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The map label for this gene is ppx [H]

Identifier: 85374882

GI number: 85374882

Start: 2085864

End: 2087153

Strand: Direct

Name: ppx [H]

Synonym: ELI_10275

Alternate gene names: 85374882

Gene position: 2085864-2087153 (Clockwise)

Preceding gene: 85374878

Following gene: 85374883

Centisome position: 68.34

GC content: 65.5

Gene sequence:

>1290_bases
ATGTCGGTGCGAGAAGGTAATGAAAAGTCGAAAAACATGGCGGAAATGTCTCCGCCGGACGGTAACAGGAGGGCAGGCCG
CAAGGGGAACAAAAGGTCCGGCACAGTAGCCGATTTTCGCTACAAGAGTTCCCCGCCGCCCGAAACCAAGTCGAAAGAAC
GTCGCGCCCGCCGCGACGGTGCTTCCCGCAATCGCAACAATGGCAAGGCCGCAGAGCCTGCCTCGGCACGCGCCGCGCGT
TCGCCGCGGCCCATGCACAAGCCGCATGGCGGACCGCGCTATCGGCAGGCCTATGCCGCACTCGACCTCGGTACCAACAA
TTGCCGGCTGTTGATCGCGCGACCTTCGGGCGAGAACTTCACCGTCATCGACGCCTTCAGCCGCGTCGTGAAGCTGGGCG
AGGGCCTCGCGCATAGCGGGCGGCTCTCGGACGATGCGATGGACCGCGCGCTGGGCGCGCTCAAAGTCTGTTCCGACAAG
CTGCGCCGCCGCAACGTCTATCTCGCCCGCTCGGTCGCGACCGAGGCCTGTCGCCGCGCCAGCAATGGCGAAGACTTCAT
CCAGCGGGTGAAGGACGAAACCGGCATCGTACTCGACATCATTACCGCGGAAGAAGAAGCCCGGCTGGCGGTGCTCGGCT
GCCATGTCCTGCTGGAACAGGGCGAGGGCCGCGCTGTCATCTTCGACATCGGCGGCGGATCGACCGAACTGGTCCTGATC
GAACCCAATGGCCGCGGCGTGCCCCGCATTCTCGACTGGCAGAGCGTTCCGTGGGGCGTCGTCTCGCTGTCCGACACCGT
TGCCAAGACCGATCGCGGGCAGGAGGCCAGGCTCGCGCGCTACGCCGAAATGCGCCGGGTCGTCGGCGAGAGCTTTACGC
CATTTGCCGAGCGGGTGGCGCAGCATGCCGGGCAGCCCGACATCCGCCTGCTCGGCACAAGCGGCACGGTTACGACCCTA
GCCAGCCTTCATCTCGAATTGCCGCAATACGACCGCCGCGCGGTCGACGGGCTGATCGTGCCCTCGCAGGTAATGCGCGA
TATCTCGTTGCGGCTGTCGGCCATGAGCGTGGACGAACGCTGCACCCTGCCCTGCATCGGGCAGGATCGCGCCGAACTGG
TGGTGGCCGGATGCGCCATCCTCGAATCGATTATCGACCTGTGGCCCGCCGAAAAGCTGGGCGTTGCCGATCGCGGCATC
CGCGAAGGCATCCTGCGCAGCCTGATGGCGGGCGATTTCGCCACCGCGCGCGATCGCAAGGCGATGGACGAAACCCGGAA
AGCACAATGA

Upstream 100 bases:

>100_bases
CCTGCCCCGTTTGCGTCAAGGCTGCTTCGACGCCTACCGGACACCCAACTCTTGCCGTATCGTGCGCTCTCGCCTAACTT
CATTTTCAGGTCCTCGCGCT

Downstream 100 bases:

>100_bases
GCCGGTCCGGCAAAGATCCAGGCAAGCGGGTCAAGACGGCGCGCAAGAGATCGGCCTCCTCGACCCGTTGGCTCGAGCGC
CAGCTAAACGATCCCTATGT

Product: exopolyphosphatase

Products: NA

Alternate protein names: ExopolyPase; Metaphosphatase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSVREGNEKSKNMAEMSPPDGNRRAGRKGNKRSGTVADFRYKSSPPPETKSKERRARRDGASRNRNNGKAAEPASARAAR
SPRPMHKPHGGPRYRQAYAALDLGTNNCRLLIARPSGENFTVIDAFSRVVKLGEGLAHSGRLSDDAMDRALGALKVCSDK
LRRRNVYLARSVATEACRRASNGEDFIQRVKDETGIVLDIITAEEEARLAVLGCHVLLEQGEGRAVIFDIGGGSTELVLI
EPNGRGVPRILDWQSVPWGVVSLSDTVAKTDRGQEARLARYAEMRRVVGESFTPFAERVAQHAGQPDIRLLGTSGTVTTL
ASLHLELPQYDRRAVDGLIVPSQVMRDISLRLSAMSVDERCTLPCIGQDRAELVVAGCAILESIIDLWPAEKLGVADRGI
REGILRSLMAGDFATARDRKAMDETRKAQ

Sequences:

>Translated_429_residues
MSVREGNEKSKNMAEMSPPDGNRRAGRKGNKRSGTVADFRYKSSPPPETKSKERRARRDGASRNRNNGKAAEPASARAAR
SPRPMHKPHGGPRYRQAYAALDLGTNNCRLLIARPSGENFTVIDAFSRVVKLGEGLAHSGRLSDDAMDRALGALKVCSDK
LRRRNVYLARSVATEACRRASNGEDFIQRVKDETGIVLDIITAEEEARLAVLGCHVLLEQGEGRAVIFDIGGGSTELVLI
EPNGRGVPRILDWQSVPWGVVSLSDTVAKTDRGQEARLARYAEMRRVVGESFTPFAERVAQHAGQPDIRLLGTSGTVTTL
ASLHLELPQYDRRAVDGLIVPSQVMRDISLRLSAMSVDERCTLPCIGQDRAELVVAGCAILESIIDLWPAEKLGVADRGI
REGILRSLMAGDFATARDRKAMDETRKAQ
>Mature_428_residues
SVREGNEKSKNMAEMSPPDGNRRAGRKGNKRSGTVADFRYKSSPPPETKSKERRARRDGASRNRNNGKAAEPASARAARS
PRPMHKPHGGPRYRQAYAALDLGTNNCRLLIARPSGENFTVIDAFSRVVKLGEGLAHSGRLSDDAMDRALGALKVCSDKL
RRRNVYLARSVATEACRRASNGEDFIQRVKDETGIVLDIITAEEEARLAVLGCHVLLEQGEGRAVIFDIGGGSTELVLIE
PNGRGVPRILDWQSVPWGVVSLSDTVAKTDRGQEARLARYAEMRRVVGESFTPFAERVAQHAGQPDIRLLGTSGTVTTLA
SLHLELPQYDRRAVDGLIVPSQVMRDISLRLSAMSVDERCTLPCIGQDRAELVVAGCAILESIIDLWPAEKLGVADRGIR
EGILRSLMAGDFATARDRKAMDETRKAQ

Specific function: Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate [H]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=340, Percent_Identity=29.4117647058824, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI48994966, Length=315, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022371
- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: =3.6.1.11 [H]

Molecular weight: Translated: 46901; Mature: 46770

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVREGNEKSKNMAEMSPPDGNRRAGRKGNKRSGTVADFRYKSSPPPETKSKERRARRDG
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCC
ASRNRNNGKAAEPASARAARSPRPMHKPHGGPRYRQAYAALDLGTNNCRLLIARPSGENF
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHEECCCCCCEEEEECCCCCCE
TVIDAFSRVVKLGEGLAHSGRLSDDAMDRALGALKVCSDKLRRRNVYLARSVATEACRRA
EHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNGEDFIQRVKDETGIVLDIITAEEEARLAVLGCHVLLEQGEGRAVIFDIGGGSTELVLI
CCCHHHHHHHHCCCCEEEEEEECCCCCCEEEEEHHHHEECCCCCEEEEECCCCCEEEEEE
EPNGRGVPRILDWQSVPWGVVSLSDTVAKTDRGQEARLARYAEMRRVVGESFTPFAERVA
CCCCCCCCCEECCCCCCCCEEECCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
QHAGQPDIRLLGTSGTVTTLASLHLELPQYDRRAVDGLIVPSQVMRDISLRLSAMSVDER
HHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHCCEECCHHHHHHHHHHHHHCCCCCC
CTLPCIGQDRAELVVAGCAILESIIDLWPAEKLGVADRGIREGILRSLMAGDFATARDRK
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHHH
AMDETRKAQ
HHHHHHCCC
>Mature Secondary Structure 
SVREGNEKSKNMAEMSPPDGNRRAGRKGNKRSGTVADFRYKSSPPPETKSKERRARRDG
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCC
ASRNRNNGKAAEPASARAARSPRPMHKPHGGPRYRQAYAALDLGTNNCRLLIARPSGENF
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHEECCCCCCEEEEECCCCCCE
TVIDAFSRVVKLGEGLAHSGRLSDDAMDRALGALKVCSDKLRRRNVYLARSVATEACRRA
EHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNGEDFIQRVKDETGIVLDIITAEEEARLAVLGCHVLLEQGEGRAVIFDIGGGSTELVLI
CCCHHHHHHHHCCCCEEEEEEECCCCCCEEEEEHHHHEECCCCCEEEEECCCCCEEEEEE
EPNGRGVPRILDWQSVPWGVVSLSDTVAKTDRGQEARLARYAEMRRVVGESFTPFAERVA
CCCCCCCCCEECCCCCCCCEEECCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
QHAGQPDIRLLGTSGTVTTLASLHLELPQYDRRAVDGLIVPSQVMRDISLRLSAMSVDER
HHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHCCEECCHHHHHHHHHHHHHCCCCCC
CTLPCIGQDRAELVVAGCAILESIIDLWPAEKLGVADRGIREGILRSLMAGDFATARDRK
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHHH
AMDETRKAQ
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]