQRVPETESGGRIHTSAATVAVLPEPDEVDVQIDDKDLKIDIYRASGAGGQHVNTTDSAV
HHHCCCCCCCCEEEECCEEEEECCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCEE
RITHLPTGIVVQQQDERSQHKNKAKAMQVLRTRLYDHLREQSQGEEAAARKAMVGSGDRS
EEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
ERIRTYNFPQGRVTDHRIGLTLHKLDEIIAGPGLGELVGALIAEDEAKRLAALSE
CCCEEECCCCCCCCCHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA