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The map label for this gene is prs [H]

Identifier: 85375585

GI number: 85375585

Start: 2778038

End: 2778973

Strand: Direct

Name: prs [H]

Synonym: ELI_13790

Alternate gene names: 85375585

Gene position: 2778038-2778973 (Clockwise)

Preceding gene: 85375584

Following gene: 85375588

Centisome position: 91.01

GC content: 66.35

Gene sequence:

>936_bases
ATGAAGATCATGGCCGGCAATTCGAACCTGCCGCTCGCCCGGGCGATTGCCGCCTATCTCGAAATCCCGCTTACCGATGC
CAGCGTGCGGCGGTTCGCGGACGAGGAAATCTTCATCGAGATCCACGAGAACGTGCGCGGCGAGGATGTCTTCCTGGTCC
AGTCGACCAGCTATCCGGCCAACGACAATCTGATGGAACTGCTGATCGGGATCGACGCGCTGCGCCGCGCCTCGGCCAAG
CGGATCACCGCCGTAATTCCCTATTTCGGCTACGCCCGGCAGGACCGGAAGCCCGGCCCGCGTACGCCGATTTCGGCCAA
GCTTGTCGCCAATCTCATCACCGAGGCGGGGGCCGACCGCGTGCTGGCGGTGGACCTGCACGCCGGGCAGATCCAGGGCT
TCTTCGATATCCCGACCGACAACCTCTACGCCGCGCCGACCATGGCGGCCGATATCCAGGCCCGCTACGGCGACCGCGAC
CTGATGGTGGTAAGCCCCGATGTCGGCGGCGTCGTCCGCGCCCGCGCGCTCGCCAAGCGGCTCGACAACGCGCCGCTCGC
CATCGTCGACAAGCGCCGCGATCGGCCCGGCGAAAGCGAAGTCATGAACATCATTGGCGACGTGAAGGGCCGCCACTGCG
TGCTGATCGACGACATCGTCGATTCGGGCGGCACGCTGTGCAACGCGGCCGAGGCGCTGCTCGAACAGGGCGCGACTTCG
GTTGCCGCCTATATCACTCATGGTGTCCTCTCGGGCGGTGCCGTCGCCCGGGTCAACGGCTCGGCGCTGACCGAACTGGT
CGTGACGGACACGATCGGCGCGACAGAAGCGGCGGGCGATGCGGAAAAGATCCGCTACCTCACCATCGCCCCGCTGATCG
GCGAGGCGGTGCGCCGCATCGCCGACGAAAGTTCGGTCAGCTCGCTGTTCGACTGA

Upstream 100 bases:

>100_bases
GCAACCGCCACACCCCATGTTGCGTTTCGATGACGCGCGTCTAAGCCGAAAGCGACCGGGATCGCGCGTCGATTCGCACC
AGAACTGGGGACCAGCACAC

Downstream 100 bases:

>100_bases
TCAGCGCGGTCACTGCCACGGGCGCTTGCCGCCCTGCGCGATCCGCTCCAGTTCTTCCAGCCGCCGCGCCTGCCGCGATT
CCAGCGTGACGGTCAGCAGC

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKIMAGNSNLPLARAIAAYLEIPLTDASVRRFADEEIFIEIHENVRGEDVFLVQSTSYPANDNLMELLIGIDALRRASAK
RITAVIPYFGYARQDRKPGPRTPISAKLVANLITEAGADRVLAVDLHAGQIQGFFDIPTDNLYAAPTMAADIQARYGDRD
LMVVSPDVGGVVRARALAKRLDNAPLAIVDKRRDRPGESEVMNIIGDVKGRHCVLIDDIVDSGGTLCNAAEALLEQGATS
VAAYITHGVLSGGAVARVNGSALTELVVTDTIGATEAAGDAEKIRYLTIAPLIGEAVRRIADESSVSSLFD

Sequences:

>Translated_311_residues
MKIMAGNSNLPLARAIAAYLEIPLTDASVRRFADEEIFIEIHENVRGEDVFLVQSTSYPANDNLMELLIGIDALRRASAK
RITAVIPYFGYARQDRKPGPRTPISAKLVANLITEAGADRVLAVDLHAGQIQGFFDIPTDNLYAAPTMAADIQARYGDRD
LMVVSPDVGGVVRARALAKRLDNAPLAIVDKRRDRPGESEVMNIIGDVKGRHCVLIDDIVDSGGTLCNAAEALLEQGATS
VAAYITHGVLSGGAVARVNGSALTELVVTDTIGATEAAGDAEKIRYLTIAPLIGEAVRRIADESSVSSLFD
>Mature_311_residues
MKIMAGNSNLPLARAIAAYLEIPLTDASVRRFADEEIFIEIHENVRGEDVFLVQSTSYPANDNLMELLIGIDALRRASAK
RITAVIPYFGYARQDRKPGPRTPISAKLVANLITEAGADRVLAVDLHAGQIQGFFDIPTDNLYAAPTMAADIQARYGDRD
LMVVSPDVGGVVRARALAKRLDNAPLAIVDKRRDRPGESEVMNIIGDVKGRHCVLIDDIVDSGGTLCNAAEALLEQGATS
VAAYITHGVLSGGAVARVNGSALTELVVTDTIGATEAAGDAEKIRYLTIAPLIGEAVRRIADESSVSSLFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=311, Percent_Identity=47.2668810289389, Blast_Score=288, Evalue=5e-78,
Organism=Homo sapiens, GI4506129, Length=309, Percent_Identity=45.9546925566343, Blast_Score=282, Evalue=3e-76,
Organism=Homo sapiens, GI28557709, Length=311, Percent_Identity=46.3022508038585, Blast_Score=280, Evalue=8e-76,
Organism=Homo sapiens, GI84875539, Length=312, Percent_Identity=45.5128205128205, Blast_Score=276, Evalue=1e-74,
Organism=Homo sapiens, GI4506133, Length=342, Percent_Identity=34.5029239766082, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI194018537, Length=342, Percent_Identity=32.4561403508772, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=29.8611111111111, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=29.8611111111111, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=29.8611111111111, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=29.8611111111111, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=54.4871794871795, Blast_Score=339, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25149168, Length=311, Percent_Identity=45.3376205787781, Blast_Score=278, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17554702, Length=311, Percent_Identity=45.3376205787781, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71989924, Length=311, Percent_Identity=45.3376205787781, Blast_Score=276, Evalue=7e-75,
Organism=Caenorhabditis elegans, GI17554704, Length=309, Percent_Identity=45.3074433656958, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17570245, Length=337, Percent_Identity=33.5311572700297, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319403, Length=313, Percent_Identity=45.6869009584665, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6320946, Length=312, Percent_Identity=43.5897435897436, Blast_Score=258, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6321776, Length=311, Percent_Identity=44.0514469453376, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6322667, Length=196, Percent_Identity=42.8571428571429, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324511, Length=110, Percent_Identity=37.2727272727273, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21355239, Length=311, Percent_Identity=46.9453376205788, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI45551540, Length=334, Percent_Identity=43.7125748502994, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24651458, Length=352, Percent_Identity=32.9545454545455, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24651456, Length=352, Percent_Identity=32.9545454545455, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI281362873, Length=352, Percent_Identity=32.9545454545455, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24651454, Length=352, Percent_Identity=32.9545454545455, Blast_Score=177, Evalue=7e-45,
Organism=Drosophila melanogaster, GI24651462, Length=371, Percent_Identity=32.0754716981132, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24651464, Length=371, Percent_Identity=32.0754716981132, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45552010, Length=371, Percent_Identity=32.0754716981132, Blast_Score=170, Evalue=1e-42,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33194; Mature: 33194

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIMAGNSNLPLARAIAAYLEIPLTDASVRRFADEEIFIEIHENVRGEDVFLVQSTSYPA
CEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCC
NDNLMELLIGIDALRRASAKRITAVIPYFGYARQDRKPGPRTPISAKLVANLITEAGADR
CCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
VLAVDLHAGQIQGFFDIPTDNLYAAPTMAADIQARYGDRDLMVVSPDVGGVVRARALAKR
EEEEEECCCCCCEEEECCCCCEEECCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
LDNAPLAIVDKRRDRPGESEVMNIIGDVKGRHCVLIDDIVDSGGTLCNAAEALLEQGATS
HCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHCCCHH
VAAYITHGVLSGGAVARVNGSALTELVVTDTIGATEAAGDAEKIRYLTIAPLIGEAVRRI
HHHHHHHHHHCCCEEEEECCHHHHHEEEECCCCCCCCCCCHHHEEEEEEHHHHHHHHHHH
ADESSVSSLFD
HCHHHHHHHCC
>Mature Secondary Structure
MKIMAGNSNLPLARAIAAYLEIPLTDASVRRFADEEIFIEIHENVRGEDVFLVQSTSYPA
CEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEEEEEECCCCCCEEEEEECCCCCC
NDNLMELLIGIDALRRASAKRITAVIPYFGYARQDRKPGPRTPISAKLVANLITEAGADR
CCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
VLAVDLHAGQIQGFFDIPTDNLYAAPTMAADIQARYGDRDLMVVSPDVGGVVRARALAKR
EEEEEECCCCCCEEEECCCCCEEECCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHH
LDNAPLAIVDKRRDRPGESEVMNIIGDVKGRHCVLIDDIVDSGGTLCNAAEALLEQGATS
HCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHCCCHH
VAAYITHGVLSGGAVARVNGSALTELVVTDTIGATEAAGDAEKIRYLTIAPLIGEAVRRI
HHHHHHHHHHCCCEEEEECCHHHHHEEEECCCCCCCCCCCHHHEEEEEEHHHHHHHHHHH
ADESSVSSLFD
HCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]